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The map label for this gene is ispE

Identifier: 57234783

GI number: 57234783

Start: 383746

End: 384600

Strand: Direct

Name: ispE

Synonym: DET0405

Alternate gene names: 57234783

Gene position: 383746-384600 (Clockwise)

Preceding gene: 57234784

Following gene: 57234822

Centisome position: 26.11

GC content: 50.99

Gene sequence:

>855_bases
ATGCTGACACTGCTGGCTCCGGCCAAAGTCAACCTTAGTCTGGAAGTCCTCTACCGCCGGAATGACGGGTACCATGAGCT
GCGGAGTATTATTCAGTCACTCAGCTTATGTGACCGCCTGTCTTTCAGCCCGGCCAAAACAGTGCAAATAAGCTCAGACA
GCCCGGATTGGCAGGCTGAACATAGCTTGGTTTCCAAAGCTGTGGCCTTATTTAGTGAAAAGTGCGGGCAGGGACGCGGG
GTAAACCTGACTATAGCCAAGCGTATTCCCCTGGTTTCGGGGTTGGGGGGAGACAGTTCCTGTGCGGCGGCTGTTTTAAA
AGGGCTTAACAAGCTGTGGGGGTGCGGTTATCCGCGCTGGCGGCTTATGGAACTGGGGGCAGAGCTGGGTTCTGACGTGC
CGTTTTTCATGATGGGCGGTACGGCTATGATGGAAGGACGTGGAGAAACCGTAACCCCCCTGCCCACTCTCAATCAAATG
TGGGCGGTTTTGCTGGTTCCTGAAATAGAAATGCCGCCGGATAAAACTGCCGCTCTCTACCGTAATCTGCATGCAGATAG
CTTTAGCAGCGGGGAAATAAGCGATAAACTGCTGGAAGATATATGTCAGGGTAAGCTAAGCTACAGTTCGTGTTTCAATG
TATTTGAAAAAATAGCCTTCACCCTTTTCCCTGATTTGGCCAAATACCGCTGGCAATTTCTGGAAGCCGGGGCTTACCAG
ATATTTTTAGCCGGAGCAGGCCCGACCCTCTTTACCCTGCTTAAAGATAAAAATATGGCTGAGAAGATATACCATAATCT
GTGCCAGAAGGGGCATCAGGCTTATCTGGTATCCACTTTAGGGCCACTGGATTGA

Upstream 100 bases:

>100_bases
TGGCACTGCTGAACAGGGCAGGCATAGAACCCGCCCGCCGGGCAGAGACCTTGAGTATGGAGGAGTGGAAAATGTTATGC
CTGGTGTACACGGAGAACCC

Downstream 100 bases:

>100_bases
TGGGTGGCATAGAGGTATCATATAAATATCATTCAAATATCAGGGAGGTGTTTTGATGTCCAGTTCCGAAAATCTGTCGG
CTGCTTTTGCCGGTGAAAGC

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MLTLLAPAKVNLSLEVLYRRNDGYHELRSIIQSLSLCDRLSFSPAKTVQISSDSPDWQAEHSLVSKAVALFSEKCGQGRG
VNLTIAKRIPLVSGLGGDSSCAAAVLKGLNKLWGCGYPRWRLMELGAELGSDVPFFMMGGTAMMEGRGETVTPLPTLNQM
WAVLLVPEIEMPPDKTAALYRNLHADSFSSGEISDKLLEDICQGKLSYSSCFNVFEKIAFTLFPDLAKYRWQFLEAGAYQ
IFLAGAGPTLFTLLKDKNMAEKIYHNLCQKGHQAYLVSTLGPLD

Sequences:

>Translated_284_residues
MLTLLAPAKVNLSLEVLYRRNDGYHELRSIIQSLSLCDRLSFSPAKTVQISSDSPDWQAEHSLVSKAVALFSEKCGQGRG
VNLTIAKRIPLVSGLGGDSSCAAAVLKGLNKLWGCGYPRWRLMELGAELGSDVPFFMMGGTAMMEGRGETVTPLPTLNQM
WAVLLVPEIEMPPDKTAALYRNLHADSFSSGEISDKLLEDICQGKLSYSSCFNVFEKIAFTLFPDLAKYRWQFLEAGAYQ
IFLAGAGPTLFTLLKDKNMAEKIYHNLCQKGHQAYLVSTLGPLD
>Mature_284_residues
MLTLLAPAKVNLSLEVLYRRNDGYHELRSIIQSLSLCDRLSFSPAKTVQISSDSPDWQAEHSLVSKAVALFSEKCGQGRG
VNLTIAKRIPLVSGLGGDSSCAAAVLKGLNKLWGCGYPRWRLMELGAELGSDVPFFMMGGTAMMEGRGETVTPLPTLNQM
WAVLLVPEIEMPPDKTAALYRNLHADSFSSGEISDKLLEDICQGKLSYSSCFNVFEKIAFTLFPDLAKYRWQFLEAGAYQ
IFLAGAGPTLFTLLKDKNMAEKIYHNLCQKGHQAYLVSTLGPLD

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=175, Percent_Identity=38.2857142857143, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_DEHE1 (Q3Z9E9)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181150.1
- ProteinModelPortal:   Q3Z9E9
- SMR:   Q3Z9E9
- STRING:   Q3Z9E9
- GeneID:   3230252
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0405
- NMPDR:   fig|243164.3.peg.542
- TIGR:   DET0405
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   HEIVTIL
- PhylomeDB:   Q3Z9E9
- ProtClustDB:   CLSK837487
- BioCyc:   DETH243164:DET_0405-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31236; Mature: 31236

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: ACT_SITE 9-9 ACT_SITE 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLLAPAKVNLSLEVLYRRNDGYHELRSIIQSLSLCDRLSFSPAKTVQISSDSPDWQAE
CEEEECCCEECEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
HSLVSKAVALFSEKCGQGRGVNLTIAKRIPLVSGLGGDSSCAAAVLKGLNKLWGCGYPRW
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCHH
RLMELGAELGSDVPFFMMGGTAMMEGRGETVTPLPTLNQMWAVLLVPEIEMPPDKTAALY
HHHHHHHHHCCCCCEEEECCEEEECCCCCEECCCCCHHHHHHHHEECCCCCCCCHHHHHH
RNLHADSFSSGEISDKLLEDICQGKLSYSSCFNVFEKIAFTLFPDLAKYRWQFLEAGAYQ
HHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IFLAGAGPTLFTLLKDKNMAEKIYHNLCQKGHQAYLVSTLGPLD
EEEECCCCHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCC
>Mature Secondary Structure
MLTLLAPAKVNLSLEVLYRRNDGYHELRSIIQSLSLCDRLSFSPAKTVQISSDSPDWQAE
CEEEECCCEECEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
HSLVSKAVALFSEKCGQGRGVNLTIAKRIPLVSGLGGDSSCAAAVLKGLNKLWGCGYPRW
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCHH
RLMELGAELGSDVPFFMMGGTAMMEGRGETVTPLPTLNQMWAVLLVPEIEMPPDKTAALY
HHHHHHHHHCCCCCEEEECCEEEECCCCCEECCCCCHHHHHHHHEECCCCCCCCHHHHHH
RNLHADSFSSGEISDKLLEDICQGKLSYSSCFNVFEKIAFTLFPDLAKYRWQFLEAGAYQ
HHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IFLAGAGPTLFTLLKDKNMAEKIYHNLCQKGHQAYLVSTLGPLD
EEEECCCCHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA