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The map label for this gene is liaS [H]

Identifier: 38234804

GI number: 38234804

Start: 2359774

End: 2361153

Strand: Reverse

Name: liaS [H]

Synonym: DIP2268

Alternate gene names: 38234804

Gene position: 2361153-2359774 (Counterclockwise)

Preceding gene: 38234807

Following gene: 38234803

Centisome position: 94.88

GC content: 59.13

Gene sequence:

>1380_bases
GTGGCGGAAGCTTCGTTGGAGCTAGGGGTGTTCTGGGAAGAACAAGCAGATAGCAGCACTGACACGCCCAACGCCGCAGC
AGGCAGTAATGTACGTTTCATGCAGTCAAGCCTAGATCCTGAGAAGCCCTCAGGCACATCACACCTCACCGGCACGGGGC
AGCTCGACGTTTCCCACCACACTGGACGCCCTGGGGCCGCGCTGTACTACCGCTCCATGAACGTGCTCACCAGCGTGTTG
CTGCTGGTATCGCTCGCGGGCGTGGTCAGCTTGGATCGTCGTAGCGCTATCGTCGCGGTCATCCTGAGCGCGCTTTTTGC
CCTCTTCTACGCCGCGGCCGCTGTCAGTCACCGCCGCGCCATGTGGACCAAAGCCATGCTGTTGGTTATTCTCACCGTGC
TGTGGGTCTTTATGCTGCCCATCATTCCAGTGAGTATCTACATGGTCTTCCCACTGTTTTTCCTCTATCTGAGGGTGCTG
CCAGATATTCGCGGCATTATTTTGGTGGTCGGAGCGACGGCGATCGCGATTACCAGCCAATTAGCGCAGCTCACCATTGG
TGCGGTGATGGGGCCGGTGGTATCGGCGCTGGTAGTTATCGCTATCCATTTCGCGTTCGAGGCGATCTGGAAGGGTGCGC
GTGAGCGCGAGGAGCTTATCGACGAACTCCTAGCCACGCGGAATCAGTTGGCAGAAACGGAACGCGCCGCGGGTATCGCC
GCAGAGCGCCAGCGCATCGCCCACGAGATTCACGACACGCTCGCCCAGGGGCTTTCCAGTATCCAAATGTTGCTGCGCGT
TGCCGAGCAAGACATTAAAAAGTCAGGAATGCCGGAAGCTGAGCAACAAGCACCCTTGCATCGCATGGAGCTTGCGCGCT
CGACCGCCGCGGAAAACTTACGCGAAGCTCGTGCCATGATCGCAGCCTTGCAACCTGCAGCGTTGAGCAAAACCTCGCTC
GAAGGGGCATTGAATCGGATCGCGGAGCATGTGGTGGGCCCCGAGATTAGCATTGAGGTCGACGGCGAGGAACGCCAACT
GCCCATGCGCGTTGAGGCAGCCTTGGTGCGTATCGCTCAAGGGGCGCTGGGCAACGTCGCAAAGCATTCGGGCGCCACGC
ATTGCCACCTCACCTTAAGTTACGGGGAAGAAGAAGTGCGTCTCGACGTAGTGGACAACGGCCATGGCTTCGACCCCGAG
CAAGTAGCGCAACGCCCCGCGGGGTTGGGACACGTGGGCATTAGCGCCATGCAACAACGCGCTGCGGAACTAGGCGGGGA
GCTGATTGTGGAGTCGGAACCTGGTCAAGGAACCGCGGTGTCGGTGGCGTTGCCTATAGACATCGAGGAAATTCATTCAA
AAGATCAGGATCAGTGTTAG

Upstream 100 bases:

>100_bases
CGGGCACTCCTCGAACCCCTCGCGGCCCTGGGGTGGCATGGGGAGGGCGTCGAAAGGCAAAGCGGAATCGTGCACCACCG
CGGGGTCGGCGGGGGCAGGA

Downstream 100 bases:

>100_bases
CAATTAGTTGTACAGCTGGGCTAGGTTAGTGAGAAGGTACCTGCACGCGCAAGCGCATAAAAGAAAGGGCGTGGAGAACG
CATGATCCGAGTTTTGCTCG

Product: putative two component system histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MAEASLELGVFWEEQADSSTDTPNAAAGSNVRFMQSSLDPEKPSGTSHLTGTGQLDVSHHTGRPGAALYYRSMNVLTSVL
LLVSLAGVVSLDRRSAIVAVILSALFALFYAAAAVSHRRAMWTKAMLLVILTVLWVFMLPIIPVSIYMVFPLFFLYLRVL
PDIRGIILVVGATAIAITSQLAQLTIGAVMGPVVSALVVIAIHFAFEAIWKGAREREELIDELLATRNQLAETERAAGIA
AERQRIAHEIHDTLAQGLSSIQMLLRVAEQDIKKSGMPEAEQQAPLHRMELARSTAAENLREARAMIAALQPAALSKTSL
EGALNRIAEHVVGPEISIEVDGEERQLPMRVEAALVRIAQGALGNVAKHSGATHCHLTLSYGEEEVRLDVVDNGHGFDPE
QVAQRPAGLGHVGISAMQQRAAELGGELIVESEPGQGTAVSVALPIDIEEIHSKDQDQC

Sequences:

>Translated_459_residues
MAEASLELGVFWEEQADSSTDTPNAAAGSNVRFMQSSLDPEKPSGTSHLTGTGQLDVSHHTGRPGAALYYRSMNVLTSVL
LLVSLAGVVSLDRRSAIVAVILSALFALFYAAAAVSHRRAMWTKAMLLVILTVLWVFMLPIIPVSIYMVFPLFFLYLRVL
PDIRGIILVVGATAIAITSQLAQLTIGAVMGPVVSALVVIAIHFAFEAIWKGAREREELIDELLATRNQLAETERAAGIA
AERQRIAHEIHDTLAQGLSSIQMLLRVAEQDIKKSGMPEAEQQAPLHRMELARSTAAENLREARAMIAALQPAALSKTSL
EGALNRIAEHVVGPEISIEVDGEERQLPMRVEAALVRIAQGALGNVAKHSGATHCHLTLSYGEEEVRLDVVDNGHGFDPE
QVAQRPAGLGHVGISAMQQRAAELGGELIVESEPGQGTAVSVALPIDIEEIHSKDQDQC
>Mature_458_residues
AEASLELGVFWEEQADSSTDTPNAAAGSNVRFMQSSLDPEKPSGTSHLTGTGQLDVSHHTGRPGAALYYRSMNVLTSVLL
LVSLAGVVSLDRRSAIVAVILSALFALFYAAAAVSHRRAMWTKAMLLVILTVLWVFMLPIIPVSIYMVFPLFFLYLRVLP
DIRGIILVVGATAIAITSQLAQLTIGAVMGPVVSALVVIAIHFAFEAIWKGAREREELIDELLATRNQLAETERAAGIAA
ERQRIAHEIHDTLAQGLSSIQMLLRVAEQDIKKSGMPEAEQQAPLHRMELARSTAAENLREARAMIAALQPAALSKTSLE
GALNRIAEHVVGPEISIEVDGEERQLPMRVEAALVRIAQGALGNVAKHSGATHCHLTLSYGEEEVRLDVVDNGHGFDPEQ
VAQRPAGLGHVGISAMQQRAAELGGELIVESEPGQGTAVSVALPIDIEEIHSKDQDQC

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=236, Percent_Identity=28.8135593220339, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49357; Mature: 49226

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEASLELGVFWEEQADSSTDTPNAAAGSNVRFMQSSLDPEKPSGTSHLTGTGQLDVSHH
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEECCC
TGRPGAALYYRSMNVLTSVLLLVSLAGVVSLDRRSAIVAVILSALFALFYAAAAVSHRRA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MWTKAMLLVILTVLWVFMLPIIPVSIYMVFPLFFLYLRVLPDIRGIILVVGATAIAITSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LAQLTIGAVMGPVVSALVVIAIHFAFEAIWKGAREREELIDELLATRNQLAETERAAGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERQRIAHEIHDTLAQGLSSIQMLLRVAEQDIKKSGMPEAEQQAPLHRMELARSTAAENL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHH
REARAMIAALQPAALSKTSLEGALNRIAEHVVGPEISIEVDGEERQLPMRVEAALVRIAQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHH
GALGNVAKHSGATHCHLTLSYGEEEVRLDVVDNGHGFDPEQVAQRPAGLGHVGISAMQQR
HHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHH
AAELGGELIVESEPGQGTAVSVALPIDIEEIHSKDQDQC
HHHCCCEEEEECCCCCCCEEEEEECCCHHHHCCCCCCCC
>Mature Secondary Structure 
AEASLELGVFWEEQADSSTDTPNAAAGSNVRFMQSSLDPEKPSGTSHLTGTGQLDVSHH
CCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEECCC
TGRPGAALYYRSMNVLTSVLLLVSLAGVVSLDRRSAIVAVILSALFALFYAAAAVSHRRA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MWTKAMLLVILTVLWVFMLPIIPVSIYMVFPLFFLYLRVLPDIRGIILVVGATAIAITSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LAQLTIGAVMGPVVSALVVIAIHFAFEAIWKGAREREELIDELLATRNQLAETERAAGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERQRIAHEIHDTLAQGLSSIQMLLRVAEQDIKKSGMPEAEQQAPLHRMELARSTAAENL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHH
REARAMIAALQPAALSKTSLEGALNRIAEHVVGPEISIEVDGEERQLPMRVEAALVRIAQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHH
GALGNVAKHSGATHCHLTLSYGEEEVRLDVVDNGHGFDPEQVAQRPAGLGHVGISAMQQR
HHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHH
AAELGGELIVESEPGQGTAVSVALPIDIEEIHSKDQDQC
HHHCCCEEEEECCCCCCCEEEEEECCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]