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The map label for this gene is narK [H]

Identifier: 38233114

GI number: 38233114

Start: 472039

End: 473337

Strand: Reverse

Name: narK [H]

Synonym: DIP0502

Alternate gene names: 38233114

Gene position: 473337-472039 (Counterclockwise)

Preceding gene: 38233116

Following gene: 38233113

Centisome position: 19.02

GC content: 54.04

Gene sequence:

>1299_bases
ATGAGTCACCCTCCTATCCACGGCGAGAATGCCTCGTCGCCCGAGCTCGAACCAACCCCCCAGGGCGCCAATTCCGTCCT
CATCTTCTCCACCGTGTCCTTTACCATGATGTTTGCCGTCTGGCTCATGTTTGGCATCTTGGGCACTCCCATCCAAAAGG
AATTTGGATTAAGCGACACTCAGCTTTCATGGATCCTGGCCGTTGCCATCCTTAACGGATCAATGTGGCGCCTACCAGCA
GGCATCTTGGCCGACAAGATTGGTGGCCGCAAGGTAATGCTGGGCATCACGCTCTTTTCTGCGGTAGCTTCCCTCGGCGT
TGCGTTCTCACAAAACTACACCATGACCCTAGTGCTGGCCTTCTTGGTGGGATTCTGCGGCAATTCTTTCACAGCAGGTG
CAGCGTGGGCATCCGCTTGGTTCCCTAAGCACAAGCAGGGTTTTGCCCTTGGCGTTTTCGGCGCCGGCAACGTTGGCGCC
TCCGTCACTAAATTCATCGGCCCTGTGATCATTGCTAGCACCGCAGGTGCAACATACTTTGGCTTTATCCCAGCAGGTTG
GCGTCTCATCCCTATCATCTACAGCGTCACCCTAGTCATTGTTGCCATCCTCATGATGATTTACACCCCCAAGGTTGATC
GTTTTGGCAGCTCTGGTCGTAGCTTGGCAGAGATGCTTCTTCCTCTCAAGCAGGTGCGAGTATGGCGTTTTAGCCTCTAC
TACGTCGTTGTTTTCGGTGCTTATGTTGCTCTGTCTGCAACTTTGCCCAAGTACTACATCTCTAACTATGACGTAAGTCT
TGCCGCTGCTGGTCTACTCACAGCAATCTTCATCTTCCCGGCATCCCTGCTGCGTCCAGTCGGTGGTTGGTTCTCCGATC
GCTACGGTGCTCGCCGCGCTATGTATGGCACTTTCTTTGTTATGGGTACCGCAGCAGGCCTACTCTGCCTTCCCAGTAAC
ATCATCAACATCTTCGTCTTTACCCTTTTGATTTTCTCCCTTGGCTGCGCCATGGGCGTGGGCAAAGCCGCAGTATATAA
GCACATCCCAGAATACTTCCCGCATGACGTTGGAGCCGTTGGCGGCCTGGTGGGCATGTTGGGCGGCTTGGGCGGTTTCT
TCCTCCCACCACTATTTGCTTACACCAAGGCGTGGACTGGATGGAGCACCAGCCCATTCATCGTGTTGCTCATCTTGGTA
GCGGTGTCTGCACTGTGGATGCACGTAACGGTTCACCGCATGTTACACAGCCATGTTCCCGAGCTGAAACACCACATGGA
GGTGCCAGCCACTCAATAA

Upstream 100 bases:

>100_bases
CTACTTAATCCCTCGTATTCTTTTATCTCCACATTGTGCGCAATACGTTTGTATGCCAATAAACACCGTGCACAAGCGGA
CATTTCCAAGAAAGCGCATT

Downstream 100 bases:

>100_bases
AATTGAAAAATATTATCAGAATATCCGGTTACGCAAGATGAGTTTTGCCTCACGTAACCGGATATTCTTTTGGTTTCACC
CCACGACTATGAGAAAAAAC

Product: putative nitrate/nitrite transport system integral membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSHPPIHGENASSPELEPTPQGANSVLIFSTVSFTMMFAVWLMFGILGTPIQKEFGLSDTQLSWILAVAILNGSMWRLPA
GILADKIGGRKVMLGITLFSAVASLGVAFSQNYTMTLVLAFLVGFCGNSFTAGAAWASAWFPKHKQGFALGVFGAGNVGA
SVTKFIGPVIIASTAGATYFGFIPAGWRLIPIIYSVTLVIVAILMMIYTPKVDRFGSSGRSLAEMLLPLKQVRVWRFSLY
YVVVFGAYVALSATLPKYYISNYDVSLAAAGLLTAIFIFPASLLRPVGGWFSDRYGARRAMYGTFFVMGTAAGLLCLPSN
IINIFVFTLLIFSLGCAMGVGKAAVYKHIPEYFPHDVGAVGGLVGMLGGLGGFFLPPLFAYTKAWTGWSTSPFIVLLILV
AVSALWMHVTVHRMLHSHVPELKHHMEVPATQ

Sequences:

>Translated_432_residues
MSHPPIHGENASSPELEPTPQGANSVLIFSTVSFTMMFAVWLMFGILGTPIQKEFGLSDTQLSWILAVAILNGSMWRLPA
GILADKIGGRKVMLGITLFSAVASLGVAFSQNYTMTLVLAFLVGFCGNSFTAGAAWASAWFPKHKQGFALGVFGAGNVGA
SVTKFIGPVIIASTAGATYFGFIPAGWRLIPIIYSVTLVIVAILMMIYTPKVDRFGSSGRSLAEMLLPLKQVRVWRFSLY
YVVVFGAYVALSATLPKYYISNYDVSLAAAGLLTAIFIFPASLLRPVGGWFSDRYGARRAMYGTFFVMGTAAGLLCLPSN
IINIFVFTLLIFSLGCAMGVGKAAVYKHIPEYFPHDVGAVGGLVGMLGGLGGFFLPPLFAYTKAWTGWSTSPFIVLLILV
AVSALWMHVTVHRMLHSHVPELKHHMEVPATQ
>Mature_431_residues
SHPPIHGENASSPELEPTPQGANSVLIFSTVSFTMMFAVWLMFGILGTPIQKEFGLSDTQLSWILAVAILNGSMWRLPAG
ILADKIGGRKVMLGITLFSAVASLGVAFSQNYTMTLVLAFLVGFCGNSFTAGAAWASAWFPKHKQGFALGVFGAGNVGAS
VTKFIGPVIIASTAGATYFGFIPAGWRLIPIIYSVTLVIVAILMMIYTPKVDRFGSSGRSLAEMLLPLKQVRVWRFSLYY
VVVFGAYVALSATLPKYYISNYDVSLAAAGLLTAIFIFPASLLRPVGGWFSDRYGARRAMYGTFFVMGTAAGLLCLPSNI
INIFVFTLLIFSLGCAMGVGKAAVYKHIPEYFPHDVGAVGGLVGMLGGLGGFFLPPLFAYTKAWTGWSTSPFIVLLILVA
VSALWMHVTVHRMLHSHVPELKHHMEVPATQ

Specific function: Probably required for nitrate uptake under anoxic conditions. Also possibly involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=412, Percent_Identity=25.7281553398058, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787743, Length=442, Percent_Identity=25.1131221719457, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46534; Mature: 46402

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHPPIHGENASSPELEPTPQGANSVLIFSTVSFTMMFAVWLMFGILGTPIQKEFGLSDT
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHH
QLSWILAVAILNGSMWRLPAGILADKIGGRKVMLGITLFSAVASLGVAFSQNYTMTLVLA
HHHHHHHHHHHCCCHHHCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHH
FLVGFCGNSFTAGAAWASAWFPKHKQGFALGVFGAGNVGASVTKFIGPVIIASTAGATYF
HHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHEECCCCCEE
GFIPAGWRLIPIIYSVTLVIVAILMMIYTPKVDRFGSSGRSLAEMLLPLKQVRVWRFSLY
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
YVVVFGAYVALSATLPKYYISNYDVSLAAAGLLTAIFIFPASLLRPVGGWFSDRYGARRA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MYGTFFVMGTAAGLLCLPSNIINIFVFTLLIFSLGCAMGVGKAAVYKHIPEYFPHDVGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHH
GGLVGMLGGLGGFFLPPLFAYTKAWTGWSTSPFIVLLILVAVSALWMHVTVHRMLHSHVP
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKHHMEVPATQ
HHHHHCCCCCCC
>Mature Secondary Structure 
SHPPIHGENASSPELEPTPQGANSVLIFSTVSFTMMFAVWLMFGILGTPIQKEFGLSDT
CCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHH
QLSWILAVAILNGSMWRLPAGILADKIGGRKVMLGITLFSAVASLGVAFSQNYTMTLVLA
HHHHHHHHHHHCCCHHHCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHH
FLVGFCGNSFTAGAAWASAWFPKHKQGFALGVFGAGNVGASVTKFIGPVIIASTAGATYF
HHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHEECCCCCEE
GFIPAGWRLIPIIYSVTLVIVAILMMIYTPKVDRFGSSGRSLAEMLLPLKQVRVWRFSLY
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
YVVVFGAYVALSATLPKYYISNYDVSLAAAGLLTAIFIFPASLLRPVGGWFSDRYGARRA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MYGTFFVMGTAAGLLCLPSNIINIFVFTLLIFSLGCAMGVGKAAVYKHIPEYFPHDVGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHH
GGLVGMLGGLGGFFLPPLFAYTKAWTGWSTSPFIVLLILVAVSALWMHVTVHRMLHSHVP
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKHHMEVPATQ
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9082911; 12426351 [H]