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The map label for this gene is kamA [H]

Identifier: 57234545

GI number: 57234545

Start: 582476

End: 583795

Strand: Direct

Name: kamA [H]

Synonym: DET0628

Alternate gene names: 57234545

Gene position: 582476-583795 (Clockwise)

Preceding gene: 57234546

Following gene: 57234544

Centisome position: 39.63

GC content: 47.12

Gene sequence:

>1320_bases
GTGACCACTACCGAAGAAAATAAAAACACACAATCAGATGACCCTCTCGGCGCAGGTGCAGTCGAAACTTATGGCAAGGT
AGACGATGAGCCTCCCAGTAGGCTTGGCCAAACAAAAAGATTTAGACTTAAATATTTCCCCAAAGTAAGTGACCAGAACT
GGAATGACTGGCACTGGCAGTTTAAAAACCGTGTTACTTCTGTAACAGAAATAGCAAGGTTTTTTCATTTGTCTGCAGAA
GAATACAGGGATATGGAGGCCGTTTCTGCGGTTTTCCCCCTTTCCGCCACCCCTTATTACCTCAGTCTGGTAGATTTTGA
CAATGTAAATGACCCGGTAAAGTGTCAGCTTATGCCGGATACAGCTGAACTTAATTTTGATACCCGCTGCTGTTCTGACC
CTTTGGAAGAGGCTCATAGTTCGGTAGTGCCTGGGCTGGTACACCGTTATCCTGACAGGGTGGTCATGGTGCTGACTGAT
ATATGCCCGGTACTGTGCCGCCACTGCACCCGTAAAAGGGAATGGAAAAACGGCGGTTGGGTGCATACCCAGGCAGAGAT
AGATGCCATGCTGGCATATATACGCCAGCATCAGGTTATCCGGGACGTAATTATCTCCGGCGGTGACCCCTTAACCCTGT
CTACACCCCGCTTGGAAAGTGTTCTTTCGGCTCTGCGTTCAATACCCCATGTGGAAATTATCCGTATAGGTACCCGCTAT
CCTGTGGTCTTACCCCAGCGGATAGATGATGAACTATGCAATATGCTTTCCAAGTACGGTCCTATCTGGCTTAATACCCA
TTATAACCACCCTAATGAAATTACCGAAGAATCCCGTCAGGCCTGTGACCGTCTGGTCAGGGCGGGCGTACCTGTAAATA
ACCAGTCGGTACTTTTAAAAGGCATAAATGACAGTGTGTCTGTGCAGAAATCCCTTTGCCACAAGCTTTTAATGAGCAAA
GTGCGCCCTTACTACCTTTTCCAGTGTGACAATGTGCAGGGTACAGAGCATTTCCATACCCCCATTGAAACCGGGGTGGG
GATTATTGAGGGCTTGCGCGGTTATACCTCCGGTTTGGCTGTGCCGAATTACGTTATAGACTTACCGGGTGGCGGCGGCA
AAATAACTATTCAGCCGGACTACGTGCTGGATAAACAGGCTGACGAATATATAATCCGTAATTACAAGGGTGATATAATC
CGTTTTAAAAATCCGGGTGACCCCAAAGTTTACAGCAATACCGGCCGGACAGATTTACTTAATAATGCCGCTAAAATAAA
GAGAGAAACAGATGCAGATAGGGCTGTCATACGATCTTAA

Upstream 100 bases:

>100_bases
GCTTCCTTGACAGTAGCTATGTTTCGTGTAGAATATTACTCACAATTATACGTAATTGTGAGTAAACGCCATAAATTCTT
AAGGAGGCTTCAGGCCTAAC

Downstream 100 bases:

>100_bases
ACAGGCGTTAAATCCGGTTAGCGGCGGCCCTGATGATGCGCTGGAAGAATATGACTCACCGGAGACGGTTGAAGCTATCA
CTAATGCTTTAACCCAGGCC

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: LAM; KAM [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTTTEENKNTQSDDPLGAGAVETYGKVDDEPPSRLGQTKRFRLKYFPKVSDQNWNDWHWQFKNRVTSVTEIARFFHLSAE
EYRDMEAVSAVFPLSATPYYLSLVDFDNVNDPVKCQLMPDTAELNFDTRCCSDPLEEAHSSVVPGLVHRYPDRVVMVLTD
ICPVLCRHCTRKREWKNGGWVHTQAEIDAMLAYIRQHQVIRDVIISGGDPLTLSTPRLESVLSALRSIPHVEIIRIGTRY
PVVLPQRIDDELCNMLSKYGPIWLNTHYNHPNEITEESRQACDRLVRAGVPVNNQSVLLKGINDSVSVQKSLCHKLLMSK
VRPYYLFQCDNVQGTEHFHTPIETGVGIIEGLRGYTSGLAVPNYVIDLPGGGGKITIQPDYVLDKQADEYIIRNYKGDII
RFKNPGDPKVYSNTGRTDLLNNAAKIKRETDADRAVIRS

Sequences:

>Translated_439_residues
MTTTEENKNTQSDDPLGAGAVETYGKVDDEPPSRLGQTKRFRLKYFPKVSDQNWNDWHWQFKNRVTSVTEIARFFHLSAE
EYRDMEAVSAVFPLSATPYYLSLVDFDNVNDPVKCQLMPDTAELNFDTRCCSDPLEEAHSSVVPGLVHRYPDRVVMVLTD
ICPVLCRHCTRKREWKNGGWVHTQAEIDAMLAYIRQHQVIRDVIISGGDPLTLSTPRLESVLSALRSIPHVEIIRIGTRY
PVVLPQRIDDELCNMLSKYGPIWLNTHYNHPNEITEESRQACDRLVRAGVPVNNQSVLLKGINDSVSVQKSLCHKLLMSK
VRPYYLFQCDNVQGTEHFHTPIETGVGIIEGLRGYTSGLAVPNYVIDLPGGGGKITIQPDYVLDKQADEYIIRNYKGDII
RFKNPGDPKVYSNTGRTDLLNNAAKIKRETDADRAVIRS
>Mature_438_residues
TTTEENKNTQSDDPLGAGAVETYGKVDDEPPSRLGQTKRFRLKYFPKVSDQNWNDWHWQFKNRVTSVTEIARFFHLSAEE
YRDMEAVSAVFPLSATPYYLSLVDFDNVNDPVKCQLMPDTAELNFDTRCCSDPLEEAHSSVVPGLVHRYPDRVVMVLTDI
CPVLCRHCTRKREWKNGGWVHTQAEIDAMLAYIRQHQVIRDVIISGGDPLTLSTPRLESVLSALRSIPHVEIIRIGTRYP
VVLPQRIDDELCNMLSKYGPIWLNTHYNHPNEITEESRQACDRLVRAGVPVNNQSVLLKGINDSVSVQKSLCHKLLMSKV
RPYYLFQCDNVQGTEHFHTPIETGVGIIEGLRGYTSGLAVPNYVIDLPGGGGKITIQPDYVLDKQADEYIIRNYKGDIIR
FKNPGDPKVYSNTGRTDLLNNAAKIKRETDADRAVIRS

Specific function: Catalyzes the interconversion of L-alpha-lysine and L- beta-lysine [H]

COG id: COG1509

COG function: function code E; Lysine 2,3-aminomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KamA family [H]

Homologues:

Organism=Escherichia coli, GI1790589, Length=333, Percent_Identity=33.6336336336336, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003739
- InterPro:   IPR022459
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =5.4.3.2 [H]

Molecular weight: Translated: 49746; Mature: 49615

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTEENKNTQSDDPLGAGAVETYGKVDDEPPSRLGQTKRFRLKYFPKVSDQNWNDWHWQ
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCCHHHH
FKNRVTSVTEIARFFHLSAEEYRDMEAVSAVFPLSATPYYLSLVDFDNVNDPVKCQLMPD
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEECCC
TAELNFDTRCCSDPLEEAHSSVVPGLVHRYPDRVVMVLTDICPVLCRHCTRKREWKNGGW
CCCCCCCHHHCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VHTQAEIDAMLAYIRQHQVIRDVIISGGDPLTLSTPRLESVLSALRSIPHVEIIRIGTRY
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCEEEEEECCCC
PVVLPQRIDDELCNMLSKYGPIWLNTHYNHPNEITEESRQACDRLVRAGVPVNNQSVLLK
CEECCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
GINDSVSVQKSLCHKLLMSKVRPYYLFQCDNVQGTEHFHTPIETGVGIIEGLRGYTSGLA
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHCCCC
VPNYVIDLPGGGGKITIQPDYVLDKQADEYIIRNYKGDIIRFKNPGDPKVYSNTGRTDLL
CCCEEEECCCCCCEEEECCCCEECCCCHHHHHHCCCCCEEEECCCCCCCEECCCCCHHHH
NNAAKIKRETDADRAVIRS
HHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
TTTEENKNTQSDDPLGAGAVETYGKVDDEPPSRLGQTKRFRLKYFPKVSDQNWNDWHWQ
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCCHHHH
FKNRVTSVTEIARFFHLSAEEYRDMEAVSAVFPLSATPYYLSLVDFDNVNDPVKCQLMPD
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEECCC
TAELNFDTRCCSDPLEEAHSSVVPGLVHRYPDRVVMVLTDICPVLCRHCTRKREWKNGGW
CCCCCCCHHHCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
VHTQAEIDAMLAYIRQHQVIRDVIISGGDPLTLSTPRLESVLSALRSIPHVEIIRIGTRY
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCEEEEEECCCC
PVVLPQRIDDELCNMLSKYGPIWLNTHYNHPNEITEESRQACDRLVRAGVPVNNQSVLLK
CEECCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
GINDSVSVQKSLCHKLLMSKVRPYYLFQCDNVQGTEHFHTPIETGVGIIEGLRGYTSGLA
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHCCCC
VPNYVIDLPGGGGKITIQPDYVLDKQADEYIIRNYKGDIIRFKNPGDPKVYSNTGRTDLL
CCCEEEECCCCCCEEEECCCCEECCCCHHHHHHCCCCCEEEECCCCCCCEECCCCCHHHH
NNAAKIKRETDADRAVIRS
HHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10629195 [H]