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The map label for this gene is nuoG2 [H]

Identifier: 57234505

GI number: 57234505

Start: 673619

End: 674287

Strand: Direct

Name: nuoG2 [H]

Synonym: DET0730

Alternate gene names: 57234505

Gene position: 673619-674287 (Clockwise)

Preceding gene: 57234506

Following gene: 57234504

Centisome position: 45.83

GC content: 49.03

Gene sequence:

>669_bases
ATGATAAATTTAACCATAGACGGCAAGAAATATCAGGCAGAAGAAGGTCAGACAATACTGGAAGTAGCCAACCGGCATGG
TATTTTTATTCCTACCCTATGCGTAGATGAAGCGGTTTCAGCTTATGGAGCCTGCCGTTTGTGTATGGTGGAAATTGAGC
GGAAAGGCCGCAAAAAACTGGTGGCTTCCTGTCTGTATGAGGTTGAAGAGGGTTTGGCTGTCAGCACCCAGAATGAGCGG
ATTGCCAATATCCGCAAGACTGTTATAGAGCTGCTGCTGGCACGCTGTCCCCAGTCCGAGGGTGTTCAGGCTATGGCTAA
GAAACTGGGGGTTACCTGCTCCCGGTTTGAAAGTGAAAAACCTGAAAATCTGTGCACCTTATGCGCCCTTTGCACCAGAG
TTTGCTCTGAGGTAGTGGGTAAGAGCGCTATAAGCCTGGTAAGCAGGGGCACCAGCCGTGAAGTAGCCTTGCCCTATTAC
GAAGATAAAACAGATTGTATTGCCTGTGGTTCGTGTGCTTATATCTGCCCAACCGGGGCTATAAAGATGGAAGACGGGAA
TGGTATGCGTACTATTACCTGGCCTAACTGCCAGGTCAGCTTTAACTTAAGCGCTGTGCCAAGTGCGGCGCCGAATGGAT
TCCCGAAAAACAGGTCAAGCATATCTTGA

Upstream 100 bases:

>100_bases
GATGCCGGTCATACTTGACCAGAGCAAATGTACTAAATGCGGTGCGTGTTTTGATGCTTGTAAGCTCCACGCCGTAGAAA
TCAGATAAGGTAGGGTGGCT

Downstream 100 bases:

>100_bases
ATACCACCGAGGTTCCGGCAGAGTTTTTTGATATCTGCCCTGATTGCCGCTAGTCCTGGGGCAATCGTCCTTGACCCTAT
AATATAGCTTTGTTATACTG

Product: [Fe] hydrogenase, HymC subunit, putative

Products: NA

Alternate protein names: NADH dehydrogenase I subunit G 2; NDH-1 subunit G 2 [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MINLTIDGKKYQAEEGQTILEVANRHGIFIPTLCVDEAVSAYGACRLCMVEIERKGRKKLVASCLYEVEEGLAVSTQNER
IANIRKTVIELLLARCPQSEGVQAMAKKLGVTCSRFESEKPENLCTLCALCTRVCSEVVGKSAISLVSRGTSREVALPYY
EDKTDCIACGSCAYICPTGAIKMEDGNGMRTITWPNCQVSFNLSAVPSAAPNGFPKNRSSIS

Sequences:

>Translated_222_residues
MINLTIDGKKYQAEEGQTILEVANRHGIFIPTLCVDEAVSAYGACRLCMVEIERKGRKKLVASCLYEVEEGLAVSTQNER
IANIRKTVIELLLARCPQSEGVQAMAKKLGVTCSRFESEKPENLCTLCALCTRVCSEVVGKSAISLVSRGTSREVALPYY
EDKTDCIACGSCAYICPTGAIKMEDGNGMRTITWPNCQVSFNLSAVPSAAPNGFPKNRSSIS
>Mature_222_residues
MINLTIDGKKYQAEEGQTILEVANRHGIFIPTLCVDEAVSAYGACRLCMVEIERKGRKKLVASCLYEVEEGLAVSTQNER
IANIRKTVIELLLARCPQSEGVQAMAKKLGVTCSRFESEKPENLCTLCALCTRVCSEVVGKSAISLVSRGTSREVALPYY
EDKTDCIACGSCAYICPTGAIKMEDGNGMRTITWPNCQVSFNLSAVPSAAPNGFPKNRSSIS

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1034

COG function: function code C; NADH dehydrogenase/NADH:ubiquinone oxidoreductase 75 kD subunit (chain G)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI33519475, Length=204, Percent_Identity=30.3921568627451, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI145693161, Length=206, Percent_Identity=26.2135922330097, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193209088, Length=206, Percent_Identity=26.6990291262136, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32566231, Length=206, Percent_Identity=26.6990291262136, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17565758, Length=206, Percent_Identity=26.6990291262136, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24640559, Length=207, Percent_Identity=27.536231884058, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24640557, Length=207, Percent_Identity=27.536231884058, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR009010
- InterPro:   IPR012675
- InterPro:   IPR001041
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR000283
- InterPro:   IPR019574 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; PF10588 NADH-G_4Fe-4S_3 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 24087; Mature: 24087

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS51085 2FE2S_FER_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
6.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
9.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLTIDGKKYQAEEGQTILEVANRHGIFIPTLCVDEAVSAYGACRLCMVEIERKGRKKL
CEEEEECCCEECCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VASCLYEVEEGLAVSTQNERIANIRKTVIELLLARCPQSEGVQAMAKKLGVTCSRFESEK
HHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHCCCC
PENLCTLCALCTRVCSEVVGKSAISLVSRGTSREVALPYYEDKTDCIACGSCAYICPTGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEECCCEEEECCCCC
IKMEDGNGMRTITWPNCQVSFNLSAVPSAAPNGFPKNRSSIS
EEEECCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MINLTIDGKKYQAEEGQTILEVANRHGIFIPTLCVDEAVSAYGACRLCMVEIERKGRKKL
CEEEEECCCEECCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VASCLYEVEEGLAVSTQNERIANIRKTVIELLLARCPQSEGVQAMAKKLGVTCSRFESEK
HHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHCCCC
PENLCTLCALCTRVCSEVVGKSAISLVSRGTSREVALPYYEDKTDCIACGSCAYICPTGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEECCCEEEECCCCC
IKMEDGNGMRTITWPNCQVSFNLSAVPSAAPNGFPKNRSSIS
EEEECCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]