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The map label for this gene is leuC

Identifier: 38233717

GI number: 38233717

Start: 1111988

End: 1113430

Strand: Direct

Name: leuC

Synonym: DIP1127

Alternate gene names: 38233717

Gene position: 1111988-1113430 (Clockwise)

Preceding gene: 38233715

Following gene: 38233718

Centisome position: 44.68

GC content: 57.38

Gene sequence:

>1443_bases
ATGACCAGCCCCATGACATCGAAGGATTCAAAACTCACGCTCGCTGAAAAAGTCTGGCGTGACCACGTCGTTTCTCAAGG
AGAAGGCGACCAACCAGATCTTATCTTCATTGACCTTCAATTACTGCATGAAGTCACCTCACCTCAGGCTTTCGACGGTC
TGCGCATGGCCGGACGTACACTGCGACACCCAGAACTGCACCTAGCCACCGAAGACCACAACGTTCCCACCGAGGGAATC
CACAACGGCTCCCTGCTAGAAATCAACGACCTTGTCTCACGCACCCAGGTAGAAACCCTGCGGAAAAACTGCGAAGAATT
TGGAGTGCGCCTCCACGCCATGGGCGATAAAAAACAAGGCATCGTGCACCAGGTAGGCCCACAATTAGGTGCCACCCAGC
CAGGCATGACCATCGTGTGCGGCGACTCGCACACCTCCACGCACGGAGCCTTCGGTGCTATGGCCTTTGGTATCGGAACC
TCTGAGGTAGAACATGTGATGGCAACACAAACGCTGTCGCTAAAACCATTTAAGACCATGGCCATTAATGTCACCGGCGA
GCTGCAACCAGGGGTCACCGCAAAAGACCTCATTTTGGCGGTCATCGCAACCATCGGCACCGGCGGCGGACAAGGCCACG
TGATCGAATACCGCGGTGAAGCAATCGAAAAACTGTCCATGGAAGCGCGCATGACAGTCTGCAACATGTCCATCGAAGCA
GGTGCTCGCGCCGGCATGATCGCCCCCGACGAGACCACATTCGACTACATCAAAGGCCGCGAAATGGCGCCCACCGGCCA
AGACTGGGACGACGCCGTGGCCTACTGGAAGACCCTGCCCACCGACGAAGGAGCCGAATTCGATACCGAAATCACTATCG
ACGGCTCCGCGATCACACCATTTATCACATGGGGAACAAACCCAGGCCAAGGCCTACCGCTGTCAAGCGTCGTGCCCTCG
CCAGAGGACTTCCCAGGAGATAACGAAAAAGTCGCAGCCGAAAAAGCACTCGCCTACATGGGACTAACCCCAGGAACCCC
ACTACGCGACATTGCCATCGACACAGTCTTCCTCGGATCCTGCACCAACGCCCGCATGGATGACCTCCGCATTGCAGCCG
ACATCCTGCGCGGCCGAAGCATCGCCGACTCCGTGCGCATGATGGTCGTCCCATCCTCAACCATGATTAAAGAGCAAGCA
GAGGCAGAAGGCCTAGACAAGATCTTCATCGAAGCCGGCGCACAATGGCGTACCGCAGGATGCTCCATGTGTCTAGGAAT
GAACCCCGATCAACTCACCCCAGGCGAGCGCTGTGCATCCACCTCGAACCGCAACTTCGAAGGCCGCCAAGGCCCAGGCG
GACGCACCCACTTGGTATCACCAGCAGTTGCCGCCGCAACCGCAATCAAGGGAACCCTGGCAAGCCCCGCCGATCTGGAC
TAA

Upstream 100 bases:

>100_bases
ATCTTTCCCATAAGATGAATATTAGCATTCCATTGCGCGGGATTCACCTGTGGGTCGTTTGATGTAGTGGAAAATTTATG
TAGAAGAGGTGATTCGATTC

Downstream 100 bases:

>100_bases
GGAAGAAAACAATGGAAAAATTCACCACCCACACCGGTGTCGGCGTACCGCTGACCCGCTCCAACGTCGACACCGACCAG
ATCATCCCAGCCGTCTACCT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTSPMTSKDSKLTLAEKVWRDHVVSQGEGDQPDLIFIDLQLLHEVTSPQAFDGLRMAGRTLRHPELHLATEDHNVPTEGI
HNGSLLEINDLVSRTQVETLRKNCEEFGVRLHAMGDKKQGIVHQVGPQLGATQPGMTIVCGDSHTSTHGAFGAMAFGIGT
SEVEHVMATQTLSLKPFKTMAINVTGELQPGVTAKDLILAVIATIGTGGGQGHVIEYRGEAIEKLSMEARMTVCNMSIEA
GARAGMIAPDETTFDYIKGREMAPTGQDWDDAVAYWKTLPTDEGAEFDTEITIDGSAITPFITWGTNPGQGLPLSSVVPS
PEDFPGDNEKVAAEKALAYMGLTPGTPLRDIAIDTVFLGSCTNARMDDLRIAADILRGRSIADSVRMMVVPSSTMIKEQA
EAEGLDKIFIEAGAQWRTAGCSMCLGMNPDQLTPGERCASTSNRNFEGRQGPGGRTHLVSPAVAAATAIKGTLASPADLD

Sequences:

>Translated_480_residues
MTSPMTSKDSKLTLAEKVWRDHVVSQGEGDQPDLIFIDLQLLHEVTSPQAFDGLRMAGRTLRHPELHLATEDHNVPTEGI
HNGSLLEINDLVSRTQVETLRKNCEEFGVRLHAMGDKKQGIVHQVGPQLGATQPGMTIVCGDSHTSTHGAFGAMAFGIGT
SEVEHVMATQTLSLKPFKTMAINVTGELQPGVTAKDLILAVIATIGTGGGQGHVIEYRGEAIEKLSMEARMTVCNMSIEA
GARAGMIAPDETTFDYIKGREMAPTGQDWDDAVAYWKTLPTDEGAEFDTEITIDGSAITPFITWGTNPGQGLPLSSVVPS
PEDFPGDNEKVAAEKALAYMGLTPGTPLRDIAIDTVFLGSCTNARMDDLRIAADILRGRSIADSVRMMVVPSSTMIKEQA
EAEGLDKIFIEAGAQWRTAGCSMCLGMNPDQLTPGERCASTSNRNFEGRQGPGGRTHLVSPAVAAATAIKGTLASPADLD
>Mature_479_residues
TSPMTSKDSKLTLAEKVWRDHVVSQGEGDQPDLIFIDLQLLHEVTSPQAFDGLRMAGRTLRHPELHLATEDHNVPTEGIH
NGSLLEINDLVSRTQVETLRKNCEEFGVRLHAMGDKKQGIVHQVGPQLGATQPGMTIVCGDSHTSTHGAFGAMAFGIGTS
EVEHVMATQTLSLKPFKTMAINVTGELQPGVTAKDLILAVIATIGTGGGQGHVIEYRGEAIEKLSMEARMTVCNMSIEAG
ARAGMIAPDETTFDYIKGREMAPTGQDWDDAVAYWKTLPTDEGAEFDTEITIDGSAITPFITWGTNPGQGLPLSSVVPSP
EDFPGDNEKVAAEKALAYMGLTPGTPLRDIAIDTVFLGSCTNARMDDLRIAADILRGRSIADSVRMMVVPSSTMIKEQAE
AEGLDKIFIEAGAQWRTAGCSMCLGMNPDQLTPGERCASTSNRNFEGRQGPGGRTHLVSPAVAAATAIKGTLASPADLD

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=373, Percent_Identity=28.1501340482574, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI8659555, Length=447, Percent_Identity=23.9373601789709, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI41352693, Length=372, Percent_Identity=25.8064516129032, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786259, Length=462, Percent_Identity=61.6883116883117, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1787531, Length=363, Percent_Identity=27.2727272727273, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081781, Length=375, Percent_Identity=27.4666666666667, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI2367097, Length=368, Percent_Identity=25.8152173913043, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=365, Percent_Identity=29.8630136986301, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=379, Percent_Identity=29.5514511873351, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=319, Percent_Identity=29.4670846394984, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=464, Percent_Identity=25, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=452, Percent_Identity=60.6194690265487, Blast_Score=556, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6320440, Length=491, Percent_Identity=26.0692464358452, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=363, Percent_Identity=27.8236914600551, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=394, Percent_Identity=27.1573604060914, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI281365315, Length=390, Percent_Identity=27.1794871794872, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI17864292, Length=390, Percent_Identity=27.1794871794872, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161076999, Length=390, Percent_Identity=27.1794871794872, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI28571643, Length=485, Percent_Identity=26.8041237113402, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24645686, Length=368, Percent_Identity=26.0869565217391, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137564, Length=369, Percent_Identity=25.7452574525745, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_CORDI (Q6NHL0)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939484.1
- ProteinModelPortal:   Q6NHL0
- GeneID:   2649929
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1127
- NMPDR:   fig|257309.1.peg.1075
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   Q6NHL0
- ProtClustDB:   PRK05478
- BioCyc:   CDIP257309:DIP1127-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51239; Mature: 51107

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPMTSKDSKLTLAEKVWRDHVVSQGEGDQPDLIFIDLQLLHEVTSPQAFDGLRMAGRT
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHHCCC
LRHPELHLATEDHNVPTEGIHNGSLLEINDLVSRTQVETLRKNCEEFGVRLHAMGDKKQG
CCCCCEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCC
IVHQVGPQLGATQPGMTIVCGDSHTSTHGAFGAMAFGIGTSEVEHVMATQTLSLKPFKTM
HHHHCCCCCCCCCCCCEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHEECCCCCEEE
AINVTGELQPGVTAKDLILAVIATIGTGGGQGHVIEYRGEAIEKLSMEARMTVCNMSIEA
EEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHEEEEECCHHC
GARAGMIAPDETTFDYIKGREMAPTGQDWDDAVAYWKTLPTDEGAEFDTEITIDGSAITP
CCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCE
FITWGTNPGQGLPLSSVVPSPEDFPGDNEKVAAEKALAYMGLTPGTPLRDIAIDTVFLGS
EEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHEEEEEEECC
CTNARMDDLRIAADILRGRSIADSVRMMVVPSSTMIKEQAEAEGLDKIFIEAGAQWRTAG
CCCCCHHHHHHHHHHHHCCCHHCCEEEEEECCHHHHHHHHHHCCCCEEEECCCCCEECCC
CSMCLGMNPDQLTPGERCASTSNRNFEGRQGPGGRTHLVSPAVAAATAIKGTLASPADLD
CEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TSPMTSKDSKLTLAEKVWRDHVVSQGEGDQPDLIFIDLQLLHEVTSPQAFDGLRMAGRT
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHHCCC
LRHPELHLATEDHNVPTEGIHNGSLLEINDLVSRTQVETLRKNCEEFGVRLHAMGDKKQG
CCCCCEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCC
IVHQVGPQLGATQPGMTIVCGDSHTSTHGAFGAMAFGIGTSEVEHVMATQTLSLKPFKTM
HHHHCCCCCCCCCCCCEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHEECCCCCEEE
AINVTGELQPGVTAKDLILAVIATIGTGGGQGHVIEYRGEAIEKLSMEARMTVCNMSIEA
EEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHEEEEECCHHC
GARAGMIAPDETTFDYIKGREMAPTGQDWDDAVAYWKTLPTDEGAEFDTEITIDGSAITP
CCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCE
FITWGTNPGQGLPLSSVVPSPEDFPGDNEKVAAEKALAYMGLTPGTPLRDIAIDTVFLGS
EEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHEEEEEEECC
CTNARMDDLRIAADILRGRSIADSVRMMVVPSSTMIKEQAEAEGLDKIFIEAGAQWRTAG
CCCCCHHHHHHHHHHHHCCCHHCCEEEEEECCHHHHHHHHHHCCCCEEEECCCCCEECCC
CSMCLGMNPDQLTPGERCASTSNRNFEGRQGPGGRTHLVSPAVAAATAIKGTLASPADLD
CEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910