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The map label for this gene is purN [H]

Identifier: 38233435

GI number: 38233435

Start: 816768

End: 817391

Strand: Direct

Name: purN [H]

Synonym: DIP0838

Alternate gene names: 38233435

Gene position: 816768-817391 (Clockwise)

Preceding gene: 38233434

Following gene: 38233436

Centisome position: 32.82

GC content: 53.85

Gene sequence:

>624_bases
ATGGTGGCTGTGACGAGTTTATCCAGTACGGAGCAAGCAGCACCGATCGTGGTGCTTGCTTCAGGTTCCGGAACCCTCTT
GCAGGCCATCATCGACAATCAGGAACGATATCGCGTGGTCGCCGTCGTGGCAGACGTCGAGTGTTTTGCACTCGAACGAG
CCAAACAAGCAGGGATTCCAACGCACATTGTGCCCTTAGAAAAAGGCGCAGATCGTCATGAATGGAACCTCGCCCTCGCA
CGCACCGTAGAACGCTATGAGCCAACAATCGTAGTCTCCGCCGGATTTATGAAGATCCTCGGCGAAGGATTCCTGCGAAC
GTTCGAAGGTCGTACCATCAATACGCACCCTGCGTTGCTCCCTGCATTCCCAGGAGCACATGCCGTGCGAGATGCATTGA
ACTATGGCGTCAGAGTCACCGGTTCCACTGTTCACTTTGTAGACAGTGGCGTTGACACTGGCGCCATTATTGCGCAACGG
CCCGTTTCCATCGAACGGGGAGAAGACGAGTCACATCTTCATGAAAGAATCAAACAAGTTGAGCGCCAGCTCATCGTGTC
CGTGTTGAACTCCGCCGTGACGGAGAAAGAATCAGGAGAGGTTTCCTTCACTCATGAGCGATGA

Upstream 100 bases:

>100_bases
GAAAATGAAGGGGAAGAAGAGACAACGGAAAGCGATCAGGAGTCTGATTCGAAGCCAAAGGATGACACGGAATAGGGCAT
AACGGGCTTGAACCGTTACG

Downstream 100 bases:

>100_bases
CCGCAAGCAGATCAAGCGCGCATTAATTAGTGTGTACGACAAGACCGGACTGGAAGATCTGGCCCGTGCCCTCAATGATG
CGGGTGTGGAGATCGTTTCC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MVAVTSLSSTEQAAPIVVLASGSGTLLQAIIDNQERYRVVAVVADVECFALERAKQAGIPTHIVPLEKGADRHEWNLALA
RTVERYEPTIVVSAGFMKILGEGFLRTFEGRTINTHPALLPAFPGAHAVRDALNYGVRVTGSTVHFVDSGVDTGAIIAQR
PVSIERGEDESHLHERIKQVERQLIVSVLNSAVTEKESGEVSFTHER

Sequences:

>Translated_207_residues
MVAVTSLSSTEQAAPIVVLASGSGTLLQAIIDNQERYRVVAVVADVECFALERAKQAGIPTHIVPLEKGADRHEWNLALA
RTVERYEPTIVVSAGFMKILGEGFLRTFEGRTINTHPALLPAFPGAHAVRDALNYGVRVTGSTVHFVDSGVDTGAIIAQR
PVSIERGEDESHLHERIKQVERQLIVSVLNSAVTEKESGEVSFTHER
>Mature_207_residues
MVAVTSLSSTEQAAPIVVLASGSGTLLQAIIDNQERYRVVAVVADVECFALERAKQAGIPTHIVPLEKGADRHEWNLALA
RTVERYEPTIVVSAGFMKILGEGFLRTFEGRTINTHPALLPAFPGAHAVRDALNYGVRVTGSTVHFVDSGVDTGAIIAQR
PVSIERGEDESHLHERIKQVERQLIVSVLNSAVTEKESGEVSFTHER

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=209, Percent_Identity=35.8851674641148, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI209869995, Length=209, Percent_Identity=35.8851674641148, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI209869993, Length=209, Percent_Identity=35.8851674641148, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1788846, Length=180, Percent_Identity=39.4444444444444, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787483, Length=138, Percent_Identity=29.7101449275362, Blast_Score=77, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=40.2234636871508, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320616, Length=190, Percent_Identity=32.6315789473684, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24582400, Length=174, Percent_Identity=40.8045977011494, Blast_Score=127, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22488; Mature: 22488

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVTSLSSTEQAAPIVVLASGSGTLLQAIIDNQERYRVVAVVADVECFALERAKQAGIP
CEEEECCCCCCCCCCEEEEECCCCHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHCCCC
THIVPLEKGADRHEWNLALARTVERYEPTIVVSAGFMKILGEGFLRTFEGRTINTHPALL
EEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHCCCEECCCCCEE
PAFPGAHAVRDALNYGVRVTGSTVHFVDSGVDTGAIIAQRPVSIERGEDESHLHERIKQV
CCCCCHHHHHHHHHCCEEEECCEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
ERQLIVSVLNSAVTEKESGEVSFTHER
HHHHHHHHHHHHHCCCCCCCEEEEECC
>Mature Secondary Structure
MVAVTSLSSTEQAAPIVVLASGSGTLLQAIIDNQERYRVVAVVADVECFALERAKQAGIP
CEEEECCCCCCCCCCEEEEECCCCHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHCCCC
THIVPLEKGADRHEWNLALARTVERYEPTIVVSAGFMKILGEGFLRTFEGRTINTHPALL
EEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHCCCEECCCCCEE
PAFPGAHAVRDALNYGVRVTGSTVHFVDSGVDTGAIIAQRPVSIERGEDESHLHERIKQV
CCCCCHHHHHHHHHCCEEEECCEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
ERQLIVSVLNSAVTEKESGEVSFTHER
HHHHHHHHHHHHHCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]