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The map label for this gene is yfiY [H]

Identifier: 38233656

GI number: 38233656

Start: 1040784

End: 1041827

Strand: Direct

Name: yfiY [H]

Synonym: DIP1062

Alternate gene names: 38233656

Gene position: 1040784-1041827 (Clockwise)

Preceding gene: 38233652

Following gene: 38233658

Centisome position: 41.82

GC content: 49.14

Gene sequence:

>1044_bases
ATGTTCGAAATATCCCGATCTCGTGCGAAAGCAATGCGTCCTTTCCTTGCCCTCGCCACAGCCACTGTAATGATGCTGTC
CCTCGCAGCGTGTTCTTCTTCTGACAATTCCTCGACTAACGCTCAAGGTAACACTGATGTTGCCGTGGGTGGCCAGCGTT
TTAGCACCGCTGATCAAGAAACTGCCAAGCTTGGTAGTGATGCACAACCTGGTCAATTCCCACGTACCGTCGTTCATGCT
GCTGGAACAACGGAGATCACAACAAAGCCCGAGCGTGTTGTTGTCCTTGATACCGGTGAATTAGATAGTGTCTTGTCTCT
TGGGATCACCCCAGTAGGAATGACCACTACCAAGGGTGCTAATCCAGTGCCGAGCTATCTCGCCGATAAGGTAAAAGACG
TGGAGCGAGTTGGCACTATCAACGAGCTTAATATCGAGGCAATTGCGGCTTTGAAGCCCGACCTGATTATTGGTTCGCAA
TTGCGTGCCGACAAACTGTATCCGCAGCTTTCTGACATTGCACCTACAGTTTTTTCCATCCGACCAGGTTCTCCATGGAA
GGAAAACTTCCTCCTCGTTGGTGAGGCACTTGGTATGGAAAAAGAAGCGGAAGAAAAACTGAATGAGTATGCTGACCGCG
TTGCTGAACTAAAGAACAACGTTCCTCAAAATACTGAGGTTTCTCTCGTTAGGTTTATGCCTAACAAGCTGAGGCTGTAT
GGAAACAAGTCGTTGATCGGTGTTGTTCTTGCCGATGCTGGGCTTGCTCGCCCAGAAAAACAAAACGTTGATGATCTTGC
TGTTGAAATTTCTCCAGAAAACATCGATCAAGCTGCAGGATCGGTCATCTTCTATACTTCCTACGGAAAGCCAGATGCTA
CAGGGGAAACCGCTGTAGTTGAGGGGCCGGCATGGAAAAACCTTGAAGCAGTGGCTGCGGGTAAGGCCCACCGCGTTAAT
GACGATGTGTGGTTCCTTGGTCTAGGGCCAACAGGCGCTATGGAGATTGTCTCGGACCTTCACGACTACTTACTGAAGAA
ATAA

Upstream 100 bases:

>100_bases
AGGAAAGTGATAACTAAAGGCAATGGTAGCCGGACACGAGGTTGTCGAAGCATTAGATAATTCTAACCAATTAATTTGAA
ATTAGGGTAACCTGTCCAAC

Downstream 100 bases:

>100_bases
AATCCTTCCGAAAAATCCTGATCCGCTTCTAGTGAGCTAACAATCACTGCGATGCAAGTTCACTAGGAGCGGATCATTTT
CTTTGCCCTATTGAGGCTAA

Product: putative iron-siderophore uptake system exported solute-binding component

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MFEISRSRAKAMRPFLALATATVMMLSLAACSSSDNSSTNAQGNTDVAVGGQRFSTADQETAKLGSDAQPGQFPRTVVHA
AGTTEITTKPERVVVLDTGELDSVLSLGITPVGMTTTKGANPVPSYLADKVKDVERVGTINELNIEAIAALKPDLIIGSQ
LRADKLYPQLSDIAPTVFSIRPGSPWKENFLLVGEALGMEKEAEEKLNEYADRVAELKNNVPQNTEVSLVRFMPNKLRLY
GNKSLIGVVLADAGLARPEKQNVDDLAVEISPENIDQAAGSVIFYTSYGKPDATGETAVVEGPAWKNLEAVAAGKAHRVN
DDVWFLGLGPTGAMEIVSDLHDYLLKK

Sequences:

>Translated_347_residues
MFEISRSRAKAMRPFLALATATVMMLSLAACSSSDNSSTNAQGNTDVAVGGQRFSTADQETAKLGSDAQPGQFPRTVVHA
AGTTEITTKPERVVVLDTGELDSVLSLGITPVGMTTTKGANPVPSYLADKVKDVERVGTINELNIEAIAALKPDLIIGSQ
LRADKLYPQLSDIAPTVFSIRPGSPWKENFLLVGEALGMEKEAEEKLNEYADRVAELKNNVPQNTEVSLVRFMPNKLRLY
GNKSLIGVVLADAGLARPEKQNVDDLAVEISPENIDQAAGSVIFYTSYGKPDATGETAVVEGPAWKNLEAVAAGKAHRVN
DDVWFLGLGPTGAMEIVSDLHDYLLKK
>Mature_347_residues
MFEISRSRAKAMRPFLALATATVMMLSLAACSSSDNSSTNAQGNTDVAVGGQRFSTADQETAKLGSDAQPGQFPRTVVHA
AGTTEITTKPERVVVLDTGELDSVLSLGITPVGMTTTKGANPVPSYLADKVKDVERVGTINELNIEAIAALKPDLIIGSQ
LRADKLYPQLSDIAPTVFSIRPGSPWKENFLLVGEALGMEKEAEEKLNEYADRVAELKNNVPQNTEVSLVRFMPNKLRLY
GNKSLIGVVLADAGLARPEKQNVDDLAVEISPENIDQAAGSVIFYTSYGKPDATGETAVVEGPAWKNLEAVAAGKAHRVN
DDVWFLGLGPTGAMEIVSDLHDYLLKK

Specific function: Part of the ABC transporter complex yfiYZ/yfhA/yusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of yfiZ/yfhA (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable). Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082411, Length=270, Percent_Identity=25.5555555555556, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 37142; Mature: 37142

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEISRSRAKAMRPFLALATATVMMLSLAACSSSDNSSTNAQGNTDVAVGGQRFSTADQE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCHH
TAKLGSDAQPGQFPRTVVHAAGTTEITTKPERVVVLDTGELDSVLSLGITPVGMTTTKGA
HHHCCCCCCCCCCCHHHEECCCCCEEECCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCC
NPVPSYLADKVKDVERVGTINELNIEAIAALKPDLIIGSQLRADKLYPQLSDIAPTVFSI
CCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHCCCHHHCCCEEEEE
RPGSPWKENFLLVGEALGMEKEAEEKLNEYADRVAELKNNVPQNTEVSLVRFMPNKLRLY
CCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEE
GNKSLIGVVLADAGLARPEKQNVDDLAVEISPENIDQAAGSVIFYTSYGKPDATGETAVV
CCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHCCCEEEEEECCCCCCCCCEEEE
EGPAWKNLEAVAAGKAHRVNDDVWFLGLGPTGAMEIVSDLHDYLLKK
ECCCCCCCHHHHCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFEISRSRAKAMRPFLALATATVMMLSLAACSSSDNSSTNAQGNTDVAVGGQRFSTADQE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCHH
TAKLGSDAQPGQFPRTVVHAAGTTEITTKPERVVVLDTGELDSVLSLGITPVGMTTTKGA
HHHCCCCCCCCCCCHHHEECCCCCEEECCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCC
NPVPSYLADKVKDVERVGTINELNIEAIAALKPDLIIGSQLRADKLYPQLSDIAPTVFSI
CCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHCCCHHHCCCEEEEE
RPGSPWKENFLLVGEALGMEKEAEEKLNEYADRVAELKNNVPQNTEVSLVRFMPNKLRLY
CCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEE
GNKSLIGVVLADAGLARPEKQNVDDLAVEISPENIDQAAGSVIFYTSYGKPDATGETAVV
CCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHCCCEEEEEECCCCCCCCCEEEE
EGPAWKNLEAVAAGKAHRVNDDVWFLGLGPTGAMEIVSDLHDYLLKK
ECCCCCCCHHHHCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]