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The map label for this gene is def-1 [H]

Identifier: 39995240

GI number: 39995240

Start: 144291

End: 144839

Strand: Direct

Name: def-1 [H]

Synonym: GSU0129

Alternate gene names: 39995240

Gene position: 144291-144839 (Clockwise)

Preceding gene: 39995239

Following gene: 39995241

Centisome position: 3.78

GC content: 57.92

Gene sequence:

>549_bases
TTGGCTATTATCAAAAAGATTAACCCATTACGGAAGGTTCAGACCATGGTACGCACGATCCTCACCTATCCCGATCCGGT
GCTGAAGAAAAAAGCGGTGCCGGTTACCATCATCAACGACGCCACCCGTGAACTGGTTCGCGACATGGCCGAAACCATGT
ACGATGCCCAGGGCGTCGGGCTGGCGGCCCCCCAGATCGGCGTGAGCCAGCGGGTCATCGTCATTGATGTTTCACAGCGC
GACGAGCGGCCCGAGCTGATCGTCTGCATCAACCCGGTCATCATTCATGGCGAGGGAGAGTCCTACGAGGAGGAGGGGTG
CCTTTCGGTCCCCAAGTACTCGGCCAATGTCCACCGCCACGAGCGGGTGGTGGTCAAGTCCCTCAACCTGGAGGGAGAGG
AGGTCGTCCATCGGGCGGAGGGACTGCTTGCCATCGCCTTTCAGCATGAGATCGACCACCTGGACGGCGTCCTTTTTGTG
GATCACCTCTCCGCGCTCAAGAAGGAAATGTTCAAGAAAAAATACCGCCGCATGACCGAAGAGGAGTGA

Upstream 100 bases:

>100_bases
CCAGCCTCAGGCTGGCGTGAGGCTCCTGGTTGACATCGACCCCGTGGATCTCATGTAAACCCCCATGCCCCTCGTTTAGA
TTTGATTTTTCCCGCCGCAA

Downstream 100 bases:

>100_bases
GGATCAACCTATGGCCGGACTGCGCATCATCTTCATGGGAACCCCCGAATTTGCCTGCCCCACGCTCAGGAAGCTGATCG
AGCGCGGCGAGGAGGTCATC

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MAIIKKINPLRKVQTMVRTILTYPDPVLKKKAVPVTIINDATRELVRDMAETMYDAQGVGLAAPQIGVSQRVIVIDVSQR
DERPELIVCINPVIIHGEGESYEEEGCLSVPKYSANVHRHERVVVKSLNLEGEEVVHRAEGLLAIAFQHEIDHLDGVLFV
DHLSALKKEMFKKKYRRMTEEE

Sequences:

>Translated_182_residues
MAIIKKINPLRKVQTMVRTILTYPDPVLKKKAVPVTIINDATRELVRDMAETMYDAQGVGLAAPQIGVSQRVIVIDVSQR
DERPELIVCINPVIIHGEGESYEEEGCLSVPKYSANVHRHERVVVKSLNLEGEEVVHRAEGLLAIAFQHEIDHLDGVLFV
DHLSALKKEMFKKKYRRMTEEE
>Mature_181_residues
AIIKKINPLRKVQTMVRTILTYPDPVLKKKAVPVTIINDATRELVRDMAETMYDAQGVGLAAPQIGVSQRVIVIDVSQRD
ERPELIVCINPVIIHGEGESYEEEGCLSVPKYSANVHRHERVVVKSLNLEGEEVVHRAEGLLAIAFQHEIDHLDGVLFVD
HLSALKKEMFKKKYRRMTEEE

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=160, Percent_Identity=33.125, Blast_Score=74, Evalue=6e-14,
Organism=Escherichia coli, GI1789682, Length=163, Percent_Identity=46.6257668711656, Blast_Score=139, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=36.3057324840764, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=31.8471337579618, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20634; Mature: 20503

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIIKKINPLRKVQTMVRTILTYPDPVLKKKAVPVTIINDATRELVRDMAETMYDAQGVG
CCCHHHCCHHHHHHHHHHHHHCCCCHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCC
LAAPQIGVSQRVIVIDVSQRDERPELIVCINPVIIHGEGESYEEEGCLSVPKYSANVHRH
CCCCCCCCCCEEEEEECCCCCCCCCEEEEECEEEEECCCCCCCCCCCCCCCCCCCCHHHH
ERVVVKSLNLEGEEVVHRAEGLLAIAFQHEIDHLDGVLFVDHLSALKKEMFKKKYRRMTE
HHHHHHHCCCCHHHHHHHHCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
EE
CC
>Mature Secondary Structure 
AIIKKINPLRKVQTMVRTILTYPDPVLKKKAVPVTIINDATRELVRDMAETMYDAQGVG
CCHHHCCHHHHHHHHHHHHHCCCCHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCC
LAAPQIGVSQRVIVIDVSQRDERPELIVCINPVIIHGEGESYEEEGCLSVPKYSANVHRH
CCCCCCCCCCEEEEEECCCCCCCCCEEEEECEEEEECCCCCCCCCCCCCCCCCCCCHHHH
ERVVVKSLNLEGEEVVHRAEGLLAIAFQHEIDHLDGVLFVDHLSALKKEMFKKKYRRMTE
HHHHHHHCCCCHHHHHHHHCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA