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The map label for this gene is liaR [H]

Identifier: 38234803

GI number: 38234803

Start: 2359054

End: 2359692

Strand: Reverse

Name: liaR [H]

Synonym: DIP2267

Alternate gene names: 38234803

Gene position: 2359692-2359054 (Counterclockwise)

Preceding gene: 38234804

Following gene: 38234800

Centisome position: 94.82

GC content: 59.78

Gene sequence:

>639_bases
ATGATCCGAGTTTTGCTCGCTGACGATCACGAAATTGTCCGATTAGGTCTTCGAGCCGTGCTCGAAAGCGCCGAGGACAT
TGAAGTAATCGGTGAAGTAGCCACCGCTGAAGCAGCCATCGCAGCAGCACAAGCAGGCGGTATCGACGTGATCCTCATGG
ATCTGCGCTTCGGCCCAGGCGTGCAGGGGACAAAGCTCACCAGCGGTGCCGACGCAACCGCCGCCATCCGACGCCGGATG
GACAACCCACCAGAGGTGCTCGTGGTGACCAACTATGACACCGACGCGGACATCCTCGGTGCCATTGAAGCCGGAGCCCT
TGGCTACATGCTCAAAGACGCACCCCCAGAGGAACTGCTGGCAGCAGTGCGTTCGGCGGCAGAAGGCGACACCGCACTAT
CACCCACCGTGGCTAACCGCTTGATGTCGCGAGTACGTGCCCCACGAAATTCGCTCACCCCACGCGAACTGGAAGTGCTC
AAGTTGGTTGCTGGTGGCTCTTCCAACCGTGATATTGGGCGCAGGCTGCTGCTGTCTGAGGCAACGGTAAAAAGCCACCT
CGTGCACATCTATGACAAGCTAGGCGTGCGCTCCCGCACCTCGGCTGTGGCTATCGCACGAGAACAAGGCGTGCTGTAG

Upstream 100 bases:

>100_bases
AGATCAGGATCAGTGTTAGCAATTAGTTGTACAGCTGGGCTAGGTTAGTGAGAAGGTACCTGCACGCGCAAGCGCATAAA
AGAAAGGGCGTGGAGAACGC

Downstream 100 bases:

>100_bases
CGAAGCATAAAAAATATCACCGCAGCAGAGCTGGTTGTGCTCCGCGGCGGTGATATTTTTTAGCGCTTGGTGGACTTAAG
GAACGCCTCGATCACATTCG

Product: putative two component system response regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIRVLLADDHEIVRLGLRAVLESAEDIEVIGEVATAEAAIAAAQAGGIDVILMDLRFGPGVQGTKLTSGADATAAIRRRM
DNPPEVLVVTNYDTDADILGAIEAGALGYMLKDAPPEELLAAVRSAAEGDTALSPTVANRLMSRVRAPRNSLTPRELEVL
KLVAGGSSNRDIGRRLLLSEATVKSHLVHIYDKLGVRSRTSAVAIAREQGVL

Sequences:

>Translated_212_residues
MIRVLLADDHEIVRLGLRAVLESAEDIEVIGEVATAEAAIAAAQAGGIDVILMDLRFGPGVQGTKLTSGADATAAIRRRM
DNPPEVLVVTNYDTDADILGAIEAGALGYMLKDAPPEELLAAVRSAAEGDTALSPTVANRLMSRVRAPRNSLTPRELEVL
KLVAGGSSNRDIGRRLLLSEATVKSHLVHIYDKLGVRSRTSAVAIAREQGVL
>Mature_212_residues
MIRVLLADDHEIVRLGLRAVLESAEDIEVIGEVATAEAAIAAAQAGGIDVILMDLRFGPGVQGTKLTSGADATAAIRRRM
DNPPEVLVVTNYDTDADILGAIEAGALGYMLKDAPPEELLAAVRSAAEGDTALSPTVANRLMSRVRAPRNSLTPRELEVL
KLVAGGSSNRDIGRRLLLSEATVKSHLVHIYDKLGVRSRTSAVAIAREQGVL

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=213, Percent_Identity=38.0281690140845, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788222, Length=219, Percent_Identity=30.1369863013699, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1787473, Length=216, Percent_Identity=35.1851851851852, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=32.3232323232323, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786747, Length=210, Percent_Identity=25.7142857142857, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788546, Length=201, Percent_Identity=29.3532338308458, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1788712, Length=210, Percent_Identity=22.8571428571429, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22408; Mature: 22408

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVLLADDHEIVRLGLRAVLESAEDIEVIGEVATAEAAIAAAQAGGIDVILMDLRFGPG
CEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
VQGTKLTSGADATAAIRRRMDNPPEVLVVTNYDTDADILGAIEAGALGYMLKDAPPEELL
CCCCEECCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
AAVRSAAEGDTALSPTVANRLMSRVRAPRNSLTPRELEVLKLVAGGSSNRDIGRRLLLSE
HHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
ATVKSHLVHIYDKLGVRSRTSAVAIAREQGVL
HHHHHHHHHHHHHHCCCCCHHHHEEHHHCCCC
>Mature Secondary Structure
MIRVLLADDHEIVRLGLRAVLESAEDIEVIGEVATAEAAIAAAQAGGIDVILMDLRFGPG
CEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
VQGTKLTSGADATAAIRRRMDNPPEVLVVTNYDTDADILGAIEAGALGYMLKDAPPEELL
CCCCEECCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
AAVRSAAEGDTALSPTVANRLMSRVRAPRNSLTPRELEVLKLVAGGSSNRDIGRRLLLSE
HHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
ATVKSHLVHIYDKLGVRSRTSAVAIAREQGVL
HHHHHHHHHHHHHHCCCCCHHHHEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]