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The map label for this gene is groEL

Identifier: 57233780

GI number: 57233780

Start: 1298365

End: 1299978

Strand: Direct

Name: groEL

Synonym: DET1428

Alternate gene names: 57233780

Gene position: 1298365-1299978 (Clockwise)

Preceding gene: 57233781

Following gene: 57233778

Centisome position: 88.34

GC content: 53.84

Gene sequence:

>1614_bases
ATGGCTAAGCAGATTATATTTGGCGAAAAAGTCAGGGTCTCCCTCAAGAAGGGCGTTGATACTCTGGCTAACACAGTAAG
AGTAACCCTTGGTCCCAAAGGTCATCCCGTTGCCCTGGAGCGGAAATGGGGCGCACCCACTGTTATAGATGACGGTGTAA
CCATTGCCCGTGATATTGAACTGCCCGATGCTTTTGAAAATATGGGTGCCCAGCTGGTCAAAGAGGCCGCAACCCGCACC
AGCGATGCCGCCGGTGACGGTACTACCACCTCTATCGTACTGGCTCAGGCCCTTATCAACGAAGCCTTCAAGAATATTGC
CGCCGGTGCCGAACCCATCAACCTGAAACGCGGCATTGAAAAGGCTGTGGCCGCTCTTAAGGCCCAGCTCCGCAAGAACT
CCACCCCCGTCAAGGGCAAACAGCAGATTGTTCAGGTAGCCACCATCACCGGTAAAGACCCCGAAATCGGCAACCTGATT
GCTGACGTTATGGATAAAGTCGGCAAAGACGGTGTTATCACTATTGAAGAATCCCGTGGTCTGCGCTATGAAACCAGCTA
CGTAGAAGGTATGCAGTTTGACCGCGGTTATATCAGCGCTTACTTCGTAACTGACCCCGGCCGGATGGAATCCATCATGG
AAGATGCCACCATCCTGATGACCGACCGCAAGATTGAAACCGTTGCCGAGCTGCTGCCCGCTCTGGAAAAAATCCTGCAG
ATTTCAAAGAATCTGGTCATTGTAGCCGAAAACGTTGAAGCCGAAGCTCTGGCTACCCTGGTAGTCAACAAGCTGCGCGG
CAACCTGAATATCCTGGCTGTCAAAGCCCCCGGCTATGGCGACCGCCAGAAAGCTATGCTGGAAGATATGGCCATACTGA
CCGGCGGCCATGTAATCAGCAAGGAAGCCGGCCGCAAGCTGGATTCCGTAACCGAAGCTGACCTCGGTCATGCTCGCAGA
GTTGTCTCCAATAAAGACAAGACCACCATTATTGACGGCGAAGGCTCTGCCGAAGCTATCAAGAACCGCATCAAGCAGAT
TAAAGCCCAGATTGAAGAAACCGAATCTGCCTTTGACCGCGAAAAACTGCAGGAACGCCAGGCTGCTCTGGTCGGCGGCG
TAGCTGTTATCGCAGTAGGCGCTGCTACCGAAACCGAAATGAAAGAGCGCAAAGCCCGCGTTGAAGATGCTTTGGCTGCC
ACCCGTGCCGCCATTGAAGAAGGTATCCTGCCCGGCGGCGGTACCGGCCTCCTGAATGCCCTGCCCTGCCTTGATGCCCT
GAAACTGGAAGGCGACGAAGCCACCGGTGTCAGCATTGTCCGCAAGGCTCTTATTGAACCCGTCCGCTGGATTGCTACCA
ACGCCGGCAAAGACGGCAACGTTATCGTTGACAAGGTCAAGAACTCACCCGTAGGCCATGGTTACAACGCTGAGAAAGAT
GTCTTCGGCGATATGGCTGAAATGGGTATCATTGACCCCACCATGGTTGTCCGCTCTGCCCTTGAGAACGCTTCCTCCAT
TGCCAATATGGTTCTCATCACCGACTCACTGGTAGCTGATATTCAGGATAAAAACCCCGCTCCCCCCATGCCTGAAGCTC
CCGGAATGTACTAA

Upstream 100 bases:

>100_bases
TGAACTTAAATCTGGCGGTATTGATTACATCATCATGCCCGAAAGCCAGATTATGGCTAAAATCGTAGGCTAAATTTTTT
AACGAATATTAGGAGGTATA

Downstream 100 bases:

>100_bases
GACCCAAACGGGTTAAACAAGAAGGGCGGGACTTTAAAGTCCCGCCCTTTTCTATTATTCACAATAGGCTTAGATACAAA
TATATTGTCTAGTCTTTCAG

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MAKQIIFGEKVRVSLKKGVDTLANTVRVTLGPKGHPVALERKWGAPTVIDDGVTIARDIELPDAFENMGAQLVKEAATRT
SDAAGDGTTTSIVLAQALINEAFKNIAAGAEPINLKRGIEKAVAALKAQLRKNSTPVKGKQQIVQVATITGKDPEIGNLI
ADVMDKVGKDGVITIEESRGLRYETSYVEGMQFDRGYISAYFVTDPGRMESIMEDATILMTDRKIETVAELLPALEKILQ
ISKNLVIVAENVEAEALATLVVNKLRGNLNILAVKAPGYGDRQKAMLEDMAILTGGHVISKEAGRKLDSVTEADLGHARR
VVSNKDKTTIIDGEGSAEAIKNRIKQIKAQIEETESAFDREKLQERQAALVGGVAVIAVGAATETEMKERKARVEDALAA
TRAAIEEGILPGGGTGLLNALPCLDALKLEGDEATGVSIVRKALIEPVRWIATNAGKDGNVIVDKVKNSPVGHGYNAEKD
VFGDMAEMGIIDPTMVVRSALENASSIANMVLITDSLVADIQDKNPAPPMPEAPGMY

Sequences:

>Translated_537_residues
MAKQIIFGEKVRVSLKKGVDTLANTVRVTLGPKGHPVALERKWGAPTVIDDGVTIARDIELPDAFENMGAQLVKEAATRT
SDAAGDGTTTSIVLAQALINEAFKNIAAGAEPINLKRGIEKAVAALKAQLRKNSTPVKGKQQIVQVATITGKDPEIGNLI
ADVMDKVGKDGVITIEESRGLRYETSYVEGMQFDRGYISAYFVTDPGRMESIMEDATILMTDRKIETVAELLPALEKILQ
ISKNLVIVAENVEAEALATLVVNKLRGNLNILAVKAPGYGDRQKAMLEDMAILTGGHVISKEAGRKLDSVTEADLGHARR
VVSNKDKTTIIDGEGSAEAIKNRIKQIKAQIEETESAFDREKLQERQAALVGGVAVIAVGAATETEMKERKARVEDALAA
TRAAIEEGILPGGGTGLLNALPCLDALKLEGDEATGVSIVRKALIEPVRWIATNAGKDGNVIVDKVKNSPVGHGYNAEKD
VFGDMAEMGIIDPTMVVRSALENASSIANMVLITDSLVADIQDKNPAPPMPEAPGMY
>Mature_536_residues
AKQIIFGEKVRVSLKKGVDTLANTVRVTLGPKGHPVALERKWGAPTVIDDGVTIARDIELPDAFENMGAQLVKEAATRTS
DAAGDGTTTSIVLAQALINEAFKNIAAGAEPINLKRGIEKAVAALKAQLRKNSTPVKGKQQIVQVATITGKDPEIGNLIA
DVMDKVGKDGVITIEESRGLRYETSYVEGMQFDRGYISAYFVTDPGRMESIMEDATILMTDRKIETVAELLPALEKILQI
SKNLVIVAENVEAEALATLVVNKLRGNLNILAVKAPGYGDRQKAMLEDMAILTGGHVISKEAGRKLDSVTEADLGHARRV
VSNKDKTTIIDGEGSAEAIKNRIKQIKAQIEETESAFDREKLQERQAALVGGVAVIAVGAATETEMKERKARVEDALAAT
RAAIEEGILPGGGTGLLNALPCLDALKLEGDEATGVSIVRKALIEPVRWIATNAGKDGNVIVDKVKNSPVGHGYNAEKDV
FGDMAEMGIIDPTMVVRSALENASSIANMVLITDSLVADIQDKNPAPPMPEAPGMY

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=530, Percent_Identity=44.9056603773585, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI31542947, Length=530, Percent_Identity=44.9056603773585, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI24307939, Length=430, Percent_Identity=26.5116279069767, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1790586, Length=528, Percent_Identity=53.030303030303, Blast_Score=540, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17555558, Length=524, Percent_Identity=43.8931297709924, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI193210679, Length=206, Percent_Identity=45.6310679611651, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17564182, Length=548, Percent_Identity=23.3576642335766, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=46.5648854961832, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=47.7272727272727, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=47.7272727272727, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI45550936, Length=523, Percent_Identity=44.7418738049713, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45550132, Length=523, Percent_Identity=44.7418738049713, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45550935, Length=523, Percent_Identity=44.7418738049713, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI17864606, Length=538, Percent_Identity=39.5910780669145, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24584129, Length=534, Percent_Identity=34.4569288389513, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI19921262, Length=534, Percent_Identity=34.4569288389513, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI18858175, Length=564, Percent_Identity=24.290780141844, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571140, Length=564, Percent_Identity=24.290780141844, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_DEHE1 (Q3Z6L3)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_182136.1
- HSSP:   P0A6F5
- ProteinModelPortal:   Q3Z6L3
- SMR:   Q3Z6L3
- STRING:   Q3Z6L3
- GeneID:   3229243
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET1428
- NMPDR:   fig|243164.3.peg.1396
- TIGR:   DET1428
- eggNOG:   COG0459
- HOGENOM:   HBG625289
- OMA:   MEDATIL
- ProtClustDB:   PRK00013
- BioCyc:   DETH243164:DET_1428-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57283; Mature: 57151

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQIIFGEKVRVSLKKGVDTLANTVRVTLGPKGHPVALERKWGAPTVIDDGVTIARDIE
CCCCEECCCHHHHHHHHHHHHHHHCEEEEECCCCCCEEEEECCCCCCEEECCCEEEEECC
LPDAFENMGAQLVKEAATRTSDAAGDGTTTSIVLAQALINEAFKNIAAGAEPINLKRGIE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
KAVAALKAQLRKNSTPVKGKQQIVQVATITGKDPEIGNLIADVMDKVGKDGVITIEESRG
HHHHHHHHHHHCCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
LRYETSYVEGMQFDRGYISAYFVTDPGRMESIMEDATILMTDRKIETVAELLPALEKILQ
CEEEHHHHCCCCCCCCEEEEEEEECCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHH
ISKNLVIVAENVEAEALATLVVNKLRGNLNILAVKAPGYGDRQKAMLEDMAILTGGHVIS
HCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEC
KEAGRKLDSVTEADLGHARRVVSNKDKTTIIDGEGSAEAIKNRIKQIKAQIEETESAFDR
HHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLQERQAALVGGVAVIAVGAATETEMKERKARVEDALAATRAAIEEGILPGGGTGLLNA
HHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LPCLDALKLEGDEATGVSIVRKALIEPVRWIATNAGKDGNVIVDKVKNSPVGHGYNAEKD
HHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHH
VFGDMAEMGIIDPTMVVRSALENASSIANMVLITDSLVADIQDKNPAPPMPEAPGMY
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKQIIFGEKVRVSLKKGVDTLANTVRVTLGPKGHPVALERKWGAPTVIDDGVTIARDIE
CCCEECCCHHHHHHHHHHHHHHHCEEEEECCCCCCEEEEECCCCCCEEECCCEEEEECC
LPDAFENMGAQLVKEAATRTSDAAGDGTTTSIVLAQALINEAFKNIAAGAEPINLKRGIE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
KAVAALKAQLRKNSTPVKGKQQIVQVATITGKDPEIGNLIADVMDKVGKDGVITIEESRG
HHHHHHHHHHHCCCCCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
LRYETSYVEGMQFDRGYISAYFVTDPGRMESIMEDATILMTDRKIETVAELLPALEKILQ
CEEEHHHHCCCCCCCCEEEEEEEECCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHH
ISKNLVIVAENVEAEALATLVVNKLRGNLNILAVKAPGYGDRQKAMLEDMAILTGGHVIS
HCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEC
KEAGRKLDSVTEADLGHARRVVSNKDKTTIIDGEGSAEAIKNRIKQIKAQIEETESAFDR
HHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLQERQAALVGGVAVIAVGAATETEMKERKARVEDALAATRAAIEEGILPGGGTGLLNA
HHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LPCLDALKLEGDEATGVSIVRKALIEPVRWIATNAGKDGNVIVDKVKNSPVGHGYNAEKD
HHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHH
VFGDMAEMGIIDPTMVVRSALENASSIANMVLITDSLVADIQDKNPAPPMPEAPGMY
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA