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The map label for this gene is sseA [H]

Identifier: 38233257

GI number: 38233257

Start: 624604

End: 625485

Strand: Reverse

Name: sseA [H]

Synonym: DIP0650

Alternate gene names: 38233257

Gene position: 625485-624604 (Counterclockwise)

Preceding gene: 38233260

Following gene: 38233256

Centisome position: 25.13

GC content: 57.48

Gene sequence:

>882_bases
ATGCCAGCACCATTCGATCCACACCCACAGTTCCAGGAGTACGCACACCCCGAAAAGCTCGTCTCCGCCTCGTGGCTGAG
CGCTCGCCTAGGCACTAAAGGCCTTCGCGTTGTGGAATCCGACGAGGACTCACTCCTCTACGACATCGGACACATCCCCG
GTGCCGTACGCATCGACTGGGCCAAAGACCTTAACGACGCCACCATCCGCGATTACATCAGCGCCAAGGATTTCGCAGCG
CTCATGGACGCTAAAGGCATCGCCCGAGACGACACCGTAGTGATCTACGGCGATAAGTCCAACTGGTGGGCAACCTTTAC
CCTATGGATCTTCGAGCTCTTCGGCCATGAGGATGTCCGTATCCTCAACGGTGGCCGAGACGCATGGATGGCCGAAGAAC
GCGACACCTCGTATGCCGTTCCGGAGTACCCCGCAACCGGCTACCCCGTTGTCACCGATACCAGCGCACCAGCGCGCATT
TTTGTCGACGAGCTCGTGAGCAAACTGGGCACGCAACAGCTTATCGACGTCCGCAGTGAATCCGAATATGCGGGCACCAC
AGACAATGATTACCCCTCATCTGGGGTGTTACGGAGCGGCCATATCCCAACCGCAGTGAACATTCCATGGAATAAGTCAG
TACACCCGAACAGCCGGTTCCGCTCCTTTTCCGAACTCAGTGAGATCTACGGTGGACTCGACAAAAATGCCGAAACCGTC
ACCTACTGTCAAGTAGGCGACCGCGCAGCACACACTTGGTTCGTCCTAAAGTACCTGCTTGGACACGACATTGCCCGCAC
TTACGACGGATCTTGGGCCGAATGGGGCAACATGATTCGCATGCCTATCGCGAAAGGCGAAGAGCCCGGCGTGGCACCCT
AG

Upstream 100 bases:

>100_bases
TAAAGGTGGGTCAACCCCGTGACTTTTCACGCCAGCCCACTGCCATAGTGGAATCTTTCAGCGATTGCGCGTCACCGTTT
AGTCATTGAAGAAGGTAAAA

Downstream 100 bases:

>100_bases
AAAAATAATAGTTTTGACTATAAAATGAATTCCTCGGGAGCAATTCTTCCGAGGAATTTGCCGTTCAATGGCTACCTAAT
GTGGAAGAATATGTGATGCC

Product: putative thiosulfate sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPAPFDPHPQFQEYAHPEKLVSASWLSARLGTKGLRVVESDEDSLLYDIGHIPGAVRIDWAKDLNDATIRDYISAKDFAA
LMDAKGIARDDTVVIYGDKSNWWATFTLWIFELFGHEDVRILNGGRDAWMAEERDTSYAVPEYPATGYPVVTDTSAPARI
FVDELVSKLGTQQLIDVRSESEYAGTTDNDYPSSGVLRSGHIPTAVNIPWNKSVHPNSRFRSFSELSEIYGGLDKNAETV
TYCQVGDRAAHTWFVLKYLLGHDIARTYDGSWAEWGNMIRMPIAKGEEPGVAP

Sequences:

>Translated_293_residues
MPAPFDPHPQFQEYAHPEKLVSASWLSARLGTKGLRVVESDEDSLLYDIGHIPGAVRIDWAKDLNDATIRDYISAKDFAA
LMDAKGIARDDTVVIYGDKSNWWATFTLWIFELFGHEDVRILNGGRDAWMAEERDTSYAVPEYPATGYPVVTDTSAPARI
FVDELVSKLGTQQLIDVRSESEYAGTTDNDYPSSGVLRSGHIPTAVNIPWNKSVHPNSRFRSFSELSEIYGGLDKNAETV
TYCQVGDRAAHTWFVLKYLLGHDIARTYDGSWAEWGNMIRMPIAKGEEPGVAP
>Mature_292_residues
PAPFDPHPQFQEYAHPEKLVSASWLSARLGTKGLRVVESDEDSLLYDIGHIPGAVRIDWAKDLNDATIRDYISAKDFAAL
MDAKGIARDDTVVIYGDKSNWWATFTLWIFELFGHEDVRILNGGRDAWMAEERDTSYAVPEYPATGYPVVTDTSAPARIF
VDELVSKLGTQQLIDVRSESEYAGTTDNDYPSSGVLRSGHIPTAVNIPWNKSVHPNSRFRSFSELSEIYGGLDKNAETVT
YCQVGDRAAHTWFVLKYLLGHDIARTYDGSWAEWGNMIRMPIAKGEEPGVAP

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=277, Percent_Identity=28.158844765343, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI61835204, Length=270, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI194473681, Length=270, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI194473668, Length=269, Percent_Identity=27.8810408921933, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI87082121, Length=278, Percent_Identity=27.3381294964029, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI87081967, Length=306, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115534702, Length=254, Percent_Identity=25.5905511811024, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 32721; Mature: 32589

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPFDPHPQFQEYAHPEKLVSASWLSARLGTKGLRVVESDEDSLLYDIGHIPGAVRIDW
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCEEEEEH
AKDLNDATIRDYISAKDFAALMDAKGIARDDTVVIYGDKSNWWATFTLWIFELFGHEDVR
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHCCCCEE
ILNGGRDAWMAEERDTSYAVPEYPATGYPVVTDTSAPARIFVDELVSKLGTQQLIDVRSE
EEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCHHHHHHHCCC
SEYAGTTDNDYPSSGVLRSGHIPTAVNIPWNKSVHPNSRFRSFSELSEIYGGLDKNAETV
CCCCCCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
TYCQVGDRAAHTWFVLKYLLGHDIARTYDGSWAEWGNMIRMPIAKGEEPGVAP
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
PAPFDPHPQFQEYAHPEKLVSASWLSARLGTKGLRVVESDEDSLLYDIGHIPGAVRIDW
CCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCEEEEEH
AKDLNDATIRDYISAKDFAALMDAKGIARDDTVVIYGDKSNWWATFTLWIFELFGHEDVR
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHCCCCEE
ILNGGRDAWMAEERDTSYAVPEYPATGYPVVTDTSAPARIFVDELVSKLGTQQLIDVRSE
EEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCHHHHHHHCCC
SEYAGTTDNDYPSSGVLRSGHIPTAVNIPWNKSVHPNSRFRSFSELSEIYGGLDKNAETV
CCCCCCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
TYCQVGDRAAHTWFVLKYLLGHDIARTYDGSWAEWGNMIRMPIAKGEEPGVAP
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626; 8772169 [H]