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The map label for this gene is yiaE [C]

Identifier: 38233503

GI number: 38233503

Start: 878149

End: 879078

Strand: Direct

Name: yiaE [C]

Synonym: DIP0907

Alternate gene names: 38233503

Gene position: 878149-879078 (Clockwise)

Preceding gene: 38233502

Following gene: 38233504

Centisome position: 35.29

GC content: 54.73

Gene sequence:

>930_bases
GTGAAGTACTTCATGGGTCCACATGTATGGCAGCGCACGATTGCTGATCTTGAAGCGCTGGGGCATCAGCGCGTCGATAC
GTTTGAGCAGGCTGAGGCTTACATCAATACGGAGCCGCGTCCGCTGCGGATCCCTACAATCCCCGAGTCGGTCGGTTTTG
TGCAGCATTGTTTTACAGGCGTTAATCAGCTTATCGACGCCCATGTGATCACCGAAACTGGGGTTCGCTGGGCGAATATG
GCCGGTGGGTTTGCACAACCGGTCGCTGAATCCGCCTTGGGGCTTATGCTTTCGCAGGCGCATCATCATAAGGCTTTTGC
GCTTGCAGGAACGTGGCGAGTAGCGCGCGAGCTTGACCGAATCCAACAGTGGTTACACTCGGTAGGTGAGCCGCGTTGCG
TGGTGATCTTTGGTGCGGGCGGGATTGCTAAGGAGCTGATCCGTCTCTTAGAACCTTTTGGCATGCATGTGATTGCGGTG
AACCGTTCTGGTCGCAGCGTGGCGGGTGCTGATGAAACCTTCGCTATGAAAGATGCGCAGGGGCTCTGGTCGCGCGGGGA
TTTCATCGTCAACATTTTGCCGCTTACCCAAGAAACCAAAGGGCTAGTTGATCGCGATGTGTTTGCACAAATGAAGCCCT
CGGCGATCTTTATTAACGTAGGCCGTGGCGCCACTGTTGTAACCGACGATCTTGTCGATGCCCTCCAACGAGGCGTTATC
GCTGGTGCTGGCTTAGAAGTGGTCGACCCCGAGCCGTTACCAGATTCTCACGCGTTACACAGCATGCCGAACTGTACGAT
CACTCCGCATATGGCAGCTTCTGATCATGTAGCCGAACTTCATGTGGCGCGGATTTTTGATGCGAATGCCCAAGCTTTTA
CCCGTGGGGAAACGATGCCAACGGAGGTGAATCCGCACTTAGGTTATTAG

Upstream 100 bases:

>100_bases
GGTGGGGACGTTGTGCCTTGTGGGGACGGTGACGTTGTGCTTGGTGGCGTCGGCACGCGCGGTGCTGCGTGGGTAGCTGA
TCTCCGTATGATGGGGGCTT

Downstream 100 bases:

>100_bases
GCGCAGGTGTGTACGGTTAACTCTTATGCAATGGCGTCTTTTTACTCGGCCGAATCAGGTTCCTCCACGCATGGTGACGT
GGTCGTGGCTTGCGCCGGCC

Product: putative reductase

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKYFMGPHVWQRTIADLEALGHQRVDTFEQAEAYINTEPRPLRIPTIPESVGFVQHCFTGVNQLIDAHVITETGVRWANM
AGGFAQPVAESALGLMLSQAHHHKAFALAGTWRVARELDRIQQWLHSVGEPRCVVIFGAGGIAKELIRLLEPFGMHVIAV
NRSGRSVAGADETFAMKDAQGLWSRGDFIVNILPLTQETKGLVDRDVFAQMKPSAIFINVGRGATVVTDDLVDALQRGVI
AGAGLEVVDPEPLPDSHALHSMPNCTITPHMAASDHVAELHVARIFDANAQAFTRGETMPTEVNPHLGY

Sequences:

>Translated_309_residues
MKYFMGPHVWQRTIADLEALGHQRVDTFEQAEAYINTEPRPLRIPTIPESVGFVQHCFTGVNQLIDAHVITETGVRWANM
AGGFAQPVAESALGLMLSQAHHHKAFALAGTWRVARELDRIQQWLHSVGEPRCVVIFGAGGIAKELIRLLEPFGMHVIAV
NRSGRSVAGADETFAMKDAQGLWSRGDFIVNILPLTQETKGLVDRDVFAQMKPSAIFINVGRGATVVTDDLVDALQRGVI
AGAGLEVVDPEPLPDSHALHSMPNCTITPHMAASDHVAELHVARIFDANAQAFTRGETMPTEVNPHLGY
>Mature_309_residues
MKYFMGPHVWQRTIADLEALGHQRVDTFEQAEAYINTEPRPLRIPTIPESVGFVQHCFTGVNQLIDAHVITETGVRWANM
AGGFAQPVAESALGLMLSQAHHHKAFALAGTWRVARELDRIQQWLHSVGEPRCVVIFGAGGIAKELIRLLEPFGMHVIAV
NRSGRSVAGADETFAMKDAQGLWSRGDFIVNILPLTQETKGLVDRDVFAQMKPSAIFINVGRGATVVTDDLVDALQRGVI
AGAGLEVVDPEPLPDSHALHSMPNCTITPHMAASDHVAELHVARIFDANAQAFTRGETMPTEVNPHLGY

Specific function: Unknown

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=240, Percent_Identity=33.75, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI23308577, Length=261, Percent_Identity=26.0536398467433, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI87082289, Length=237, Percent_Identity=32.0675105485232, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081824, Length=174, Percent_Identity=32.183908045977, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787645, Length=286, Percent_Identity=23.7762237762238, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=250, Percent_Identity=28, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25147481, Length=224, Percent_Identity=26.7857142857143, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322116, Length=247, Percent_Identity=28.3400809716599, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320925, Length=323, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574286, Length=251, Percent_Identity=28.6852589641434, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585516, Length=231, Percent_Identity=33.7662337662338, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI45552429, Length=168, Percent_Identity=31.547619047619, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28574284, Length=169, Percent_Identity=31.3609467455621, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585514, Length=169, Percent_Identity=31.3609467455621, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28574282, Length=169, Percent_Identity=31.3609467455621, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45551003, Length=169, Percent_Identity=31.3609467455621, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28571528, Length=171, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19921140, Length=158, Percent_Identity=28.4810126582279, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 33744; Mature: 33744

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYFMGPHVWQRTIADLEALGHQRVDTFEQAEAYINTEPRPLRIPTIPESVGFVQHCFTG
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHH
VNQLIDAHVITETGVRWANMAGGFAQPVAESALGLMLSQAHHHKAFALAGTWRVARELDR
HHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
IQQWLHSVGEPRCVVIFGAGGIAKELIRLLEPFGMHVIAVNRSGRSVAGADETFAMKDAQ
HHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCEECCCCCHHHHHHHH
GLWSRGDFIVNILPLTQETKGLVDRDVFAQMKPSAIFINVGRGATVVTDDLVDALQRGVI
CCCCCCCEEEEEEECCCHHHCHHHHHHHHHCCCCEEEEECCCCCEEEHHHHHHHHHHCEE
AGAGLEVVDPEPLPDSHALHSMPNCTITPHMAASDHVAELHVARIFDANAQAFTRGETMP
ECCCEEEECCCCCCCCHHHHCCCCCEECCCCCCCCHHHHHHHHHHCCCCCHHEECCCCCC
TEVNPHLGY
CCCCCCCCC
>Mature Secondary Structure
MKYFMGPHVWQRTIADLEALGHQRVDTFEQAEAYINTEPRPLRIPTIPESVGFVQHCFTG
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHH
VNQLIDAHVITETGVRWANMAGGFAQPVAESALGLMLSQAHHHKAFALAGTWRVARELDR
HHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
IQQWLHSVGEPRCVVIFGAGGIAKELIRLLEPFGMHVIAVNRSGRSVAGADETFAMKDAQ
HHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCEECCCCCHHHHHHHH
GLWSRGDFIVNILPLTQETKGLVDRDVFAQMKPSAIFINVGRGATVVTDDLVDALQRGVI
CCCCCCCEEEEEEECCCHHHCHHHHHHHHHCCCCEEEEECCCCCEEEHHHHHHHHHHCEE
AGAGLEVVDPEPLPDSHALHSMPNCTITPHMAASDHVAELHVARIFDANAQAFTRGETMP
ECCCEEEECCCCCCCCHHHHCCCCCEECCCCCCCCHHHHHHHHHHCCCCCHHEECCCCCC
TEVNPHLGY
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]