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The map label for this gene is hupB [H]

Identifier: 39995233

GI number: 39995233

Start: 135537

End: 137231

Strand: Reverse

Name: hupB [H]

Synonym: GSU0122

Alternate gene names: 39995233

Gene position: 137231-135537 (Counterclockwise)

Preceding gene: 39995234

Following gene: 39995232

Centisome position: 3.6

GC content: 63.3

Gene sequence:

>1695_bases
ATGATCGTTGTTGACCCGATCACGAGAATCGAGGGACATCTGCGCATCCAGGCCGAGGTGAACGGCGGACGGATCACCGA
TGCCTGGAGCTCCTGCACCATGTTCCGTGGGATCGAAAAAATCCTGAAGGGGCGCGATCCCCGCGACGCCTGGTATTTCA
CTCAGCGTTTCTGCGGCGTCTGCACCACCGTGCACTCCATCGCCTCCATCCGGGCGGTGGAAAATGCCCTGAACGTGAAG
ATCCCCCTGAACGCCGAACTGATCCGCAACCTCATCATCGGGAGCCAGGTGGTTCAGGATCACGTTATTCACTTCTACCA
TCTCCACGCCCTGGACTGGGTCGACGTGGTCTCGGCCCTCAAAGCCGATCCATCAAAGACGGCGACCCTGGCGGCTTCCA
TCTCTGACTGGCCCCTCAATTCTCCCACCTACTTCAAGTCGATCCAGGACCGGCTGGCGGCCTTTGTCAACAAGGGACGA
CTGGGCCCCTTCGGCAATGCCTACTGGGGTCACCCGGCCTACAAGTTGCCGCCCGAGGCGAACCTCATGGCCACGGCCCA
CTACCTCGAGGCGCTGGACTGGCAGCGGGAGATCATCAAGATCCACGCCATCCTCGGGTCCAAGAACCCCCATCCCCAGA
CCTTCCTGGTGGGCGGCATGGCGGTGCCGATAGATCCGAACTCTCAGAACGCCCTCAACGCAGACAAGATCGCCGAGATC
GGCCAACTCTTGAAAAAGACCCGGGCCTTCGTGGAAAAGGTCTACATCCCCGACCTGCTGGCGGTGGCGTCCTTCTATAC
AGAATGGGCCGGCATCGGCGGCGGGGTCGGGAACTACCTCTCCTACGGCGAATATCCCCTGGACAACAGCGGCAAACCCG
AAAAGCTCTGGCTCCCCCAAGGGATCATTCTGAATCGGGATCTGTCCCGGGTCTACCCCATCGACCAGCGTAAGATCACC
GAGAGCGTGGCTCACTCCTGGTACGAGGGGGAAGCCGCGGGGGTCCACCCCTATGACGAGACGACCGAGGCCAGGTACAC
CGGGCCGACACCGCCGTACGAGATGCTCGACACCGACGGCAAGTATTCCTGGGTAAAGTCGCCCCGTTACGACGGCAAAC
CCATGGAAACCGGGCCCCTGGCGACCATTTTGGTAGCCTACGCGGCAGGCCACCCCGGGGCAAGAGGCGCGGTGGACATG
GTCCTCGGCAAGCTCGGCGCGGGCCCCGAAGCACTCTTCTCAACCCTGGGACGTACCGCGGCCAGGGGAATCGAGACCCT
GCTCGTGGCCCGCGAACTCTCCTCGTGGCTCGACATGCTCGTGGCCAACATCGCCGGTGGCGACCTCACCATCCACAACG
GCGAAAAGTGGGACCCGGCCACCTGGCCCCGGGAGGCTGCCGGGTATGGCTGGCACAATGCCCCCCGCGGCGCGCTCGGG
CACTGGATCAGGATCAAAGACCAGAAGATCGAGAACTACCAGGCCGTGGTTCCCTCCACCTGGAACGCCTCGCCCCGGGA
TGAAAAGGGGCAGCTGGGCCCCTACGAGGCTGCGCTGGTAGGGACTCCCATGGCGGACCCCGGCAGGCCACTGGAGATCC
TCCGGACCATCCACTCCTTCGACCCGTGCTTGGCCTGCGCCGTGCACGTGGTCGACCCGAAAGGAGGAGAACTGGTCAGC
GTGAAGGTCCTGTAG

Upstream 100 bases:

>100_bases
CGCAGCGGCCACAGCGGCCGCATTCGGCATCCACGGCGTGGTGAGCGCCCTGCGCAAGGGGAACGATTCCGACGATGAGA
AGGAGGGGTAGCTCATGGCC

Downstream 100 bases:

>100_bases
GAGGAATTCATGGCACGGAACCGGAATTGTCTCTACGAGGAGTACGTCTGGGAGGTCCCGGTGCGGGTGACCCACTGGGT
CAACATGCTCGCCATCGTCG

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MIVVDPITRIEGHLRIQAEVNGGRITDAWSSCTMFRGIEKILKGRDPRDAWYFTQRFCGVCTTVHSIASIRAVENALNVK
IPLNAELIRNLIIGSQVVQDHVIHFYHLHALDWVDVVSALKADPSKTATLAASISDWPLNSPTYFKSIQDRLAAFVNKGR
LGPFGNAYWGHPAYKLPPEANLMATAHYLEALDWQREIIKIHAILGSKNPHPQTFLVGGMAVPIDPNSQNALNADKIAEI
GQLLKKTRAFVEKVYIPDLLAVASFYTEWAGIGGGVGNYLSYGEYPLDNSGKPEKLWLPQGIILNRDLSRVYPIDQRKIT
ESVAHSWYEGEAAGVHPYDETTEARYTGPTPPYEMLDTDGKYSWVKSPRYDGKPMETGPLATILVAYAAGHPGARGAVDM
VLGKLGAGPEALFSTLGRTAARGIETLLVARELSSWLDMLVANIAGGDLTIHNGEKWDPATWPREAAGYGWHNAPRGALG
HWIRIKDQKIENYQAVVPSTWNASPRDEKGQLGPYEAALVGTPMADPGRPLEILRTIHSFDPCLACAVHVVDPKGGELVS
VKVL

Sequences:

>Translated_564_residues
MIVVDPITRIEGHLRIQAEVNGGRITDAWSSCTMFRGIEKILKGRDPRDAWYFTQRFCGVCTTVHSIASIRAVENALNVK
IPLNAELIRNLIIGSQVVQDHVIHFYHLHALDWVDVVSALKADPSKTATLAASISDWPLNSPTYFKSIQDRLAAFVNKGR
LGPFGNAYWGHPAYKLPPEANLMATAHYLEALDWQREIIKIHAILGSKNPHPQTFLVGGMAVPIDPNSQNALNADKIAEI
GQLLKKTRAFVEKVYIPDLLAVASFYTEWAGIGGGVGNYLSYGEYPLDNSGKPEKLWLPQGIILNRDLSRVYPIDQRKIT
ESVAHSWYEGEAAGVHPYDETTEARYTGPTPPYEMLDTDGKYSWVKSPRYDGKPMETGPLATILVAYAAGHPGARGAVDM
VLGKLGAGPEALFSTLGRTAARGIETLLVARELSSWLDMLVANIAGGDLTIHNGEKWDPATWPREAAGYGWHNAPRGALG
HWIRIKDQKIENYQAVVPSTWNASPRDEKGQLGPYEAALVGTPMADPGRPLEILRTIHSFDPCLACAVHVVDPKGGELVS
VKVL
>Mature_564_residues
MIVVDPITRIEGHLRIQAEVNGGRITDAWSSCTMFRGIEKILKGRDPRDAWYFTQRFCGVCTTVHSIASIRAVENALNVK
IPLNAELIRNLIIGSQVVQDHVIHFYHLHALDWVDVVSALKADPSKTATLAASISDWPLNSPTYFKSIQDRLAAFVNKGR
LGPFGNAYWGHPAYKLPPEANLMATAHYLEALDWQREIIKIHAILGSKNPHPQTFLVGGMAVPIDPNSQNALNADKIAEI
GQLLKKTRAFVEKVYIPDLLAVASFYTEWAGIGGGVGNYLSYGEYPLDNSGKPEKLWLPQGIILNRDLSRVYPIDQRKIT
ESVAHSWYEGEAAGVHPYDETTEARYTGPTPPYEMLDTDGKYSWVKSPRYDGKPMETGPLATILVAYAAGHPGARGAVDM
VLGKLGAGPEALFSTLGRTAARGIETLLVARELSSWLDMLVANIAGGDLTIHNGEKWDPATWPREAAGYGWHNAPRGALG
HWIRIKDQKIENYQAVVPSTWNASPRDEKGQLGPYEAALVGTPMADPGRPLEILRTIHSFDPCLACAVHVVDPKGGELVS
VKVL

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=579, Percent_Identity=49.3955094991364, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI1789368, Length=576, Percent_Identity=48.2638888888889, Blast_Score=539, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 61911; Mature: 61911

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVVDPITRIEGHLRIQAEVNGGRITDAWSSCTMFRGIEKILKGRDPRDAWYFTQRFCGV
CEEECCHHHCCCEEEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
CTTVHSIASIRAVENALNVKIPLNAELIRNLIIGSQVVQDHVIHFYHLHALDWVDVVSAL
HHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KADPSKTATLAASISDWPLNSPTYFKSIQDRLAAFVNKGRLGPFGNAYWGHPAYKLPPEA
HCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
NLMATAHYLEALDWQREIIKIHAILGSKNPHPQTFLVGGMAVPIDPNSQNALNADKIAEI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCCCCCCCCCHHHHHHH
GQLLKKTRAFVEKVYIPDLLAVASFYTEWAGIGGGVGNYLSYGEYPLDNSGKPEKLWLPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCEEECCC
GIILNRDLSRVYPIDQRKITESVAHSWYEGEAAGVHPYDETTEARYTGPTPPYEMLDTDG
CEEECCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
KYSWVKSPRYDGKPMETGPLATILVAYAAGHPGARGAVDMVLGKLGAGPEALFSTLGRTA
CEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
ARGIETLLVARELSSWLDMLVANIAGGDLTIHNGEKWDPATWPREAAGYGWHNAPRGALG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHCCCCCCCCCCCCCC
HWIRIKDQKIENYQAVVPSTWNASPRDEKGQLGPYEAALVGTPMADPGRPLEILRTIHSF
CEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCC
DPCLACAVHVVDPKGGELVSVKVL
CHHHHHEEEEECCCCCCEEEEEEC
>Mature Secondary Structure
MIVVDPITRIEGHLRIQAEVNGGRITDAWSSCTMFRGIEKILKGRDPRDAWYFTQRFCGV
CEEECCHHHCCCEEEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
CTTVHSIASIRAVENALNVKIPLNAELIRNLIIGSQVVQDHVIHFYHLHALDWVDVVSAL
HHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KADPSKTATLAASISDWPLNSPTYFKSIQDRLAAFVNKGRLGPFGNAYWGHPAYKLPPEA
HCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
NLMATAHYLEALDWQREIIKIHAILGSKNPHPQTFLVGGMAVPIDPNSQNALNADKIAEI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCCCCCCCCCHHHHHHH
GQLLKKTRAFVEKVYIPDLLAVASFYTEWAGIGGGVGNYLSYGEYPLDNSGKPEKLWLPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCEEECCC
GIILNRDLSRVYPIDQRKITESVAHSWYEGEAAGVHPYDETTEARYTGPTPPYEMLDTDG
CEEECCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
KYSWVKSPRYDGKPMETGPLATILVAYAAGHPGARGAVDMVLGKLGAGPEALFSTLGRTA
CEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
ARGIETLLVARELSSWLDMLVANIAGGDLTIHNGEKWDPATWPREAAGYGWHNAPRGALG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHCCCCCCCCCCCCCC
HWIRIKDQKIENYQAVVPSTWNASPRDEKGQLGPYEAALVGTPMADPGRPLEILRTIHSF
CEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCC
DPCLACAVHVVDPKGGELVSVKVL
CHHHHHEEEEECCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275; 8163174 [H]