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The map label for this gene is mdh

Identifier: 38234358

GI number: 38234358

Start: 1833151

End: 1834131

Strand: Reverse

Name: mdh

Synonym: DIP1787

Alternate gene names: 38234358

Gene position: 1834131-1833151 (Counterclockwise)

Preceding gene: 38234360

Following gene: 38234357

Centisome position: 73.7

GC content: 55.96

Gene sequence:

>981_bases
ATGACCGAATCAGTAAAGAAGATTGCCGTAACCGGTGCTGCTGGCCAGATCGCCTACTCACTGTTGTGGCGCATTGCTAA
CGGTGACGTGTACGGCAAGAACACTCCAGTTGAATTGCAGCTGCTGGAAATCCCGCAGGCAATTGGCGGCGCAGAAGGTG
TTGCCATGGAGCTTCTTGACTCGGCCTTCCCATTGCTCAAGAACATTGTGGTCACTGATAAAGCAGAAGTTGCTTTTGAC
GGCACCAATGCAGCCTTCCTTGTCGGCGCGAAGCCACGTGGCAAGGGCGAAGAGCGTGCAGATCTGTTGACCGCAAACGG
CAAGATCTTCGGACCACAGGGCAAGGCGCTCAACGACAACGCTGCTGATGACATCCGCGTGCTCGTTGTGGGCAACCCTG
CTAACACCAATGCTTTGATTGCACAGCACGCTGCCAAAGACATCCCAGCAGATCGCTTCAATGCCATGATGCGTCTAGAC
CACAACCGTGGTATCGCTCAACTGTCTGAGAAGCTCGGCCGCGACAAGAATGACATCGAAAAGTTCGTCGTGTGGGGAAA
CCACTCTGCAGGCCAGTTCCCTGACATCACCTACGCAACCATTGGTGGCGAGGCAATCTCCGGTTTGGTTGATCACGATT
GGTACACCGGCGAGTTCATTCCACGTGTTGCTAAGCGCGGCGCTGAGATCATTGAGGTTCGCGGCAAGTCCTCGGCAGCG
TCGGCAGCATCCTCTGCAATCGACCACATGCACGATTGGATCAACGGCACCGACGGCCAGTGGCGTACCGCTGCCATCCC
ATCCGATGGCTCCTATGGTGTCCCTGAGGGCTTGATCTTCGGCTTCCCAACCATCTCTGAGGATGGGCAGTGGAAGATTG
TTCAGGATCTTGAGCTTAGCGACTTCCAGAAGGACGGCATCGCTCGCAACGTCACCGAGCTGGAAGAGGAGCGCGAGGCA
GTCAAGGATCTGCTCGGCTAA

Upstream 100 bases:

>100_bases
TGGTGTTGTTTGACGCAGTTCACAACAAATGAATGCTTGATGTTGTTGTCATGTACGTTGTCGCTGCGATCGCGAGACTG
AAATAAGGAGAGGATTCGAG

Downstream 100 bases:

>100_bases
TCTTTTTCGCACTGTTGTACAACGAGAGCCCATCGCACGCATTGTGGTGGGCTCTCGTTCAGCTTCACATCAAAAGACAG
TTAAGATAGTCCCCATGACT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTESVKKIAVTGAAGQIAYSLLWRIANGDVYGKNTPVELQLLEIPQAIGGAEGVAMELLDSAFPLLKNIVVTDKAEVAFD
GTNAAFLVGAKPRGKGEERADLLTANGKIFGPQGKALNDNAADDIRVLVVGNPANTNALIAQHAAKDIPADRFNAMMRLD
HNRGIAQLSEKLGRDKNDIEKFVVWGNHSAGQFPDITYATIGGEAISGLVDHDWYTGEFIPRVAKRGAEIIEVRGKSSAA
SAASSAIDHMHDWINGTDGQWRTAAIPSDGSYGVPEGLIFGFPTISEDGQWKIVQDLELSDFQKDGIARNVTELEEEREA
VKDLLG

Sequences:

>Translated_326_residues
MTESVKKIAVTGAAGQIAYSLLWRIANGDVYGKNTPVELQLLEIPQAIGGAEGVAMELLDSAFPLLKNIVVTDKAEVAFD
GTNAAFLVGAKPRGKGEERADLLTANGKIFGPQGKALNDNAADDIRVLVVGNPANTNALIAQHAAKDIPADRFNAMMRLD
HNRGIAQLSEKLGRDKNDIEKFVVWGNHSAGQFPDITYATIGGEAISGLVDHDWYTGEFIPRVAKRGAEIIEVRGKSSAA
SAASSAIDHMHDWINGTDGQWRTAAIPSDGSYGVPEGLIFGFPTISEDGQWKIVQDLELSDFQKDGIARNVTELEEEREA
VKDLLG
>Mature_325_residues
TESVKKIAVTGAAGQIAYSLLWRIANGDVYGKNTPVELQLLEIPQAIGGAEGVAMELLDSAFPLLKNIVVTDKAEVAFDG
TNAAFLVGAKPRGKGEERADLLTANGKIFGPQGKALNDNAADDIRVLVVGNPANTNALIAQHAAKDIPADRFNAMMRLDH
NRGIAQLSEKLGRDKNDIEKFVVWGNHSAGQFPDITYATIGGEAISGLVDHDWYTGEFIPRVAKRGAEIIEVRGKSSAAS
AASSAIDHMHDWINGTDGQWRTAAIPSDGSYGVPEGLIFGFPTISEDGQWKIVQDLELSDFQKDGIARNVTELEEEREAV
KDLLG

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=333, Percent_Identity=45.6456456456456, Blast_Score=301, Evalue=6e-82,
Organism=Homo sapiens, GI89886456, Length=327, Percent_Identity=21.1009174311927, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17561064, Length=320, Percent_Identity=53.125, Blast_Score=312, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI32566798, Length=257, Percent_Identity=52.1400778210117, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=51.1764705882353, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24583394, Length=331, Percent_Identity=48.3383685800604, Blast_Score=296, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MDH_CORDI (P61974)

Other databases:

- EMBL:   BX248359
- RefSeq:   NP_940125.1
- ProteinModelPortal:   P61974
- SMR:   P61974
- GeneID:   2649412
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1787
- NMPDR:   fig|257309.1.peg.1716
- HOGENOM:   HBG289884
- OMA:   PQGRAIN
- ProtClustDB:   PRK05442
- BioCyc:   CDIP257309:DIP1787-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 34977; Mature: 34845

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 188-188 BINDING 93-93 BINDING 99-99 BINDING 106-106 BINDING 113-113 BINDING 132-132 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESVKKIAVTGAAGQIAYSLLWRIANGDVYGKNTPVELQLLEIPQAIGGAEGVAMELLD
CCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECHHHHCCCCHHHHHHHH
SAFPLLKNIVVTDKAEVAFDGTNAAFLVGAKPRGKGEERADLLTANGKIFGPQGKALNDN
HHHHHHHHHEEECCCEEEECCCCEEEEEECCCCCCCCCHHHEEEECCEEECCCCCCCCCC
AADDIRVLVVGNPANTNALIAQHAAKDIPADRFNAMMRLDHNRGIAQLSEKLGRDKNDIE
CCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHE
KFVVWGNHSAGQFPDITYATIGGEAISGLVDHDWYTGEFIPRVAKRGAEIIEVRGKSSAA
EEEEECCCCCCCCCCEEEEEECHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCHHH
SAASSAIDHMHDWINGTDGQWRTAAIPSDGSYGVPEGLIFGFPTISEDGQWKIVQDLELS
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCHH
DFQKDGIARNVTELEEEREAVKDLLG
HHHHCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TESVKKIAVTGAAGQIAYSLLWRIANGDVYGKNTPVELQLLEIPQAIGGAEGVAMELLD
CCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECHHHHCCCCHHHHHHHH
SAFPLLKNIVVTDKAEVAFDGTNAAFLVGAKPRGKGEERADLLTANGKIFGPQGKALNDN
HHHHHHHHHEEECCCEEEECCCCEEEEEECCCCCCCCCHHHEEEECCEEECCCCCCCCCC
AADDIRVLVVGNPANTNALIAQHAAKDIPADRFNAMMRLDHNRGIAQLSEKLGRDKNDIE
CCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHE
KFVVWGNHSAGQFPDITYATIGGEAISGLVDHDWYTGEFIPRVAKRGAEIIEVRGKSSAA
EEEEECCCCCCCCCCEEEEEECHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCHHH
SAASSAIDHMHDWINGTDGQWRTAAIPSDGSYGVPEGLIFGFPTISEDGQWKIVQDLELS
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCHH
DFQKDGIARNVTELEEEREAVKDLLG
HHHHCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910