Click here to switch to the map view.

The map label for this gene is panB

Identifier: 57234371

GI number: 57234371

Start: 737580

End: 738287

Strand: Direct

Name: panB

Synonym: DET0803

Alternate gene names: 57234371

Gene position: 737580-738287 (Clockwise)

Preceding gene: 57234380

Following gene: 57234370

Centisome position: 50.19

GC content: 49.15

Gene sequence:

>708_bases
ATGGTGGTGCTTGGGTATGATTCCACCGTAAGCGTGACCATGGAAGATATGCTGCACCATCTGAAAGCCGTAGTCAGGGG
CAGCCAAAAGGCCATGGTCATAGCCGATATGCCCTTTATGACCTATCACCTAAGCCCGGAACAGGCACTCCTGAACGCAG
GGCGTTTTATGCAGGAAGGCGGCGCTCAGGCAGTCAAACTGGAGGGCGGGGTAAATGTGGCTGACAAGGTTAAACGTATA
GTTGACTGCGGCATTCCCGTTATGGGGCATATAGGGCTGACTCCCCAGTCTGTTAACCAGCTAAGCGGGTTTAAAGTCCA
GGGGAAAACTCTGACAGCTGCTTTAGCCCTGCTAGAAGATGCCAGGGCTCTGGAAAAAGCCGGGGCGTTTGCTATAGTGC
TGGAGACTATGCCGGCTGAGCTGGCGGCCATGATTACCGCTGCCGTTTCCATACCTACCATAGGCATAGGGGCAGGTGAA
AATTGTGATGGCCAGGTGCAGGTGGTATCCGATATGCTGGGTATGTTTACTGATTTTGTACCTAAACATACCAAAAAATA
CGCTGATTTAAACGGTATTATCTCAAAAGCTGTATCCGGATATGTAACTGAGGTTGCAAAAGGTGAATTTCCCACTTTGA
AAGAAAGCTTCACCCTTGATAAAAAGGTATTGGAGGAACTGAAAAAGTGCGTATCATCAGAACAGTAG

Upstream 100 bases:

>100_bases
CAAGCAGAAGATAGCCATGCTGACTGCATATGACTATCCCACTGCCCAAATACTGGATAAAGCCGGTATACCGGCCATAC
TGGTGGGAGACAGTTTGGGT

Downstream 100 bases:

>100_bases
CCGAGATGCAAAAACTGCACCGTGAAATCTGCGGACCGGTTGGCCTGGTGCCGACTATGGGCTATCTGCACGAAGGGCAT
TTGTCACTGATTAGGGCTTC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MVVLGYDSTVSVTMEDMLHHLKAVVRGSQKAMVIADMPFMTYHLSPEQALLNAGRFMQEGGAQAVKLEGGVNVADKVKRI
VDCGIPVMGHIGLTPQSVNQLSGFKVQGKTLTAALALLEDARALEKAGAFAIVLETMPAELAAMITAAVSIPTIGIGAGE
NCDGQVQVVSDMLGMFTDFVPKHTKKYADLNGIISKAVSGYVTEVAKGEFPTLKESFTLDKKVLEELKKCVSSEQ

Sequences:

>Translated_235_residues
MVVLGYDSTVSVTMEDMLHHLKAVVRGSQKAMVIADMPFMTYHLSPEQALLNAGRFMQEGGAQAVKLEGGVNVADKVKRI
VDCGIPVMGHIGLTPQSVNQLSGFKVQGKTLTAALALLEDARALEKAGAFAIVLETMPAELAAMITAAVSIPTIGIGAGE
NCDGQVQVVSDMLGMFTDFVPKHTKKYADLNGIISKAVSGYVTEVAKGEFPTLKESFTLDKKVLEELKKCVSSEQ
>Mature_235_residues
MVVLGYDSTVSVTMEDMLHHLKAVVRGSQKAMVIADMPFMTYHLSPEQALLNAGRFMQEGGAQAVKLEGGVNVADKVKRI
VDCGIPVMGHIGLTPQSVNQLSGFKVQGKTLTAALALLEDARALEKAGAFAIVLETMPAELAAMITAAVSIPTIGIGAGE
NCDGQVQVVSDMLGMFTDFVPKHTKKYADLNGIISKAVSGYVTEVAKGEFPTLKESFTLDKKVLEELKKCVSSEQ

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=217, Percent_Identity=46.5437788018433, Blast_Score=183, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6319653, Length=234, Percent_Identity=40.1709401709402, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_DEHE1 (Q3Z8B4)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181535.1
- ProteinModelPortal:   Q3Z8B4
- SMR:   Q3Z8B4
- STRING:   Q3Z8B4
- GeneID:   3229838
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0803
- NMPDR:   fig|243164.3.peg.995
- TIGR:   DET0803
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- ProtClustDB:   PRK00311
- BioCyc:   DETH243164:DET_0803-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 25016; Mature: 25016

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: ACT_SITE 182-182 BINDING 83-83 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVLGYDSTVSVTMEDMLHHLKAVVRGSQKAMVIADMPFMTYHLSPEQALLNAGRFMQEG
CEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCHHHHHHHHHHHHHCC
GAQAVKLEGGVNVADKVKRIVDCGIPVMGHIGLTPQSVNQLSGFKVQGKTLTAALALLED
CCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEECCHHHHHHHHHHHH
ARALEKAGAFAIVLETMPAELAAMITAAVSIPTIGIGAGENCDGQVQVVSDMLGMFTDFV
HHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHH
PKHTKKYADLNGIISKAVSGYVTEVAKGEFPTLKESFTLDKKVLEELKKCVSSEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVVLGYDSTVSVTMEDMLHHLKAVVRGSQKAMVIADMPFMTYHLSPEQALLNAGRFMQEG
CEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCHHHHHHHHHHHHHCC
GAQAVKLEGGVNVADKVKRIVDCGIPVMGHIGLTPQSVNQLSGFKVQGKTLTAALALLED
CCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEECCHHHHHHHHHHHH
ARALEKAGAFAIVLETMPAELAAMITAAVSIPTIGIGAGENCDGQVQVVSDMLGMFTDFV
HHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHH
PKHTKKYADLNGIISKAVSGYVTEVAKGEFPTLKESFTLDKKVLEELKKCVSSEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA