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The map label for this gene is ksgA

Identifier: 57234784

GI number: 57234784

Start: 382877

End: 383752

Strand: Direct

Name: ksgA

Synonym: DET0404

Alternate gene names: 57234784

Gene position: 382877-383752 (Clockwise)

Preceding gene: 57234785

Following gene: 57234783

Centisome position: 26.05

GC content: 51.6

Gene sequence:

>876_bases
ATGGAGAAAGACGCACCTCTGGCAGCTACTGGTACCCCTTCACTTATGGCTCAGGCCAAAGAGATGATGGAGGGCTATAC
CCTCAGGGCCCGCAAAGGGCTGGGGCAGCATTTCCTGATAAGCCAGGGCGTGCTTAACAAAATACTACTGGCTGCAGATT
TGAAACCCACGGATACGGTTATTGAAGTGGGGCCGGGGCTTGGAGTGCTTACCGAAGAACTCATTAAAAGGGCCGGGCAG
GTTATAGCGGTAGAACTGGATGACAAGCTGGTAACTGCCTTGACCGAAAAATTTAAAGCCTATCCTAATTTCAGGATTAT
CCATTCAGATATACTTAAAACCAGCCCGGCGGAAATACTGGGTCAAAATATTCCCTATAAGCTGGTGGCCAATCTTCCGT
ATTACATAACCTCAGCCGTACTTCGCCAGTTTCTGGAAGCTGAGGCCAAGCCGGAACTTATGGTGGTCATGGTACAAAAA
GAGGTGGCCAAAAATATGGTCGCCCAAACAGGGGACATGGGTCTTTTAACCCTGAGTGTGCGTTTTTACGGCAACCCTTC
GCTGGTATCAGTAGTGCCGGGCGGGGCTTTTTATCCTCCGCCGGAAGTGGATTCGGCTATTGTAAAGGTAGCTATCCCCC
AGGCAGCCATTATGCAGGGGGTGTCTGAGGCTGATTTTTTTAAACTGGCCAGAGCCGGTTTCGGCACCCGCCGTAAAACC
CTTCTTAACGCTCTGGCACAGGGACTGGGTGTATCCAAGCAGAGTGTACTGGCACTGCTGAACAGGGCAGGCATAGAACC
CGCCCGCCGGGCAGAGACCTTGAGTATGGAGGAGTGGAAAATGTTATGCCTGGTGTACACGGAGAACCCATGCTGA

Upstream 100 bases:

>100_bases
AACATAACGGCTACCCAGAAAAAGATGCAAAAAGCCAAATTGCCCCAGTTTTTGTCAGCACGATTGAGCAAAGGGAGCTA
AATCCCTATAATAGGGGTAC

Downstream 100 bases:

>100_bases
CACTGCTGGCTCCGGCCAAAGTCAACCTTAGTCTGGAAGTCCTCTACCGCCGGAATGACGGGTACCATGAGCTGCGGAGT
ATTATTCAGTCACTCAGCTT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MEKDAPLAATGTPSLMAQAKEMMEGYTLRARKGLGQHFLISQGVLNKILLAADLKPTDTVIEVGPGLGVLTEELIKRAGQ
VIAVELDDKLVTALTEKFKAYPNFRIIHSDILKTSPAEILGQNIPYKLVANLPYYITSAVLRQFLEAEAKPELMVVMVQK
EVAKNMVAQTGDMGLLTLSVRFYGNPSLVSVVPGGAFYPPPEVDSAIVKVAIPQAAIMQGVSEADFFKLARAGFGTRRKT
LLNALAQGLGVSKQSVLALLNRAGIEPARRAETLSMEEWKMLCLVYTENPC

Sequences:

>Translated_291_residues
MEKDAPLAATGTPSLMAQAKEMMEGYTLRARKGLGQHFLISQGVLNKILLAADLKPTDTVIEVGPGLGVLTEELIKRAGQ
VIAVELDDKLVTALTEKFKAYPNFRIIHSDILKTSPAEILGQNIPYKLVANLPYYITSAVLRQFLEAEAKPELMVVMVQK
EVAKNMVAQTGDMGLLTLSVRFYGNPSLVSVVPGGAFYPPPEVDSAIVKVAIPQAAIMQGVSEADFFKLARAGFGTRRKT
LLNALAQGLGVSKQSVLALLNRAGIEPARRAETLSMEEWKMLCLVYTENPC
>Mature_291_residues
MEKDAPLAATGTPSLMAQAKEMMEGYTLRARKGLGQHFLISQGVLNKILLAADLKPTDTVIEVGPGLGVLTEELIKRAGQ
VIAVELDDKLVTALTEKFKAYPNFRIIHSDILKTSPAEILGQNIPYKLVANLPYYITSAVLRQFLEAEAKPELMVVMVQK
EVAKNMVAQTGDMGLLTLSVRFYGNPSLVSVVPGGAFYPPPEVDSAIVKVAIPQAAIMQGVSEADFFKLARAGFGTRRKT
LLNALAQGLGVSKQSVLALLNRAGIEPARRAETLSMEEWKMLCLVYTENPC

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=216, Percent_Identity=32.4074074074074, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI156415992, Length=295, Percent_Identity=28.8135593220339, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1786236, Length=266, Percent_Identity=31.9548872180451, Blast_Score=122, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25146882, Length=212, Percent_Identity=33.4905660377358, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI25141369, Length=309, Percent_Identity=24.5954692556634, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324989, Length=252, Percent_Identity=28.968253968254, Blast_Score=102, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21357273, Length=300, Percent_Identity=29.6666666666667, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21358017, Length=215, Percent_Identity=31.1627906976744, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_DEHE1 (Q3Z9F0)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181149.1
- ProteinModelPortal:   Q3Z9F0
- SMR:   Q3Z9F0
- STRING:   Q3Z9F0
- GeneID:   3230253
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0404
- NMPDR:   fig|243164.3.peg.541
- TIGR:   DET0404
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- PhylomeDB:   Q3Z9F0
- ProtClustDB:   PRK00274
- BioCyc:   DETH243164:DET_0404-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31558; Mature: 31558

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 37-37 BINDING 39-39 BINDING 64-64 BINDING 85-85 BINDING 110-110 BINDING 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDAPLAATGTPSLMAQAKEMMEGYTLRARKGLGQHFLISQGVLNKILLAADLKPTDTV
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCE
IEVGPGLGVLTEELIKRAGQVIAVELDDKLVTALTEKFKAYPNFRIIHSDILKTSPAEIL
EEECCCCCHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCCCEEEEEHHHHCCCCHHHH
GQNIPYKLVANLPYYITSAVLRQFLEAEAKPELMVVMVQKEVAKNMVAQTGDMGLLTLSV
CCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCEEEEEEE
RFYGNPSLVSVVPGGAFYPPPEVDSAIVKVAIPQAAIMQGVSEADFFKLARAGFGTRRKT
EEECCCCEEEEECCCCCCCCCCCCHHHHEEECCHHHHHHCCCHHHHHHHHHHCCCHHHHH
LLNALAQGLGVSKQSVLALLNRAGIEPARRAETLSMEEWKMLCLVYTENPC
HHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHEEEEEEEEECCCC
>Mature Secondary Structure
MEKDAPLAATGTPSLMAQAKEMMEGYTLRARKGLGQHFLISQGVLNKILLAADLKPTDTV
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCE
IEVGPGLGVLTEELIKRAGQVIAVELDDKLVTALTEKFKAYPNFRIIHSDILKTSPAEIL
EEECCCCCHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCCCEEEEEHHHHCCCCHHHH
GQNIPYKLVANLPYYITSAVLRQFLEAEAKPELMVVMVQKEVAKNMVAQTGDMGLLTLSV
CCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCEEEEEEE
RFYGNPSLVSVVPGGAFYPPPEVDSAIVKVAIPQAAIMQGVSEADFFKLARAGFGTRRKT
EEECCCCEEEEECCCCCCCCCCCCHHHHEEECCHHHHHHCCCHHHHHHHHHHCCCHHHHH
LLNALAQGLGVSKQSVLALLNRAGIEPARRAETLSMEEWKMLCLVYTENPC
HHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA