Click here to switch to the map view.

The map label for this gene is ispF

Identifier: 57233568

GI number: 57233568

Start: 55826

End: 56299

Strand: Direct

Name: ispF

Synonym: DET0060

Alternate gene names: 57233568

Gene position: 55826-56299 (Clockwise)

Preceding gene: 57233576

Following gene: 57233567

Centisome position: 3.8

GC content: 55.27

Gene sequence:

>474_bases
ATGCGTATTGGAAACGGTTATGATGTACATCGTCTGGCACCGGGGCAGAAGCTGGTGCTGGGCGGGGTGGAAATACCCTT
TGAGTGCGGGCTTATCGGCTGGAGTGATGCGGACGTGCTTACCCACGCCATTATGGATTCGCTTCTGGGGGCGGCGGCAC
TGGGGGATATAGGGCTTTACTTCCCGCCGGGTGACCCTAAGTTCAAGGGCATATCTTCACTTAAACTCCTTGAGCAGGTA
ACAGCCTTGCTGGCTGAAAAAGGTTTCGGGATAATAAACGTAGATTCAGTTATAGTAGCGGAGGAACCAAAGCTACGCGG
CCATGTAGATACCATGCGTAAACACCTTGCCAAGGCCATGGGCATAGACCCGGGGCGGGTAGGGATAAAAGCCAGCACCT
CGGAGCAACTCGGCTTTGTAGGCCGCGAGGAGGGCATGGCAGCCTGGGCAGTGGCCCTCGTAGACGAAAAATAG

Upstream 100 bases:

>100_bases
CGGTCAAGATATTCTCCGGCGCATATGCTAATATAAAGATAACCACACCCGAAGACCTGCTTATGGCAGAAATACTGCTG
AAGAAAGGACGGTGAGCATA

Downstream 100 bases:

>100_bases
ACTATGAAAATATATAACACTTTATCCGGCAAGCTGGAAGAATTTGTCCCTCTGGAAGATGGCAAGGTAAAAATGTATGT
CTGCGGCATAACCCCGCAGT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRIGNGYDVHRLAPGQKLVLGGVEIPFECGLIGWSDADVLTHAIMDSLLGAAALGDIGLYFPPGDPKFKGISSLKLLEQV
TALLAEKGFGIINVDSVIVAEEPKLRGHVDTMRKHLAKAMGIDPGRVGIKASTSEQLGFVGREEGMAAWAVALVDEK

Sequences:

>Translated_157_residues
MRIGNGYDVHRLAPGQKLVLGGVEIPFECGLIGWSDADVLTHAIMDSLLGAAALGDIGLYFPPGDPKFKGISSLKLLEQV
TALLAEKGFGIINVDSVIVAEEPKLRGHVDTMRKHLAKAMGIDPGRVGIKASTSEQLGFVGREEGMAAWAVALVDEK
>Mature_157_residues
MRIGNGYDVHRLAPGQKLVLGGVEIPFECGLIGWSDADVLTHAIMDSLLGAAALGDIGLYFPPGDPKFKGISSLKLLEQV
TALLAEKGFGIINVDSVIVAEEPKLRGHVDTMRKHLAKAMGIDPGRVGIKASTSEQLGFVGREEGMAAWAVALVDEK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=48.7012987012987, Blast_Score=138, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_DEHE1 (Q3ZAD6)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_180813.1
- ProteinModelPortal:   Q3ZAD6
- SMR:   Q3ZAD6
- STRING:   Q3ZAD6
- GeneID:   3229030
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0060
- NMPDR:   fig|243164.3.peg.70
- TIGR:   DET0060
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   Q3ZAD6
- ProtClustDB:   PRK00084
- BioCyc:   DETH243164:DET_0060-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16634; Mature: 16634

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGNGYDVHRLAPGQKLVLGGVEIPFECGLIGWSDADVLTHAIMDSLLGAAALGDIGLY
CCCCCCCCEEEECCCCEEEECCCCCCEECCEECCCHHHHHHHHHHHHHHHHHHHCCCEEE
FPPGDPKFKGISSLKLLEQVTALLAEKGFGIINVDSVIVAEEPKLRGHVDTMRKHLAKAM
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCCCHHHHHHHHHHHH
GIDPGRVGIKASTSEQLGFVGREEGMAAWAVALVDEK
CCCCCCCEEEECCCHHHCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MRIGNGYDVHRLAPGQKLVLGGVEIPFECGLIGWSDADVLTHAIMDSLLGAAALGDIGLY
CCCCCCCCEEEECCCCEEEECCCCCCEECCEECCCHHHHHHHHHHHHHHHHHHHCCCEEE
FPPGDPKFKGISSLKLLEQVTALLAEKGFGIINVDSVIVAEEPKLRGHVDTMRKHLAKAM
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCCCHHHHHHHHHHHH
GIDPGRVGIKASTSEQLGFVGREEGMAAWAVALVDEK
CCCCCCCEEEECCCHHHCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA