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The map label for this gene is prfA

Identifier: 57234023

GI number: 57234023

Start: 1109405

End: 1110472

Strand: Direct

Name: prfA

Synonym: DET1210

Alternate gene names: 57234023

Gene position: 1109405-1110472 (Clockwise)

Preceding gene: 57234024

Following gene: 57234022

Centisome position: 75.48

GC content: 50.66

Gene sequence:

>1068_bases
ATGCTTGAAAGATTAGAAAACTGCGAAAAACGCTTCCGGGAAATTGAAGAGGAAATAAGCAAACCGGAAGTTATAAATGA
TGCCCGGCTGGTGCGTACTCTGGCCCAGGAGCGGGCTGATTTGCAGACCAAAGTGGAAATGTACCGCCGCTATAAAGCCA
TATCCAAAGAGCTTGAAGATACCAAAAACCTGCTTGAGATTGAAAAAGACGAAGACATGAAGGACATGGTACGCGGTGAG
ATTGCCAGCCTTGAAAAATCTATGGCAGACCTCTACACCCAGATGACCCTTGAGCTTTTGCCCAAAGACCCCAATGATGA
CAAAAGCATTATCATGGAAATACGGGCAGGCACAGGCGGTGACGAAGCCGGGCTGTTTGCCGCAGACCTGTATAAGATGT
ATACCCGCTATGCCCTGCTGAAAAACTGGAAAACCGAAGTCATTGACATAAACGGCAACGTAGCCGGCATTATCAAAGAA
GTAGTCTTTGAGGTAAACGGCAAAGGGGCTTTCAGCCGCTTGAAGTATGAACGGGGTGTCCACCGGGTACAGCGTGTCCC
CCAGACCGAGGCTTCCGGCCGTATCCATACCTCTACCGCCACTGTCGCCGTCCTCCCCCAGGTAGAGGAAGTAGACATAG
ATATAAATATGGACGATGTAAGGGTGGATATATTTCATTCCAGCGGCGCCGGCGGACAGAACGTCCAGAAAGTGGCTACT
GCCATCCGCCTGACCCATATACCCACGGGCTTGGTTGTCTGCTGTCAGGACGAACGCTCACAGCTGAAAAACAAGAACAA
GGCCTTTGCGGTGCTGCGTGCCAGACTGATGGAACTGGAACAAAGCAAGGTGGACGAAGAACGCACTGAAAGCCGCCGCG
CCCAGGTGGGACAGGCAGACCGCAGTGAAAAAATACGCACTTACAATTTCCCCCAGGACCGCCTGACAGACCACCGGATA
GGGCTTACTGCCCATAACCTGCCCCACATACTGGAAGGATATTTAGACGAAATTATTGATACTTTGGCTACTCACGAACA
GACCGAACTTCTAAAGGGCGAAGATTAA

Upstream 100 bases:

>100_bases
TCCGGCGGGTACAGTTTCGGCTGTACCCGCTCTCTTTTGTAAAATTTTTGGCTATAGGCTAATATTTTAACTGTCATCAA
AGAAATAAGGGTTTGGGACA

Downstream 100 bases:

>100_bases
TGAAATACCGGGAGGCATGGCAGAAAGCCGCCGGTCTCCTTGAGGACAGTGGGCTGGAAGAAGCCCGTCTGGAAGCTGAA
ATTCTCCTGCGGCATACTCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MLERLENCEKRFREIEEEISKPEVINDARLVRTLAQERADLQTKVEMYRRYKAISKELEDTKNLLEIEKDEDMKDMVRGE
IASLEKSMADLYTQMTLELLPKDPNDDKSIIMEIRAGTGGDEAGLFAADLYKMYTRYALLKNWKTEVIDINGNVAGIIKE
VVFEVNGKGAFSRLKYERGVHRVQRVPQTEASGRIHTSTATVAVLPQVEEVDIDINMDDVRVDIFHSSGAGGQNVQKVAT
AIRLTHIPTGLVVCCQDERSQLKNKNKAFAVLRARLMELEQSKVDEERTESRRAQVGQADRSEKIRTYNFPQDRLTDHRI
GLTAHNLPHILEGYLDEIIDTLATHEQTELLKGED

Sequences:

>Translated_355_residues
MLERLENCEKRFREIEEEISKPEVINDARLVRTLAQERADLQTKVEMYRRYKAISKELEDTKNLLEIEKDEDMKDMVRGE
IASLEKSMADLYTQMTLELLPKDPNDDKSIIMEIRAGTGGDEAGLFAADLYKMYTRYALLKNWKTEVIDINGNVAGIIKE
VVFEVNGKGAFSRLKYERGVHRVQRVPQTEASGRIHTSTATVAVLPQVEEVDIDINMDDVRVDIFHSSGAGGQNVQKVAT
AIRLTHIPTGLVVCCQDERSQLKNKNKAFAVLRARLMELEQSKVDEERTESRRAQVGQADRSEKIRTYNFPQDRLTDHRI
GLTAHNLPHILEGYLDEIIDTLATHEQTELLKGED
>Mature_355_residues
MLERLENCEKRFREIEEEISKPEVINDARLVRTLAQERADLQTKVEMYRRYKAISKELEDTKNLLEIEKDEDMKDMVRGE
IASLEKSMADLYTQMTLELLPKDPNDDKSIIMEIRAGTGGDEAGLFAADLYKMYTRYALLKNWKTEVIDINGNVAGIIKE
VVFEVNGKGAFSRLKYERGVHRVQRVPQTEASGRIHTSTATVAVLPQVEEVDIDINMDDVRVDIFHSSGAGGQNVQKVAT
AIRLTHIPTGLVVCCQDERSQLKNKNKAFAVLRARLMELEQSKVDEERTESRRAQVGQADRSEKIRTYNFPQDRLTDHRI
GLTAHNLPHILEGYLDEIIDTLATHEQTELLKGED

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=298, Percent_Identity=42.9530201342282, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI34577120, Length=348, Percent_Identity=36.7816091954023, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI166795305, Length=194, Percent_Identity=40.7216494845361, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=42.6136363636364, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=35.693215339233, Blast_Score=205, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17542784, Length=325, Percent_Identity=34.4615384615385, Blast_Score=181, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=325, Percent_Identity=42.4615384615385, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI19921226, Length=318, Percent_Identity=40.5660377358491, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_DEHE1 (Q3Z776)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181923.1
- HSSP:   P0A7I0
- ProteinModelPortal:   Q3Z776
- SMR:   Q3Z776
- STRING:   Q3Z776
- GeneID:   3229489
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET1210
- NMPDR:   fig|243164.3.peg.1112
- TIGR:   DET1210
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q3Z776
- ProtClustDB:   PRK00591
- BioCyc:   DETH243164:DET_1210-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40500; Mature: 40500

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERLENCEKRFREIEEEISKPEVINDARLVRTLAQERADLQTKVEMYRRYKAISKELED
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKNLLEIEKDEDMKDMVRGEIASLEKSMADLYTQMTLELLPKDPNDDKSIIMEIRAGTGG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCC
DEAGLFAADLYKMYTRYALLKNWKTEVIDINGNVAGIIKEVVFEVNGKGAFSRLKYERGV
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
HRVQRVPQTEASGRIHTSTATVAVLPQVEEVDIDINMDDVRVDIFHSSGAGGQNVQKVAT
HHHHHCCCCCCCCEEEECCEEEEECCCHHHEEEEECCCCEEEEEEECCCCCCCHHHHHHH
AIRLTHIPTGLVVCCQDERSQLKNKNKAFAVLRARLMELEQSKVDEERTESRRAQVGQAD
HHHHHCCCCCEEEEECHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
RSEKIRTYNFPQDRLTDHRIGLTAHNLPHILEGYLDEIIDTLATHEQTELLKGED
CCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCC
>Mature Secondary Structure
MLERLENCEKRFREIEEEISKPEVINDARLVRTLAQERADLQTKVEMYRRYKAISKELED
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKNLLEIEKDEDMKDMVRGEIASLEKSMADLYTQMTLELLPKDPNDDKSIIMEIRAGTGG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCC
DEAGLFAADLYKMYTRYALLKNWKTEVIDINGNVAGIIKEVVFEVNGKGAFSRLKYERGV
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
HRVQRVPQTEASGRIHTSTATVAVLPQVEEVDIDINMDDVRVDIFHSSGAGGQNVQKVAT
HHHHHCCCCCCCCEEEECCEEEEECCCHHHEEEEECCCCEEEEEEECCCCCCCHHHHHHH
AIRLTHIPTGLVVCCQDERSQLKNKNKAFAVLRARLMELEQSKVDEERTESRRAQVGQAD
HHHHHCCCCCEEEEECHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
RSEKIRTYNFPQDRLTDHRIGLTAHNLPHILEGYLDEIIDTLATHEQTELLKGED
CCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA