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The map label for this gene is epsE [H]

Identifier: 39995543

GI number: 39995543

Start: 466122

End: 467771

Strand: Direct

Name: epsE [H]

Synonym: GSU0435

Alternate gene names: 39995543

Gene position: 466122-467771 (Clockwise)

Preceding gene: 39995541

Following gene: 39995544

Centisome position: 12.22

GC content: 65.76

Gene sequence:

>1650_bases
ATGGAAAGCATCGTCAAGGAAGGGTCTCTCGGGTCCATTCTCTTCAAATGCCAGATCATCAGCGAGGACGACATCCGGCG
GGCACTCGATGAGCAGGAGCGCACCGGAGGCCGCTTCGGCGAGGCACTGGTATCACTCGGCATTGTAACCCAGGAAGACA
TCGACTGGGCCCTGTCGAACCAGCTCAACATCCCCTACGTACGCCTCAAGCCGGCCATGGTCGACCGGGATGCCGTGGCG
TTGGTCCCGGCGGTCATGGCCCGACAGCATAATCTCATCCCCCTGATCAGGGCCGGCGAGGAACTGAGCATCGCCATTGC
CGACCCGCTCAACGTGGCGGCAGTGGCGGCCGTGGAAAAGGAGACCGGGTGCGCCGTGTCGGTTTCCGTGGCGCTCATCC
GGGAGATCCGGGAGATGCAGGAGCGTTTCTACGGACCGCCCGACACGGAGGAACGCCTGGGGTTCACGTCGTCGGCGTTC
CCACCCCAGGCCCTTGCCGCCATGAATCACGACCTGACAGGGGGGAAGTTCATCGATTACCTGTTGCTGTTCGTGGCCCA
GCAAAAGCTCAGCTCCCTTTCGCTCCACCCCCTGGGGGACAGGGTGTCGGTGATTGGCCGGCGCGGCGGCACCACGCGGG
AGGTCGGACAGCTTGCCCCTTCCCGCTATCCCGACGTGGTCATGCACGTCAAGAAACTTGCCCACATCGACGGGGCCCGG
TTCTCCGCCCGTGGGGGGCTATCCTTTGCCCTGAAGGGCCGCTCCATTCCCTTTCAGGTGGCTACCCTGCGGGGAGAAGG
CGGCGATCACCTCACCTTCAGGATGACGGTGGCGGCATTGTTCCCGACATCCCTTGCCGACCTGGGGCTGACGGACGACC
AAGTCCGGCAGTTTGCCGATCTGGCGGCGGCCGGTCGCGGCATGGTGGTGACCGGAGCCCGGGATCGGGAGATTCGCCGC
CGGCTCACGGATCTCTACCTTCAGGAGCATGAGGCGGAGGGGAAGACCGTGCTGGTCGTCGGCAGCGGCGCCGGCACGGG
AGAGCAGCGGTTCCCCCGTATTCCGGTGCCGTCCGACGCGGATCTGAGCGCTGTGGTTTCAGCCTGTCTGGAGCATGATC
CGGATATTCTCGTCCTGGAGGATGTCACTGACGGCCAGGCCTTTGCGGCAGCATGCCGGGCAACTCTGCGCGGTAAGCTG
GTGGTGGCGGGGATCGGCTGCGGTGACGCCGTCGGCGCCCTGGACCAGCTTATCGCCTTCCGGGACATGCACGTTCTCGT
GCCTGCGTATCTGCGGGGGGTGATTACCTGCACGCCGATCCGGCCCCTGTGCCCCGCATGCCGGCGAAGCGAGCCATTCC
CCGCCGCAGAGCGGGCGGCTCTGGGGATCGGTGCCGACGTCACCTCTTGCTGGCGGTCGGCGGGATGCGAATCCTGCGAC
CAGACCGGCCATGACGGCAGGCGCTACCTGCTGGATGTGCTGGTTTTGGACCACGACCTCCGGGAGCGGTTCGAGGCGGC
CCGCAACGGGGCAGAGGTGATCGAGCATTTGCGCGGACAGGGTTGGCGCGGGATCACGGACGAGCGGCAGACCCTGCTGG
CTGAAGGCACTATTTCGCTGGAGGAGTACGCCTCCTCCCTGCACGGTTGA

Upstream 100 bases:

>100_bases
TAGCACGGGGGGGCGAAAAAAGTAAAGGGTGCTCGCGCCTGAAAATAACCCTCAAGTCACGGCAGGTTCCGGCCGATTGG
ATGTAGAGGGAGGAGCACCC

Downstream 100 bases:

>100_bases
CGGAGACGGTTTCATGGCACGTATCGACGCACTGTTCAAGCTGCTGAAGGAGCAAGGGGCCTCCGACCTCCACCTGTCGT
CCGGGGCTCCGCCCATTTTC

Product: MSHA biogenesis protein MshE

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MESIVKEGSLGSILFKCQIISEDDIRRALDEQERTGGRFGEALVSLGIVTQEDIDWALSNQLNIPYVRLKPAMVDRDAVA
LVPAVMARQHNLIPLIRAGEELSIAIADPLNVAAVAAVEKETGCAVSVSVALIREIREMQERFYGPPDTEERLGFTSSAF
PPQALAAMNHDLTGGKFIDYLLLFVAQQKLSSLSLHPLGDRVSVIGRRGGTTREVGQLAPSRYPDVVMHVKKLAHIDGAR
FSARGGLSFALKGRSIPFQVATLRGEGGDHLTFRMTVAALFPTSLADLGLTDDQVRQFADLAAAGRGMVVTGARDREIRR
RLTDLYLQEHEAEGKTVLVVGSGAGTGEQRFPRIPVPSDADLSAVVSACLEHDPDILVLEDVTDGQAFAAACRATLRGKL
VVAGIGCGDAVGALDQLIAFRDMHVLVPAYLRGVITCTPIRPLCPACRRSEPFPAAERAALGIGADVTSCWRSAGCESCD
QTGHDGRRYLLDVLVLDHDLRERFEAARNGAEVIEHLRGQGWRGITDERQTLLAEGTISLEEYASSLHG

Sequences:

>Translated_549_residues
MESIVKEGSLGSILFKCQIISEDDIRRALDEQERTGGRFGEALVSLGIVTQEDIDWALSNQLNIPYVRLKPAMVDRDAVA
LVPAVMARQHNLIPLIRAGEELSIAIADPLNVAAVAAVEKETGCAVSVSVALIREIREMQERFYGPPDTEERLGFTSSAF
PPQALAAMNHDLTGGKFIDYLLLFVAQQKLSSLSLHPLGDRVSVIGRRGGTTREVGQLAPSRYPDVVMHVKKLAHIDGAR
FSARGGLSFALKGRSIPFQVATLRGEGGDHLTFRMTVAALFPTSLADLGLTDDQVRQFADLAAAGRGMVVTGARDREIRR
RLTDLYLQEHEAEGKTVLVVGSGAGTGEQRFPRIPVPSDADLSAVVSACLEHDPDILVLEDVTDGQAFAAACRATLRGKL
VVAGIGCGDAVGALDQLIAFRDMHVLVPAYLRGVITCTPIRPLCPACRRSEPFPAAERAALGIGADVTSCWRSAGCESCD
QTGHDGRRYLLDVLVLDHDLRERFEAARNGAEVIEHLRGQGWRGITDERQTLLAEGTISLEEYASSLHG
>Mature_549_residues
MESIVKEGSLGSILFKCQIISEDDIRRALDEQERTGGRFGEALVSLGIVTQEDIDWALSNQLNIPYVRLKPAMVDRDAVA
LVPAVMARQHNLIPLIRAGEELSIAIADPLNVAAVAAVEKETGCAVSVSVALIREIREMQERFYGPPDTEERLGFTSSAF
PPQALAAMNHDLTGGKFIDYLLLFVAQQKLSSLSLHPLGDRVSVIGRRGGTTREVGQLAPSRYPDVVMHVKKLAHIDGAR
FSARGGLSFALKGRSIPFQVATLRGEGGDHLTFRMTVAALFPTSLADLGLTDDQVRQFADLAAAGRGMVVTGARDREIRR
RLTDLYLQEHEAEGKTVLVVGSGAGTGEQRFPRIPVPSDADLSAVVSACLEHDPDILVLEDVTDGQAFAAACRATLRGKL
VVAGIGCGDAVGALDQLIAFRDMHVLVPAYLRGVITCTPIRPLCPACRRSEPFPAAERAALGIGADVTSCWRSAGCESCD
QTGHDGRRYLLDVLVLDHDLRERFEAARNGAEVIEHLRGQGWRGITDERQTLLAEGTISLEEYASSLHG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=337, Percent_Identity=25.2225519287834, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1786296, Length=367, Percent_Identity=23.433242506812, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 59283; Mature: 59283

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIVKEGSLGSILFKCQIISEDDIRRALDEQERTGGRFGEALVSLGIVTQEDIDWALSN
CCCHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCEEHHCCCHHHCC
QLNIPYVRLKPAMVDRDAVALVPAVMARQHNLIPLIRAGEELSIAIADPLNVAAVAAVEK
CCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCHHHEEECC
ETGCAVSVSVALIREIREMQERFYGPPDTEERLGFTSSAFPPQALAAMNHDLTGGKFIDY
CCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHH
LLLFVAQQKLSSLSLHPLGDRVSVIGRRGGTTREVGQLAPSRYPDVVMHVKKLAHIDGAR
HHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
FSARGGLSFALKGRSIPFQVATLRGEGGDHLTFRMTVAALFPTSLADLGLTDDQVRQFAD
EECCCCCEEEEECCCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHH
LAAAGRGMVVTGARDREIRRRLTDLYLQEHEAEGKTVLVVGSGAGTGEQRFPRIPVPSDA
HHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
DLSAVVSACLEHDPDILVLEDVTDGQAFAAACRATLRGKLVVAGIGCGDAVGALDQLIAF
CHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
RDMHVLVPAYLRGVITCTPIRPLCPACRRSEPFPAAERAALGIGADVTSCWRSAGCESCD
HHHHHHHHHHHCCCEEECCCCHHCHHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHH
QTGHDGRRYLLDVLVLDHDLRERFEAARNGAEVIEHLRGQGWRGITDERQTLLAEGTISL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCH
EEYASSLHG
HHHHHHHCC
>Mature Secondary Structure
MESIVKEGSLGSILFKCQIISEDDIRRALDEQERTGGRFGEALVSLGIVTQEDIDWALSN
CCCHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCEEHHCCCHHHCC
QLNIPYVRLKPAMVDRDAVALVPAVMARQHNLIPLIRAGEELSIAIADPLNVAAVAAVEK
CCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCHHHEEECC
ETGCAVSVSVALIREIREMQERFYGPPDTEERLGFTSSAFPPQALAAMNHDLTGGKFIDY
CCCCEEHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHH
LLLFVAQQKLSSLSLHPLGDRVSVIGRRGGTTREVGQLAPSRYPDVVMHVKKLAHIDGAR
HHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
FSARGGLSFALKGRSIPFQVATLRGEGGDHLTFRMTVAALFPTSLADLGLTDDQVRQFAD
EECCCCCEEEEECCCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHH
LAAAGRGMVVTGARDREIRRRLTDLYLQEHEAEGKTVLVVGSGAGTGEQRFPRIPVPSDA
HHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
DLSAVVSACLEHDPDILVLEDVTDGQAFAAACRATLRGKLVVAGIGCGDAVGALDQLIAF
CHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
RDMHVLVPAYLRGVITCTPIRPLCPACRRSEPFPAAERAALGIGADVTSCWRSAGCESCD
HHHHHHHHHHHCCCEEECCCCHHCHHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHH
QTGHDGRRYLLDVLVLDHDLRERFEAARNGAEVIEHLRGQGWRGITDERQTLLAEGTISL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCH
EEYASSLHG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]