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The map label for this gene is proA

Identifier: 57233915

GI number: 57233915

Start: 1175125

End: 1176399

Strand: Direct

Name: proA

Synonym: DET1288

Alternate gene names: 57233915

Gene position: 1175125-1176399 (Clockwise)

Preceding gene: 57233919

Following gene: 57235102

Centisome position: 79.96

GC content: 52.39

Gene sequence:

>1275_bases
ATGGAAAAAGCACTGCTGGAAATAGAAGAAAAAGCCCGTCTGGCCAAAGCCGCTTCCCGCCCTTTAAGCTATGCTTCATC
CGCCCAAAAAGATGCCGCCCTTAAAAATATTGCCGCCTGCCTGCTTAATAACGCCCCGGCCATACTGGAAGCTAATTTGA
AAGACCAGACCGAAGCTAAAGCGGCGGGTATGACTGCTGCCATGCTGGACCGCCTGATTATTACCCAGAGCCGGCTGGAA
GGGATTGCCAAAGATACGCTGGCTATTGCCGCCCTGCCTGACCCGGTGGGTGAAATATTTGATATGAACACTATGCCTAA
CGGACTGGTGATAGGTAAAAAAAGAGTGCCGCTGGGTGTTATTGCGGCTATTTATGAAAGCCGCCCCAATGTCACGGTGG
ATATTGCCGCTTTGTGCCTGAAAGCCGGGAATGCGGTTATCCTGCGCGGCGGCAAGGAAACTATCCACTCAAACACCATT
CTGGCAAGGCTGATACGCCAGGCGGTTGAAGAGGCAGGCCTGCCCCAAGAAGCTGTCCAGTTTATAGAAAATACTGAGCA
TAGTCTGGTAAACCACCTGCTCAAACTTTCAGACCAGATAGATTTGGTCATACCCAGAGGCGGCGCCGGGCTGATTAGTT
ATGTTAAACAAAACTCGTTTATACCGGTGGTTGCCGGCGGCATAGGGGTAGTCCACATATACGTGGATGCAGATGCCAAT
ATAGCTGATGCGGTAAACATAGCTTACAATTCCAAAGTACAGCGCCCCACTGTCTGCAACGCCATGGATACCCTGCTGGT
ACACAAAGATATAGCCGCCGCTTTCCTGCCGGTGGTTGCCGCCGAATGGAGCAAAGCCGGAGTGGAAATACGGGCAGATA
AAACGGCTATGGAAATACTGGAAAATGCCTCCAGCTGCAAGTTGATACCCGCCGCCGCAGATGACTGGGGCAAGGAATTT
CTGGCACTGGTTGCCGCCGTAAAGGTAGTAGACAGCTTGGACGAAGCTCTTTCGCATATTGCCAGATACGGCTCAGGGCA
TACCGAAAGCATAGTCACCCAGAACTATACCTCTTCCCAGCGTTTTCTGAACGAAGTGGATGCGGCTGCCGTTATGGTAA
ATGCCTCCACCCGTTTTACAGACGGTTCGCAGTTCGGGCTGGGAGCGGAACTGGGCATAAGCACCCAGAAGATGCACGCC
CGCGGCCCCATGGGGCTTAAGGAGATAACCAGCTACAAATGGATAGTCTACGGGAACGGCCAGATACGGGGATAG

Upstream 100 bases:

>100_bases
AAGGTAACGGTCAAGAGCCAAGGCTAGTCTAACTTTAACCTTGCCATTTTCCGGCCCAGAGTTTAATATTTGTGTATCAC
CGCGGCTTAGAGGAGAGACA

Downstream 100 bases:

>100_bases
CTTATTACGCTACTTCCGTAACTGAATATAATCTAAGGGGGCTTTAAAGCCCCCCTTTTAAATATCTGCTACACGGCTGA
CTAAAGCTACGGCCGGGTAA

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MEKALLEIEEKARLAKAASRPLSYASSAQKDAALKNIAACLLNNAPAILEANLKDQTEAKAAGMTAAMLDRLIITQSRLE
GIAKDTLAIAALPDPVGEIFDMNTMPNGLVIGKKRVPLGVIAAIYESRPNVTVDIAALCLKAGNAVILRGGKETIHSNTI
LARLIRQAVEEAGLPQEAVQFIENTEHSLVNHLLKLSDQIDLVIPRGGAGLISYVKQNSFIPVVAGGIGVVHIYVDADAN
IADAVNIAYNSKVQRPTVCNAMDTLLVHKDIAAAFLPVVAAEWSKAGVEIRADKTAMEILENASSCKLIPAAADDWGKEF
LALVAAVKVVDSLDEALSHIARYGSGHTESIVTQNYTSSQRFLNEVDAAAVMVNASTRFTDGSQFGLGAELGISTQKMHA
RGPMGLKEITSYKWIVYGNGQIRG

Sequences:

>Translated_424_residues
MEKALLEIEEKARLAKAASRPLSYASSAQKDAALKNIAACLLNNAPAILEANLKDQTEAKAAGMTAAMLDRLIITQSRLE
GIAKDTLAIAALPDPVGEIFDMNTMPNGLVIGKKRVPLGVIAAIYESRPNVTVDIAALCLKAGNAVILRGGKETIHSNTI
LARLIRQAVEEAGLPQEAVQFIENTEHSLVNHLLKLSDQIDLVIPRGGAGLISYVKQNSFIPVVAGGIGVVHIYVDADAN
IADAVNIAYNSKVQRPTVCNAMDTLLVHKDIAAAFLPVVAAEWSKAGVEIRADKTAMEILENASSCKLIPAAADDWGKEF
LALVAAVKVVDSLDEALSHIARYGSGHTESIVTQNYTSSQRFLNEVDAAAVMVNASTRFTDGSQFGLGAELGISTQKMHA
RGPMGLKEITSYKWIVYGNGQIRG
>Mature_424_residues
MEKALLEIEEKARLAKAASRPLSYASSAQKDAALKNIAACLLNNAPAILEANLKDQTEAKAAGMTAAMLDRLIITQSRLE
GIAKDTLAIAALPDPVGEIFDMNTMPNGLVIGKKRVPLGVIAAIYESRPNVTVDIAALCLKAGNAVILRGGKETIHSNTI
LARLIRQAVEEAGLPQEAVQFIENTEHSLVNHLLKLSDQIDLVIPRGGAGLISYVKQNSFIPVVAGGIGVVHIYVDADAN
IADAVNIAYNSKVQRPTVCNAMDTLLVHKDIAAAFLPVVAAEWSKAGVEIRADKTAMEILENASSCKLIPAAADDWGKEF
LALVAAVKVVDSLDEALSHIARYGSGHTESIVTQNYTSSQRFLNEVDAAAVMVNASTRFTDGSQFGLGAELGISTQKMHA
RGPMGLKEITSYKWIVYGNGQIRG

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=418, Percent_Identity=35.4066985645933, Blast_Score=258, Evalue=9e-69,
Organism=Homo sapiens, GI62912457, Length=418, Percent_Identity=35.4066985645933, Blast_Score=258, Evalue=1e-68,
Organism=Escherichia coli, GI1786438, Length=416, Percent_Identity=49.0384615384615, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17569967, Length=419, Percent_Identity=37.708830548926, Blast_Score=288, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI17569969, Length=419, Percent_Identity=37.708830548926, Blast_Score=288, Evalue=5e-78,
Organism=Saccharomyces cerevisiae, GI6324899, Length=427, Percent_Identity=36.768149882904, Blast_Score=263, Evalue=3e-71,
Organism=Drosophila melanogaster, GI21357643, Length=415, Percent_Identity=37.5903614457831, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_DEHE1 (Q3Z6Z9)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_182000.1
- ProteinModelPortal:   Q3Z6Z9
- SMR:   Q3Z6Z9
- STRING:   Q3Z6Z9
- GeneID:   3229380
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET1288
- NMPDR:   fig|243164.3.peg.1281
- TIGR:   DET1288
- eggNOG:   COG0014
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- PhylomeDB:   Q3Z6Z9
- ProtClustDB:   PRK00197
- BioCyc:   DETH243164:DET_1288-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45143; Mature: 45143

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKALLEIEEKARLAKAASRPLSYASSAQKDAALKNIAACLLNNAPAILEANLKDQTEAK
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
AAGMTAAMLDRLIITQSRLEGIAKDTLAIAALPDPVGEIFDMNTMPNGLVIGKKRVPLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHEECCCCCCCEEEECCCCCHHH
IAAIYESRPNVTVDIAALCLKAGNAVILRGGKETIHSNTILARLIRQAVEEAGLPQEAVQ
HHHHHHCCCCEEEHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FIENTEHSLVNHLLKLSDQIDLVIPRGGAGLISYVKQNSFIPVVAGGIGVVHIYVDADAN
HHHCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCEEEECCEEEEEEEEECCCC
IADAVNIAYNSKVQRPTVCNAMDTLLVHKDIAAAFLPVVAAEWSKAGVEIRADKTAMEIL
HHHHEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHH
ENASSCKLIPAAADDWGKEFLALVAAVKVVDSLDEALSHIARYGSGHTESIVTQNYTSSQ
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCCHHH
RFLNEVDAAAVMVNASTRFTDGSQFGLGAELGISTQKMHARGPMGLKEITSYKWIVYGNG
HHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHCCEEEEEEECC
QIRG
EECC
>Mature Secondary Structure
MEKALLEIEEKARLAKAASRPLSYASSAQKDAALKNIAACLLNNAPAILEANLKDQTEAK
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
AAGMTAAMLDRLIITQSRLEGIAKDTLAIAALPDPVGEIFDMNTMPNGLVIGKKRVPLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHEECCCCCCCEEEECCCCCHHH
IAAIYESRPNVTVDIAALCLKAGNAVILRGGKETIHSNTILARLIRQAVEEAGLPQEAVQ
HHHHHHCCCCEEEHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FIENTEHSLVNHLLKLSDQIDLVIPRGGAGLISYVKQNSFIPVVAGGIGVVHIYVDADAN
HHHCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCEEEECCEEEEEEEEECCCC
IADAVNIAYNSKVQRPTVCNAMDTLLVHKDIAAAFLPVVAAEWSKAGVEIRADKTAMEIL
HHHHEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHH
ENASSCKLIPAAADDWGKEFLALVAAVKVVDSLDEALSHIARYGSGHTESIVTQNYTSSQ
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCCHHH
RFLNEVDAAAVMVNASTRFTDGSQFGLGAELGISTQKMHARGPMGLKEITSYKWIVYGNG
HHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHCCEEEEEEECC
QIRG
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA