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The map label for this gene is nagB

Identifier: 38233130

GI number: 38233130

Start: 489788

End: 490567

Strand: Direct

Name: nagB

Synonym: DIP0521

Alternate gene names: 38233130

Gene position: 489788-490567 (Clockwise)

Preceding gene: 38233129

Following gene: 38233133

Centisome position: 19.68

GC content: 52.56

Gene sequence:

>780_bases
ATGGAGATCGTAATCACCCCCACCAAGGACGACGCCGCTCGTATCGCCGCCGACATCCTAGAAGAATACGCACGTGAAGG
AAAGACCCTGGGTCTTGCTACCGGATCTACTCCGCTAGGCACCTACCAAGAGCTCATTCGTCGCCACAATGAGGAAGGCT
TGTCGTTTGCCGAATGCCAAGCATTTACTCTCGACGAATACGTCGGATTGCCTCGCGAACACGAACAAAGCTACTACAGC
ACCATTCGACGGGAATTTACTTCGCATATCGATATCCCAGACGAGAAGGTGTTCAACCCTGACGGCACCGCAGAACACCC
AGGACAAGCAGCACAGGAATACGATCGCCTGATCGTAGAAAAAGGCGGTGTTGACATTCAGATTCTTGGTATCGGAACCG
ATGGTCACATTGCTTTTAACGAGCCAACCTCGTCTATGGCAAGCCGTACGCGTATCAAGACGCTGCACCCAGACACGGTA
CGCGATAATTCTCGATTCTTTAATGGCAACGAATCACAAGTTCCACACCACGTGATGACCCAAGGAATCGGCACCATTCG
CGAGGCCCGACACCTGCTGATGCTCTGCTTTGGCGAGAATAAAGCAGATGCAGTCAAAGCCATGGTGGAAGGGCCAGTCG
CAGCCGTGTGCCCAGCATCTGTTTTGCAGCTGCATGAGCATGCCACTGTGATTGTTGATGAGGCAGCGGCAGCAAAACTT
GAGCACACTCAGTACTACCGCTATGCGCTAGAGAATAAGCCAGACTGGCAACGCTTCTAA

Upstream 100 bases:

>100_bases
AGACCTTGTCCTATGCAGCGGCGACTTCCATGTCGACCACGTGCTCATCAAAGGCGAGGACATCTAAGGCCACAAGCCTC
CTCAAAGGAAAGAGAAAACC

Downstream 100 bases:

>100_bases
TCGCATAGAATAAGGAGCGCTCCTCTTTGGTGAGGGGAGCGCTCCTTTTTACGTTATTGATTAACGAATACCTGGGATTC
GAATGTTGAAGATCTTAGCC

Product: putative isomerase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MEIVITPTKDDAARIAADILEEYAREGKTLGLATGSTPLGTYQELIRRHNEEGLSFAECQAFTLDEYVGLPREHEQSYYS
TIRREFTSHIDIPDEKVFNPDGTAEHPGQAAQEYDRLIVEKGGVDIQILGIGTDGHIAFNEPTSSMASRTRIKTLHPDTV
RDNSRFFNGNESQVPHHVMTQGIGTIREARHLLMLCFGENKADAVKAMVEGPVAAVCPASVLQLHEHATVIVDEAAAAKL
EHTQYYRYALENKPDWQRF

Sequences:

>Translated_259_residues
MEIVITPTKDDAARIAADILEEYAREGKTLGLATGSTPLGTYQELIRRHNEEGLSFAECQAFTLDEYVGLPREHEQSYYS
TIRREFTSHIDIPDEKVFNPDGTAEHPGQAAQEYDRLIVEKGGVDIQILGIGTDGHIAFNEPTSSMASRTRIKTLHPDTV
RDNSRFFNGNESQVPHHVMTQGIGTIREARHLLMLCFGENKADAVKAMVEGPVAAVCPASVLQLHEHATVIVDEAAAAKL
EHTQYYRYALENKPDWQRF
>Mature_259_residues
MEIVITPTKDDAARIAADILEEYAREGKTLGLATGSTPLGTYQELIRRHNEEGLSFAECQAFTLDEYVGLPREHEQSYYS
TIRREFTSHIDIPDEKVFNPDGTAEHPGQAAQEYDRLIVEKGGVDIQILGIGTDGHIAFNEPTSSMASRTRIKTLHPDTV
RDNSRFFNGNESQVPHHVMTQGIGTIREARHLLMLCFGENKADAVKAMVEGPVAAVCPASVLQLHEHATVIVDEAAAAKL
EHTQYYRYALENKPDWQRF

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI19923881, Length=256, Percent_Identity=39.84375, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI13027378, Length=221, Percent_Identity=42.0814479638009, Blast_Score=179, Evalue=2e-45,
Organism=Escherichia coli, GI1786893, Length=213, Percent_Identity=44.6009389671362, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI48994958, Length=242, Percent_Identity=30.1652892561983, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1789530, Length=210, Percent_Identity=26.1904761904762, Blast_Score=85, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17554876, Length=255, Percent_Identity=41.9607843137255, Blast_Score=202, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24581960, Length=261, Percent_Identity=38.6973180076628, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI19920764, Length=261, Percent_Identity=38.6973180076628, Blast_Score=183, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_CORDI (Q6NJ91)

Other databases:

- EMBL:   BX248355
- RefSeq:   NP_938897.1
- HSSP:   P0A759
- ProteinModelPortal:   Q6NJ91
- SMR:   Q6NJ91
- GeneID:   2648737
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0521
- NMPDR:   fig|257309.1.peg.488
- HOGENOM:   HBG725991
- OMA:   HLSITMG
- PhylomeDB:   Q6NJ91
- ProtClustDB:   CLSK2503064
- BioCyc:   CDIP257309:DIP0521-MONOMER
- BRENDA:   3.5.99.6
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 28905; Mature: 28905

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 65-65 ACT_SITE 134-134 ACT_SITE 136-136 ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIVITPTKDDAARIAADILEEYAREGKTLGLATGSTPLGTYQELIRRHNEEGLSFAECQ
CEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHH
AFTLDEYVGLPREHEQSYYSTIRREFTSHIDIPDEKVFNPDGTAEHPGQAAQEYDRLIVE
EEEHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
KGGVDIQILGIGTDGHIAFNEPTSSMASRTRIKTLHPDTVRDNSRFFNGNESQVPHHVMT
CCCCEEEEEEECCCCCEEECCCHHHHHHHHHHEECCCCCCCCCCCEECCCCCCCCHHHHH
QGIGTIREARHLLMLCFGENKADAVKAMVEGPVAAVCPASVLQLHEHATVIVDEAAAAKL
HHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECHHHHHH
EHTQYYRYALENKPDWQRF
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEIVITPTKDDAARIAADILEEYAREGKTLGLATGSTPLGTYQELIRRHNEEGLSFAECQ
CEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHH
AFTLDEYVGLPREHEQSYYSTIRREFTSHIDIPDEKVFNPDGTAEHPGQAAQEYDRLIVE
EEEHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
KGGVDIQILGIGTDGHIAFNEPTSSMASRTRIKTLHPDTVRDNSRFFNGNESQVPHHVMT
CCCCEEEEEEECCCCCEEECCCHHHHHHHHHHEECCCCCCCCCCCEECCCCCCCCHHHHH
QGIGTIREARHLLMLCFGENKADAVKAMVEGPVAAVCPASVLQLHEHATVIVDEAAAAKL
HHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECHHHHHH
EHTQYYRYALENKPDWQRF
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910