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The map label for this gene is phoB [H]

Identifier: 38232907

GI number: 38232907

Start: 254456

End: 255157

Strand: Reverse

Name: phoB [H]

Synonym: DIP0286

Alternate gene names: 38232907

Gene position: 255157-254456 (Counterclockwise)

Preceding gene: 38232908

Following gene: 38232906

Centisome position: 10.25

GC content: 55.56

Gene sequence:

>702_bases
ATGCATAGGCTTCGTGTTCTTGTTGTTGATGATGAGCCGCAGATGGTTCAGATTATTTCTTATGCTTTGGAGTTGGAGGG
CTGGGAGGTGTTGTCGGCTTCCTCAGCGCAGCGGGGGTGGCAGTTACTCAACGAGTATCGGTGTGATTTGGTGATTTTGG
ATGTGATGCTTCCTGACGCTTCGGGGTATGCGTTGTGTGAGCGGATTCGTGCTGCGGGGGATGTTGCTGGGACTCCGGTG
ATTATGTTGACTGCGCTTGGTGATACGGATAATCGGGTGGAGGGGCTTGAGGCGGGCGCGGATGATTATGTGGCTAAGCC
GTTTTCGCCGAAGGAGTTGGTGTTGCGGGCGCAGGCGGTGGTGCGTCGTAGTGGTGGGGTTGTGCAGCCGGAGTTGCGGG
AGATTATTGTGGGTGAGGTGTCGGTGAATCCGGCGACGCATGCGGTGGTGATTGGTGGTCGTAGGGTGGATACGACTGCT
ACGGAGGGGAAGCTGTTGCAGGCGTTGGTTTCACACCCGAATGAGGTGGTGAGTGTGAAGCGTTTGTTGAATGAGGTGTG
GGATACGACGGCGACTCAGGGTGGCCGAAATATGGTGAAGTCGACGGCGTATCGCTTGCGCAAGAAGTTAGAAAATGCGG
GTTTACCTGCTGATTCTATCCTTGCGGTCCGGGGTCAGGGGTACGTCTTTCAGTACGAGTGA

Upstream 100 bases:

>100_bases
ATTACGCAGAAGATTGTGCAGTTGCATCGTGGTTCGGTGTTGGTGGATTCTATTGCGGATCATGGAACGTCGGTGTTGAT
TACTCTTCCTAGGACTTACT

Downstream 100 bases:

>100_bases
TTCCCCTCGGTTGTGGTTGGTAACAGCACTGTTACCAATTGTTACCGCTATGGTCACTATGGTTACCGAGCGCGGAGCAC
AATCGCAATTAGTTGATAAA

Product: two-component regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MHRLRVLVVDDEPQMVQIISYALELEGWEVLSASSAQRGWQLLNEYRCDLVILDVMLPDASGYALCERIRAAGDVAGTPV
IMLTALGDTDNRVEGLEAGADDYVAKPFSPKELVLRAQAVVRRSGGVVQPELREIIVGEVSVNPATHAVVIGGRRVDTTA
TEGKLLQALVSHPNEVVSVKRLLNEVWDTTATQGGRNMVKSTAYRLRKKLENAGLPADSILAVRGQGYVFQYE

Sequences:

>Translated_233_residues
MHRLRVLVVDDEPQMVQIISYALELEGWEVLSASSAQRGWQLLNEYRCDLVILDVMLPDASGYALCERIRAAGDVAGTPV
IMLTALGDTDNRVEGLEAGADDYVAKPFSPKELVLRAQAVVRRSGGVVQPELREIIVGEVSVNPATHAVVIGGRRVDTTA
TEGKLLQALVSHPNEVVSVKRLLNEVWDTTATQGGRNMVKSTAYRLRKKLENAGLPADSILAVRGQGYVFQYE
>Mature_233_residues
MHRLRVLVVDDEPQMVQIISYALELEGWEVLSASSAQRGWQLLNEYRCDLVILDVMLPDASGYALCERIRAAGDVAGTPV
IMLTALGDTDNRVEGLEAGADDYVAKPFSPKELVLRAQAVVRRSGGVVQPELREIIVGEVSVNPATHAVVIGGRRVDTTA
TEGKLLQALVSHPNEVVSVKRLLNEVWDTTATQGGRNMVKSTAYRLRKKLENAGLPADSILAVRGQGYVFQYE

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=40.0881057268723, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1786784, Length=228, Percent_Identity=33.3333333333333, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1789809, Length=230, Percent_Identity=34.3478260869565, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1788394, Length=233, Percent_Identity=32.618025751073, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786911, Length=231, Percent_Identity=32.9004329004329, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787375, Length=228, Percent_Identity=32.8947368421053, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1790860, Length=231, Percent_Identity=34.1991341991342, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI2367329, Length=231, Percent_Identity=33.7662337662338, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1790863, Length=239, Percent_Identity=29.7071129707113, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1790552, Length=229, Percent_Identity=31.8777292576419, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI87082012, Length=227, Percent_Identity=29.0748898678414, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789402, Length=230, Percent_Identity=31.7391304347826, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=28.82096069869, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=35.0877192982456, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693140, Length=237, Percent_Identity=25.7383966244726, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1789149, Length=138, Percent_Identity=31.8840579710145, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25451; Mature: 25451

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRLRVLVVDDEPQMVQIISYALELEGWEVLSASSAQRGWQLLNEYRCDLVILDVMLPDA
CCEEEEEEECCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCEEEEEEECCCC
SGYALCERIRAAGDVAGTPVIMLTALGDTDNRVEGLEAGADDYVAKPFSPKELVLRAQAV
CCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
VRRSGGVVQPELREIIVGEVSVNPATHAVVIGGRRVDTTATEGKLLQALVSHPNEVVSVK
HHHCCCCCCHHHHHHEEECEECCCCCEEEEECCEEECCCCHHHHHHHHHHHCCHHHHHHH
RLLNEVWDTTATQGGRNMVKSTAYRLRKKLENAGLPADSILAVRGQGYVFQYE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCEEEEEC
>Mature Secondary Structure
MHRLRVLVVDDEPQMVQIISYALELEGWEVLSASSAQRGWQLLNEYRCDLVILDVMLPDA
CCEEEEEEECCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCEEEEEEECCCC
SGYALCERIRAAGDVAGTPVIMLTALGDTDNRVEGLEAGADDYVAKPFSPKELVLRAQAV
CCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
VRRSGGVVQPELREIIVGEVSVNPATHAVVIGGRRVDTTATEGKLLQALVSHPNEVVSVK
HHHCCCCCCHHHHHHEEECEECCCCCEEEEECCEEECCCCHHHHHHHHHHHCCHHHHHHH
RLLNEVWDTTATQGGRNMVKSTAYRLRKKLENAGLPADSILAVRGQGYVFQYE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]