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The map label for this gene is trpG [H]

Identifier: 38234887

GI number: 38234887

Start: 2459588

End: 2460232

Strand: Direct

Name: trpG [H]

Synonym: DIP2353

Alternate gene names: 38234887

Gene position: 2459588-2460232 (Clockwise)

Preceding gene: 38234886

Following gene: 38234888

Centisome position: 98.83

GC content: 57.98

Gene sequence:

>645_bases
ATGAAAACTCAGCAAGCCCATGTCGTGCTCCTTGATAACCACGACTCGTTTGTTTATAACTTGGTGGACGCTTTTGCCGT
CGCCGGCTACCACTGCACAGTCCTACGCAATAGCGTCACAGTGACAGAAGTTCTTGACCTAGAACCCGCTCTCATTTGTC
TCTCCCCAGGACCTGGTCACCCCCGCGAAGCCGGCTGCATGATGGACCTCATCGGGGCGTCGATAGGCACCATCCCGATC
CTCGGCATCTGCCTCGGCTTCCAAGCACTCCTCGAACACCACGGTGGGGAAGTCCGCCCCTGCGGCCCCGTCCACGGCAC
CACCGACCTCATGACGCTCACCAACCCAGACCATCCACTGTTCGCAGGACTTAGCATCGACGCCACACCCAACAACAATT
TCCACGGAACACAGGTGCCAATCGCACGCTATCACTCCCTAGGATGCACCGACATCCCACCAAACATGGTGAGCCTAGGA
ACCTGCGACTCCGCCATCGGACCTGTCACCATGGCCGCCGAAACCCACGACGGCGAGGAGCAGACCACCAGCATTGGATT
GCAATTCCACCCCGAATCCGTACTAAGCCCCAGCGGGCCCATCATCCTTGAACGATGCGTCGATAAGCTGCTAGCCAACA
AATAA

Upstream 100 bases:

>100_bases
CTATTCCACAATCAGAGGCCGATGAAACACTCCATAAGGCCTATGCCGTTTTGCATGCCATTGCATTGGCGGCCGGAAAG
GATCTGGAGGTGGTTCGCTA

Downstream 100 bases:

>100_bases
GAAAGACCACAACCATGACAAGCGACAAAACACTCAACAACCTCATCGCCTACCTCGACAACCCACACCCCACCGTCGAA
CAAGCCATTGAAGTATTCAC

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Anthranilate synthase component II; Glutamine amido-transferase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKTQQAHVVLLDNHDSFVYNLVDAFAVAGYHCTVLRNSVTVTEVLDLEPALICLSPGPGHPREAGCMMDLIGASIGTIPI
LGICLGFQALLEHHGGEVRPCGPVHGTTDLMTLTNPDHPLFAGLSIDATPNNNFHGTQVPIARYHSLGCTDIPPNMVSLG
TCDSAIGPVTMAAETHDGEEQTTSIGLQFHPESVLSPSGPIILERCVDKLLANK

Sequences:

>Translated_214_residues
MKTQQAHVVLLDNHDSFVYNLVDAFAVAGYHCTVLRNSVTVTEVLDLEPALICLSPGPGHPREAGCMMDLIGASIGTIPI
LGICLGFQALLEHHGGEVRPCGPVHGTTDLMTLTNPDHPLFAGLSIDATPNNNFHGTQVPIARYHSLGCTDIPPNMVSLG
TCDSAIGPVTMAAETHDGEEQTTSIGLQFHPESVLSPSGPIILERCVDKLLANK
>Mature_214_residues
MKTQQAHVVLLDNHDSFVYNLVDAFAVAGYHCTVLRNSVTVTEVLDLEPALICLSPGPGHPREAGCMMDLIGASIGTIPI
LGICLGFQALLEHHGGEVRPCGPVHGTTDLMTLTNPDHPLFAGLSIDATPNNNFHGTQVPIARYHSLGCTDIPPNMVSLG
TCDSAIGPVTMAAETHDGEEQTTSIGLQFHPESVLSPSGPIILERCVDKLLANK

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=207, Percent_Identity=35.7487922705314, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1789760, Length=202, Percent_Identity=37.1287128712871, Blast_Score=100, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6322638, Length=211, Percent_Identity=33.6492890995261, Blast_Score=104, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324361, Length=225, Percent_Identity=27.1111111111111, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 22677; Mature: 22677

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQQAHVVLLDNHDSFVYNLVDAFAVAGYHCTVLRNSVTVTEVLDLEPALICLSPGPGH
CCCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCEEEEEECCCCEEEEECCCCCC
PREAGCMMDLIGASIGTIPILGICLGFQALLEHHGGEVRPCGPVHGTTDLMTLTNPDHPL
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCE
FAGLSIDATPNNNFHGTQVPIARYHSLGCTDIPPNMVSLGTCDSAIGPVTMAAETHDGEE
EEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCC
QTTSIGLQFHPESVLSPSGPIILERCVDKLLANK
HHEEEEEEECCHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKTQQAHVVLLDNHDSFVYNLVDAFAVAGYHCTVLRNSVTVTEVLDLEPALICLSPGPGH
CCCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCEEEEEECCCCEEEEECCCCCC
PREAGCMMDLIGASIGTIPILGICLGFQALLEHHGGEVRPCGPVHGTTDLMTLTNPDHPL
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCE
FAGLSIDATPNNNFHGTQVPIARYHSLGCTDIPPNMVSLGTCDSAIGPVTMAAETHDGEE
EEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCC
QTTSIGLQFHPESVLSPSGPIILERCVDKLLANK
HHEEEEEEECCHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3808947; 12948626 [H]