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The map label for this gene is panB

Identifier: 38234892

GI number: 38234892

Start: 2464011

End: 2464802

Strand: Reverse

Name: panB

Synonym: DIP2358

Alternate gene names: 38234892

Gene position: 2464802-2464011 (Counterclockwise)

Preceding gene: 38234898

Following gene: 38234883

Centisome position: 99.04

GC content: 58.71

Gene sequence:

>792_bases
GTGACCCGTCCCGTCCGACTGCAAGACTTGCGCGACTACAAAAAGAACAACCGTCCGTGGGCCATGCTGACCACCTACGA
CTACTCCACCACTCGCGCATTTGTGGACGCGGGTATCGAGGTTTTCCTCGTAGGAGATTCTGCGGCCAACACAATGCTGG
GATATTCCGGAACTAACCAGGTGAGCTTTGAGGAGATGGTCATGCTCACGGCGGCGGTGGTGCGCGGTGCGGGTCCTGCG
ATGGTGGTGGCTGACCTTCCGTTCGGCACCTACGAGGCGAGTGACGAGCAGGCGGTGCGCAGTGCCACGGAGATGGTTCG
TCGAACTGGTGCTCACGTGGTCAAGCTTGAAGGTGGCGTTCGGATGGCGTCGCGGATTCGCGCTATTACAGATGCAGGGG
TTCCGGTGTGCGCACACCTAGGTTTTACTCCGCAGTCGATGAATCAGCTTTCTGGTTTTAAGGTGCAGCGAGGCGCGGAG
AAGCTGACGGCTGATGTGGATGCTGTCGTGGAGGCGGGTGCAGAGATGGTGGTGTTTGAGATGGTTCCGGCGGATCTTGC
CGCGGAGCTTACTGCCCGCTGCCCGATTCCGGTGATTGGAATTGGCGCCGGCAATGCTACCGACGCCCAGGTGTTGGTGT
GGCACGATATCGCGAATCTTCCTAAAGGTGATCATCGTGCCCGTTTTGTTAAGACGTATGCAGAGGTTGGTTCGCAGCTT
ACTTGCGCGGCGCGGTTCTATCGAAAGGATGTGGAGGCACGTCGCTTCCCCGCTGAGGAGCACACGTTCTAA

Upstream 100 bases:

>100_bases
ACCACCCCCTTGGGTGCCCCATGGAACGTCGTTGTCGGTCATCGCGGACTAGAGTAGTCGCAACTCTTGCTCAACTTTGT
AACTACTGAAAGGTGCCGCT

Downstream 100 bases:

>100_bases
GCGTTGGGCTTTTCGACGTTCACCGCCATGTTGTCTAGCAGTCGGGTGCTTCCTACCTTGACGGCGGTGATGATTCGCGC
GTCGCCGTAGAGTTCATTGC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTRPVRLQDLRDYKKNNRPWAMLTTYDYSTTRAFVDAGIEVFLVGDSAANTMLGYSGTNQVSFEEMVMLTAAVVRGAGPA
MVVADLPFGTYEASDEQAVRSATEMVRRTGAHVVKLEGGVRMASRIRAITDAGVPVCAHLGFTPQSMNQLSGFKVQRGAE
KLTADVDAVVEAGAEMVVFEMVPADLAAELTARCPIPVIGIGAGNATDAQVLVWHDIANLPKGDHRARFVKTYAEVGSQL
TCAARFYRKDVEARRFPAEEHTF

Sequences:

>Translated_263_residues
MTRPVRLQDLRDYKKNNRPWAMLTTYDYSTTRAFVDAGIEVFLVGDSAANTMLGYSGTNQVSFEEMVMLTAAVVRGAGPA
MVVADLPFGTYEASDEQAVRSATEMVRRTGAHVVKLEGGVRMASRIRAITDAGVPVCAHLGFTPQSMNQLSGFKVQRGAE
KLTADVDAVVEAGAEMVVFEMVPADLAAELTARCPIPVIGIGAGNATDAQVLVWHDIANLPKGDHRARFVKTYAEVGSQL
TCAARFYRKDVEARRFPAEEHTF
>Mature_262_residues
TRPVRLQDLRDYKKNNRPWAMLTTYDYSTTRAFVDAGIEVFLVGDSAANTMLGYSGTNQVSFEEMVMLTAAVVRGAGPAM
VVADLPFGTYEASDEQAVRSATEMVRRTGAHVVKLEGGVRMASRIRAITDAGVPVCAHLGFTPQSMNQLSGFKVQRGAEK
LTADVDAVVEAGAEMVVFEMVPADLAAELTARCPIPVIGIGAGNATDAQVLVWHDIANLPKGDHRARFVKTYAEVGSQLT
CAARFYRKDVEARRFPAEEHTF

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=265, Percent_Identity=43.0188679245283, Blast_Score=204, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6319653, Length=269, Percent_Identity=34.2007434944238, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_CORDI (Q6NEB7)

Other databases:

- EMBL:   BX248361
- RefSeq:   NP_940659.1
- HSSP:   P0A5Q8
- ProteinModelPortal:   Q6NEB7
- SMR:   Q6NEB7
- GeneID:   2649181
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP2358
- NMPDR:   fig|257309.1.peg.2250
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- PhylomeDB:   Q6NEB7
- ProtClustDB:   PRK00311
- BioCyc:   CDIP257309:DIP2358-MONOMER
- BRENDA:   2.1.2.11
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28563; Mature: 28432

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: ACT_SITE 180-180 BINDING 85-85 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPVRLQDLRDYKKNNRPWAMLTTYDYSTTRAFVDAGIEVFLVGDSAANTMLGYSGTNQ
CCCCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHCCEEEEEEECCCCCCEECCCCCCC
VSFEEMVMLTAAVVRGAGPAMVVADLPFGTYEASDEQAVRSATEMVRRTGAHVVKLEGGV
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCH
RMASRIRAITDAGVPVCAHLGFTPQSMNQLSGFKVQRGAEKLTADVDAVVEAGAEMVVFE
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
MVPADLAAELTARCPIPVIGIGAGNATDAQVLVWHDIANLPKGDHRARFVKTYAEVGSQL
ECCHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECHHCCCCCCHHHHHHHHHHHHCCCH
TCAARFYRKDVEARRFPAEEHTF
HHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TRPVRLQDLRDYKKNNRPWAMLTTYDYSTTRAFVDAGIEVFLVGDSAANTMLGYSGTNQ
CCCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHCCEEEEEEECCCCCCEECCCCCCC
VSFEEMVMLTAAVVRGAGPAMVVADLPFGTYEASDEQAVRSATEMVRRTGAHVVKLEGGV
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCH
RMASRIRAITDAGVPVCAHLGFTPQSMNQLSGFKVQRGAEKLTADVDAVVEAGAEMVVFE
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
MVPADLAAELTARCPIPVIGIGAGNATDAQVLVWHDIANLPKGDHRARFVKTYAEVGSQL
ECCHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECHHCCCCCCHHHHHHHHHHHHCCCH
TCAARFYRKDVEARRFPAEEHTF
HHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910