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The map label for this gene is folA [H]

Identifier: 38233421

GI number: 38233421

Start: 797137

End: 797640

Strand: Reverse

Name: folA [H]

Synonym: DIP0824

Alternate gene names: 38233421

Gene position: 797640-797137 (Counterclockwise)

Preceding gene: 38233422

Following gene: 38233420

Centisome position: 32.05

GC content: 60.32

Gene sequence:

>504_bases
ATGCTGCGCGCGATTTGGGCTCAGTCCACTGATGGGGTGATTGGCGATGGCAACGACATGCCCTGGCATGTTCCCGAAGA
CCTCGCCTATTTCAAGGCCACCACTTTGGGCGAACCTGTGATTATGGGTCGCACCACGTGGACTACGATCCCAGAACGCT
TCCGCCCGCTGCCAGGGCGTCGTAACTTTGTGCTCTCCTCCCGAGCACCCGGCGCGTGGAGCACGGGTGCCGAGGTCGTC
ACTGCAATCCCCGAGCTCGACTGCGATGCATGGATCATGGGCGGCGGCGCCGTCTACACAAGCACACTTCCGCTGGTAGA
CGAGGTAGTGATCACGCTTATCGACGTCACCCTCGCTCCCGTACTCGGCGACGCAGCAGTATATGCACCAGCCCTCGACG
ATAGCTTTGAGGTTGTTGAGGAATCCCCATGGCAGCAATCTGCTTCCGGGACCGTGCTGGGTACTCCGGCCCGCTATAAG
TTCCAAAGGTTGCGCAGGCGTTAA

Upstream 100 bases:

>100_bases
CATTGCGGAAAGCCTCCTCGATCTTCGACTACGACTTCGACGATTTCACTATCGAGGGCTACGATCCTCACCCAGCGATT
CGTGGCGAGGTAGCAGTGTA

Downstream 100 bases:

>100_bases
AAGCAAAGGAAGATGGTCACATGTCTGATAACAACCATGTGACCATCTACGCTGCCGACTGGTGCCCATTTTGCCAGCGC
CTGATTAAGGCGCTCAACCG

Product: putative folate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MLRAIWAQSTDGVIGDGNDMPWHVPEDLAYFKATTLGEPVIMGRTTWTTIPERFRPLPGRRNFVLSSRAPGAWSTGAEVV
TAIPELDCDAWIMGGGAVYTSTLPLVDEVVITLIDVTLAPVLGDAAVYAPALDDSFEVVEESPWQQSASGTVLGTPARYK
FQRLRRR

Sequences:

>Translated_167_residues
MLRAIWAQSTDGVIGDGNDMPWHVPEDLAYFKATTLGEPVIMGRTTWTTIPERFRPLPGRRNFVLSSRAPGAWSTGAEVV
TAIPELDCDAWIMGGGAVYTSTLPLVDEVVITLIDVTLAPVLGDAAVYAPALDDSFEVVEESPWQQSASGTVLGTPARYK
FQRLRRR
>Mature_167_residues
MLRAIWAQSTDGVIGDGNDMPWHVPEDLAYFKATTLGEPVIMGRTTWTTIPERFRPLPGRRNFVLSSRAPGAWSTGAEVV
TAIPELDCDAWIMGGGAVYTSTLPLVDEVVITLIDVTLAPVLGDAAVYAPALDDSFEVVEESPWQQSASGTVLGTPARYK
FQRLRRR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=179, Percent_Identity=38.5474860335196, Blast_Score=90, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324810, Length=157, Percent_Identity=29.2993630573248, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18234; Mature: 18234

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRAIWAQSTDGVIGDGNDMPWHVPEDLAYFKATTLGEPVIMGRTTWTTIPERFRPLPGR
CCCEEECCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCCCC
RNFVLSSRAPGAWSTGAEVVTAIPELDCDAWIMGGGAVYTSTLPLVDEVVITLIDVTLAP
CCEEEECCCCCCCCCCCHHHHHCCCCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHHH
VLGDAAVYAPALDDSFEVVEESPWQQSASGTVLGTPARYKFQRLRRR
HHCCCCEECCCCCCCHHHHHCCCCCCCCCCCEEECCHHHHHHHHHCC
>Mature Secondary Structure
MLRAIWAQSTDGVIGDGNDMPWHVPEDLAYFKATTLGEPVIMGRTTWTTIPERFRPLPGR
CCCEEECCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCCCC
RNFVLSSRAPGAWSTGAEVVTAIPELDCDAWIMGGGAVYTSTLPLVDEVVITLIDVTLAP
CCEEEECCCCCCCCCCCHHHHHCCCCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHHH
VLGDAAVYAPALDDSFEVVEESPWQQSASGTVLGTPARYKFQRLRRR
HHCCCCEECCCCCCCHHHHHCCCCCCCCCCCEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]