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The map label for this gene is cysS

Identifier: 57233567

GI number: 57233567

Start: 56303

End: 57679

Strand: Direct

Name: cysS

Synonym: DET0061

Alternate gene names: 57233567

Gene position: 56303-57679 (Clockwise)

Preceding gene: 57233568

Following gene: 57233559

Centisome position: 3.83

GC content: 50.62

Gene sequence:

>1377_bases
ATGAAAATATATAACACTTTATCCGGCAAGCTGGAAGAATTTGTCCCTCTGGAAGATGGCAAGGTAAAAATGTATGTCTG
CGGCATAACCCCGCAGTCAGAGCCGCATATCGGCCATGCCATGAGCTATATAAATTTTGACGTAATCCGCCGTTACCTCA
GCTACAAAGGCTACCGGGTAAAATATATCCAGAATTTCACGGATATAGATGACAAAATAATTGCCAAAGCCAACGCTCAG
GGCATAGAGCCGTCTACTCTGGCCGAAAGAAATATCGGGGTATTTCTGGATGCCATGGCCGCACTCAACATAACCCCGGC
GGACTACTACCCGCGGGCCACCCAGGAAGTACCCAAGATAATAGAAATGGTCTCCGGCCTTATAGAAAAGGGCTATGCAT
ACGAGGCAGGCGGCAGTGTCTACCTGCGGGTGCAGAAGGTGGAAGGCTACGGCAAACTGTCCCACCGCACTCTGGAGCAG
ATGATGGCCGGTGCCCGCATAGAGCCGGGCGAAGAAAAAGAATACCCAATGGACTTTGCCCTTTGGAAGGCTACCAAGCC
GGGAGAGCCTTCCTGGGAAAGCCCCTGGGGATTGGGACGCCCCGGCTGGCATATTGAGTGTTCGGCCATGTCTCTGCGTT
ATCTGGGAGAGCAGATAGATATACACGGCGGCGGGCAGGACCTTATTTTCCCCCACCATGAAAACGAAATCGCTCAGTCC
GAATGTTTCAGCGGGGTCAAACCCTTTGTCAAATACTGGCTGCATAACGGTCTTCTCAAGCTTGGCGAAGAGAAAATGAG
CAAGTCGCTGGGTAATCTGGTCACCATAAAAGAAGCCCTCAGCCGCTATTCGGCTGATGCCCTGCGTATCTTTGTACTCA
GCTCCAGCTACCGTAATCCGCTTACTTACTCTGAAGAAGCTCTGGAAGCAGCCGAAAAAGGGGCGGAACGCCTGCGCCAG
ACAGCCGCCAGACAGGATAATCCCCGGTTACAAGAAATAAATGTAGATGTTAAAACATACCGTGAGCGGTTTACCCAGTA
TATGGACAATGACTTTAACACCTCAGCCGCTCTGGCTACTATATTTGACCTTGGACGTGAGCTAAACCGCATTGAAGCAG
AGGGTGGCAAAAGCACACAAGGGCAAGAACTGTTTAAAGAGCTGGCTGGTATACTTGGACTTAGCCTGATAATCGCGGAG
CCTACGACTAGTGCAGATGCCGCGCCTTTTATAGATATGCTGATAGAACTCAGAAAAGACCTGCGTCTGGCAAAGCAGTA
CCAGCTGGCAGACAAAATCCGTACCAGCCTGGATGCAGCCGGGATACTTCTGGAAGACTCTGCCGGCGGCACTATATGGA
AAATAAAAACCAAGTAA

Upstream 100 bases:

>100_bases
GGTAGGGATAAAAGCCAGCACCTCGGAGCAACTCGGCTTTGTAGGCCGCGAGGAGGGCATGGCAGCCTGGGCAGTGGCCC
TCGTAGACGAAAAATAGACT

Downstream 100 bases:

>100_bases
TCGCCGAAGATTTCAGTATAACTATTGACTCTGCGGTTTCGCTGTAAAGCTAAAAAAAGTTGCACTTGGTGTCCATTTGT
GATAGATTATCTATCTGTTG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKIYNTLSGKLEEFVPLEDGKVKMYVCGITPQSEPHIGHAMSYINFDVIRRYLSYKGYRVKYIQNFTDIDDKIIAKANAQ
GIEPSTLAERNIGVFLDAMAALNITPADYYPRATQEVPKIIEMVSGLIEKGYAYEAGGSVYLRVQKVEGYGKLSHRTLEQ
MMAGARIEPGEEKEYPMDFALWKATKPGEPSWESPWGLGRPGWHIECSAMSLRYLGEQIDIHGGGQDLIFPHHENEIAQS
ECFSGVKPFVKYWLHNGLLKLGEEKMSKSLGNLVTIKEALSRYSADALRIFVLSSSYRNPLTYSEEALEAAEKGAERLRQ
TAARQDNPRLQEINVDVKTYRERFTQYMDNDFNTSAALATIFDLGRELNRIEAEGGKSTQGQELFKELAGILGLSLIIAE
PTTSADAAPFIDMLIELRKDLRLAKQYQLADKIRTSLDAAGILLEDSAGGTIWKIKTK

Sequences:

>Translated_458_residues
MKIYNTLSGKLEEFVPLEDGKVKMYVCGITPQSEPHIGHAMSYINFDVIRRYLSYKGYRVKYIQNFTDIDDKIIAKANAQ
GIEPSTLAERNIGVFLDAMAALNITPADYYPRATQEVPKIIEMVSGLIEKGYAYEAGGSVYLRVQKVEGYGKLSHRTLEQ
MMAGARIEPGEEKEYPMDFALWKATKPGEPSWESPWGLGRPGWHIECSAMSLRYLGEQIDIHGGGQDLIFPHHENEIAQS
ECFSGVKPFVKYWLHNGLLKLGEEKMSKSLGNLVTIKEALSRYSADALRIFVLSSSYRNPLTYSEEALEAAEKGAERLRQ
TAARQDNPRLQEINVDVKTYRERFTQYMDNDFNTSAALATIFDLGRELNRIEAEGGKSTQGQELFKELAGILGLSLIIAE
PTTSADAAPFIDMLIELRKDLRLAKQYQLADKIRTSLDAAGILLEDSAGGTIWKIKTK
>Mature_458_residues
MKIYNTLSGKLEEFVPLEDGKVKMYVCGITPQSEPHIGHAMSYINFDVIRRYLSYKGYRVKYIQNFTDIDDKIIAKANAQ
GIEPSTLAERNIGVFLDAMAALNITPADYYPRATQEVPKIIEMVSGLIEKGYAYEAGGSVYLRVQKVEGYGKLSHRTLEQ
MMAGARIEPGEEKEYPMDFALWKATKPGEPSWESPWGLGRPGWHIECSAMSLRYLGEQIDIHGGGQDLIFPHHENEIAQS
ECFSGVKPFVKYWLHNGLLKLGEEKMSKSLGNLVTIKEALSRYSADALRIFVLSSSYRNPLTYSEEALEAAEKGAERLRQ
TAARQDNPRLQEINVDVKTYRERFTQYMDNDFNTSAALATIFDLGRELNRIEAEGGKSTQGQELFKELAGILGLSLIIAE
PTTSADAAPFIDMLIELRKDLRLAKQYQLADKIRTSLDAAGILLEDSAGGTIWKIKTK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=314, Percent_Identity=45.2229299363057, Blast_Score=268, Evalue=1e-71,
Organism=Homo sapiens, GI10835051, Length=232, Percent_Identity=46.551724137931, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI303304987, Length=232, Percent_Identity=46.551724137931, Blast_Score=211, Evalue=9e-55,
Organism=Homo sapiens, GI21269877, Length=232, Percent_Identity=46.551724137931, Blast_Score=211, Evalue=9e-55,
Organism=Homo sapiens, GI62240992, Length=232, Percent_Identity=46.551724137931, Blast_Score=211, Evalue=1e-54,
Organism=Escherichia coli, GI1786737, Length=466, Percent_Identity=45.4935622317597, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17509795, Length=239, Percent_Identity=43.0962343096234, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17509797, Length=239, Percent_Identity=43.0962343096234, Blast_Score=188, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6324082, Length=413, Percent_Identity=34.1404358353511, Blast_Score=195, Evalue=1e-50,
Organism=Drosophila melanogaster, GI19922218, Length=502, Percent_Identity=32.4701195219124, Blast_Score=231, Evalue=5e-61,
Organism=Drosophila melanogaster, GI19922364, Length=233, Percent_Identity=45.0643776824034, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_DEHE1 (Q3ZAD5)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_180814.1
- ProteinModelPortal:   Q3ZAD5
- SMR:   Q3ZAD5
- STRING:   Q3ZAD5
- GeneID:   3229029
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0061
- NMPDR:   fig|243164.3.peg.71
- TIGR:   DET0061
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   Q3ZAD5
- ProtClustDB:   PRK00260
- BioCyc:   DETH243164:DET_0061-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 51374; Mature: 51374

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 268-268

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYNTLSGKLEEFVPLEDGKVKMYVCGITPQSEPHIGHAMSYINFDVIRRYLSYKGYRV
CCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
KYIQNFTDIDDKIIAKANAQGIEPSTLAERNIGVFLDAMAALNITPADYYPRATQEVPKI
EEECCCCCCCHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHH
IEMVSGLIEKGYAYEAGGSVYLRVQKVEGYGKLSHRTLEQMMAGARIEPGEEKEYPMDFA
HHHHHHHHHCCCEEECCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
LWKATKPGEPSWESPWGLGRPGWHIECSAMSLRYLGEQIDIHGGGQDLIFPHHENEIAQS
EEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCEEEECCCCCCEECCCCCCHHHHH
ECFSGVKPFVKYWLHNGLLKLGEEKMSKSLGNLVTIKEALSRYSADALRIFVLSSSYRNP
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCC
LTYSEEALEAAEKGAERLRQTAARQDNPRLQEINVDVKTYRERFTQYMDNDFNTSAALAT
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHH
IFDLGRELNRIEAEGGKSTQGQELFKELAGILGLSLIIAEPTTSADAAPFIDMLIELRKD
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHH
LRLAKQYQLADKIRTSLDAAGILLEDSAGGTIWKIKTK
HHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEECC
>Mature Secondary Structure
MKIYNTLSGKLEEFVPLEDGKVKMYVCGITPQSEPHIGHAMSYINFDVIRRYLSYKGYRV
CCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
KYIQNFTDIDDKIIAKANAQGIEPSTLAERNIGVFLDAMAALNITPADYYPRATQEVPKI
EEECCCCCCCHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHH
IEMVSGLIEKGYAYEAGGSVYLRVQKVEGYGKLSHRTLEQMMAGARIEPGEEKEYPMDFA
HHHHHHHHHCCCEEECCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
LWKATKPGEPSWESPWGLGRPGWHIECSAMSLRYLGEQIDIHGGGQDLIFPHHENEIAQS
EEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCEEEECCCCCCEECCCCCCHHHHH
ECFSGVKPFVKYWLHNGLLKLGEEKMSKSLGNLVTIKEALSRYSADALRIFVLSSSYRNP
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCC
LTYSEEALEAAEKGAERLRQTAARQDNPRLQEINVDVKTYRERFTQYMDNDFNTSAALAT
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHH
IFDLGRELNRIEAEGGKSTQGQELFKELAGILGLSLIIAEPTTSADAAPFIDMLIELRKD
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHH
LRLAKQYQLADKIRTSLDAAGILLEDSAGGTIWKIKTK
HHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA