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The map label for this gene is priA [H]

Identifier: 39995239

GI number: 39995239

Start: 141993

End: 144248

Strand: Direct

Name: priA [H]

Synonym: GSU0128

Alternate gene names: 39995239

Gene position: 141993-144248 (Clockwise)

Preceding gene: 39995227

Following gene: 39995240

Centisome position: 3.72

GC content: 67.38

Gene sequence:

>2256_bases
ATGTCATCATTCCCCGTGAACGAACGGCAGACCGTCATTGTCCACGTGGCGGTTCCGCTTCCGCTCGATGCCCCCTTTTC
CTACCGCGTGCCCGCTCCGCTGGCTCCGGCGGCTGCGGTGGGGCGGCGGCTCCTCGTCCCCTTCGGCCGACGGAAAGTGA
CCGGCTACGTGCTGGAGGTTGCCGATGACGACGGACGGGAGCTGCGCGACGTTCTTGAGTTCCTGGACGATGGCCCCCTC
TTCACACCCGGCGAACTGGCTCTGTACCGCTGGGCTTCTTCCTATTATCTCCACCCCCTCGGCGAGGTGATCAAGGCGGC
GCTCCCGGCGGGGATCAACGTCCAGAGTCGCCGCGGCGCGGGCCTGGACGAGGGAGAGCTCACCGGTGGCCGCTCGGTGA
AGACCGAACGGGTCTATCGCGCCGTTGCCGAGGTGCCGGATACTGTCCCTCCGCCGGGCAAGGCCTCGGAGCTGCTGGCG
CACCTGGCGGAGCGGGAGGAGGAATCGGCGTCGGAGCTGCGGCGTCTCTTCGGTGAATGTTCAGTTCAGCTCAGGCGGCT
GCGGGAGCTGGGATTCGTGGAGATGGCTGAGCGGGAAATCTACCGGGACCCGTTCCGGATGGAAGCCGTTGGTCACGACC
GTCCCCTTGAGCTCAACGAGCGGCAGTCCGCTGCCCTGGTCCGGCTGCGGGCGGCCCTGGACGCTGGACGGTTCACCCCT
TTCCTGCTCCGGGGCGTCACCGGCAGCGGCAAGACGGAAGTCTATCTCCAGGCCATCGCCCATTGTCTCGAACAGGGAAG
GAGCGCCTTGGTCCTGGTCCCCGAGATCGCCCTCACTCCCCAGCTGGTAAGCCGGTTCCGCCGGCGGTTCCGGTGCGGGG
TGGCAGTGCTCCACAGCGGTCTCTCCGATGGAGAGCGCTTCGATGAATGGCGCCGGATCCGCCGGGGTGAGGCGGCCATC
GTCATCGGTGCCCGCTCGGCGATTTTCGCTCCTCTGGCCGATCTGGGCATGATCGTGGTGGACGAGGAACACGAAGGTTC
TTACAAGCAGGGGGAGGGGTTCCGCTACAACGCCCGCGACATGGCCCTGGTACGGGGCAAGATGGCCGGGGCTTGCGTGG
TCCTCGGCACCGCTACGCCCCAGGTCACTACCTGGTATGCGGTTGCCGGCGGCAAGCTCGAATGCCTCGATCTCCCCGAA
CGGGTGAACGGCCTTCCCCTGCCGTCGGCAGAGATCATCGACGTCCGGGGTGCCAGGGGACATGTCCTCCATCCACGCCT
CGCCGCGGAGGTGGAGGCGAACCTGGCGCGCGGCGAGCAAACCCTCCTCTTCCTCAACCGCCGCGGCTTCTCCACCTGGC
TCACCTGCGAAGAATGCGGCCACGTGCTCCGCTGCCCGAATTGCCAGGTAACCCTCACCTACCACCAGGGCAGGAACCGG
CACATCTGCCACTACTGCGATTATGCCGTTCCGGCCCCGTCCATGTGTCCGGGGTGCGGCTGCGGACGGATCATCCACCT
GGGGCTCGGCACCGAACAGGTGGAAGCGTACGTGCGGGAGCACTTTCCCCATGCCCGGGTGGACCGGATGGACCGGGACA
CCACTGCCCGCAGGGGGGGGCACGCCCGGATCCTGCGCAAGGTGGCGGAGCGACAGACCGACATCCTCATCGGCACCCAG
ATGGTGGCCAAAGGGCATGACTTCCCGGGGATCACCCTGGTGGGGATACTGTCGGTGGAATCGACCCTCAATATCCCCGA
TTACCGGAGTGCCGAGCGGACGTTCCAGCTGGTGACCCAACTCATCGGACGGGCAGGACGGGGGGATGATCCGGGCCGGG
TGCTGATCCAGAGCCTCAATCCCGATCACTACGCCCTGGCCCACGCCGTGGCCGGCGATATGGACGGTTTTTACGGGGCA
GAACTGGCGTTCCGCCAGGAGACCGGCTACCCCCCCTTTGCCCATCTGGCGGCCCTCTGTCTGAGCGGCACCGCCGCATC
GGCCGTGGAGCGGACAAGTGAGCTCCTGGCGGCGCGGCTGCGCGAATTGCGCCGGGATGGGGGCGGGCGGGTCGAGATTC
TGGGGCCCGCGCCCGCGCCCCTGGCCAAGCTTCGCGGCCGTTTCCGCCGGCAGATCCTTCTGAAGGCCGCAGGCCGGACT
GCCCTCCATCGCCTCGTCTGTCGGGCGCGCGAGAGCTTCCAGCCTCAGGCTGGCGTGAGGCTCCTGGTTGACATCGACCC
CGTGGATCTCATGTAA

Upstream 100 bases:

>100_bases
CCCTCAGGGTAGCGGAAACGCGCCGGTTTCGTCAAGGCGTGCCATCCGGGCATTCAGGTCTCTCCTTGTCCGGATGCCCG
ACTGCTGGTACCATGGGGCC

Downstream 100 bases:

>100_bases
ACCCCCATGCCCCTCGTTTAGATTTGATTTTTCCCGCCGCAATTGGCTATTATCAAAAAGATTAACCCATTACGGAAGGT
TCAGACCATGGTACGCACGA

Product: primosomal protein n`

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 751; Mature: 750

Protein sequence:

>751_residues
MSSFPVNERQTVIVHVAVPLPLDAPFSYRVPAPLAPAAAVGRRLLVPFGRRKVTGYVLEVADDDGRELRDVLEFLDDGPL
FTPGELALYRWASSYYLHPLGEVIKAALPAGINVQSRRGAGLDEGELTGGRSVKTERVYRAVAEVPDTVPPPGKASELLA
HLAEREEESASELRRLFGECSVQLRRLRELGFVEMAEREIYRDPFRMEAVGHDRPLELNERQSAALVRLRAALDAGRFTP
FLLRGVTGSGKTEVYLQAIAHCLEQGRSALVLVPEIALTPQLVSRFRRRFRCGVAVLHSGLSDGERFDEWRRIRRGEAAI
VIGARSAIFAPLADLGMIVVDEEHEGSYKQGEGFRYNARDMALVRGKMAGACVVLGTATPQVTTWYAVAGGKLECLDLPE
RVNGLPLPSAEIIDVRGARGHVLHPRLAAEVEANLARGEQTLLFLNRRGFSTWLTCEECGHVLRCPNCQVTLTYHQGRNR
HICHYCDYAVPAPSMCPGCGCGRIIHLGLGTEQVEAYVREHFPHARVDRMDRDTTARRGGHARILRKVAERQTDILIGTQ
MVAKGHDFPGITLVGILSVESTLNIPDYRSAERTFQLVTQLIGRAGRGDDPGRVLIQSLNPDHYALAHAVAGDMDGFYGA
ELAFRQETGYPPFAHLAALCLSGTAASAVERTSELLAARLRELRRDGGGRVEILGPAPAPLAKLRGRFRRQILLKAAGRT
ALHRLVCRARESFQPQAGVRLLVDIDPVDLM

Sequences:

>Translated_751_residues
MSSFPVNERQTVIVHVAVPLPLDAPFSYRVPAPLAPAAAVGRRLLVPFGRRKVTGYVLEVADDDGRELRDVLEFLDDGPL
FTPGELALYRWASSYYLHPLGEVIKAALPAGINVQSRRGAGLDEGELTGGRSVKTERVYRAVAEVPDTVPPPGKASELLA
HLAEREEESASELRRLFGECSVQLRRLRELGFVEMAEREIYRDPFRMEAVGHDRPLELNERQSAALVRLRAALDAGRFTP
FLLRGVTGSGKTEVYLQAIAHCLEQGRSALVLVPEIALTPQLVSRFRRRFRCGVAVLHSGLSDGERFDEWRRIRRGEAAI
VIGARSAIFAPLADLGMIVVDEEHEGSYKQGEGFRYNARDMALVRGKMAGACVVLGTATPQVTTWYAVAGGKLECLDLPE
RVNGLPLPSAEIIDVRGARGHVLHPRLAAEVEANLARGEQTLLFLNRRGFSTWLTCEECGHVLRCPNCQVTLTYHQGRNR
HICHYCDYAVPAPSMCPGCGCGRIIHLGLGTEQVEAYVREHFPHARVDRMDRDTTARRGGHARILRKVAERQTDILIGTQ
MVAKGHDFPGITLVGILSVESTLNIPDYRSAERTFQLVTQLIGRAGRGDDPGRVLIQSLNPDHYALAHAVAGDMDGFYGA
ELAFRQETGYPPFAHLAALCLSGTAASAVERTSELLAARLRELRRDGGGRVEILGPAPAPLAKLRGRFRRQILLKAAGRT
ALHRLVCRARESFQPQAGVRLLVDIDPVDLM
>Mature_750_residues
SSFPVNERQTVIVHVAVPLPLDAPFSYRVPAPLAPAAAVGRRLLVPFGRRKVTGYVLEVADDDGRELRDVLEFLDDGPLF
TPGELALYRWASSYYLHPLGEVIKAALPAGINVQSRRGAGLDEGELTGGRSVKTERVYRAVAEVPDTVPPPGKASELLAH
LAEREEESASELRRLFGECSVQLRRLRELGFVEMAEREIYRDPFRMEAVGHDRPLELNERQSAALVRLRAALDAGRFTPF
LLRGVTGSGKTEVYLQAIAHCLEQGRSALVLVPEIALTPQLVSRFRRRFRCGVAVLHSGLSDGERFDEWRRIRRGEAAIV
IGARSAIFAPLADLGMIVVDEEHEGSYKQGEGFRYNARDMALVRGKMAGACVVLGTATPQVTTWYAVAGGKLECLDLPER
VNGLPLPSAEIIDVRGARGHVLHPRLAAEVEANLARGEQTLLFLNRRGFSTWLTCEECGHVLRCPNCQVTLTYHQGRNRH
ICHYCDYAVPAPSMCPGCGCGRIIHLGLGTEQVEAYVREHFPHARVDRMDRDTTARRGGHARILRKVAERQTDILIGTQM
VAKGHDFPGITLVGILSVESTLNIPDYRSAERTFQLVTQLIGRAGRGDDPGRVLIQSLNPDHYALAHAVAGDMDGFYGAE
LAFRQETGYPPFAHLAALCLSGTAASAVERTSELLAARLRELRRDGGGRVEILGPAPAPLAKLRGRFRRQILLKAAGRTA
LHRLVCRARESFQPQAGVRLLVDIDPVDLM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=759, Percent_Identity=37.9446640316205, Blast_Score=449, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82649; Mature: 82518

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFPVNERQTVIVHVAVPLPLDAPFSYRVPAPLAPAAAVGRRLLVPFGRRKVTGYVLEV
CCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCEEECCCCCCEEEEEEEEE
ADDDGRELRDVLEFLDDGPLFTPGELALYRWASSYYLHPLGEVIKAALPAGINVQSRRGA
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCC
GLDEGELTGGRSVKTERVYRAVAEVPDTVPPPGKASELLAHLAEREEESASELRRLFGEC
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SVQLRRLRELGFVEMAEREIYRDPFRMEAVGHDRPLELNERQSAALVRLRAALDAGRFTP
HHHHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
FLLRGVTGSGKTEVYLQAIAHCLEQGRSALVLVPEIALTPQLVSRFRRRFRCGVAVLHSG
HHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHHHHHHHHHHHHCC
LSDGERFDEWRRIRRGEAAIVIGARSAIFAPLADLGMIVVDEEHEGSYKQGEGFRYNARD
CCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCEEEEECCCCCCCCCCCCEEECCCH
MALVRGKMAGACVVLGTATPQVTTWYAVAGGKLECLDLPERVNGLPLPSAEIIDVRGARG
HHHHHCCCCCEEEEEECCCCCEEEEEEECCCEEEEECCHHHCCCCCCCCCCEEEECCCCC
HVLHPRLAAEVEANLARGEQTLLFLNRRGFSTWLTCEECGHVLRCPNCQVTLTYHQGRNR
CEECCHHHHHHHHHHHCCCEEEEEEECCCCCEEEEHHHCCCEEECCCCEEEEEEECCCCC
HICHYCDYAVPAPSMCPGCGCGRIIHLGLGTEQVEAYVREHFPHARVDRMDRDTTARRGG
CEEEECCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCHHHHHCCCHHHHHCCC
HARILRKVAERQTDILIGTQMVAKGHDFPGITLVGILSVESTLNIPDYRSAERTFQLVTQ
HHHHHHHHHHHHCCEEEEHHHHHCCCCCCCEEEEEEEEHHCCCCCCCCCCHHHHHHHHHH
LIGRAGRGDDPGRVLIQSLNPDHYALAHAVAGDMDGFYGAELAFRQETGYPPFAHLAALC
HHHCCCCCCCCHHEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHH
LSGTAASAVERTSELLAARLRELRRDGGGRVEILGPAPAPLAKLRGRFRRQILLKAAGRT
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
ALHRLVCRARESFQPQAGVRLLVDIDPVDLM
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SSFPVNERQTVIVHVAVPLPLDAPFSYRVPAPLAPAAAVGRRLLVPFGRRKVTGYVLEV
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCEEECCCCCCEEEEEEEEE
ADDDGRELRDVLEFLDDGPLFTPGELALYRWASSYYLHPLGEVIKAALPAGINVQSRRGA
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCC
GLDEGELTGGRSVKTERVYRAVAEVPDTVPPPGKASELLAHLAEREEESASELRRLFGEC
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SVQLRRLRELGFVEMAEREIYRDPFRMEAVGHDRPLELNERQSAALVRLRAALDAGRFTP
HHHHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
FLLRGVTGSGKTEVYLQAIAHCLEQGRSALVLVPEIALTPQLVSRFRRRFRCGVAVLHSG
HHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHHHHHHHHHHHHCC
LSDGERFDEWRRIRRGEAAIVIGARSAIFAPLADLGMIVVDEEHEGSYKQGEGFRYNARD
CCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCEEEEECCCCCCCCCCCCEEECCCH
MALVRGKMAGACVVLGTATPQVTTWYAVAGGKLECLDLPERVNGLPLPSAEIIDVRGARG
HHHHHCCCCCEEEEEECCCCCEEEEEEECCCEEEEECCHHHCCCCCCCCCCEEEECCCCC
HVLHPRLAAEVEANLARGEQTLLFLNRRGFSTWLTCEECGHVLRCPNCQVTLTYHQGRNR
CEECCHHHHHHHHHHHCCCEEEEEEECCCCCEEEEHHHCCCEEECCCCEEEEEEECCCCC
HICHYCDYAVPAPSMCPGCGCGRIIHLGLGTEQVEAYVREHFPHARVDRMDRDTTARRGG
CEEEECCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCHHHHHCCCHHHHHCCC
HARILRKVAERQTDILIGTQMVAKGHDFPGITLVGILSVESTLNIPDYRSAERTFQLVTQ
HHHHHHHHHHHHCCEEEEHHHHHCCCCCCCEEEEEEEEHHCCCCCCCCCCHHHHHHHHHH
LIGRAGRGDDPGRVLIQSLNPDHYALAHAVAGDMDGFYGAELAFRQETGYPPFAHLAALC
HHHCCCCCCCCHHEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHH
LSGTAASAVERTSELLAARLRELRRDGGGRVEILGPAPAPLAKLRGRFRRQILLKAAGRT
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
ALHRLVCRARESFQPQAGVRLLVDIDPVDLM
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]