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The map label for this gene is pit [H]

Identifier: 39995497

GI number: 39995497

Start: 420726

End: 421727

Strand: Direct

Name: pit [H]

Synonym: GSU0389

Alternate gene names: 39995497

Gene position: 420726-421727 (Clockwise)

Preceding gene: 39995496

Following gene: 39995499

Centisome position: 11.03

GC content: 60.48

Gene sequence:

>1002_bases
ATGCTTGACACTACGCTGATCATGCTCGTCCTGGTTATCGGGGCGGCACTTGCGTTCGACTACATCAACGGATTTCACGA
CACGGCCAATGCGATTGCCACCTGCGTTTCGACTCGGGCACTGTCGGTCCGGTCGGCCATTGTCATGGCGGCAGTGCTCA
ACTTTGCGGGAGCAATGATTTCGACCAAGGTGGCCGCCACGATCGGCAAGGGGATCGTGGACGCCGGCAACGTGACGCAG
ATGGTGGTGCTCGCCGGGGTCTTGGGTGCGATTATCTGGGACCTCGTCACGTGGTACTACGGGCTTCCCTCTTCGTCGTC
ACACGCCATCATCGGCGGAATCATGGGCGCGGTCATCGCCCACGCCGGCACGACGGCCCTCCATTGGGGCGGGCTGAAAA
AGATCATCCTCTCGCTCATCCTTTCTCCTGTCATTGGAACGCTCCTCGGGTTCATCGTCATGGTGGTCATGCTCTGGAGC
TTCAAGAGCACCTCGCCCCACTCCATAAACAAGCACTTCCGTCGTCTGCAAGTCCTTTCGGCCGCATTCATGGCCTTTTC
CCACGGCACCGCCGACGCCCAGAAGTCCATGGGCGTCATCACCATGGCGCTGGTGAGTTATGGAACCCTCACCACCTTCG
ATGTTCCAACCTGGGTCAAGATTGCCTGTGCCGTGGCCATGGGCCTCGGCACCGCGGCCGGCGGCTGGCGGATCATCAAG
ACCGTGGGGAAGGATTTCGTCAAGCTGCAGCCGGTCCATGGCTTCTGCGTCGAAACCGCTTCGGCCGGCGTCATCCTGGG
GGCGTCCTCCATCGGCATGCCGGTCAGCACTACCCACGTCATCACCTCCACTATCCTCGGCGTGGGGCTCTCCAAGCGGA
TCACGGCCGTGAACTGGAACGTGGCCTACCGTATCCTCTGGGCCTGGGTCCTGACCATCCCCGCTTCGGCGATCATGTCC
TATGTTTCATACATGGTTTTGAACCCGTTCCTCGGCAAATAA

Upstream 100 bases:

>100_bases
ATCCAGCTCATCAAGTGGAAGGAGATCTACGAGACCCTGGAGCGGGCCACGGACAAATGCGAAGATGCCGCCAACATCCT
CGAAAGCGTGGTGGTGAAGA

Downstream 100 bases:

>100_bases
AAAGGGGGCGACCGGATGGTCGCCCCTTCTGCATTCCGCTGTCCCCGGTTTATTACTCCAGTTCTTCCCCCACCTCTTCC
ATTTCCTCCGCCAGGGTCGC

Product: phosphate transport system regulatory protein PhoU

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MLDTTLIMLVLVIGAALAFDYINGFHDTANAIATCVSTRALSVRSAIVMAAVLNFAGAMISTKVAATIGKGIVDAGNVTQ
MVVLAGVLGAIIWDLVTWYYGLPSSSSHAIIGGIMGAVIAHAGTTALHWGGLKKIILSLILSPVIGTLLGFIVMVVMLWS
FKSTSPHSINKHFRRLQVLSAAFMAFSHGTADAQKSMGVITMALVSYGTLTTFDVPTWVKIACAVAMGLGTAAGGWRIIK
TVGKDFVKLQPVHGFCVETASAGVILGASSIGMPVSTTHVITSTILGVGLSKRITAVNWNVAYRILWAWVLTIPASAIMS
YVSYMVLNPFLGK

Sequences:

>Translated_333_residues
MLDTTLIMLVLVIGAALAFDYINGFHDTANAIATCVSTRALSVRSAIVMAAVLNFAGAMISTKVAATIGKGIVDAGNVTQ
MVVLAGVLGAIIWDLVTWYYGLPSSSSHAIIGGIMGAVIAHAGTTALHWGGLKKIILSLILSPVIGTLLGFIVMVVMLWS
FKSTSPHSINKHFRRLQVLSAAFMAFSHGTADAQKSMGVITMALVSYGTLTTFDVPTWVKIACAVAMGLGTAAGGWRIIK
TVGKDFVKLQPVHGFCVETASAGVILGASSIGMPVSTTHVITSTILGVGLSKRITAVNWNVAYRILWAWVLTIPASAIMS
YVSYMVLNPFLGK
>Mature_333_residues
MLDTTLIMLVLVIGAALAFDYINGFHDTANAIATCVSTRALSVRSAIVMAAVLNFAGAMISTKVAATIGKGIVDAGNVTQ
MVVLAGVLGAIIWDLVTWYYGLPSSSSHAIIGGIMGAVIAHAGTTALHWGGLKKIILSLILSPVIGTLLGFIVMVVMLWS
FKSTSPHSINKHFRRLQVLSAAFMAFSHGTADAQKSMGVITMALVSYGTLTTFDVPTWVKIACAVAMGLGTAAGGWRIIK
TVGKDFVKLQPVHGFCVETASAGVILGASSIGMPVSTTHVITSTILGVGLSKRITAVNWNVAYRILWAWVLTIPASAIMS
YVSYMVLNPFLGK

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=164, Percent_Identity=34.1463414634146, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI5803173, Length=151, Percent_Identity=35.0993377483444, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1789360, Length=217, Percent_Identity=36.8663594470046, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=36.8663594470046, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17539280, Length=175, Percent_Identity=30.2857142857143, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71981576, Length=157, Percent_Identity=31.8471337579618, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25146401, Length=160, Percent_Identity=30.625, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566716, Length=153, Percent_Identity=28.1045751633987, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17536725, Length=181, Percent_Identity=28.1767955801105, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17557328, Length=170, Percent_Identity=27.6470588235294, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319773, Length=163, Percent_Identity=33.7423312883436, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356511, Length=176, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 34958; Mature: 34958

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLDTTLIMLVLVIGAALAFDYINGFHDTANAIATCVSTRALSVRSAIVMAAVLNFAGAMI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STKVAATIGKGIVDAGNVTQMVVLAGVLGAIIWDLVTWYYGLPSSSSHAIIGGIMGAVIA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
HAGTTALHWGGLKKIILSLILSPVIGTLLGFIVMVVMLWSFKSTSPHSINKHFRRLQVLS
HCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AAFMAFSHGTADAQKSMGVITMALVSYGTLTTFDVPTWVKIACAVAMGLGTAAGGWRIIK
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCCCCHHHHH
TVGKDFVKLQPVHGFCVETASAGVILGASSIGMPVSTTHVITSTILGVGLSKRITAVNWN
HHHHHHEEECCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCHHHCEEEEEHH
VAYRILWAWVLTIPASAIMSYVSYMVLNPFLGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]