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The map label for this gene is leuD

Identifier: 38233718

GI number: 38233718

Start: 1113442

End: 1114032

Strand: Direct

Name: leuD

Synonym: DIP1128

Alternate gene names: 38233718

Gene position: 1113442-1114032 (Clockwise)

Preceding gene: 38233717

Following gene: 38233720

Centisome position: 44.74

GC content: 56.35

Gene sequence:

>591_bases
ATGGAAAAATTCACCACCCACACCGGTGTCGGCGTACCGCTGACCCGCTCCAACGTCGACACCGACCAGATCATCCCAGC
CGTCTACCTCAAACGCGTCACCCGCACTGGATTCGAAGACGGACTCTTTAATAACTGGCGCACCAAGGACCCCAACTTTG
TCCTTAACAACGAAGCCTACCGCAATGGATCCGTCCTCGTGGCTGGACCAGACTTTGGCACCGGATCATCACGCGAACAC
GCAGTATGGGCACTCAAAGACTACGGATTCGCCGTCGTCCTCTCATCACGATTCGCCGACATCTTCCGCGGAAACGCCGG
AAAAGCCGGATTACTGGCAGCCCAAATGGAACAAACCGACATCGAGCTGCTATGGAAACAACTCGAACAAACACCCGGCG
CGCAGGTCACCGTAAGCCTCGAAGAACGCACCGTCACCTGCGAAGGAAACGTCTACCCATTCTTCGTCGACGACTACACA
CGGTGGCGCCTCATGGAAGGCCTTGACGACGTAGGACTCACCCTGCGCAAAGAAGCCGAGATTGCAGCCTTTGAAGCACG
CCGGCCATCCTTCAAACCGGTAACGCAATAA

Upstream 100 bases:

>100_bases
CAGGCGGACGCACCCACTTGGTATCACCAGCAGTTGCCGCCGCAACCGCAATCAAGGGAACCCTGGCAAGCCCCGCCGAT
CTGGACTAAGGAAGAAAACA

Downstream 100 bases:

>100_bases
CACCTCAGCCGCAAAGAAAAGTGGGGCTTCCTAGAGCGAACAACTCTACGAGGCCCCACAACTATGCCAGGAAAGCGATT
ACTTCACGCCCAAGGGGCTC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MEKFTTHTGVGVPLTRSNVDTDQIIPAVYLKRVTRTGFEDGLFNNWRTKDPNFVLNNEAYRNGSVLVAGPDFGTGSSREH
AVWALKDYGFAVVLSSRFADIFRGNAGKAGLLAAQMEQTDIELLWKQLEQTPGAQVTVSLEERTVTCEGNVYPFFVDDYT
RWRLMEGLDDVGLTLRKEAEIAAFEARRPSFKPVTQ

Sequences:

>Translated_196_residues
MEKFTTHTGVGVPLTRSNVDTDQIIPAVYLKRVTRTGFEDGLFNNWRTKDPNFVLNNEAYRNGSVLVAGPDFGTGSSREH
AVWALKDYGFAVVLSSRFADIFRGNAGKAGLLAAQMEQTDIELLWKQLEQTPGAQVTVSLEERTVTCEGNVYPFFVDDYT
RWRLMEGLDDVGLTLRKEAEIAAFEARRPSFKPVTQ
>Mature_196_residues
MEKFTTHTGVGVPLTRSNVDTDQIIPAVYLKRVTRTGFEDGLFNNWRTKDPNFVLNNEAYRNGSVLVAGPDFGTGSSREH
AVWALKDYGFAVVLSSRFADIFRGNAGKAGLLAAQMEQTDIELLWKQLEQTPGAQVTVSLEERTVTCEGNVYPFFVDDYT
RWRLMEGLDDVGLTLRKEAEIAAFEARRPSFKPVTQ

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=46.7005076142132, Blast_Score=183, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6321429, Length=202, Percent_Identity=39.1089108910891, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_CORDI (Q6NHK9)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939485.1
- ProteinModelPortal:   Q6NHK9
- SMR:   Q6NHK9
- GeneID:   2649930
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1128
- NMPDR:   fig|257309.1.peg.1076
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   Q6NHK9
- ProtClustDB:   PRK01641
- BioCyc:   CDIP257309:DIP1128-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21957; Mature: 21957

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFTTHTGVGVPLTRSNVDTDQIIPAVYLKRVTRTGFEDGLFNNWRTKDPNFVLNNEAY
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCC
RNGSVLVAGPDFGTGSSREHAVWALKDYGFAVVLSSRFADIFRGNAGKAGLLAAQMEQTD
CCCEEEEECCCCCCCCCCCCEEEEECCCCEEEEEHHHHHHHHCCCCCCCCEEEEHHHHHH
IELLWKQLEQTPGAQVTVSLEERTVTCEGNVYPFFVDDYTRWRLMEGLDDVGLTLRKEAE
HHHHHHHHHHCCCCEEEEEEECEEEEECCCEEEEEECCCHHHHHHHCCCCCCEEEECCCC
IAAFEARRPSFKPVTQ
EEEEECCCCCCCCCCC
>Mature Secondary Structure
MEKFTTHTGVGVPLTRSNVDTDQIIPAVYLKRVTRTGFEDGLFNNWRTKDPNFVLNNEAY
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCC
RNGSVLVAGPDFGTGSSREHAVWALKDYGFAVVLSSRFADIFRGNAGKAGLLAAQMEQTD
CCCEEEEECCCCCCCCCCCCEEEEECCCCEEEEEHHHHHHHHCCCCCCCCEEEEHHHHHH
IELLWKQLEQTPGAQVTVSLEERTVTCEGNVYPFFVDDYTRWRLMEGLDDVGLTLRKEAE
HHHHHHHHHHCCCCEEEEEEECEEEEECCCEEEEEECCCHHHHHHHCCCCCCEEEECCCC
IAAFEARRPSFKPVTQ
EEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910