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The map label for this gene is trpB2 [H]

Identifier: 38234893

GI number: 38234893

Start: 2465139

End: 2466365

Strand: Direct

Name: trpB2 [H]

Synonym: DIP2360

Alternate gene names: 38234893

Gene position: 2465139-2466365 (Clockwise)

Preceding gene: 38234891

Following gene: 38234894

Centisome position: 99.06

GC content: 57.95

Gene sequence:

>1227_bases
ATGAAACACACACTGCTACCGGCATACTTCGGAGAATTCGGCGGACAATACGTACCCGAGTCCCTTATCCCCGCCCTCGA
CCAATTAGAAGCGGCCTTCGTGGAAGCGCAAAACGATCCCGAGTTTAGAGCCGAACTCGCCGGCTACCTCAAGGACTACC
TCGGTCGTCCCACGCCGCTCACGCTCTGCTCCAAGCTACCCAAAGCCGGAAAAGGGAAGGGGCACGCGCGGATCTTCCTC
AAACGAGAAGACCTCGTTCACGGCGGCGCACACAAAACCAACCAAGTAATCGGCCAAGCCCTCTTAGCCAAACGCATGGG
TAAAACCCGCATTATTGCTGAAACCGGTGCCGGACAACACGGAACCGCCACCGCACTCGCATGCGCACTGCTCGACCTCG
ACTGCGTGATCTACATGGGCGCTAAAGACGTGGAACGCCAACAACCCAACGTGTACCGCATGGAGCTCATGGGCGCCAAG
GTCGTACCAGTCGACTCCGGCTCCGGAACCCTCAAAGATGCAGTCAACGAAGCACTGCGCGATTGGACCGCAACCTTCCA
CGAGTCACACTATCTGCTCGGCACCGCAGCCGGGCCACACCCATTTCCCACGATTGTGCGCGAATTCCACCGCGTAATCT
CAGAAGAAGCCAAAGCCCAAATACTTGAGCAAACCGGCGCACTGCCAGATGTGGTGGTCGCCTGCGTCGGCGGCGGATCC
AACGCTATCGGCATGTTCGCCGACTTTATCGACGACCCCTCAGTAGAACTCGTCGGTGCAGAACCAGGCGGCATGGGACT
AAGCTCCGGAAAACACGGCGCTACCATCAACAACGGCCAAATCGGTATTTTGCACGGCGCCAGAAGCTACCTCATGCGCA
ACTCCGATGGCCAAGTAGAGGAATCCTATTCCATTTCAGCCGGCCTCGACTACCCAGGTGTCGGCCCACAACACGCCCAT
CTAGCAAAGTCTGGGCGCGCCCGCTATGTCGCCATCACTGATGCGGAAGCACTCGAAGCATTCCAATTGCTCTCCCGCGA
AGAGGGCATCATCCCGGCCTTGGAATCCAGCCACGCTCTTGCTTATGCACTCAAACGTGCCGCCCTCGCTGAGGAAAACA
ACGAAGATATCACCATCGTGGTGTCCTTGTCTGGCCGTGGCGACAAAGACATCGACCACATCCGCCGCACTCTTTCCGCC
CACCCAGAGCTGGTTGTAAAGGACTAA

Upstream 100 bases:

>100_bases
GAGCATTCACCACACAACTTTTGGAAACACAAGCCCGCGTATCGCGGGCTTTTTCGTATATTCCCTGCGATACGCCCAAC
CAACCACAGGGAATATCCTC

Downstream 100 bases:

>100_bases
AAACATGACTCGCTACACCAAATTATTCTCCGCACTCAGCGCCAAAAACGAAGGTGCATTCGTCCCCTTCATCATGCTGG
GTGCTCCCACCCCAGAGGCG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKHTLLPAYFGEFGGQYVPESLIPALDQLEAAFVEAQNDPEFRAELAGYLKDYLGRPTPLTLCSKLPKAGKGKGHARIFL
KREDLVHGGAHKTNQVIGQALLAKRMGKTRIIAETGAGQHGTATALACALLDLDCVIYMGAKDVERQQPNVYRMELMGAK
VVPVDSGSGTLKDAVNEALRDWTATFHESHYLLGTAAGPHPFPTIVREFHRVISEEAKAQILEQTGALPDVVVACVGGGS
NAIGMFADFIDDPSVELVGAEPGGMGLSSGKHGATINNGQIGILHGARSYLMRNSDGQVEESYSISAGLDYPGVGPQHAH
LAKSGRARYVAITDAEALEAFQLLSREEGIIPALESSHALAYALKRAALAEENNEDITIVVSLSGRGDKDIDHIRRTLSA
HPELVVKD

Sequences:

>Translated_408_residues
MKHTLLPAYFGEFGGQYVPESLIPALDQLEAAFVEAQNDPEFRAELAGYLKDYLGRPTPLTLCSKLPKAGKGKGHARIFL
KREDLVHGGAHKTNQVIGQALLAKRMGKTRIIAETGAGQHGTATALACALLDLDCVIYMGAKDVERQQPNVYRMELMGAK
VVPVDSGSGTLKDAVNEALRDWTATFHESHYLLGTAAGPHPFPTIVREFHRVISEEAKAQILEQTGALPDVVVACVGGGS
NAIGMFADFIDDPSVELVGAEPGGMGLSSGKHGATINNGQIGILHGARSYLMRNSDGQVEESYSISAGLDYPGVGPQHAH
LAKSGRARYVAITDAEALEAFQLLSREEGIIPALESSHALAYALKRAALAEENNEDITIVVSLSGRGDKDIDHIRRTLSA
HPELVVKD
>Mature_408_residues
MKHTLLPAYFGEFGGQYVPESLIPALDQLEAAFVEAQNDPEFRAELAGYLKDYLGRPTPLTLCSKLPKAGKGKGHARIFL
KREDLVHGGAHKTNQVIGQALLAKRMGKTRIIAETGAGQHGTATALACALLDLDCVIYMGAKDVERQQPNVYRMELMGAK
VVPVDSGSGTLKDAVNEALRDWTATFHESHYLLGTAAGPHPFPTIVREFHRVISEEAKAQILEQTGALPDVVVACVGGGS
NAIGMFADFIDDPSVELVGAEPGGMGLSSGKHGATINNGQIGILHGARSYLMRNSDGQVEESYSISAGLDYPGVGPQHAH
LAKSGRARYVAITDAEALEAFQLLSREEGIIPALESSHALAYALKRAALAEENNEDITIVVSLSGRGDKDIDHIRRTLSA
HPELVVKD

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=401, Percent_Identity=65.8354114713217, Blast_Score=534, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6321412, Length=393, Percent_Identity=51.1450381679389, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 43616; Mature: 43616

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHTLLPAYFGEFGGQYVPESLIPALDQLEAAFVEAQNDPEFRAELAGYLKDYLGRPTPL
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCH
TLCSKLPKAGKGKGHARIFLKREDLVHGGAHKTNQVIGQALLAKRMGKTRIIAETGAGQH
HHHHHCCCCCCCCCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GTATALACALLDLDCVIYMGAKDVERQQPNVYRMELMGAKVVPVDSGSGTLKDAVNEALR
CHHHHHHHHHHHHCEEEEECCHHHHHCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHH
DWTATFHESHYLLGTAAGPHPFPTIVREFHRVISEEAKAQILEQTGALPDVVVACVGGGS
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCC
NAIGMFADFIDDPSVELVGAEPGGMGLSSGKHGATINNGQIGILHGARSYLMRNSDGQVE
CHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEECCCCEEEEECHHHHHEECCCCCCC
ESYSISAGLDYPGVGPQHAHLAKSGRARYVAITDAEALEAFQLLSREEGIIPALESSHAL
CCCEEECCCCCCCCCCCHHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHH
AYALKRAALAEENNEDITIVVSLSGRGDKDIDHIRRTLSAHPELVVKD
HHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MKHTLLPAYFGEFGGQYVPESLIPALDQLEAAFVEAQNDPEFRAELAGYLKDYLGRPTPL
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCH
TLCSKLPKAGKGKGHARIFLKREDLVHGGAHKTNQVIGQALLAKRMGKTRIIAETGAGQH
HHHHHCCCCCCCCCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GTATALACALLDLDCVIYMGAKDVERQQPNVYRMELMGAKVVPVDSGSGTLKDAVNEALR
CHHHHHHHHHHHHCEEEEECCHHHHHCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHH
DWTATFHESHYLLGTAAGPHPFPTIVREFHRVISEEAKAQILEQTGALPDVVVACVGGGS
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCC
NAIGMFADFIDDPSVELVGAEPGGMGLSSGKHGATINNGQIGILHGARSYLMRNSDGQVE
CHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEECCCCEEEEECHHHHHEECCCCCCC
ESYSISAGLDYPGVGPQHAHLAKSGRARYVAITDAEALEAFQLLSREEGIIPALESSHAL
CCCEEECCCCCCCCCCCHHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHH
AYALKRAALAEENNEDITIVVSLSGRGDKDIDHIRRTLSAHPELVVKD
HHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]