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The map label for this gene is sphR [H]

Identifier: 57235029

GI number: 57235029

Start: 132882

End: 133574

Strand: Direct

Name: sphR [H]

Synonym: DET0135

Alternate gene names: 57235029

Gene position: 132882-133574 (Clockwise)

Preceding gene: 57235046

Following gene: 57235028

Centisome position: 9.04

GC content: 52.81

Gene sequence:

>693_bases
ATGCCTGAGCATAAAATACTGGTAGTTGAAGATGACGCCACCTTGCGTGAGCTTTTGTGTTACAACCTTGCCAAGGAGGG
ATACCAGCTGGAATGTGCTGAAGACGGGCTTAAGGCGCTTGGTATGTACCGCCGGTTTAAGCCGTCTTTGGTTATACTGG
ACGTAATGCTGCCGGGCATGGACGGCTTTGAACTTTGCCGCCTTATCCGCGCTGAGAGCAGCGTGCCCATACTTATGCTG
ACCGCCCGTTCAAGCGAAACAGATAAAGTAAACGGGCTGGAAGTGGGGGCGGATGATTATCTGTCCAAACCCTTTGGCAT
GAAGGAACTGCTGGCCAGAATGAGGGCACTCCTCCGCCGCAGTCAGATGCAGAACTCCCAGCCAGAGCCTGTGAGCGGCC
TGAAAATAGATAATCTGGAAATATTTTTTGAACGTCATCTGGTCATGCGGGACGGGGAGGCTTTAGACTTTTCCCCGAAG
GAGTTTGACCTGCTGAGTTTTATGGTTCAGAACCGGTGCCGGGTTTTCAGCCGCGAACAGCTGCTGGAAAAGGTTTGGGG
TTATGATTATCCGGGCGGTACCCGCACGGTTGATGTACATATCAGCTGGCTGCGCCAGAAAATAGAGACTGACCCGTCCC
GTCCCCGCCACCTGATAACAGTGCGGGGAACCGGCTATAAGTTTGAGGAGTAA

Upstream 100 bases:

>100_bases
TTATATAGCCAATAGTACTAAAACCGGGTAAACATTTTCAATACCTGTCTGATGAAATGCCTGTCTTGCTGAAAATAGTC
TCTGGCTTTATACTTTAGCC

Downstream 100 bases:

>100_bases
ATATGGCCAGAAGCCTTAAATGGCGTTTAATAAGCCTGTTTTGCCTGCTTATTGCCGCTAGCGTGGGTCTGCTCAGCATT
TTCCTGTCCAATCATCTGGA

Product: DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MPEHKILVVEDDATLRELLCYNLAKEGYQLECAEDGLKALGMYRRFKPSLVILDVMLPGMDGFELCRLIRAESSVPILML
TARSSETDKVNGLEVGADDYLSKPFGMKELLARMRALLRRSQMQNSQPEPVSGLKIDNLEIFFERHLVMRDGEALDFSPK
EFDLLSFMVQNRCRVFSREQLLEKVWGYDYPGGTRTVDVHISWLRQKIETDPSRPRHLITVRGTGYKFEE

Sequences:

>Translated_230_residues
MPEHKILVVEDDATLRELLCYNLAKEGYQLECAEDGLKALGMYRRFKPSLVILDVMLPGMDGFELCRLIRAESSVPILML
TARSSETDKVNGLEVGADDYLSKPFGMKELLARMRALLRRSQMQNSQPEPVSGLKIDNLEIFFERHLVMRDGEALDFSPK
EFDLLSFMVQNRCRVFSREQLLEKVWGYDYPGGTRTVDVHISWLRQKIETDPSRPRHLITVRGTGYKFEE
>Mature_229_residues
PEHKILVVEDDATLRELLCYNLAKEGYQLECAEDGLKALGMYRRFKPSLVILDVMLPGMDGFELCRLIRAESSVPILMLT
ARSSETDKVNGLEVGADDYLSKPFGMKELLARMRALLRRSQMQNSQPEPVSGLKIDNLEIFFERHLVMRDGEALDFSPKE
FDLLSFMVQNRCRVFSREQLLEKVWGYDYPGGTRTVDVHISWLRQKIETDPSRPRHLITVRGTGYKFEE

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=44.7368421052632, Blast_Score=176, Evalue=9e-46,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=39.7321428571429, Blast_Score=168, Evalue=2e-43,
Organism=Escherichia coli, GI87082012, Length=224, Percent_Identity=38.8392857142857, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1789809, Length=230, Percent_Identity=38.695652173913, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=40.3508771929825, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1790860, Length=229, Percent_Identity=39.3013100436681, Blast_Score=147, Evalue=4e-37,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=36.7256637168142, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1790863, Length=227, Percent_Identity=36.1233480176211, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1790552, Length=229, Percent_Identity=42.7947598253275, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI2367329, Length=226, Percent_Identity=40.2654867256637, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1787375, Length=227, Percent_Identity=36.5638766519824, Blast_Score=130, Evalue=5e-32,
Organism=Escherichia coli, GI1789402, Length=227, Percent_Identity=35.6828193832599, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=33.1877729257642, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI145693140, Length=233, Percent_Identity=33.4763948497854, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788550, Length=128, Percent_Identity=33.59375, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788191, Length=126, Percent_Identity=36.5079365079365, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790299, Length=127, Percent_Identity=32.2834645669291, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=37.8640776699029, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788713, Length=119, Percent_Identity=37.8151260504202, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790437, Length=129, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787487, Length=101, Percent_Identity=34.6534653465347, Blast_Score=61, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=106, Percent_Identity=31.1320754716981, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26521; Mature: 26390

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEHKILVVEDDATLRELLCYNLAKEGYQLECAEDGLKALGMYRRFKPSLVILDVMLPGM
CCCCEEEEEECCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCC
DGFELCRLIRAESSVPILMLTARSSETDKVNGLEVGADDYLSKPFGMKELLARMRALLRR
CHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHH
SQMQNSQPEPVSGLKIDNLEIFFERHLVMRDGEALDFSPKEFDLLSFMVQNRCRVFSREQ
HHHCCCCCCCCCCEEECCCCEEEEHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLEKVWGYDYPGGTRTVDVHISWLRQKIETDPSRPRHLITVRGTGYKFEE
HHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
PEHKILVVEDDATLRELLCYNLAKEGYQLECAEDGLKALGMYRRFKPSLVILDVMLPGM
CCCEEEEEECCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCC
DGFELCRLIRAESSVPILMLTARSSETDKVNGLEVGADDYLSKPFGMKELLARMRALLRR
CHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHH
SQMQNSQPEPVSGLKIDNLEIFFERHLVMRDGEALDFSPKEFDLLSFMVQNRCRVFSREQ
HHHCCCCCCCCCCEEECCCCEEEEHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLEKVWGYDYPGGTRTVDVHISWLRQKIETDPSRPRHLITVRGTGYKFEE
HHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]