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The map label for this gene is trpA

Identifier: 38234894

GI number: 38234894

Start: 2466370

End: 2467206

Strand: Direct

Name: trpA

Synonym: DIP2361

Alternate gene names: 38234894

Gene position: 2466370-2467206 (Clockwise)

Preceding gene: 38234893

Following gene: 38234895

Centisome position: 99.11

GC content: 59.38

Gene sequence:

>837_bases
ATGACTCGCTACACCAAATTATTCTCCGCACTCAGCGCCAAAAACGAAGGTGCATTCGTCCCCTTCATCATGCTGGGTGC
TCCCACCCCAGAGGCGTCGTTAGCAATCATCCGTACCGTCGTTGCCGCGGGTGCCGACGCCCTCGAACTCGGCGTCCCCT
TCTCCGACCCAGTAGCAGACGGCCCCACAATCCAACGCTCCCACCTCCGTGCCCTCGACAACGGAGCCACCGTCGATAGC
TCGCTGGAACTGATCCGACAGATCCGCAAAGAATTCCCCGAACTTCCCATCGGCATGCTCATCTACGGCAACGTTGCCTT
CACCCGCGGGATCACCCAGTTCTATCAAGAATTCGCCGACGCCGGCGTAGACTCCATCCTGCTTCCCGACGTCCCTGTCC
GCGAAGGCGAGCCTTTCATCGCAGCCGCCAAGCAAGCTGGAATCGACCCCATCTTCATCGCCCCCGCACAAGCCTCGGAA
GCAACCTTGGAAGGGGTAGCGCAACACTCCAGCGGATACATCTACGCTATCTCCCGCGATGGTGTGACCGGAACCGAACG
CCAATCCTCTACTAGGGGACTAGACAAAGTGGTGGCAAATGTGAAACGTTTCGGCGGCGCACCGATCCTGCTCGGTTTTG
GTATCTCCACTCCCGAGCATGTGCGCGACGCCATCGCGGCAGGAGCCAGCGGCGCCATTACAGGCTCCGCGCTGACGTCG
ATCATCGAGCGGCACACCACAGGCACACACCCAGAGCCAGCCCAAGTGACAGACCTTGAGGCTCTCACCGAAGAAATTTT
CGCGTTCGTTAAAGACATGAAAGCCGCAACCCGTTAA

Upstream 100 bases:

>100_bases
ACCATCGTGGTGTCCTTGTCTGGCCGTGGCGACAAAGACATCGACCACATCCGCCGCACTCTTTCCGCCCACCCAGAGCT
GGTTGTAAAGGACTAAAAAC

Downstream 100 bases:

>100_bases
ATCACTGTGACAAAACATGAATGTGTGATACTGGAAGGCATGACACATCCATGTTCTCGCCGCATGTTCCTCCTCGGGTC
CGCAACCACACTCGCTGGAG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTRYTKLFSALSAKNEGAFVPFIMLGAPTPEASLAIIRTVVAAGADALELGVPFSDPVADGPTIQRSHLRALDNGATVDS
SLELIRQIRKEFPELPIGMLIYGNVAFTRGITQFYQEFADAGVDSILLPDVPVREGEPFIAAAKQAGIDPIFIAPAQASE
ATLEGVAQHSSGYIYAISRDGVTGTERQSSTRGLDKVVANVKRFGGAPILLGFGISTPEHVRDAIAAGASGAITGSALTS
IIERHTTGTHPEPAQVTDLEALTEEIFAFVKDMKAATR

Sequences:

>Translated_278_residues
MTRYTKLFSALSAKNEGAFVPFIMLGAPTPEASLAIIRTVVAAGADALELGVPFSDPVADGPTIQRSHLRALDNGATVDS
SLELIRQIRKEFPELPIGMLIYGNVAFTRGITQFYQEFADAGVDSILLPDVPVREGEPFIAAAKQAGIDPIFIAPAQASE
ATLEGVAQHSSGYIYAISRDGVTGTERQSSTRGLDKVVANVKRFGGAPILLGFGISTPEHVRDAIAAGASGAITGSALTS
IIERHTTGTHPEPAQVTDLEALTEEIFAFVKDMKAATR
>Mature_277_residues
TRYTKLFSALSAKNEGAFVPFIMLGAPTPEASLAIIRTVVAAGADALELGVPFSDPVADGPTIQRSHLRALDNGATVDSS
LELIRQIRKEFPELPIGMLIYGNVAFTRGITQFYQEFADAGVDSILLPDVPVREGEPFIAAAKQAGIDPIFIAPAQASEA
TLEGVAQHSSGYIYAISRDGVTGTERQSSTRGLDKVVANVKRFGGAPILLGFGISTPEHVRDAIAAGASGAITGSALTSI
IERHTTGTHPEPAQVTDLEALTEEIFAFVKDMKAATR

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=278, Percent_Identity=47.841726618705, Blast_Score=241, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6321412, Length=237, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_CORDI (Q6NEB5)

Other databases:

- EMBL:   BX248361
- RefSeq:   NP_940661.1
- ProteinModelPortal:   Q6NEB5
- SMR:   Q6NEB5
- GeneID:   2649183
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP2361
- NMPDR:   fig|257309.1.peg.2252
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q6NEB5
- ProtClustDB:   PRK13111
- BioCyc:   CDIP257309:DIP2361-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29322; Mature: 29190

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYTKLFSALSAKNEGAFVPFIMLGAPTPEASLAIIRTVVAAGADALELGVPFSDPVAD
CCHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCC
GPTIQRSHLRALDNGATVDSSLELIRQIRKEFPELPIGMLIYGNVAFTRGITQFYQEFAD
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHH
AGVDSILLPDVPVREGEPFIAAAKQAGIDPIFIAPAQASEATLEGVAQHSSGYIYAISRD
CCCCEEECCCCCCCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECC
GVTGTERQSSTRGLDKVVANVKRFGGAPILLGFGISTPEHVRDAIAAGASGAITGSALTS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHH
IIERHTTGTHPEPAQVTDLEALTEEIFAFVKDMKAATR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRYTKLFSALSAKNEGAFVPFIMLGAPTPEASLAIIRTVVAAGADALELGVPFSDPVAD
CHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCC
GPTIQRSHLRALDNGATVDSSLELIRQIRKEFPELPIGMLIYGNVAFTRGITQFYQEFAD
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHH
AGVDSILLPDVPVREGEPFIAAAKQAGIDPIFIAPAQASEATLEGVAQHSSGYIYAISRD
CCCCEEECCCCCCCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECC
GVTGTERQSSTRGLDKVVANVKRFGGAPILLGFGISTPEHVRDAIAAGASGAITGSALTS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHH
IIERHTTGTHPEPAQVTDLEALTEEIFAFVKDMKAATR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910