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The map label for this gene is argG

Identifier: 38233763

GI number: 38233763

Start: 1165505

End: 1166704

Strand: Direct

Name: argG

Synonym: DIP1173

Alternate gene names: 38233763

Gene position: 1165505-1166704 (Clockwise)

Preceding gene: 38233762

Following gene: 38233764

Centisome position: 46.83

GC content: 49.58

Gene sequence:

>1200_bases
ATGACCAATCGTGTCGTCCTCGCCTATTCAGGTGGACTCGATACCTCCGTTGCAATTCCATACCTGGCTAAGATGACTGG
TGGCGAAGTTGTCGCAGTTTCTTTGGATCTTGGCCAAGGCGGAGAGGACATGGAGTCCGTTCGTCAGCGTGCACTTGATT
GTGGTGCGGTTGAATCTATTGTCATTGACGCTAAAGATGAGTTTGCCAATGATTACTGCCTGCCGACTATCAAAGCAAAC
GGCATGTACATGAAGCAGTACCCTTTGGTTTCTGCAATCTCTCGTCCACTCATCGTGAAGCACTTGGTAGAAGCCGCTAA
AAAACATGGTGGTACCCATGTTTCCCATGGTTGTACTGGTAAAGGAAACGATCAGGTTCGATTTGAGGTAGGTTTCCGCA
ACCTTGCTCCAGAACTGCAGATCATTGCCCCAGCCCGTGATTACGCATGGACTCGCGATAAGGCGATTGCTTTTGCTGAA
GAGATTAATCTACCTATCGAGCAATCCAAGAAGTCGCCATTCTCCATCGATCAAAATGTTTGGGGACGTGCCGTAGAGAC
CGGTTTCCTCGAGGATCTCTGGAACCCGCCGACAAAAGATCTATACTCCTACACTGAAGATCCAGCTCTCGGAAATGCTC
CTGATGAGATCATCATTAGCTTCAAAGCTGGTGTTCCGGTAGCAATCGATGGACGTCCAGTCACCGTACTTGAAGCAATT
GAAGAAATGAACCGTCGTGCAGGTGCACAAGGCATTGGCCGTCTGGACATGGTGGAAGATCGTCTCGTTGGTATTAAATC
TCGCGAGGTCTACGAAGCTCCAGGCGCAATTGCACTGATTACAGCACACCAAGCTATGGAAGATGTGACCATTGAGCGTG
AGCTTGCACGTTACAAGCGCGGAATCGACGCTCGTTGGTCTGAAGAGGTGTACGACGGATTGTGGTACGCGCCACTCAAG
CGTTCTTTGGATGCGTTCATTGAAAATACTCAAGAGCACGTAACAGGTGACATTCGCATGGTCATGCATGCTGGTAAGTG
CACCGTAAACGGACGTCGTTCTGAGCATTCGCTTTACGACTTCGACTTGGCTACTTATGACACCGGTGATACCTTCGACC
AGACCTTGGCTAAGGGCTTTGTGGAACTTCACGGTTTGTCATCCAAGATCTCGAACAAGCGTGACCGTGAGGCACAATAG

Upstream 100 bases:

>100_bases
GCGTAAACTATCCGTTTGACTTAATATATGCAGAATTACTTGGTGCTGTAGACATCTTGAGCTTTCTGTATTACAAAAGC
TTTATTCGAAGGAGAAAACC

Downstream 100 bases:

>100_bases
TGCAAAAACACGGTACTAATGAAGGCGCGCTTTGGGGCGGCCGCTTCTCTGGCGGCCCTTCAGAGGCGATGTTCGCGTTA
TCTGTATCTACGCACTTTGA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTNRVVLAYSGGLDTSVAIPYLAKMTGGEVVAVSLDLGQGGEDMESVRQRALDCGAVESIVIDAKDEFANDYCLPTIKAN
GMYMKQYPLVSAISRPLIVKHLVEAAKKHGGTHVSHGCTGKGNDQVRFEVGFRNLAPELQIIAPARDYAWTRDKAIAFAE
EINLPIEQSKKSPFSIDQNVWGRAVETGFLEDLWNPPTKDLYSYTEDPALGNAPDEIIISFKAGVPVAIDGRPVTVLEAI
EEMNRRAGAQGIGRLDMVEDRLVGIKSREVYEAPGAIALITAHQAMEDVTIERELARYKRGIDARWSEEVYDGLWYAPLK
RSLDAFIENTQEHVTGDIRMVMHAGKCTVNGRRSEHSLYDFDLATYDTGDTFDQTLAKGFVELHGLSSKISNKRDREAQ

Sequences:

>Translated_399_residues
MTNRVVLAYSGGLDTSVAIPYLAKMTGGEVVAVSLDLGQGGEDMESVRQRALDCGAVESIVIDAKDEFANDYCLPTIKAN
GMYMKQYPLVSAISRPLIVKHLVEAAKKHGGTHVSHGCTGKGNDQVRFEVGFRNLAPELQIIAPARDYAWTRDKAIAFAE
EINLPIEQSKKSPFSIDQNVWGRAVETGFLEDLWNPPTKDLYSYTEDPALGNAPDEIIISFKAGVPVAIDGRPVTVLEAI
EEMNRRAGAQGIGRLDMVEDRLVGIKSREVYEAPGAIALITAHQAMEDVTIERELARYKRGIDARWSEEVYDGLWYAPLK
RSLDAFIENTQEHVTGDIRMVMHAGKCTVNGRRSEHSLYDFDLATYDTGDTFDQTLAKGFVELHGLSSKISNKRDREAQ
>Mature_398_residues
TNRVVLAYSGGLDTSVAIPYLAKMTGGEVVAVSLDLGQGGEDMESVRQRALDCGAVESIVIDAKDEFANDYCLPTIKANG
MYMKQYPLVSAISRPLIVKHLVEAAKKHGGTHVSHGCTGKGNDQVRFEVGFRNLAPELQIIAPARDYAWTRDKAIAFAEE
INLPIEQSKKSPFSIDQNVWGRAVETGFLEDLWNPPTKDLYSYTEDPALGNAPDEIIISFKAGVPVAIDGRPVTVLEAIE
EMNRRAGAQGIGRLDMVEDRLVGIKSREVYEAPGAIALITAHQAMEDVTIERELARYKRGIDARWSEEVYDGLWYAPLKR
SLDAFIENTQEHVTGDIRMVMHAGKCTVNGRRSEHSLYDFDLATYDTGDTFDQTLAKGFVELHGLSSKISNKRDREAQ

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=408, Percent_Identity=37.7450980392157, Blast_Score=258, Evalue=1e-68,
Organism=Homo sapiens, GI16950633, Length=408, Percent_Identity=37.7450980392157, Blast_Score=258, Evalue=1e-68,
Organism=Escherichia coli, GI1789563, Length=397, Percent_Identity=27.2040302267003, Blast_Score=102, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324514, Length=409, Percent_Identity=33.0073349633252, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI21358151, Length=398, Percent_Identity=35.427135678392, Blast_Score=242, Evalue=4e-64,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_CORDI (P61521)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939530.1
- ProteinModelPortal:   P61521
- SMR:   P61521
- GeneID:   2649725
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1173
- NMPDR:   fig|257309.1.peg.1121
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- BioCyc:   CDIP257309:DIP1173-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44082; Mature: 43951

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 87-87 BINDING 117-117 BINDING 119-119 BINDING 123-123 BINDING 123-123 BINDING 124-124 BINDING 127-127 BINDING 175-175 BINDING 259-259 BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRVVLAYSGGLDTSVAIPYLAKMTGGEVVAVSLDLGQGGEDMESVRQRALDCGAVESI
CCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHEE
VIDAKDEFANDYCLPTIKANGMYMKQYPLVSAISRPLIVKHLVEAAKKHGGTHVSHGCTG
EEECCHHHCCCCCCEEEECCCEEEECCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCC
KGNDQVRFEVGFRNLAPELQIIAPARDYAWTRDKAIAFAEEINLPIEQSKKSPFSIDQNV
CCCCEEEEEECCCCCCCCEEEEECCHHCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCHHH
WGRAVETGFLEDLWNPPTKDLYSYTEDPALGNAPDEIIISFKAGVPVAIDGRPVTVLEAI
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCEEEEEECCCCEEECCCCHHHHHHH
EEMNRRAGAQGIGRLDMVEDRLVGIKSREVYEAPGAIALITAHQAMEDVTIERELARYKR
HHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHC
GIDARWSEEVYDGLWYAPLKRSLDAFIENTQEHVTGDIRMVMHAGKCTVNGRRSEHSLYD
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCCCEEE
FDLATYDTGDTFDQTLAKGFVELHGLSSKISNKRDREAQ
EEEEECCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
>Mature Secondary Structure 
TNRVVLAYSGGLDTSVAIPYLAKMTGGEVVAVSLDLGQGGEDMESVRQRALDCGAVESI
CCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCHHEE
VIDAKDEFANDYCLPTIKANGMYMKQYPLVSAISRPLIVKHLVEAAKKHGGTHVSHGCTG
EEECCHHHCCCCCCEEEECCCEEEECCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCC
KGNDQVRFEVGFRNLAPELQIIAPARDYAWTRDKAIAFAEEINLPIEQSKKSPFSIDQNV
CCCCEEEEEECCCCCCCCEEEEECCHHCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCHHH
WGRAVETGFLEDLWNPPTKDLYSYTEDPALGNAPDEIIISFKAGVPVAIDGRPVTVLEAI
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCEEEEEECCCCEEECCCCHHHHHHH
EEMNRRAGAQGIGRLDMVEDRLVGIKSREVYEAPGAIALITAHQAMEDVTIERELARYKR
HHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHC
GIDARWSEEVYDGLWYAPLKRSLDAFIENTQEHVTGDIRMVMHAGKCTVNGRRSEHSLYD
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCCCEEE
FDLATYDTGDTFDQTLAKGFVELHGLSSKISNKRDREAQ
EEEEECCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910