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The map label for this gene is nuoI [C]

Identifier: 57234353

GI number: 57234353

Start: 796678

End: 797022

Strand: Direct

Name: nuoI [C]

Synonym: DET0868

Alternate gene names: 57234353

Gene position: 796678-797022 (Clockwise)

Preceding gene: 57234354

Following gene: 57234350

Centisome position: 54.21

GC content: 52.75

Gene sequence:

>345_bases
ATGCCCTGGATGATAGGAACAGTACTTAAAAATTTGTTTTCAACTCCCCCCACCCGCCGCTACCCATACGAAAAACGGGA
GTCATTTGCTGGTTCACGCGGTAACATCACCTGGGATGCCGGGCGTTGTGATATGTGCAACGATTGCGCCCGGGTCTGTC
CCGCCCGCGCTATTACTCTTAAACCGGAAAAACACCTTATAGAATATGACCCGCTAAAGTGTATTTACTGCGGTACTTGC
GCCGAAACCTGTCTTCAGCATGCAATCACCCAGCATCCGCTTTACGCCGCCCCGCAGGCTGAGCACGCTAGCCGGATAAC
CGAAGTCCACCACTACCGCTATTAG

Upstream 100 bases:

>100_bases
CCTGCTGGTCATGCTGCCGGGGTGTGAACTGGCAGATGTGCCTGTTATTACCTTATCTATAGACCCATGTATATCCTGCA
CGGAGCGTTAGGAGAAGGCT

Downstream 100 bases:

>100_bases
AAAAAGGTTTATCGCAGAGAAAAACCCCGGCTGTGACCGGGGTTTTATTATATCTATTATGATTCGCAGATATATTATGT
AATGTAATTAGGCCGTATCG

Product: hydrogenase, EchF subunit, putative

Products: NAD+; ubiquinol [C]

Alternate protein names: Ech Hydrogenase Subunit EchF; Hydrogenase EchF Subunit

Number of amino acids: Translated: 114; Mature: 113

Protein sequence:

>114_residues
MPWMIGTVLKNLFSTPPTRRYPYEKRESFAGSRGNITWDAGRCDMCNDCARVCPARAITLKPEKHLIEYDPLKCIYCGTC
AETCLQHAITQHPLYAAPQAEHASRITEVHHYRY

Sequences:

>Translated_114_residues
MPWMIGTVLKNLFSTPPTRRYPYEKRESFAGSRGNITWDAGRCDMCNDCARVCPARAITLKPEKHLIEYDPLKCIYCGTC
AETCLQHAITQHPLYAAPQAEHASRITEVHHYRY
>Mature_113_residues
PWMIGTVLKNLFSTPPTRRYPYEKRESFAGSRGNITWDAGRCDMCNDCARVCPARAITLKPEKHLIEYDPLKCIYCGTCA
ETCLQHAITQHPLYAAPQAEHASRITEVHHYRY

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 13087; Mature: 12956

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
9.6 %Cys+Met (Translated Protein)
7.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWMIGTVLKNLFSTPPTRRYPYEKRESFAGSRGNITWDAGRCDMCNDCARVCPARAITL
CCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEECCCCCCHHHHHHHHCCCCEEEE
KPEKHLIEYDPLKCIYCGTCAETCLQHAITQHPLYAAPQAEHASRITEVHHYRY
CCHHHCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PWMIGTVLKNLFSTPPTRRYPYEKRESFAGSRGNITWDAGRCDMCNDCARVCPARAITL
CCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCEEECCCCCCHHHHHHHHCCCCEEEE
KPEKHLIEYDPLKCIYCGTCAETCLQHAITQHPLYAAPQAEHASRITEVHHYRY
CCHHHCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA