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The map label for this gene is pilT-3 [H]

Identifier: 39995544

GI number: 39995544

Start: 467785

End: 468855

Strand: Direct

Name: pilT-3 [H]

Synonym: GSU0436

Alternate gene names: 39995544

Gene position: 467785-468855 (Clockwise)

Preceding gene: 39995543

Following gene: 39995545

Centisome position: 12.26

GC content: 62.56

Gene sequence:

>1071_bases
ATGGCACGTATCGACGCACTGTTCAAGCTGCTGAAGGAGCAAGGGGCCTCCGACCTCCACCTGTCGTCCGGGGCTCCGCC
CATTTTCAGGCTTCACGGAGAGATGGCGCGGCAGAACTTCAAGGTTCTTTCCCACGAGGAACTGACGGCCATCCTCTACG
AAATCCTGACCGACAAGCAGAAGGCCGATTTCGAGGAGCGGCGGGACCTGGACTTCGCCTATGCCATTCCCGGCCTGGCC
CGGTTCCGGGGGAACTACATGATGACCCACCGGGGCATCGCCGCGGTCTTCCGGATCATCCCCAGCAAGATCCTCTCGGC
AGACGACCTGAGCCTTCCCGACGGGGTTCGCCGCATGACCCAGTTCAAGAAGGGGCTCGTGCTGGTCACCGGGCCCACGG
GGTCGGGCAAGTCCACCACCCTTGCCGCCATGATCGACCTCATCAACGCCACCCGCAAGGAGCACATCCTCACTCTGGAA
GATCCTCTCGAATTCATCCACGAGAACAAGATGTCGCTCCTGAACCAGCGGCAGATCGGCGAGCACTCCCTGAGTTTTTC
CGCCGCCCTTCGCGCCGCCCTGCGGGAGGACCCCGACGTGATCCTCGTGGGCGAGATGCGGGACCTGGAGACCATAGGCC
TCGCCATGAGCGCAGCCGAGACCGGCCACCTGGTCTTCGGCACCCTCCATACCAACTCGGCGGCCAAGACCATCGACCGG
ATCATCGACGTTTTTCCCACCGATCAGCAGGAGCAGACCCGGGCCATGCTCTCCGAATCTCTCAAAGGGGTCGTCTGCCA
GCAGCTTCTCAAAACCGCCGACGGCAAGGGGCGGGTTGCGGCACTGGAGATCATGCTCGGCACACCGGCCATCGCCAACC
TGATCAGGGAGGGGAAAACCTTCCAGATCCCCTCCATCATCCAGACTGCCAAGCGTGACGGCATGCAGCTCATGGACCAG
CACCTGCTGGACCTCTTCAAGACCAAGCGGATCACCGCCGAAGAGGCCTACCGCTGCGCCCAGGACAAGAAGCAGTTCGA
GCAGTACCTGACGGAAAAGCCGGGCCAGTAG

Upstream 100 bases:

>100_bases
TGGCGCGGGATCACGGACGAGCGGCAGACCCTGCTGGCTGAAGGCACTATTTCGCTGGAGGAGTACGCCTCCTCCCTGCA
CGGTTGACGGAGACGGTTTC

Downstream 100 bases:

>100_bases
GATGAAAAAAACCTTGCCAGAGCAGCAGGTCGCATGATATATAAATTGCTTCTTAGTCGGGGCGTAGCGCAGCCTGGTAG
CGCACCTGCTTCGGGAGCAG

Product: twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MARIDALFKLLKEQGASDLHLSSGAPPIFRLHGEMARQNFKVLSHEELTAILYEILTDKQKADFEERRDLDFAYAIPGLA
RFRGNYMMTHRGIAAVFRIIPSKILSADDLSLPDGVRRMTQFKKGLVLVTGPTGSGKSTTLAAMIDLINATRKEHILTLE
DPLEFIHENKMSLLNQRQIGEHSLSFSAALRAALREDPDVILVGEMRDLETIGLAMSAAETGHLVFGTLHTNSAAKTIDR
IIDVFPTDQQEQTRAMLSESLKGVVCQQLLKTADGKGRVAALEIMLGTPAIANLIREGKTFQIPSIIQTAKRDGMQLMDQ
HLLDLFKTKRITAEEAYRCAQDKKQFEQYLTEKPGQ

Sequences:

>Translated_356_residues
MARIDALFKLLKEQGASDLHLSSGAPPIFRLHGEMARQNFKVLSHEELTAILYEILTDKQKADFEERRDLDFAYAIPGLA
RFRGNYMMTHRGIAAVFRIIPSKILSADDLSLPDGVRRMTQFKKGLVLVTGPTGSGKSTTLAAMIDLINATRKEHILTLE
DPLEFIHENKMSLLNQRQIGEHSLSFSAALRAALREDPDVILVGEMRDLETIGLAMSAAETGHLVFGTLHTNSAAKTIDR
IIDVFPTDQQEQTRAMLSESLKGVVCQQLLKTADGKGRVAALEIMLGTPAIANLIREGKTFQIPSIIQTAKRDGMQLMDQ
HLLDLFKTKRITAEEAYRCAQDKKQFEQYLTEKPGQ
>Mature_355_residues
ARIDALFKLLKEQGASDLHLSSGAPPIFRLHGEMARQNFKVLSHEELTAILYEILTDKQKADFEERRDLDFAYAIPGLAR
FRGNYMMTHRGIAAVFRIIPSKILSADDLSLPDGVRRMTQFKKGLVLVTGPTGSGKSTTLAAMIDLINATRKEHILTLED
PLEFIHENKMSLLNQRQIGEHSLSFSAALRAALREDPDVILVGEMRDLETIGLAMSAAETGHLVFGTLHTNSAAKTIDRI
IDVFPTDQQEQTRAMLSESLKGVVCQQLLKTADGKGRVAALEIMLGTPAIANLIREGKTFQIPSIIQTAKRDGMQLMDQH
LLDLFKTKRITAEEAYRCAQDKKQFEQYLTEKPGQ

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=312, Percent_Identity=45.1923076923077, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1789723, Length=289, Percent_Identity=33.2179930795848, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786296, Length=190, Percent_Identity=41.5789473684211, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39671; Mature: 39540

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIDALFKLLKEQGASDLHLSSGAPPIFRLHGEMARQNFKVLSHEELTAILYEILTDKQ
CCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
KADFEERRDLDFAYAIPGLARFRGNYMMTHRGIAAVFRIIPSKILSADDLSLPDGVRRMT
HCCHHHHHCCCHHHHCCHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
QFKKGLVLVTGPTGSGKSTTLAAMIDLINATRKEHILTLEDPLEFIHENKMSLLNQRQIG
HHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHHCC
EHSLSFSAALRAALREDPDVILVGEMRDLETIGLAMSAAETGHLVFGTLHTNSAAKTIDR
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHH
IIDVFPTDQQEQTRAMLSESLKGVVCQQLLKTADGKGRVAALEIMLGTPAIANLIREGKT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHCCCC
FQIPSIIQTAKRDGMQLMDQHLLDLFKTKRITAEEAYRCAQDKKQFEQYLTEKPGQ
EECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ARIDALFKLLKEQGASDLHLSSGAPPIFRLHGEMARQNFKVLSHEELTAILYEILTDKQ
CHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
KADFEERRDLDFAYAIPGLARFRGNYMMTHRGIAAVFRIIPSKILSADDLSLPDGVRRMT
HCCHHHHHCCCHHHHCCHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
QFKKGLVLVTGPTGSGKSTTLAAMIDLINATRKEHILTLEDPLEFIHENKMSLLNQRQIG
HHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHHCC
EHSLSFSAALRAALREDPDVILVGEMRDLETIGLAMSAAETGHLVFGTLHTNSAAKTIDR
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHH
IIDVFPTDQQEQTRAMLSESLKGVVCQQLLKTADGKGRVAALEIMLGTPAIANLIREGKT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHCCCC
FQIPSIIQTAKRDGMQLMDQHLLDLFKTKRITAEEAYRCAQDKKQFEQYLTEKPGQ
EECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]