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The map label for this gene is pilT-1 [H]

Identifier: 57234308

GI number: 57234308

Start: 844253

End: 845344

Strand: Direct

Name: pilT-1 [H]

Synonym: DET0922

Alternate gene names: 57234308

Gene position: 844253-845344 (Clockwise)

Preceding gene: 57234325

Following gene: 57234307

Centisome position: 57.44

GC content: 50.46

Gene sequence:

>1092_bases
ATGCGCGCAGACGAATTATTAAAAATAGCAGTAGAAAAAAAGGCTTCAGACCTCCACCTGAGAGTGCCGAACCCGCCTGT
ACTCAGAATAGACGGCGCCCTTAAACCCCAGACAGACTGGCCTGTTTTATACAACAAGGCCATTGAGCAGATACTGGAAG
AGATAACCACCCCCGAACAGCGTGAAATATTTTATGCCGAAAAAGAGCTGGACTTTGCCTACAGTGTAGCCGGGGTAGCC
CGCTTCAGGGTAAACGTGATGAAACAGCGCGGCTCTATCAGCATTGCCTTCCGCCAGGTGCCTTTTGAGATACGTTCTAT
AGATGAACTGGGTGTACCCAAAATCTGCAAAGAGCTTATCCTGAAACCGCGCGGCCTTATTCTGGTAACCGGGCCAACCG
GCAGCGGCAAATCCACCACCATGGCCGCCATGGTAGACCACCTGAATAATACTGATTCGCGTAACGTCATTACCATAGAA
GACCCCATTGAGTATCTTTATACCAATAACCGCTGCATTATTGCCCAGAGAGATTTGGGTGACGATACCAAGTCTTTTTC
CACTGCCCTGAAATATGCCCTCCGGCATGACCCTGACGTAATACTGGTGGGTGAAATGCGTGACCTGGAGACTATTTCCA
CCGCCATCAGCGCCGCCGAAACAGGCCATCTGGTAGTGGGTACTTTGCACACTACCGATGCCCCCCAGACTATAGACCGC
TTAATAGATATATTCCCTCCTGACCAGCAGCAGCAGATACGCATGCAGCTGTCTCTGGTTATTGAGGCGGTACTGGTACA
GACGCTGCTGCCAAAACGGGAAGGCAAAGGGCGGGTGGCCGCTTTTGAAATAATGGTAGCCAATACCGCCGTACGCAACC
TGATACGTGACAAAAAGGCCCATGAACTGCATAACGTAATGCAGCTGAGTGCCAAAGACGGTATGCAGACCCTTGACCAG
TCTTTGGCAGGTCTGGTACGCCAGAATATAGTTTCGCTGGAAGAAGCCCTGTTAAAAAGCAGTAACCCGGAAAGACTGCA
AAAATTGCTGGCATACCAGCAAACCCAGCAGACCAAGCCCCACCAGTTTTAA

Upstream 100 bases:

>100_bases
TATAATATAATAAACTTAAATAGTACTTTTGGGGGATTTTCCTTACTAAAACTAACTTATACTATAATATGATATCATAT
CCGGTTCAGAAGGAAGACAA

Downstream 100 bases:

>100_bases
ACTGCCGGCAACACGCAGTACCATTTGCCGTATTAAATGCAGCAAGTTCCGGCGGGGTTTGCCCCGGTGCACTCTGCAAA
TATATTTCTTTTCCGCACTT

Product: twitching mobility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MRADELLKIAVEKKASDLHLRVPNPPVLRIDGALKPQTDWPVLYNKAIEQILEEITTPEQREIFYAEKELDFAYSVAGVA
RFRVNVMKQRGSISIAFRQVPFEIRSIDELGVPKICKELILKPRGLILVTGPTGSGKSTTMAAMVDHLNNTDSRNVITIE
DPIEYLYTNNRCIIAQRDLGDDTKSFSTALKYALRHDPDVILVGEMRDLETISTAISAAETGHLVVGTLHTTDAPQTIDR
LIDIFPPDQQQQIRMQLSLVIEAVLVQTLLPKREGKGRVAAFEIMVANTAVRNLIRDKKAHELHNVMQLSAKDGMQTLDQ
SLAGLVRQNIVSLEEALLKSSNPERLQKLLAYQQTQQTKPHQF

Sequences:

>Translated_363_residues
MRADELLKIAVEKKASDLHLRVPNPPVLRIDGALKPQTDWPVLYNKAIEQILEEITTPEQREIFYAEKELDFAYSVAGVA
RFRVNVMKQRGSISIAFRQVPFEIRSIDELGVPKICKELILKPRGLILVTGPTGSGKSTTMAAMVDHLNNTDSRNVITIE
DPIEYLYTNNRCIIAQRDLGDDTKSFSTALKYALRHDPDVILVGEMRDLETISTAISAAETGHLVVGTLHTTDAPQTIDR
LIDIFPPDQQQQIRMQLSLVIEAVLVQTLLPKREGKGRVAAFEIMVANTAVRNLIRDKKAHELHNVMQLSAKDGMQTLDQ
SLAGLVRQNIVSLEEALLKSSNPERLQKLLAYQQTQQTKPHQF
>Mature_363_residues
MRADELLKIAVEKKASDLHLRVPNPPVLRIDGALKPQTDWPVLYNKAIEQILEEITTPEQREIFYAEKELDFAYSVAGVA
RFRVNVMKQRGSISIAFRQVPFEIRSIDELGVPKICKELILKPRGLILVTGPTGSGKSTTMAAMVDHLNNTDSRNVITIE
DPIEYLYTNNRCIIAQRDLGDDTKSFSTALKYALRHDPDVILVGEMRDLETISTAISAAETGHLVVGTLHTTDAPQTIDR
LIDIFPPDQQQQIRMQLSLVIEAVLVQTLLPKREGKGRVAAFEIMVANTAVRNLIRDKKAHELHNVMQLSAKDGMQTLDQ
SLAGLVRQNIVSLEEALLKSSNPERLQKLLAYQQTQQTKPHQF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=322, Percent_Identity=44.4099378881988, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1786296, Length=378, Percent_Identity=30.4232804232804, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789723, Length=144, Percent_Identity=46.5277777777778, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40716; Mature: 40716

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRADELLKIAVEKKASDLHLRVPNPPVLRIDGALKPQTDWPVLYNKAIEQILEEITTPEQ
CCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCH
REIFYAEKELDFAYSVAGVARFRVNVMKQRGSISIAFRQVPFEIRSIDELGVPKICKELI
HHEEEEHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCCCCCHHHHHHHHH
LKPRGLILVTGPTGSGKSTTMAAMVDHLNNTDSRNVITIEDPIEYLYTNNRCIIAQRDLG
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHEEECCEEEEEECCCC
DDTKSFSTALKYALRHDPDVILVGEMRDLETISTAISAAETGHLVVGTLHTTDAPQTIDR
CCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
LIDIFPPDQQQQIRMQLSLVIEAVLVQTLLPKREGKGRVAAFEIMVANTAVRNLIRDKKA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHH
HELHNVMQLSAKDGMQTLDQSLAGLVRQNIVSLEEALLKSSNPERLQKLLAYQQTQQTKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
HQF
CCC
>Mature Secondary Structure
MRADELLKIAVEKKASDLHLRVPNPPVLRIDGALKPQTDWPVLYNKAIEQILEEITTPEQ
CCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCH
REIFYAEKELDFAYSVAGVARFRVNVMKQRGSISIAFRQVPFEIRSIDELGVPKICKELI
HHEEEEHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCCCCCHHHHHHHHH
LKPRGLILVTGPTGSGKSTTMAAMVDHLNNTDSRNVITIEDPIEYLYTNNRCIIAQRDLG
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHEEECCEEEEEECCCC
DDTKSFSTALKYALRHDPDVILVGEMRDLETISTAISAAETGHLVVGTLHTTDAPQTIDR
CCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
LIDIFPPDQQQQIRMQLSLVIEAVLVQTLLPKREGKGRVAAFEIMVANTAVRNLIRDKKA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHH
HELHNVMQLSAKDGMQTLDQSLAGLVRQNIVSLEEALLKSSNPERLQKLLAYQQTQQTKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
HQF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]