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The map label for this gene is thyA

Identifier: 38233422

GI number: 38233422

Start: 797640

End: 798452

Strand: Reverse

Name: thyA

Synonym: DIP0825

Alternate gene names: 38233422

Gene position: 798452-797640 (Counterclockwise)

Preceding gene: 38233423

Following gene: 38233421

Centisome position: 32.08

GC content: 53.26

Gene sequence:

>813_bases
ATGAGCACCAACAACACCATAAAAACCCCGTATGAAGACCTTCTTCGCACAATCCTTGAACAGGGCTCCCACAAAGACGA
CCGTACCGGCACCGGTACAACCAGCCTTTTTGGCCAACAGATGCGTTTCGATCTCACCGAGGCCTTCCCACTCATCACCA
CCAAGAAGGTGTATTGGAAAGGTGTAATCGGAGAACTCCTCTGGTTCCTGCAAGGCTCCTCCAATGTTCGCTGGCTCCAA
AAGCGCAACATTCACATCTGGGATGAATGGGCTTCCGAAGAAGGCGAACTGGGACCTGTTTATGGTGTTCAATGGCGCAG
CTGGCCTACCCCTGATGGGCAGCATGTGGACCAGATCGCCCAAGCACTAGATATACTCAAAAACAATCCGGACTCTAGGC
GCAACATCGTCTCAGCGTGGAACGTGGCCGATCTCAACAATATGGCGCTTCCACCGTGCCACTTGTTGTTCCAGCTTTAT
GTTGCCGATGGAAAGTTGAGCTGCCAGCTCTACCAGCGCAGCGCAGACATGTTCTTAGGTGTTCCGTTTAATATCGCCTC
CTACTCCGCTCTCACACATATGCTCGCACAACAAGCAGGCCTTAAGGTCGGCGAGTTCATTTGGACTGGCGGCGACTGCC
ACATCTACGACAATCATCGCGAGCAGGTTCTCACCCAGCTCTCACGTGAACCTCGCCCCTACCCTCAGTTGACATTGCGG
AAAGCCTCCTCGATCTTCGACTACGACTTCGACGATTTCACTATCGAGGGCTACGATCCTCACCCAGCGATTCGTGGCGA
GGTAGCAGTGTAA

Upstream 100 bases:

>100_bases
CTACTAATCGTATAGTTGTCACTCGGCGGGAAACCTCTCACATTGAGCACGGAGGTTTCCCACCGGAATTTCTTTGCCCG
CTATCGACTTGATTGTGAAT

Downstream 100 bases:

>100_bases
TGCTGCGCGCGATTTGGGCTCAGTCCACTGATGGGGTGATTGGCGATGGCAACGACATGCCCTGGCATGTTCCCGAAGAC
CTCGCCTATTTCAAGGCCAC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSTNNTIKTPYEDLLRTILEQGSHKDDRTGTGTTSLFGQQMRFDLTEAFPLITTKKVYWKGVIGELLWFLQGSSNVRWLQ
KRNIHIWDEWASEEGELGPVYGVQWRSWPTPDGQHVDQIAQALDILKNNPDSRRNIVSAWNVADLNNMALPPCHLLFQLY
VADGKLSCQLYQRSADMFLGVPFNIASYSALTHMLAQQAGLKVGEFIWTGGDCHIYDNHREQVLTQLSREPRPYPQLTLR
KASSIFDYDFDDFTIEGYDPHPAIRGEVAV

Sequences:

>Translated_270_residues
MSTNNTIKTPYEDLLRTILEQGSHKDDRTGTGTTSLFGQQMRFDLTEAFPLITTKKVYWKGVIGELLWFLQGSSNVRWLQ
KRNIHIWDEWASEEGELGPVYGVQWRSWPTPDGQHVDQIAQALDILKNNPDSRRNIVSAWNVADLNNMALPPCHLLFQLY
VADGKLSCQLYQRSADMFLGVPFNIASYSALTHMLAQQAGLKVGEFIWTGGDCHIYDNHREQVLTQLSREPRPYPQLTLR
KASSIFDYDFDDFTIEGYDPHPAIRGEVAV
>Mature_269_residues
STNNTIKTPYEDLLRTILEQGSHKDDRTGTGTTSLFGQQMRFDLTEAFPLITTKKVYWKGVIGELLWFLQGSSNVRWLQK
RNIHIWDEWASEEGELGPVYGVQWRSWPTPDGQHVDQIAQALDILKNNPDSRRNIVSAWNVADLNNMALPPCHLLFQLYV
ADGKLSCQLYQRSADMFLGVPFNIASYSALTHMLAQQAGLKVGEFIWTGGDCHIYDNHREQVLTQLSREPRPYPQLTLRK
ASSIFDYDFDDFTIEGYDPHPAIRGEVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=53.7366548042705, Blast_Score=297, Evalue=6e-81,
Organism=Escherichia coli, GI1789191, Length=261, Percent_Identity=63.9846743295019, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71993377, Length=288, Percent_Identity=46.5277777777778, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6324648, Length=288, Percent_Identity=39.2361111111111, Blast_Score=192, Evalue=5e-50,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=51.2367491166078, Blast_Score=290, Evalue=7e-79,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_CORDI (Q6NIF2)

Other databases:

- EMBL:   BX248356
- RefSeq:   NP_939189.1
- ProteinModelPortal:   Q6NIF2
- SMR:   Q6NIF2
- GeneID:   2650540
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0825
- NMPDR:   fig|257309.1.peg.780
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   Q6NIF2
- ProtClustDB:   PRK01827
- BioCyc:   CDIP257309:DIP0825-MONOMER
- BRENDA:   2.1.1.45
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30835; Mature: 30704

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNNTIKTPYEDLLRTILEQGSHKDDRTGTGTTSLFGQQMRFDLTEAFPLITTKKVYWK
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
GVIGELLWFLQGSSNVRWLQKRNIHIWDEWASEEGELGPVYGVQWRSWPTPDGQHVDQIA
HHHHHHHHHHCCCCCEEEHHHCCCEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHH
QALDILKNNPDSRRNIVSAWNVADLNNMALPPCHLLFQLYVADGKLSCQLYQRSADMFLG
HHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHEECCCCCEEEE
VPFNIASYSALTHMLAQQAGLKVGEFIWTGGDCHIYDNHREQVLTQLSREPRPYPQLTLR
CCCCHHHHHHHHHHHHHHHCCCCHHEEEECCEEEEECCHHHHHHHHHHHCCCCCCHHHHH
KASSIFDYDFDDFTIEGYDPHPAIRGEVAV
HHHHHHCCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
STNNTIKTPYEDLLRTILEQGSHKDDRTGTGTTSLFGQQMRFDLTEAFPLITTKKVYWK
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
GVIGELLWFLQGSSNVRWLQKRNIHIWDEWASEEGELGPVYGVQWRSWPTPDGQHVDQIA
HHHHHHHHHHCCCCCEEEHHHCCCEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHH
QALDILKNNPDSRRNIVSAWNVADLNNMALPPCHLLFQLYVADGKLSCQLYQRSADMFLG
HHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHEECCCCCEEEE
VPFNIASYSALTHMLAQQAGLKVGEFIWTGGDCHIYDNHREQVLTQLSREPRPYPQLTLR
CCCCHHHHHHHHHHHHHHHCCCCHHEEEECCEEEEECCHHHHHHHHHHHCCCCCCHHHHH
KASSIFDYDFDDFTIEGYDPHPAIRGEVAV
HHHHHHCCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910