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The map label for this gene is leuB

Identifier: 38233699

GI number: 38233699

Start: 1092016

End: 1093035

Strand: Direct

Name: leuB

Synonym: DIP1105

Alternate gene names: 38233699

Gene position: 1092016-1093035 (Clockwise)

Preceding gene: 38233698

Following gene: 38233700

Centisome position: 43.88

GC content: 52.06

Gene sequence:

>1020_bases
ATGAAACTAGCTGTTATTGGTGGCGATGGCATTGGCCCAGAGGTTACTGCAGAAGCTCTGAAGGTGCTCAATGCGGTACG
CGATGACATTGAAGTCACCGATTATGACCTCGGCGCACGCCGTTACCTGCGCAACGGTGAACTTCTCGCAGATGCTGATT
TGGTATCTTTACGCGAACATGATGCTATTTTGCTGGGGGCAATCGGGGCACCAGGCGAGGTTCCACCAGGAGTGTTGGAG
CGTGGATTGCTGTTGAAAATGCGCTTCGCATTGGATCATCATGTGAATCTGCGACCTTCTAAGCTGTATCCCACAGCGAC
GTCGCCGTTGGCTAATCCTGGCGATATCGATTTTGTGGTGGTCCGTGAGGGTACTGAGGGTCTTTATTGTGGCAATGGCG
GTACTTTGCGTGAGGGGACGCCGCATGAAATAGCGTCGGAGGTTTCCCAAAACACTCGCTACGGTGTGGAACGAGTTGTG
CGCGATGCTTTTGAGCGCGCTCAAAACCGCAAAAAGCACCTTACGCTGGTGCATAAAACCAATGTTCTTGTTAATGCTGG
TGGTTTGTGGCAGCGTACCGTCAATGAGATTGCCACTGAGTATCCAGAGGTTACAGTCGATTACAACCACATTGATGCTG
CAACTATTTATATGGTCACGGACCCCAGTCGCTATGACGTTATTGTGACCGATAATTTGTTTGGAGACATCCTCACCGAC
CTTGCTGGTGCGGTAACGGGTGGTATCGGTTTAGCGGCTTCGGGCAACATTGATGCCACTGGTGTGAATCCTTCAATGTT
TGAGCCTGTTCATGGTTCGGCCCCAGACATTGCGGGTCAGGGGATTGCTGATCCTACTGCTGCTATTTTGTCGGCGGCGA
TGTTACTGCGTCATGTGGGCGATGAGTCTAATGCCCAGCGCATTGAAGCGGCGGTAACCTATGATGTTGCAGAGCGGCCT
GCTGGACCGGTTAAGACTGTCGAGGTGGGTGATCGGATCGTTGCTGCATTGCAACGGTAA

Upstream 100 bases:

>100_bases
CTGGGGGAAAGGTCCGTTTGTGCTTTGGCGTCAGTTTTTGGGGATCGGTCTGCTACACTCGGGTACAAAGTTCACATAGT
GGGAAGTGAGGTTCCATAAT

Downstream 100 bases:

>100_bases
GGCGTACTCGAGGAGATCTCGGGGTTCCTGAGTTGTCAGGAACCCTTTAGTAAACTCGATCCGCAACACATCCGCCATGT
CGTGTCTACTGCTACCATGC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIEVTDYDLGARRYLRNGELLADADLVSLREHDAILLGAIGAPGEVPPGVLE
RGLLLKMRFALDHHVNLRPSKLYPTATSPLANPGDIDFVVVREGTEGLYCGNGGTLREGTPHEIASEVSQNTRYGVERVV
RDAFERAQNRKKHLTLVHKTNVLVNAGGLWQRTVNEIATEYPEVTVDYNHIDAATIYMVTDPSRYDVIVTDNLFGDILTD
LAGAVTGGIGLAASGNIDATGVNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHVGDESNAQRIEAAVTYDVAERP
AGPVKTVEVGDRIVAALQR

Sequences:

>Translated_339_residues
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIEVTDYDLGARRYLRNGELLADADLVSLREHDAILLGAIGAPGEVPPGVLE
RGLLLKMRFALDHHVNLRPSKLYPTATSPLANPGDIDFVVVREGTEGLYCGNGGTLREGTPHEIASEVSQNTRYGVERVV
RDAFERAQNRKKHLTLVHKTNVLVNAGGLWQRTVNEIATEYPEVTVDYNHIDAATIYMVTDPSRYDVIVTDNLFGDILTD
LAGAVTGGIGLAASGNIDATGVNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHVGDESNAQRIEAAVTYDVAERP
AGPVKTVEVGDRIVAALQR
>Mature_339_residues
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIEVTDYDLGARRYLRNGELLADADLVSLREHDAILLGAIGAPGEVPPGVLE
RGLLLKMRFALDHHVNLRPSKLYPTATSPLANPGDIDFVVVREGTEGLYCGNGGTLREGTPHEIASEVSQNTRYGVERVV
RDAFERAQNRKKHLTLVHKTNVLVNAGGLWQRTVNEIATEYPEVTVDYNHIDAATIYMVTDPSRYDVIVTDNLFGDILTD
LAGAVTGGIGLAASGNIDATGVNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHVGDESNAQRIEAAVTYDVAERP
AGPVKTVEVGDRIVAALQR

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=315, Percent_Identity=35.5555555555556, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI4758582, Length=312, Percent_Identity=30.1282051282051, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI28178838, Length=312, Percent_Identity=30.1282051282051, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI28178816, Length=313, Percent_Identity=28.1150159744409, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI28178821, Length=313, Percent_Identity=28.1150159744409, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI28178819, Length=160, Percent_Identity=32.5, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788101, Length=358, Percent_Identity=43.0167597765363, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI87081683, Length=355, Percent_Identity=41.6901408450704, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1787381, Length=390, Percent_Identity=29.4871794871795, Blast_Score=122, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71986051, Length=334, Percent_Identity=36.2275449101796, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17550882, Length=332, Percent_Identity=29.5180722891566, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=309, Percent_Identity=30.42071197411, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=344, Percent_Identity=28.7790697674419, Blast_Score=121, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6324709, Length=323, Percent_Identity=36.2229102167183, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319830, Length=366, Percent_Identity=33.879781420765, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6322097, Length=347, Percent_Identity=31.1239193083574, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6324291, Length=321, Percent_Identity=32.0872274143302, Blast_Score=125, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24643270, Length=350, Percent_Identity=35.4285714285714, Blast_Score=187, Evalue=8e-48,
Organism=Drosophila melanogaster, GI24643268, Length=350, Percent_Identity=35.4285714285714, Blast_Score=187, Evalue=8e-48,
Organism=Drosophila melanogaster, GI24661184, Length=317, Percent_Identity=33.1230283911672, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI161078637, Length=314, Percent_Identity=29.6178343949045, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI161078635, Length=314, Percent_Identity=29.6178343949045, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI161078633, Length=314, Percent_Identity=29.6178343949045, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24650122, Length=314, Percent_Identity=29.6178343949045, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI281362242, Length=329, Percent_Identity=29.1793313069909, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24648872, Length=329, Percent_Identity=29.1793313069909, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI161078639, Length=309, Percent_Identity=30.0970873786408, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI20130355, Length=315, Percent_Identity=25.7142857142857, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEU3_CORDI (Q6NHM7)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939466.1
- ProteinModelPortal:   Q6NHM7
- SMR:   Q6NHM7
- GeneID:   2650847
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1105
- NMPDR:   fig|257309.1.peg.1057
- HOGENOM:   HBG518924
- OMA:   AATIFMV
- ProtClustDB:   PRK03437
- BioCyc:   CDIP257309:DIP1105-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01035
- InterPro:   IPR019818
- InterPro:   IPR001804
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 36124; Mature: 36124

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 88-88 BINDING 98-98 BINDING 122-122 BINDING 212-212

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIEVTDYDLGARRYLRNGELLADADLVSLREH
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHCCCCEEECCHHHHHHCC
DAILLGAIGAPGEVPPGVLERGLLLKMRFALDHHVNLRPSKLYPTATSPLANPGDIDFVV
CEEEEEECCCCCCCCHHHHHCCHHEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEE
VREGTEGLYCGNGGTLREGTPHEIASEVSQNTRYGVERVVRDAFERAQNRKKHLTLVHKT
EECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEEC
NVLVNAGGLWQRTVNEIATEYPEVTVDYNHIDAATIYMVTDPSRYDVIVTDNLFGDILTD
CEEEECCCHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCEEEEEECCHHHHHHHH
LAGAVTGGIGLAASGNIDATGVNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHVG
HHHHHHCCCCEEECCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
DESNAQRIEAAVTYDVAERPAGPVKTVEVGDRIVAALQR
CCCCHHHHHHEEEEEHHHCCCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIEVTDYDLGARRYLRNGELLADADLVSLREH
CEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHCCCCEEECCHHHHHHCC
DAILLGAIGAPGEVPPGVLERGLLLKMRFALDHHVNLRPSKLYPTATSPLANPGDIDFVV
CEEEEEECCCCCCCCHHHHHCCHHEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEE
VREGTEGLYCGNGGTLREGTPHEIASEVSQNTRYGVERVVRDAFERAQNRKKHLTLVHKT
EECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEEC
NVLVNAGGLWQRTVNEIATEYPEVTVDYNHIDAATIYMVTDPSRYDVIVTDNLFGDILTD
CEEEECCCHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCEEEEEECCHHHHHHHH
LAGAVTGGIGLAASGNIDATGVNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHVG
HHHHHHCCCCEEECCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
DESNAQRIEAAVTYDVAERPAGPVKTVEVGDRIVAALQR
CCCCHHHHHHEEEEEHHHCCCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910