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The map label for this gene is fmt

Identifier: 39995241

GI number: 39995241

Start: 144850

End: 145803

Strand: Direct

Name: fmt

Synonym: GSU0130

Alternate gene names: 39995241

Gene position: 144850-145803 (Clockwise)

Preceding gene: 39995240

Following gene: 39995242

Centisome position: 3.8

GC content: 64.47

Gene sequence:

>954_bases
ATGGCCGGACTGCGCATCATCTTCATGGGAACCCCCGAATTTGCCTGCCCCACGCTCAGGAAGCTGATCGAGCGCGGCGA
GGAGGTCATCGCCGTCGTCACCCAGCCCGACCGTCCCAAGGGGCGCGGCCAGAAGCTCGTGCCGCCACCGGTGAAGGCCC
TGGCCCAAGAGCACGACATCCCGGTGCTCCAGCCCCTCAAGGTGCGAACCCCGGAGTCCGTCGATGAGATTCGCCGGCTC
GCCCCCGATCTCATCGTCGTCGTGGCCTTCGGCCAGATTCTGCCCCAGAGCCTGCTCGATATCCCGAAGCACGGCTGTAT
CAACATTCATGCCTCGCTCCTTCCCCGCTACCGGGGAGCGGCTCCTCTCAACTGGTGTCTCATCAACGGCGAGACCGAGA
CCGGAATCACTACCATGATGATGGATGCGGGCCTCGACACCGGGGACATGCTCGTGAAACGGGCCATCCCCATCGGGCCC
GACGAGGATGCCCAGTCCCTCCATGACCGCCTGTCGCAATTGGGTGCCGAGACCATCGACGAGACCCTCGATCTCTTGCT
GGCCGGCAAACTGGTGCGCGAAAAGCAGGATGATTCCCTCACCTGCTACGCTCCCATGCTCAAGAAGGAGGATGGCCTCG
TCGACTGGACCCGAGAGCCGGTGCAAGTCAAGAACCAGGTGCGGGGCTTTACCCCGTGGCCGGGAGCCTATACCTTCCTG
GACGGGAAGACCCTCAAGCTCTACCGGGTGGCTGTCGCCGGTGAAACCGGCGAGCCCGGCGAGATCCTGAGGGTTGGCAG
GGAGGGGATTCTCGTGGGATGCGGCTCCGGCAGCATTCTGATCCAGGAGTTGCAGCTGGAGGGACGCAAGCGGCTCCCGA
CTGCCGAGTTTCTTGCCGGATTCCGCCTGGAACCGGGAACGCGCCTCGGTGAGGCAGGGAGCGTGGAGCACTGA

Upstream 100 bases:

>100_bases
CTGGACGGCGTCCTTTTTGTGGATCACCTCTCCGCGCTCAAGAAGGAAATGTTCAAGAAAAAATACCGCCGCATGACCGA
AGAGGAGTGAGGATCAACCT

Downstream 100 bases:

>100_bases
CAATGGCGGAACACCCTCCCCAGAAACGTCTGTTCATCGCCCTGATGGGGCTTACCTGCCTCCTGATCGTGGGGGGAATC
TACCTTCTCTGGTGGGTACC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MAGLRIIFMGTPEFACPTLRKLIERGEEVIAVVTQPDRPKGRGQKLVPPPVKALAQEHDIPVLQPLKVRTPESVDEIRRL
APDLIVVVAFGQILPQSLLDIPKHGCINIHASLLPRYRGAAPLNWCLINGETETGITTMMMDAGLDTGDMLVKRAIPIGP
DEDAQSLHDRLSQLGAETIDETLDLLLAGKLVREKQDDSLTCYAPMLKKEDGLVDWTREPVQVKNQVRGFTPWPGAYTFL
DGKTLKLYRVAVAGETGEPGEILRVGREGILVGCGSGSILIQELQLEGRKRLPTAEFLAGFRLEPGTRLGEAGSVEH

Sequences:

>Translated_317_residues
MAGLRIIFMGTPEFACPTLRKLIERGEEVIAVVTQPDRPKGRGQKLVPPPVKALAQEHDIPVLQPLKVRTPESVDEIRRL
APDLIVVVAFGQILPQSLLDIPKHGCINIHASLLPRYRGAAPLNWCLINGETETGITTMMMDAGLDTGDMLVKRAIPIGP
DEDAQSLHDRLSQLGAETIDETLDLLLAGKLVREKQDDSLTCYAPMLKKEDGLVDWTREPVQVKNQVRGFTPWPGAYTFL
DGKTLKLYRVAVAGETGEPGEILRVGREGILVGCGSGSILIQELQLEGRKRLPTAEFLAGFRLEPGTRLGEAGSVEH
>Mature_316_residues
AGLRIIFMGTPEFACPTLRKLIERGEEVIAVVTQPDRPKGRGQKLVPPPVKALAQEHDIPVLQPLKVRTPESVDEIRRLA
PDLIVVVAFGQILPQSLLDIPKHGCINIHASLLPRYRGAAPLNWCLINGETETGITTMMMDAGLDTGDMLVKRAIPIGPD
EDAQSLHDRLSQLGAETIDETLDLLLAGKLVREKQDDSLTCYAPMLKKEDGLVDWTREPVQVKNQVRGFTPWPGAYTFLD
GKTLKLYRVAVAGETGEPGEILRVGREGILVGCGSGSILIQELQLEGRKRLPTAEFLAGFRLEPGTRLGEAGSVEH

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=313, Percent_Identity=29.7124600638978, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI21614513, Length=316, Percent_Identity=27.8481012658228, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI164663775, Length=319, Percent_Identity=28.2131661442006, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1789683, Length=311, Percent_Identity=43.0868167202572, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1788589, Length=282, Percent_Identity=31.2056737588652, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI133930964, Length=259, Percent_Identity=27.7992277992278, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319458, Length=263, Percent_Identity=25.8555133079848, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550868, Length=305, Percent_Identity=30.4918032786885, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28571984, Length=227, Percent_Identity=33.0396475770925, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24585660, Length=241, Percent_Identity=27.8008298755187, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24582400, Length=107, Percent_Identity=35.5140186915888, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_GEOSL (Q74GW4)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951192.1
- ProteinModelPortal:   Q74GW4
- SMR:   Q74GW4
- GeneID:   2687945
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0130
- NMPDR:   fig|243231.1.peg.130
- TIGR:   GSU0130
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   CLSK827657
- BioCyc:   GSUL243231:GSU_0130-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34669; Mature: 34538

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00373 GART; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLRIIFMGTPEFACPTLRKLIERGEEVIAVVTQPDRPKGRGQKLVPPPVKALAQEHDI
CCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCC
PVLQPLKVRTPESVDEIRRLAPDLIVVVAFGQILPQSLLDIPKHGCINIHASLLPRYRGA
CCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHCCC
APLNWCLINGETETGITTMMMDAGLDTGDMLVKRAIPIGPDEDAQSLHDRLSQLGAETID
CCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ETLDLLLAGKLVREKQDDSLTCYAPMLKKEDGLVDWTREPVQVKNQVRGFTPWPGAYTFL
HHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEE
DGKTLKLYRVAVAGETGEPGEILRVGREGILVGCGSGSILIQELQLEGRKRLPTAEFLAG
CCCEEEEEEEEEECCCCCCHHHHEECCCEEEEECCCCCEEEEEEHHCCCCCCCHHHHHHC
FRLEPGTRLGEAGSVEH
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGLRIIFMGTPEFACPTLRKLIERGEEVIAVVTQPDRPKGRGQKLVPPPVKALAQEHDI
CCEEEEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCC
PVLQPLKVRTPESVDEIRRLAPDLIVVVAFGQILPQSLLDIPKHGCINIHASLLPRYRGA
CCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHCCC
APLNWCLINGETETGITTMMMDAGLDTGDMLVKRAIPIGPDEDAQSLHDRLSQLGAETID
CCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ETLDLLLAGKLVREKQDDSLTCYAPMLKKEDGLVDWTREPVQVKNQVRGFTPWPGAYTFL
HHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEE
DGKTLKLYRVAVAGETGEPGEILRVGREGILVGCGSGSILIQELQLEGRKRLPTAEFLAG
CCCEEEEEEEEEECCCCCCHHHHEECCCEEEEECCCCCEEEEEEHHCCCCCCCHHHHHHC
FRLEPGTRLGEAGSVEH
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA