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The map label for this gene is narK [H]

Identifier: 38233113

GI number: 38233113

Start: 470374

End: 471705

Strand: Reverse

Name: narK [H]

Synonym: DIP0501

Alternate gene names: 38233113

Gene position: 471705-470374 (Counterclockwise)

Preceding gene: 38233114

Following gene: 38233112

Centisome position: 18.95

GC content: 52.93

Gene sequence:

>1332_bases
ATGGCAACCATTAATACTTCTGGTCGAGTCCTAACTGGCTGGAACCCAGAAGATCCCGAAGCTTGGGACTCTAAGATTGC
GTGGCGCACCCTCGTCATCACTACATACTCATTGTTCATTGGTTTTTCCACGTGGTACTTGGTATCCGCCATTGCACCAC
AGCTCAACCAAATCGGATTCAATTTCTCCAAAACGCAGTTGTACTGGCTGTCTTCCGCATCGGGCCTAGCAGCAGGCTTC
TCTCGCTTAGCGTTTATGTTCCTGCCACCTATCGTCGGTACGCGCAAGCTAGTCACCATCTCCTCCTTTGTTCTCGTCCT
GCCTATGTTGGGCTGGTTCTTTGCCATCATCAATCCAGCAACACCGTACTGGTGGATGCTTGTTCTCTCTGTTGCAGGCG
GCCTCGGCGGCGGTGTGTTCTCCGGCTACATGCCTTCCACCGGCTATTTCTTCCCCAAAGCAAAGGCTGGTACAGCCCTT
GGCTTACAAGCCGGTATCGGCAACTTCGGTGTGTCCTTCATTCAGCTCGCAGCACCACTACTGATGGGTGTGTCCTTGCT
CGGTGTCAGTGCCAAGCCACAATTGCGCACAGATGGTAGCGAGGTATTTGTTCACACCCCAGCAATCTTCATGGTTCCAT
GGACCATCCTCGCTGGTGTTCTGGCATGGATGTATCTGAAAGATGTTCCAGTCAAAGCAAACTTCCGTCAGCAAATGGAC
ATCTTTGGCAACTTCAACACCTGGATCATGACCCTTATCTACTTGGCCACCTTCGGCGCATTTTCCGGTTTTGCTGCCCA
ATTCGCGCTGTTGGTCAATAACATCTACGGTGCCAATTCTTCACTGGCTGATACTTTTGCTGCTAGTGAGTTGCCTAAGG
GCGCTGCTTTCGCCTTCCTCGGGCCACTGATTTCTGCTGCTGTCCGTGCTGCATGGGGCCCATTCTGTGACCGCTTCGGT
GGTGCGATCTGGACCTTTATTTCCTGCGCCGGTATGGCAGTCTCCACTGCTGTTGCTGCACTCCTCCTTAATCCAACCGA
TCCAAGCCAGTTCTGGTACTTCCTCGCAGCAATGCTGACCATGTTCTTCTTTACTGGTCTTGGCAACGCCGGCACGTTCA
AACAAATGCCGATGATTTTCCCCCGTCGTCAAGCAGGTGGCGTTATTGGTTGGACTGCAGCAATCGCCGCCTTCGGCCCA
TTCTTTGTCGGCGTTATTGTTTCCACCCTTGGTGCACCAGCCTTCTTCTGGGGTTGCGTTGTGTTCTTTGCTCTGTGCGC
TGCACTGACTTGGATCTTCTACGCACGCCCTCAGGCACCATTCCCAGGCTAA

Upstream 100 bases:

>100_bases
TAGCTTTCACCATATGCAGAAAATCTGTAGCCTCAGACACTGCACTTATTAATTGTTATCCCCGTCTCACATTCTCGCGG
CGAGAAAGAAAAACTGTTTT

Downstream 100 bases:

>100_bases
CAACCTGATCTCATTCTCACCCATTGCTACATAAGGAATACTGATGAGTGACCCAAACTCAGTTAATCCGCTCTTTAAGC
TTGGAAGCTTTGTACGCAAA

Product: putative nitrate/nitrite transport system integral membrane protein

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MATINTSGRVLTGWNPEDPEAWDSKIAWRTLVITTYSLFIGFSTWYLVSAIAPQLNQIGFNFSKTQLYWLSSASGLAAGF
SRLAFMFLPPIVGTRKLVTISSFVLVLPMLGWFFAIINPATPYWWMLVLSVAGGLGGGVFSGYMPSTGYFFPKAKAGTAL
GLQAGIGNFGVSFIQLAAPLLMGVSLLGVSAKPQLRTDGSEVFVHTPAIFMVPWTILAGVLAWMYLKDVPVKANFRQQMD
IFGNFNTWIMTLIYLATFGAFSGFAAQFALLVNNIYGANSSLADTFAASELPKGAAFAFLGPLISAAVRAAWGPFCDRFG
GAIWTFISCAGMAVSTAVAALLLNPTDPSQFWYFLAAMLTMFFFTGLGNAGTFKQMPMIFPRRQAGGVIGWTAAIAAFGP
FFVGVIVSTLGAPAFFWGCVVFFALCAALTWIFYARPQAPFPG

Sequences:

>Translated_443_residues
MATINTSGRVLTGWNPEDPEAWDSKIAWRTLVITTYSLFIGFSTWYLVSAIAPQLNQIGFNFSKTQLYWLSSASGLAAGF
SRLAFMFLPPIVGTRKLVTISSFVLVLPMLGWFFAIINPATPYWWMLVLSVAGGLGGGVFSGYMPSTGYFFPKAKAGTAL
GLQAGIGNFGVSFIQLAAPLLMGVSLLGVSAKPQLRTDGSEVFVHTPAIFMVPWTILAGVLAWMYLKDVPVKANFRQQMD
IFGNFNTWIMTLIYLATFGAFSGFAAQFALLVNNIYGANSSLADTFAASELPKGAAFAFLGPLISAAVRAAWGPFCDRFG
GAIWTFISCAGMAVSTAVAALLLNPTDPSQFWYFLAAMLTMFFFTGLGNAGTFKQMPMIFPRRQAGGVIGWTAAIAAFGP
FFVGVIVSTLGAPAFFWGCVVFFALCAALTWIFYARPQAPFPG
>Mature_442_residues
ATINTSGRVLTGWNPEDPEAWDSKIAWRTLVITTYSLFIGFSTWYLVSAIAPQLNQIGFNFSKTQLYWLSSASGLAAGFS
RLAFMFLPPIVGTRKLVTISSFVLVLPMLGWFFAIINPATPYWWMLVLSVAGGLGGGVFSGYMPSTGYFFPKAKAGTALG
LQAGIGNFGVSFIQLAAPLLMGVSLLGVSAKPQLRTDGSEVFVHTPAIFMVPWTILAGVLAWMYLKDVPVKANFRQQMDI
FGNFNTWIMTLIYLATFGAFSGFAAQFALLVNNIYGANSSLADTFAASELPKGAAFAFLGPLISAAVRAAWGPFCDRFGG
AIWTFISCAGMAVSTAVAALLLNPTDPSQFWYFLAAMLTMFFFTGLGNAGTFKQMPMIFPRRQAGGVIGWTAAIAAFGPF
FVGVIVSTLGAPAFFWGCVVFFALCAALTWIFYARPQAPFPG

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=465, Percent_Identity=32.4731182795699, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1787743, Length=469, Percent_Identity=32.8358208955224, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47700; Mature: 47569

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATINTSGRVLTGWNPEDPEAWDSKIAWRTLVITTYSLFIGFSTWYLVSAIAPQLNQIGF
CCEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NFSKTQLYWLSSASGLAAGFSRLAFMFLPPIVGTRKLVTISSFVLVLPMLGWFFAIINPA
CCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
TPYWWMLVLSVAGGLGGGVFSGYMPSTGYFFPKAKAGTALGLQAGIGNFGVSFIQLAAPL
CHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
LMGVSLLGVSAKPQLRTDGSEVFVHTPAIFMVPWTILAGVLAWMYLKDVPVKANFRQQMD
HHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
IFGNFNTWIMTLIYLATFGAFSGFAAQFALLVNNIYGANSSLADTFAASELPKGAAFAFL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHH
GPLISAAVRAAWGPFCDRFGGAIWTFISCAGMAVSTAVAALLLNPTDPSQFWYFLAAMLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MFFFTGLGNAGTFKQMPMIFPRRQAGGVIGWTAAIAAFGPFFVGVIVSTLGAPAFFWGCV
HHHHHCCCCCCCHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VFFALCAALTWIFYARPQAPFPG
HHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ATINTSGRVLTGWNPEDPEAWDSKIAWRTLVITTYSLFIGFSTWYLVSAIAPQLNQIGF
CEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NFSKTQLYWLSSASGLAAGFSRLAFMFLPPIVGTRKLVTISSFVLVLPMLGWFFAIINPA
CCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
TPYWWMLVLSVAGGLGGGVFSGYMPSTGYFFPKAKAGTALGLQAGIGNFGVSFIQLAAPL
CHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
LMGVSLLGVSAKPQLRTDGSEVFVHTPAIFMVPWTILAGVLAWMYLKDVPVKANFRQQMD
HHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
IFGNFNTWIMTLIYLATFGAFSGFAAQFALLVNNIYGANSSLADTFAASELPKGAAFAFL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHH
GPLISAAVRAAWGPFCDRFGGAIWTFISCAGMAVSTAVAALLLNPTDPSQFWYFLAAMLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MFFFTGLGNAGTFKQMPMIFPRRQAGGVIGWTAAIAAFGPFFVGVIVSTLGAPAFFWGCV
HHHHHCCCCCCCHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VFFALCAALTWIFYARPQAPFPG
HHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]