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The map label for this gene is pilT-2 [H]

Identifier: 57233979

GI number: 57233979

Start: 1141429

End: 1142505

Strand: Direct

Name: pilT-2 [H]

Synonym: DET1255

Alternate gene names: 57233979

Gene position: 1141429-1142505 (Clockwise)

Preceding gene: 57233981

Following gene: 57233978

Centisome position: 77.66

GC content: 52.18

Gene sequence:

>1077_bases
TTGATAGAGGTATATGAACTGCTGAAACTGGTAGTAGACAACGACGCCTCAGATTTACATATCAAAGCCCCTACCGCACC
GGTACTGCGCATAAACGGGGACCTAATACGCCAGATGCAATACCCGCCAATTACTCCGGAAGATGCCGAACAAATTTTCA
CCGCTATTACTACCCCCCACCAGCAGGAGATATTTGCCGCCGAAAATGAACTGGATTTCGTCTATTCGGTTACCAATCTG
GCCCGCTTCCGGGTCAATGTACTCCGCCAGAGGGGCAGCCTGAGTCTGGCTTTCAGGGTAGTCCCCTTTGCTATCCGCAA
CATAGATGAACTTAAGCTGCCGGTTATCCTTAAAAACCTGATTATCCGCCCGCGCGGGTTGCTGCTGGTTACCGGCCCTA
CCGGCAGCGGCAAATCCACCACCCAGGCCGCCATGATAGAGCACCTGAACAACTGCCGCCGGTGCAACGTGATTACTATT
GAAGACCCCATAGAGTACCTGTTCCATGACAACGAGTGTATCATATCCCAGCGTGAGCTTGGGGGAGATACCCATTCCTT
TGCAACGGCCTTAAAACACGCCCTCCGCCATGACCCCAACGTAATAGTCATAGGCGAAATGAGAGATTTGGAAACCATAT
CCACTGCCATTGCCGCCGCCGAAACAGGCCATCTGGTAATAGGCACCCTCCACACTACCGATGCCCCCCAGACGGTTGAC
CGGCTGATAGATATGTTCCCGCCCTCCCAGCAGCAGCAGATACGCCTCCAGCTTTCCCAGGTACTGGAAGCCATTATCTC
CCAGACCCTTATTCCCAGACTGGATAATAAAGGCCGGGTGGCCGCTTTTGAAATATTGATTGCCACCAATGCCGCCCGCA
ACCTTATCCGCGAAGGTAAAACGTTTGAACTGCTGACCATAATGCAACTGGGCAAAAATGAAGGTATGCAAACACTGGAT
AATGAACTGACCCGCCTGGCAAAAGACGGGGTAATAAGCACCGAAAACGCTGTTTTGAAAAGCAGTAACCCCGAAAGACT
GCGTAAGACCCTGTGCGGTGTGAACCGGGGGACTTAG

Upstream 100 bases:

>100_bases
ACTTCTCTTGACATATGTGTATTATAGGTTATTATACATATAATAATTTTAAATATTATCAGCCTGAGTAATATCTGTCT
GCAGCTTGGGAGGTGCGACC

Downstream 100 bases:

>100_bases
AAACCCTGCTGACAGGCGGGTAAATTTCACCTTGTCCAGGTGCCTTTTTAAATTGCCCTCCGGCTTGGGAAGTGGTATAT
TCTAGTGCTATGAAAAGCAG

Product: twitching mobility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MIEVYELLKLVVDNDASDLHIKAPTAPVLRINGDLIRQMQYPPITPEDAEQIFTAITTPHQQEIFAAENELDFVYSVTNL
ARFRVNVLRQRGSLSLAFRVVPFAIRNIDELKLPVILKNLIIRPRGLLLVTGPTGSGKSTTQAAMIEHLNNCRRCNVITI
EDPIEYLFHDNECIISQRELGGDTHSFATALKHALRHDPNVIVIGEMRDLETISTAIAAAETGHLVIGTLHTTDAPQTVD
RLIDMFPPSQQQQIRLQLSQVLEAIISQTLIPRLDNKGRVAAFEILIATNAARNLIREGKTFELLTIMQLGKNEGMQTLD
NELTRLAKDGVISTENAVLKSSNPERLRKTLCGVNRGT

Sequences:

>Translated_358_residues
MIEVYELLKLVVDNDASDLHIKAPTAPVLRINGDLIRQMQYPPITPEDAEQIFTAITTPHQQEIFAAENELDFVYSVTNL
ARFRVNVLRQRGSLSLAFRVVPFAIRNIDELKLPVILKNLIIRPRGLLLVTGPTGSGKSTTQAAMIEHLNNCRRCNVITI
EDPIEYLFHDNECIISQRELGGDTHSFATALKHALRHDPNVIVIGEMRDLETISTAIAAAETGHLVIGTLHTTDAPQTVD
RLIDMFPPSQQQQIRLQLSQVLEAIISQTLIPRLDNKGRVAAFEILIATNAARNLIREGKTFELLTIMQLGKNEGMQTLD
NELTRLAKDGVISTENAVLKSSNPERLRKTLCGVNRGT
>Mature_358_residues
MIEVYELLKLVVDNDASDLHIKAPTAPVLRINGDLIRQMQYPPITPEDAEQIFTAITTPHQQEIFAAENELDFVYSVTNL
ARFRVNVLRQRGSLSLAFRVVPFAIRNIDELKLPVILKNLIIRPRGLLLVTGPTGSGKSTTQAAMIEHLNNCRRCNVITI
EDPIEYLFHDNECIISQRELGGDTHSFATALKHALRHDPNVIVIGEMRDLETISTAIAAAETGHLVIGTLHTTDAPQTVD
RLIDMFPPSQQQQIRLQLSQVLEAIISQTLIPRLDNKGRVAAFEILIATNAARNLIREGKTFELLTIMQLGKNEGMQTLD
NELTRLAKDGVISTENAVLKSSNPERLRKTLCGVNRGT

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=326, Percent_Identity=42.0245398773006, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1789723, Length=159, Percent_Identity=46.5408805031447, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786296, Length=363, Percent_Identity=31.404958677686, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39858; Mature: 39858

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVYELLKLVVDNDASDLHIKAPTAPVLRINGDLIRQMQYPPITPEDAEQIFTAITTPH
CCCHHHHHHHHHCCCCCCEEEECCCCCEEEECHHHHHHCCCCCCCCCHHHHHHHHHCCCC
QQEIFAAENELDFVYSVTNLARFRVNVLRQRGSLSLAFRVVPFAIRNIDELKLPVILKNL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCHHHHHHHHHHHHH
IIRPRGLLLVTGPTGSGKSTTQAAMIEHLNNCRRCNVITIEDPIEYLFHDNECIISQREL
HCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEECHHHHHCCCCCEEEEHHHC
GGDTHSFATALKHALRHDPNVIVIGEMRDLETISTAIAAAETGHLVIGTLHTTDAPQTVD
CCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHH
RLIDMFPPSQQQQIRLQLSQVLEAIISQTLIPRLDNKGRVAAFEILIATNAARNLIREGK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHHHCCC
TFELLTIMQLGKNEGMQTLDNELTRLAKDGVISTENAVLKSSNPERLRKTLCGVNRGT
CEEEHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIEVYELLKLVVDNDASDLHIKAPTAPVLRINGDLIRQMQYPPITPEDAEQIFTAITTPH
CCCHHHHHHHHHCCCCCCEEEECCCCCEEEECHHHHHHCCCCCCCCCHHHHHHHHHCCCC
QQEIFAAENELDFVYSVTNLARFRVNVLRQRGSLSLAFRVVPFAIRNIDELKLPVILKNL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCHHHHHHHHHHHHH
IIRPRGLLLVTGPTGSGKSTTQAAMIEHLNNCRRCNVITIEDPIEYLFHDNECIISQREL
HCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEECHHHHHCCCCCEEEEHHHC
GGDTHSFATALKHALRHDPNVIVIGEMRDLETISTAIAAAETGHLVIGTLHTTDAPQTVD
CCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHH
RLIDMFPPSQQQQIRLQLSQVLEAIISQTLIPRLDNKGRVAAFEILIATNAARNLIREGK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHHHCCC
TFELLTIMQLGKNEGMQTLDNELTRLAKDGVISTENAVLKSSNPERLRKTLCGVNRGT
CEEEHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]