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The map label for this gene is ansA [H]

Identifier: 38233103

GI number: 38233103

Start: 456732

End: 457760

Strand: Direct

Name: ansA [H]

Synonym: DIP0491

Alternate gene names: 38233103

Gene position: 456732-457760 (Clockwise)

Preceding gene: 38233102

Following gene: 38233115

Centisome position: 18.35

GC content: 60.16

Gene sequence:

>1029_bases
ATGATTTCACGCAGAAGTTTCCTCGGCGCATGCGCTCTTGCTTCCGTAGCAGGTGCGGTGCAGACATGCAGCAACAAAGC
CGGCAGCGCAGGTGTGCGTAACGACGCCTCCGGTCACCTCATCGTGCTCGGCACCGGTGGCACGATCGCATGCACCAACG
TCGATGGGGCACTGGTGCCCACCGTCTCCGGAGAAGAACTCGTCGCAGGAGTATACGACACCTTCGACAAAGACAAACTC
AGCATCGATGTCCGCCAAGTATCTCAGCTCGACTCCTCGGCAATGACACTCAAAGACACCGACATGATTATCACCGAGGT
ACTCAAAGCCGTAAAAGAAGACGGCGTGACCGGTGTGATCGTCACCCACGGCACCGACTCCATGGAAGAATCAGCCATCG
CCGTAGACACCTTCCTCGACTCCGACGTACCCGTCGTATTCACCGGCTCCATGCTGCCTTTCGACGACCCCAAAACCGAT
GGCCCAGCCAACCTCTTGCTCGCAGTTCGCACCGCTACCGCCCCCGAAAACCGCGGCAAAGGCGCGTTTATTGCCTTCGG
TGAAAAGACACTTCGTGCCCGTGGCGCCTACAAATCCAACGCCAATAGCGTGGACGGATTTGATAGCAACGCCGACAACG
AGCTCACCCGTCCTGCAGCATTGGCCTACAAACCCCTAGAACACCAGCGCGTTGACATCATCGCCGCCTACCCAGGCGCA
CCCCGCGCGCTTGTCGACGCCTCCCTCGCCAGCGGCGCCAAAGCCCTGGTCATCGAAGGCATGGGTGCCGGCAACGTAGG
CGGTGACATCGCCCTCGCTATCTCCGACGCCTTGGACAAGAAAATCCCCGTAGTGATGTCCACGCGTGTCGACGCCGGCC
GCGTCGAAGGCACCTACGGCGGTGCCGGCGGTGGCGCAACACTTGCCGCCAAAGGCGTGATTGGTGCAGACATCCTGCGC
GCCGGGCAGTCACGAATCCTACTGGCCTGCGCATTAGCAACGAAAACTGACCCGAAGACGCTGTTTTAG

Upstream 100 bases:

>100_bases
ACGACTTATGCCGTGTAGCTGATAAACCCCCTGCTGCCGATTGCCTCATCATGAGGTGGTCGGCAGTTTTCCCATATCCC
ACTTTCAAGGAGTATTCCTT

Downstream 100 bases:

>100_bases
GCACTCCATTGGTTGTAGCCACCTGATAGGCTCACAAGGTCTTTAACGCCGGCGCGGGAAAGCAGAATACATGCCCGCGC
CGAGCGTATTCCTGCGGCGC

Product: putative secreted amino acid hydrolase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MISRRSFLGACALASVAGAVQTCSNKAGSAGVRNDASGHLIVLGTGGTIACTNVDGALVPTVSGEELVAGVYDTFDKDKL
SIDVRQVSQLDSSAMTLKDTDMIITEVLKAVKEDGVTGVIVTHGTDSMEESAIAVDTFLDSDVPVVFTGSMLPFDDPKTD
GPANLLLAVRTATAPENRGKGAFIAFGEKTLRARGAYKSNANSVDGFDSNADNELTRPAALAYKPLEHQRVDIIAAYPGA
PRALVDASLASGAKALVIEGMGAGNVGGDIALAISDALDKKIPVVMSTRVDAGRVEGTYGGAGGGATLAAKGVIGADILR
AGQSRILLACALATKTDPKTLF

Sequences:

>Translated_342_residues
MISRRSFLGACALASVAGAVQTCSNKAGSAGVRNDASGHLIVLGTGGTIACTNVDGALVPTVSGEELVAGVYDTFDKDKL
SIDVRQVSQLDSSAMTLKDTDMIITEVLKAVKEDGVTGVIVTHGTDSMEESAIAVDTFLDSDVPVVFTGSMLPFDDPKTD
GPANLLLAVRTATAPENRGKGAFIAFGEKTLRARGAYKSNANSVDGFDSNADNELTRPAALAYKPLEHQRVDIIAAYPGA
PRALVDASLASGAKALVIEGMGAGNVGGDIALAISDALDKKIPVVMSTRVDAGRVEGTYGGAGGGATLAAKGVIGADILR
AGQSRILLACALATKTDPKTLF
>Mature_342_residues
MISRRSFLGACALASVAGAVQTCSNKAGSAGVRNDASGHLIVLGTGGTIACTNVDGALVPTVSGEELVAGVYDTFDKDKL
SIDVRQVSQLDSSAMTLKDTDMIITEVLKAVKEDGVTGVIVTHGTDSMEESAIAVDTFLDSDVPVVFTGSMLPFDDPKTD
GPANLLLAVRTATAPENRGKGAFIAFGEKTLRARGAYKSNANSVDGFDSNADNELTRPAALAYKPLEHQRVDIIAAYPGA
PRALVDASLASGAKALVIEGMGAGNVGGDIALAISDALDKKIPVVMSTRVDAGRVEGTYGGAGGGATLAAKGVIGADILR
AGQSRILLACALATKTDPKTLF

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788065, Length=327, Percent_Identity=28.4403669724771, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789327, Length=251, Percent_Identity=29.0836653386454, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17558278, Length=330, Percent_Identity=24.8484848484848, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17558280, Length=330, Percent_Identity=24.8484848484848, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193207374, Length=330, Percent_Identity=24.8484848484848, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193207372, Length=330, Percent_Identity=24.8484848484848, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323189, Length=330, Percent_Identity=29.0909090909091, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323187, Length=330, Percent_Identity=29.0909090909091, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323186, Length=330, Percent_Identity=29.0909090909091, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323184, Length=330, Percent_Identity=29.0909090909091, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320527, Length=325, Percent_Identity=28.3076923076923, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24645530, Length=256, Percent_Identity=30.46875, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24639609, Length=259, Percent_Identity=26.2548262548263, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 34899; Mature: 34899

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRRSFLGACALASVAGAVQTCSNKAGSAGVRNDASGHLIVLGTGGTIACTNVDGALVP
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCEEEC
TVSGEELVAGVYDTFDKDKLSIDVRQVSQLDSSAMTLKDTDMIITEVLKAVKEDGVTGVI
CCCCCHHHHHHHHCCCCCCEEEEHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCEEEE
VTHGTDSMEESAIAVDTFLDSDVPVVFTGSMLPFDDPKTDGPANLLLAVRTATAPENRGK
EECCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
GAFIAFGEKTLRARGAYKSNANSVDGFDSNADNELTRPAALAYKPLEHQRVDIIAAYPGA
EEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCCCEEEEEECCCC
PRALVDASLASGAKALVIEGMGAGNVGGDIALAISDALDKKIPVVMSTRVDAGRVEGTYG
CHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEHHHCCCCCEEEEECCCCCCCCCCCC
GAGGGATLAAKGVIGADILRAGQSRILLACALATKTDPKTLF
CCCCCCEEEECCCHHHHHHHCCCCEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MISRRSFLGACALASVAGAVQTCSNKAGSAGVRNDASGHLIVLGTGGTIACTNVDGALVP
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCEEEC
TVSGEELVAGVYDTFDKDKLSIDVRQVSQLDSSAMTLKDTDMIITEVLKAVKEDGVTGVI
CCCCCHHHHHHHHCCCCCCEEEEHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCEEEE
VTHGTDSMEESAIAVDTFLDSDVPVVFTGSMLPFDDPKTDGPANLLLAVRTATAPENRGK
EECCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
GAFIAFGEKTLRARGAYKSNANSVDGFDSNADNELTRPAALAYKPLEHQRVDIIAAYPGA
EEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCCCEEEEEECCCC
PRALVDASLASGAKALVIEGMGAGNVGGDIALAISDALDKKIPVVMSTRVDAGRVEGTYG
CHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEHHHCCCCCEEEEECCCCCCCCCCCC
GAGGGATLAAKGVIGADILRAGQSRILLACALATKTDPKTLF
CCCCCCEEEECCCHHHHHHHCCCCEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]