Click here to switch to the map view.

The map label for this gene is hemK [H]

Identifier: 57234022

GI number: 57234022

Start: 1110472

End: 1111305

Strand: Direct

Name: hemK [H]

Synonym: DET1211

Alternate gene names: 57234022

Gene position: 1110472-1111305 (Clockwise)

Preceding gene: 57234023

Following gene: 57234009

Centisome position: 75.56

GC content: 54.44

Gene sequence:

>834_bases
ATGAAATACCGGGAGGCATGGCAGAAAGCCGCCGGTCTCCTTGAGGACAGTGGGCTGGAAGAAGCCCGTCTGGAAGCTGA
AATTCTCCTGCGGCATACTCTGGGCATCAGCCGGGTGCAGCTTCATCTGGAACTGGAACGGGAACTACACCCTGATAAAG
AAGCCGCTTATTTCCAAACCCTGAAGCGCCGCCTTGAGGGTGAGCCCAGTGCCTATATCACAGGTGCAAAAGAGTTTTAC
GGCCGCGCCTTTCAGGTGGATAAACGGGTGCTGATACCCAGGCCTGAAACCGAACACCTTATAGAAAAAGCCCTTCGGAT
TGCCCGCAGTTATGAAAGCCCCTATATTGCAGATATCGGCACAGGCAGCGGGGCTATTGCCATAACGCTGGCACTGGAGC
TGAAAGATGCTTATGTATATGCCACTGACATATCCGCAGAGGCTCTGGAAGTAGCCCGGAATAACGCCGCTGAATACCGG
CTGGAAAAGCGCCTCATGTTTTACCGGGGAGACCTGCTGGAAAGCCTGCCCGAAATGGTAGATATACTAATGGCAAACCT
GCCCTATGTACCCACCGCCGAAGCCGGGCTGCTTAAGGGCGAACCTGTCTCAGCCCTGGACGGTGGGGCAGACGGGTTGG
ATATATACCGCCGCTTGATACCTGTTTTACCCGGGAAACTTCGCCCCGGCGGCACAGCCCTGCTGGAAATAGGCATACAC
CAGTCAGAAGTACTTGCCAACTATATAAAAGAGTACCTGCCTCAAGCGGCAGTTGAAATAAGCCCTGATTATGCCGGCAT
ACCCAGAGTAGCCGGTATAACCCTGCCCGCCTGA

Upstream 100 bases:

>100_bases
CTGCCCATAACCTGCCCCACATACTGGAAGGATATTTAGACGAAATTATTGATACTTTGGCTACTCACGAACAGACCGAA
CTTCTAAAGGGCGAAGATTA

Downstream 100 bases:

>100_bases
AAACTGTACTTCTAACATATTCCGCTAAATAAAAAGAGGGGCTTTCGCCCCCCTTGAAAAAGCACCTAGCCCTGATAAAC
TAAACTTTTTCTGCTTCCAG

Product: HemK family modification methylase

Products: NA

Alternate protein names: HemK protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKYREAWQKAAGLLEDSGLEEARLEAEILLRHTLGISRVQLHLELERELHPDKEAAYFQTLKRRLEGEPSAYITGAKEFY
GRAFQVDKRVLIPRPETEHLIEKALRIARSYESPYIADIGTGSGAIAITLALELKDAYVYATDISAEALEVARNNAAEYR
LEKRLMFYRGDLLESLPEMVDILMANLPYVPTAEAGLLKGEPVSALDGGADGLDIYRRLIPVLPGKLRPGGTALLEIGIH
QSEVLANYIKEYLPQAAVEISPDYAGIPRVAGITLPA

Sequences:

>Translated_277_residues
MKYREAWQKAAGLLEDSGLEEARLEAEILLRHTLGISRVQLHLELERELHPDKEAAYFQTLKRRLEGEPSAYITGAKEFY
GRAFQVDKRVLIPRPETEHLIEKALRIARSYESPYIADIGTGSGAIAITLALELKDAYVYATDISAEALEVARNNAAEYR
LEKRLMFYRGDLLESLPEMVDILMANLPYVPTAEAGLLKGEPVSALDGGADGLDIYRRLIPVLPGKLRPGGTALLEIGIH
QSEVLANYIKEYLPQAAVEISPDYAGIPRVAGITLPA
>Mature_277_residues
MKYREAWQKAAGLLEDSGLEEARLEAEILLRHTLGISRVQLHLELERELHPDKEAAYFQTLKRRLEGEPSAYITGAKEFY
GRAFQVDKRVLIPRPETEHLIEKALRIARSYESPYIADIGTGSGAIAITLALELKDAYVYATDISAEALEVARNNAAEYR
LEKRLMFYRGDLLESLPEMVDILMANLPYVPTAEAGLLKGEPVSALDGGADGLDIYRRLIPVLPGKLRPGGTALLEIGIH
QSEVLANYIKEYLPQAAVEISPDYAGIPRVAGITLPA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=287, Percent_Identity=31.3588850174216, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1787463, Length=283, Percent_Identity=33.5689045936396, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI87082085, Length=195, Percent_Identity=34.8717948717949, Blast_Score=102, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24582226, Length=238, Percent_Identity=28.1512605042017, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 30668; Mature: 30668

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYREAWQKAAGLLEDSGLEEARLEAEILLRHTLGISRVQLHLELERELHPDKEAAYFQT
CCHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEHHHCCCCHHHHHHHH
LKRRLEGEPSAYITGAKEFYGRAFQVDKRVLIPRPETEHLIEKALRIARSYESPYIADIG
HHHHHCCCCCEEEECCHHHCCCHHCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEC
TGSGAIAITLALELKDAYVYATDISAEALEVARNNAAEYRLEKRLMFYRGDLLESLPEMV
CCCCEEEEEEEEEECCEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
DILMANLPYVPTAEAGLLKGEPVSALDGGADGLDIYRRLIPVLPGKLRPGGTALLEIGIH
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCC
QSEVLANYIKEYLPQAAVEISPDYAGIPRVAGITLPA
HHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCEECCC
>Mature Secondary Structure
MKYREAWQKAAGLLEDSGLEEARLEAEILLRHTLGISRVQLHLELERELHPDKEAAYFQT
CCHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEHHHCCCCHHHHHHHH
LKRRLEGEPSAYITGAKEFYGRAFQVDKRVLIPRPETEHLIEKALRIARSYESPYIADIG
HHHHHCCCCCEEEECCHHHCCCHHCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEC
TGSGAIAITLALELKDAYVYATDISAEALEVARNNAAEYRLEKRLMFYRGDLLESLPEMV
CCCCEEEEEEEEEECCEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
DILMANLPYVPTAEAGLLKGEPVSALDGGADGLDIYRRLIPVLPGKLRPGGTALLEIGIH
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCC
QSEVLANYIKEYLPQAAVEISPDYAGIPRVAGITLPA
HHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA