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The map label for this gene is ilvE [H]

Identifier: 38234211

GI number: 38234211

Start: 1669782

End: 1670897

Strand: Reverse

Name: ilvE [H]

Synonym: DIP1636

Alternate gene names: 38234211

Gene position: 1670897-1669782 (Counterclockwise)

Preceding gene: 38234213

Following gene: 38234206

Centisome position: 67.14

GC content: 53.23

Gene sequence:

>1116_bases
TTGAAGTCCATGAGCTCTATTACGTTTAAGAAAATCGATCATCCGAACCCAACTCCAGCAGACAAAGTACAAGAAATTGT
TGCCAACCCCGGCTTTGGCAAGTATTTCACCGACCACATGGTCACTATCGATTGGAACGAAACTGAAGGCTGGCACAATG
CACAGGTACAGCCATATGCGCCAGTGTCCTTGGACCCTGCTGCCTCGGTGTTCCACTACGGACAAGCAATTTTCGAAGGC
CTGAAAGCCTACCGCCATGCCGATGGCACCATCCGTACCTTCCGTCCGGAAGCAAATGCGGCGCGTTTCCAGCGCTCCGC
TCAGCGTTTGGCTATGCCAGAGCTTCCTATCGAGGTCTTTATCGAGTCAATCCAGCAGCTTGTCTCAGTAGACCACGACT
GGGTTCCAGCAGCCGGTGGTGAGGAATCGCTCTATCTGCGCCCATTCATGATCGCTACCGAGACCACGTTGGGTGTTCAT
TCCTCGAAGTCTTACCGCTATTTTGTTATTGCTTCGCCAGCCGGTGCCTATTTCTCTGGTGGCATCAAGCCTGTGTCCGT
ATGGCTGTCTGAAGATTACGTTCGTGCAGCTCCTGGTGGTACTGGTGCGGCTAAGTTCGCAGGTAACTACGCAGCATCGC
TCATCGCACAAACCCAGGCAGCAGAAAAAGGCTGCGATCAGGTTGTATGGCTCGATGCAATCGAGCACAACTACATCGAA
GAGATGGGCGGAATGAACCTCTTCTTCGTCATGGGCAAAGGGGATCAGGCTCACGTGATTACCCCGAAGCTGTCCGGTTC
ACTGCTTCCTGGTGTTACCCGTGATTCTTTGCTTCAGGTTGCTCGCGATCTTGGCTACACCACTGAAGAACGCTTGGTGT
CTAAGGGCGAGTGGGAAGAAACCGCTACGAGCGGAGCTATGAGCGAAGCATTTGCTTGCGGTACTGCGGCCGTGATTACC
CCAGTTGGATCTGTGAAGTCCACCCATGGTGAGTTCTTGGTAAACAACAACGAGGCAGGTTCGATCACCATGCAGCTTCG
TGAGGCACTTACTGGAATCCAGCGCGGCAGCGTTGCTGATACTCACGGATGGATGCACACTCTCGTAGAGGCGTAA

Upstream 100 bases:

>100_bases
AGGTATATACCTCCAAAAGTTGGTAGTTGGCGCAAAAGCCAAGGAAAAATTTTAGTGTTGTCCAGCTTAGCCAATGGCAT
GCGAGGATGTCTGGTGTACG

Downstream 100 bases:

>100_bases
ATTATTTCTCTTCTATAACGAGATTGAGCCGACTCCTTCTGAAAACAGGGGGAGTCGGCTCTGGAGTTTTGCGGTTTTAA
GCTACTATGCCCACGCCTAC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKSMSSITFKKIDHPNPTPADKVQEIVANPGFGKYFTDHMVTIDWNETEGWHNAQVQPYAPVSLDPAASVFHYGQAIFEG
LKAYRHADGTIRTFRPEANAARFQRSAQRLAMPELPIEVFIESIQQLVSVDHDWVPAAGGEESLYLRPFMIATETTLGVH
SSKSYRYFVIASPAGAYFSGGIKPVSVWLSEDYVRAAPGGTGAAKFAGNYAASLIAQTQAAEKGCDQVVWLDAIEHNYIE
EMGGMNLFFVMGKGDQAHVITPKLSGSLLPGVTRDSLLQVARDLGYTTEERLVSKGEWEETATSGAMSEAFACGTAAVIT
PVGSVKSTHGEFLVNNNEAGSITMQLREALTGIQRGSVADTHGWMHTLVEA

Sequences:

>Translated_371_residues
MKSMSSITFKKIDHPNPTPADKVQEIVANPGFGKYFTDHMVTIDWNETEGWHNAQVQPYAPVSLDPAASVFHYGQAIFEG
LKAYRHADGTIRTFRPEANAARFQRSAQRLAMPELPIEVFIESIQQLVSVDHDWVPAAGGEESLYLRPFMIATETTLGVH
SSKSYRYFVIASPAGAYFSGGIKPVSVWLSEDYVRAAPGGTGAAKFAGNYAASLIAQTQAAEKGCDQVVWLDAIEHNYIE
EMGGMNLFFVMGKGDQAHVITPKLSGSLLPGVTRDSLLQVARDLGYTTEERLVSKGEWEETATSGAMSEAFACGTAAVIT
PVGSVKSTHGEFLVNNNEAGSITMQLREALTGIQRGSVADTHGWMHTLVEA
>Mature_371_residues
MKSMSSITFKKIDHPNPTPADKVQEIVANPGFGKYFTDHMVTIDWNETEGWHNAQVQPYAPVSLDPAASVFHYGQAIFEG
LKAYRHADGTIRTFRPEANAARFQRSAQRLAMPELPIEVFIESIQQLVSVDHDWVPAAGGEESLYLRPFMIATETTLGVH
SSKSYRYFVIASPAGAYFSGGIKPVSVWLSEDYVRAAPGGTGAAKFAGNYAASLIAQTQAAEKGCDQVVWLDAIEHNYIE
EMGGMNLFFVMGKGDQAHVITPKLSGSLLPGVTRDSLLQVARDLGYTTEERLVSKGEWEETATSGAMSEAFACGTAAVIT
PVGSVKSTHGEFLVNNNEAGSITMQLREALTGIQRGSVADTHGWMHTLVEA

Specific function: Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=345, Percent_Identity=40, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI38176287, Length=345, Percent_Identity=40, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI296010904, Length=345, Percent_Identity=40, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI50658084, Length=356, Percent_Identity=37.3595505617978, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI258614015, Length=293, Percent_Identity=37.542662116041, Blast_Score=194, Evalue=8e-50,
Organism=Homo sapiens, GI296010902, Length=345, Percent_Identity=34.7826086956522, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI296010900, Length=345, Percent_Identity=34.7826086956522, Blast_Score=194, Evalue=2e-49,
Organism=Escherichia coli, GI48994963, Length=324, Percent_Identity=30.5555555555556, Blast_Score=121, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17568601, Length=297, Percent_Identity=41.4141414141414, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17565728, Length=360, Percent_Identity=35, Blast_Score=200, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6322002, Length=376, Percent_Identity=38.031914893617, Blast_Score=247, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6322608, Length=334, Percent_Identity=38.622754491018, Blast_Score=237, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24641779, Length=368, Percent_Identity=38.5869565217391, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 40266; Mature: 40266

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSMSSITFKKIDHPNPTPADKVQEIVANPGFGKYFTDHMVTIDWNETEGWHNAQVQPYA
CCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCEECCCEEEEECCCCCCCCCCCCCCCC
PVSLDPAASVFHYGQAIFEGLKAYRHADGTIRTFRPEANAARFQRSAQRLAMPELPIEVF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHH
IESIQQLVSVDHDWVPAAGGEESLYLRPFMIATETTLGVHSSKSYRYFVIASPAGAYFSG
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCEECCCCCCCEEEEEEECCCCCHHCC
GIKPVSVWLSEDYVRAAPGGTGAAKFAGNYAASLIAQTQAAEKGCDQVVWLDAIEHNYIE
CCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
EMGGMNLFFVMGKGDQAHVITPKLSGSLLPGVTRDSLLQVARDLGYTTEERLVSKGEWEE
HHCCCEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCHH
TATSGAMSEAFACGTAAVITPVGSVKSTHGEFLVNNNEAGSITMQLREALTGIQRGSVAD
HHHHHHHHHHHHCCCHHEEECCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHCCCCCCC
THGWMHTLVEA
HHHHHHHHHCC
>Mature Secondary Structure
MKSMSSITFKKIDHPNPTPADKVQEIVANPGFGKYFTDHMVTIDWNETEGWHNAQVQPYA
CCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCEECCCEEEEECCCCCCCCCCCCCCCC
PVSLDPAASVFHYGQAIFEGLKAYRHADGTIRTFRPEANAARFQRSAQRLAMPELPIEVF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHH
IESIQQLVSVDHDWVPAAGGEESLYLRPFMIATETTLGVHSSKSYRYFVIASPAGAYFSG
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCEECCCCCCCEEEEEEECCCCCHHCC
GIKPVSVWLSEDYVRAAPGGTGAAKFAGNYAASLIAQTQAAEKGCDQVVWLDAIEHNYIE
CCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
EMGGMNLFFVMGKGDQAHVITPKLSGSLLPGVTRDSLLQVARDLGYTTEERLVSKGEWEE
HHCCCEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCHH
TATSGAMSEAFACGTAAVITPVGSVKSTHGEFLVNNNEAGSITMQLREALTGIQRGSVAD
HHHHHHHHHHHHCCCHHEEECCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHCCCCCCC
THGWMHTLVEA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA