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The map label for this gene is nuoF2 [H]

Identifier: 57234506

GI number: 57234506

Start: 671735

End: 673606

Strand: Direct

Name: nuoF2 [H]

Synonym: DET0729

Alternate gene names: 57234506

Gene position: 671735-673606 (Clockwise)

Preceding gene: 57234507

Following gene: 57234505

Centisome position: 45.7

GC content: 48.29

Gene sequence:

>1872_bases
ATGATTAATACAGCAATTAAAAAGTTTAACAGTGTAGCCGAACTTGAGGCCAAACGGCAGGAAATCCTTAAAGACAGGAA
TTCCCAAAAGACGGTTATTGCTATTTGCTGCGGTACCGGCTGCCAGGCTTACGGGGCTAAAAAAGTTGCCGATGCCTTTG
AGGAAGAGCTTTCCAAAGCCGGTTTGGGAGATAAGGTTGAGGTCAGAACTACTGGCTGTCACGGCTTTTGCGAACGCGGC
CCGCTGGTAGTTATCCGCCCCCAGAATATTTTTTATCAGCGCCTGAAGATTTCAGATATACCAGAAATCATTGAAAAAAC
CATAAAAAACAATGAAGTTATTGAACGCCTGCTGTATGTTGACCCCATGACCAAAGACAAAAAGGTGCATGAACATGATG
TGCCTTTCTATAAAAAACAGATGCGTCTGGTTTTCGGCAACAACGGCAATATAGACCCTACCTCTATAGATGACTATCTG
GCGGTGGGCGGCTATAAATCCTTGGCCAAGGCTATATCTGGCCTGAAGCCGGAAGAAATTGTGGAAGAGGTTATCAAATC
CGGACTGCGCGGACGGGGCGGCGGCGGTTTCCCCACCGGCTGGAAATGGAAGTCCTGCCGGGACGCCCATGACCCGGTAA
AATATGTAATCTGCAACTGTGACGAGGGTGACCCGGGTGCGTTTATGGACCGCTCACTTATGGAGGGCAATCCCCATAGC
GTTATAGAAGGCATGATTATCGGTGCTTATGCTATTGGCTCTCATGAAGCTTATATTTATATCCGTGATGAATATCCTAT
GGCTGTAAAGCACGCCCTTTTGGCTATAAAACAGGCCGAAGACTATGGTCTGCTGGGTGAAAACATACTGGGCAGCGGCT
TTGACCTGAAAATCCATATCAACCGGGGTGGCGGTGCCTTTGTCTGCGGTGAGTCCACGGCCCTTATGGCCTCTCTGGAG
GGCAAGGTCGGCGAACCCCGTGCCAAGTATGTTCATACCTCTGAAAAGGGTCTGCATGACAAGCCTACCACCCTGAATAA
TGTGGAAACCTGGGCTAATATACCCCTGATTATAGAAAAAGGAGCAGACTGGTTTAACAAAATAGGTACAGAGGGCAGCA
AGGGAACCAAGATATTCTCACTGGTGGGTAAGGTAAACAATACCGGCCTGATTGAGGTTCCCATGGGTATTACCCTTCGG
GAAATTGTATATGATATAGGCGGCGGCATTCCCAAAGGCAAAAAATTCAAGGCTATTCAGACCGGCGGCCCTTCAGGCGG
CTGTCTGCCCGAAAGTATGCTGGATTTGCCGGTAGACTTTGATGAACTTACCAAGGCCGGTTCTATGATGGGTTCGGGTG
CCATGATTGTTATGGACGAAGATAACTGCATGGTGGAGATTGCCCGTTACTTCCTGAGCTTCCTTGAAGGAGAATCCTGC
GGCAAATGTGTACCCTGCCGTGAGGGTGTCAAGCGTATGCGCCAGATTCTGGAAAGAATAACCAAGGGCGAAGGCGAAGA
GGGAGATATAGAGCTTCTGGAGCAGCTTTCACAAGCGATACAGGACGCTTCTTTATGTGCTTTGGGCGGCAGTGCTCCTA
ATCCGGTTTTATCTACCATTAAATACTTCCGTGATGAGTACGAAGCCCATATCCGCCAGAAGACCTGTCCGGCTAAGGAA
TGTAAAGCCCTTATAACTTATAACATCATTGATGCCAATTGCCCCGGCTGCGGTTTATGTTACAAAGCCTGTCCGACAGG
GGCTATTATTTCACAGGGTAAAAAGATGCCGGTCATACTTGACCAGAGCAAATGTACTAAATGCGGTGCGTGTTTTGATG
CTTGTAAGCTCCACGCCGTAGAAATCAGATAA

Upstream 100 bases:

>100_bases
GTGGGCGCCTGTGCTTTGGGGCCTGTGGTGGTAGTGGACGGGCAGTATGTAGGCAATATGACTACTGAAAAGGTCAAACC
TTTGATTGAGGGATGCCAGG

Downstream 100 bases:

>100_bases
GGTAGGGTGGCTATGATAAATTTAACCATAGACGGCAAGAAATATCAGGCAGAAGAAGGTCAGACAATACTGGAAGTAGC
CAACCGGCATGGTATTTTTA

Product: [Fe] hydrogenase, HymB subunit, putative

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MINTAIKKFNSVAELEAKRQEILKDRNSQKTVIAICCGTGCQAYGAKKVADAFEEELSKAGLGDKVEVRTTGCHGFCERG
PLVVIRPQNIFYQRLKISDIPEIIEKTIKNNEVIERLLYVDPMTKDKKVHEHDVPFYKKQMRLVFGNNGNIDPTSIDDYL
AVGGYKSLAKAISGLKPEEIVEEVIKSGLRGRGGGGFPTGWKWKSCRDAHDPVKYVICNCDEGDPGAFMDRSLMEGNPHS
VIEGMIIGAYAIGSHEAYIYIRDEYPMAVKHALLAIKQAEDYGLLGENILGSGFDLKIHINRGGGAFVCGESTALMASLE
GKVGEPRAKYVHTSEKGLHDKPTTLNNVETWANIPLIIEKGADWFNKIGTEGSKGTKIFSLVGKVNNTGLIEVPMGITLR
EIVYDIGGGIPKGKKFKAIQTGGPSGGCLPESMLDLPVDFDELTKAGSMMGSGAMIVMDEDNCMVEIARYFLSFLEGESC
GKCVPCREGVKRMRQILERITKGEGEEGDIELLEQLSQAIQDASLCALGGSAPNPVLSTIKYFRDEYEAHIRQKTCPAKE
CKALITYNIIDANCPGCGLCYKACPTGAIISQGKKMPVILDQSKCTKCGACFDACKLHAVEIR

Sequences:

>Translated_623_residues
MINTAIKKFNSVAELEAKRQEILKDRNSQKTVIAICCGTGCQAYGAKKVADAFEEELSKAGLGDKVEVRTTGCHGFCERG
PLVVIRPQNIFYQRLKISDIPEIIEKTIKNNEVIERLLYVDPMTKDKKVHEHDVPFYKKQMRLVFGNNGNIDPTSIDDYL
AVGGYKSLAKAISGLKPEEIVEEVIKSGLRGRGGGGFPTGWKWKSCRDAHDPVKYVICNCDEGDPGAFMDRSLMEGNPHS
VIEGMIIGAYAIGSHEAYIYIRDEYPMAVKHALLAIKQAEDYGLLGENILGSGFDLKIHINRGGGAFVCGESTALMASLE
GKVGEPRAKYVHTSEKGLHDKPTTLNNVETWANIPLIIEKGADWFNKIGTEGSKGTKIFSLVGKVNNTGLIEVPMGITLR
EIVYDIGGGIPKGKKFKAIQTGGPSGGCLPESMLDLPVDFDELTKAGSMMGSGAMIVMDEDNCMVEIARYFLSFLEGESC
GKCVPCREGVKRMRQILERITKGEGEEGDIELLEQLSQAIQDASLCALGGSAPNPVLSTIKYFRDEYEAHIRQKTCPAKE
CKALITYNIIDANCPGCGLCYKACPTGAIISQGKKMPVILDQSKCTKCGACFDACKLHAVEIR
>Mature_623_residues
MINTAIKKFNSVAELEAKRQEILKDRNSQKTVIAICCGTGCQAYGAKKVADAFEEELSKAGLGDKVEVRTTGCHGFCERG
PLVVIRPQNIFYQRLKISDIPEIIEKTIKNNEVIERLLYVDPMTKDKKVHEHDVPFYKKQMRLVFGNNGNIDPTSIDDYL
AVGGYKSLAKAISGLKPEEIVEEVIKSGLRGRGGGGFPTGWKWKSCRDAHDPVKYVICNCDEGDPGAFMDRSLMEGNPHS
VIEGMIIGAYAIGSHEAYIYIRDEYPMAVKHALLAIKQAEDYGLLGENILGSGFDLKIHINRGGGAFVCGESTALMASLE
GKVGEPRAKYVHTSEKGLHDKPTTLNNVETWANIPLIIEKGADWFNKIGTEGSKGTKIFSLVGKVNNTGLIEVPMGITLR
EIVYDIGGGIPKGKKFKAIQTGGPSGGCLPESMLDLPVDFDELTKAGSMMGSGAMIVMDEDNCMVEIARYFLSFLEGESC
GKCVPCREGVKRMRQILERITKGEGEEGDIELLEQLSQAIQDASLCALGGSAPNPVLSTIKYFRDEYEAHIRQKTCPAKE
CKALITYNIIDANCPGCGLCYKACPTGAIISQGKKMPVILDQSKCTKCGACFDACKLHAVEIR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=380, Percent_Identity=39.7368421052632, Blast_Score=293, Evalue=4e-79,
Organism=Homo sapiens, GI260656005, Length=380, Percent_Identity=39.7368421052632, Blast_Score=293, Evalue=5e-79,
Organism=Escherichia coli, GI1788620, Length=404, Percent_Identity=43.5643564356436, Blast_Score=353, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI17531727, Length=378, Percent_Identity=41.2698412698413, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI19920794, Length=378, Percent_Identity=40.2116402116402, Blast_Score=291, Evalue=1e-78,
Organism=Drosophila melanogaster, GI45550452, Length=378, Percent_Identity=38.0952380952381, Blast_Score=271, Evalue=7e-73,
Organism=Drosophila melanogaster, GI24653819, Length=380, Percent_Identity=34.2105263157895, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24653817, Length=341, Percent_Identity=32.5513196480938, Blast_Score=197, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 67978; Mature: 67978

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINTAIKKFNSVAELEAKRQEILKDRNSQKTVIAICCGTGCQAYGAKKVADAFEEELSKA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHC
GLGDKVEVRTTGCHGFCERGPLVVIRPQNIFYQRLKISDIPEIIEKTIKNNEVIERLLYV
CCCCEEEEEECCCCCHHCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHC
DPMTKDKKVHEHDVPFYKKQMRLVFGNNGNIDPTSIDDYLAVGGYKSLAKAISGLKPEEI
CCCCCCCCCCCCCCCHHHHCEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCHHHH
VEEVIKSGLRGRGGGGFPTGWKWKSCRDAHDPVKYVICNCDEGDPGAFMDRSLMEGNPHS
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCHH
VIEGMIIGAYAIGSHEAYIYIRDEYPMAVKHALLAIKQAEDYGLLGENILGSGFDLKIHI
HHHHHEEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEEE
NRGGGAFVCGESTALMASLEGKVGEPRAKYVHTSEKGLHDKPTTLNNVETWANIPLIIEK
ECCCCEEEECCCCEEEEECCCCCCCCHHHEEECCCCCCCCCCCCCCCCHHHCCCCEEEEC
GADWFNKIGTEGSKGTKIFSLVGKVNNTGLIEVPMGITLREIVYDIGGGIPKGKKFKAIQ
CCHHHHHHCCCCCCCCCHHEEHHCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCEEEEE
TGGPSGGCLPESMLDLPVDFDELTKAGSMMGSGAMIVMDEDNCMVEIARYFLSFLEGESC
CCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCC
GKCVPCREGVKRMRQILERITKGEGEEGDIELLEQLSQAIQDASLCALGGSAPNPVLSTI
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHH
KYFRDEYEAHIRQKTCPAKECKALITYNIIDANCPGCGLCYKACPTGAIISQGKKMPVIL
HHHHHHHHHHHHHCCCCHHHCCEEEEEEEEECCCCCCCHHHHHCCCCHHHHCCCCCCEEE
DQSKCTKCGACFDACKLHAVEIR
CCCCCCHHHHHHHHHCEEEEEEC
>Mature Secondary Structure
MINTAIKKFNSVAELEAKRQEILKDRNSQKTVIAICCGTGCQAYGAKKVADAFEEELSKA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHC
GLGDKVEVRTTGCHGFCERGPLVVIRPQNIFYQRLKISDIPEIIEKTIKNNEVIERLLYV
CCCCEEEEEECCCCCHHCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHC
DPMTKDKKVHEHDVPFYKKQMRLVFGNNGNIDPTSIDDYLAVGGYKSLAKAISGLKPEEI
CCCCCCCCCCCCCCCHHHHCEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCHHHH
VEEVIKSGLRGRGGGGFPTGWKWKSCRDAHDPVKYVICNCDEGDPGAFMDRSLMEGNPHS
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCHH
VIEGMIIGAYAIGSHEAYIYIRDEYPMAVKHALLAIKQAEDYGLLGENILGSGFDLKIHI
HHHHHEEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEEE
NRGGGAFVCGESTALMASLEGKVGEPRAKYVHTSEKGLHDKPTTLNNVETWANIPLIIEK
ECCCCEEEECCCCEEEEECCCCCCCCHHHEEECCCCCCCCCCCCCCCCHHHCCCCEEEEC
GADWFNKIGTEGSKGTKIFSLVGKVNNTGLIEVPMGITLREIVYDIGGGIPKGKKFKAIQ
CCHHHHHHCCCCCCCCCHHEEHHCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCEEEEE
TGGPSGGCLPESMLDLPVDFDELTKAGSMMGSGAMIVMDEDNCMVEIARYFLSFLEGESC
CCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCC
GKCVPCREGVKRMRQILERITKGEGEEGDIELLEQLSQAIQDASLCALGGSAPNPVLSTI
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHH
KYFRDEYEAHIRQKTCPAKECKALITYNIIDANCPGCGLCYKACPTGAIISQGKKMPVIL
HHHHHHHHHHHHHCCCCHHHCCEEEEEEEEECCCCCCCHHHHHCCCCHHHHCCCCCCEEE
DQSKCTKCGACFDACKLHAVEIR
CCCCCCHHHHHHHHHCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]