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The map label for this gene is leuA [H]

Identifier: 161598679

GI number: 161598679

Start: 228555

End: 230267

Strand: Reverse

Name: leuA [H]

Synonym: DIP0266

Alternate gene names: 161598679

Gene position: 230267-228555 (Counterclockwise)

Preceding gene: 38232897

Following gene: 38232888

Centisome position: 9.25

GC content: 55.58

Gene sequence:

>1713_bases
ATGGCAGTAAATCGATACCAGCCGTTTCACGTCGAAGTAGAAAACATCGATCTCCCCGACCGCACTTGGCCGCAAAAACG
CATCACTAAGGCGCCACAGTGGTGCGCAGTAGACCTTCGTGATGGCAACCAAGCACTCATCGACCCAATGAGCCCTGAGC
GCAAGCGCCGCATGTTTAACCTGCTGGTCAACATGGGTTACAAGGAAATCGAAGTCGGCTTCCCATCCGCCTCGCAAACC
GACTTCGACTTTGTTCGCGAAATCATTGAGAACAATATGATTCCGAACGATGTCACCATCCAGGTCCTCGTACAAGCACG
TGAACACCTGATCCGTCGCACATTCGAAGCATGCGAGGGCGCGAAAAACGTGATCGTGCACTTCTACAACTCCACATCGA
TCCTGCAGCGAGACGTAGTATTCCGCAAAGACAAAGCAGCAATTAAAAAGTTGGCTACCGATGCTGCCGAGCTGATCAAA
TCGATCGCAGTGGACTACCCCGATACCAATTGGCGCTGGGAGTACTCCCCCGAGTCCTACACCGGAACCGAATTGACATA
CGCCAAGGAAGTCGTCGATGCCGTCGTCGCGGTCATGGACCCAACCCCAGAAAACCCTATCATCATCAACCTGCCTTCAA
CAGTTGAGATGATCACCCCAAACGTTTACGCCGATGGCATTGAATGGATGCACCGCAACCTCAACCGACGCGACAGCATC
ATCTTGTCTCTGCACCCCCACAACGATCGCGGCACCGGTGTCGCAGCAGCAGAACTGGGCTACCTCGCTGGCGCGGACCG
AATCGAGGGCTGCCTGTTCGGCAATGGCGAACGCACCGGCAACGTCTGCTTGGTCACCCTTGGCCTGAACATGTTCACCC
AAGGCGTGGACCCACAAATTGATTTCTCGGACATCGATCAAATCCGTCGCACCGTTGAATACTGCAACCAGCTGCGCGTC
CCAGAACGCCACCCCTATGGTGGCGACCTAGTATTCACAGCATTCTCTGGCTCCCACCAAGATGCCGTTAACAAGGGCCT
GGATGCCATGGCATCCCGAGTACACCCCGGCGCGACCCACACCGAAGTCTCCTGGGACGAGCTGCGTGGAGAAACTTGGG
AGGTTCCATACCTGCCGATCGACCCGAAGGATGTGGGCCGCAACTACGAGGCAGTGATCCGTGTGAACTCGCAGTCCGGC
AAGGGTGGTGTTGCTTACATTATGAAGACCGATCACGGTTTGGCGCTGCCTCGTCCTATGCAGGTCGAGTTTTCTTCGAT
CGTGCAGGAAGTCACCGATGCCGAGGGCGGCGAGGTGAATTCCAAGAACATGTGGGACATTTTCGCCCGCGAATACTTGG
ATAGCACCAGCCCTGTGGAGCAAGTCTCTATGAGCGTTAATGCTGCGGAAACTGAAAACGATGAGGCTCGCATTCACGCC
CGCCTGATCGTTAATGGTTCTGAGACGACTATCGAAGGCCACGGTAACGGCCCAATCGCCGCATATGCCAACGCGCTGGA
GTCCTTGGGCATTGATGTTGAGGTGCAGGAATATGAGCAGCATGCCCGTACCGCAGGTGACGATGCCGAAGCTGCCGCCT
ACATTTTGGCGGAGGTTAACGGCAAGAAGTTCTGGGGCGTGGGCATCGCAGGTTCCATCACCTACGCTTCCCTGAAGGCA
ATTACTTCTGCGGTCAACCGTGCGCTGAGTTAA

Upstream 100 bases:

>100_bases
CTCCCCAGATACTTTCATCTCCGCCCCTGCAGCCATTGTCACCCCACGTGGTGATAAAGCACCAGGTCAGGCTCCATGGA
ACACTCAGCGCAATTCCTCC

Downstream 100 bases:

>100_bases
ATAACTACTTACACTTGGCGGACATGAAAGCAGGCACTAAAGCAGGCATGAAGGCAAGCATGAACGAGACGATTCGCACC
CAGCTTAACCACCGCACTAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MAVNRYQPFHVEVENIDLPDRTWPQKRITKAPQWCAVDLRDGNQALIDPMSPERKRRMFNLLVNMGYKEIEVGFPSASQT
DFDFVREIIENNMIPNDVTIQVLVQAREHLIRRTFEACEGAKNVIVHFYNSTSILQRDVVFRKDKAAIKKLATDAAELIK
SIAVDYPDTNWRWEYSPESYTGTELTYAKEVVDAVVAVMDPTPENPIIINLPSTVEMITPNVYADGIEWMHRNLNRRDSI
ILSLHPHNDRGTGVAAAELGYLAGADRIEGCLFGNGERTGNVCLVTLGLNMFTQGVDPQIDFSDIDQIRRTVEYCNQLRV
PERHPYGGDLVFTAFSGSHQDAVNKGLDAMASRVHPGATHTEVSWDELRGETWEVPYLPIDPKDVGRNYEAVIRVNSQSG
KGGVAYIMKTDHGLALPRPMQVEFSSIVQEVTDAEGGEVNSKNMWDIFAREYLDSTSPVEQVSMSVNAAETENDEARIHA
RLIVNGSETTIEGHGNGPIAAYANALESLGIDVEVQEYEQHARTAGDDAEAAAYILAEVNGKKFWGVGIAGSITYASLKA
ITSAVNRALS

Sequences:

>Translated_570_residues
MAVNRYQPFHVEVENIDLPDRTWPQKRITKAPQWCAVDLRDGNQALIDPMSPERKRRMFNLLVNMGYKEIEVGFPSASQT
DFDFVREIIENNMIPNDVTIQVLVQAREHLIRRTFEACEGAKNVIVHFYNSTSILQRDVVFRKDKAAIKKLATDAAELIK
SIAVDYPDTNWRWEYSPESYTGTELTYAKEVVDAVVAVMDPTPENPIIINLPSTVEMITPNVYADGIEWMHRNLNRRDSI
ILSLHPHNDRGTGVAAAELGYLAGADRIEGCLFGNGERTGNVCLVTLGLNMFTQGVDPQIDFSDIDQIRRTVEYCNQLRV
PERHPYGGDLVFTAFSGSHQDAVNKGLDAMASRVHPGATHTEVSWDELRGETWEVPYLPIDPKDVGRNYEAVIRVNSQSG
KGGVAYIMKTDHGLALPRPMQVEFSSIVQEVTDAEGGEVNSKNMWDIFAREYLDSTSPVEQVSMSVNAAETENDEARIHA
RLIVNGSETTIEGHGNGPIAAYANALESLGIDVEVQEYEQHARTAGDDAEAAAYILAEVNGKKFWGVGIAGSITYASLKA
ITSAVNRALS
>Mature_569_residues
AVNRYQPFHVEVENIDLPDRTWPQKRITKAPQWCAVDLRDGNQALIDPMSPERKRRMFNLLVNMGYKEIEVGFPSASQTD
FDFVREIIENNMIPNDVTIQVLVQAREHLIRRTFEACEGAKNVIVHFYNSTSILQRDVVFRKDKAAIKKLATDAAELIKS
IAVDYPDTNWRWEYSPESYTGTELTYAKEVVDAVVAVMDPTPENPIIINLPSTVEMITPNVYADGIEWMHRNLNRRDSII
LSLHPHNDRGTGVAAAELGYLAGADRIEGCLFGNGERTGNVCLVTLGLNMFTQGVDPQIDFSDIDQIRRTVEYCNQLRVP
ERHPYGGDLVFTAFSGSHQDAVNKGLDAMASRVHPGATHTEVSWDELRGETWEVPYLPIDPKDVGRNYEAVIRVNSQSGK
GGVAYIMKTDHGLALPRPMQVEFSSIVQEVTDAEGGEVNSKNMWDIFAREYLDSTSPVEQVSMSVNAAETENDEARIHAR
LIVNGSETTIEGHGNGPIAAYANALESLGIDVEVQEYEQHARTAGDDAEAAAYILAEVNGKKFWGVGIAGSITYASLKAI
TSAVNRALS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=552, Percent_Identity=25.3623188405797, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324225, Length=574, Percent_Identity=47.0383275261324, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6324682, Length=559, Percent_Identity=47.2271914132379, Blast_Score=520, Evalue=1e-148,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 63316; Mature: 63185

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVNRYQPFHVEVENIDLPDRTWPQKRITKAPQWCAVDLRDGNQALIDPMSPERKRRMFN
CCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHH
LLVNMGYKEIEVGFPSASQTDFDFVREIIENNMIPNDVTIQVLVQAREHLIRRTFEACEG
HHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
AKNVIVHFYNSTSILQRDVVFRKDKAAIKKLATDAAELIKSIAVDYPDTNWRWEYSPESY
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
TGTELTYAKEVVDAVVAVMDPTPENPIIINLPSTVEMITPNVYADGIEWMHRNLNRRDSI
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCCE
ILSLHPHNDRGTGVAAAELGYLAGADRIEGCLFGNGERTGNVCLVTLGLNMFTQGVDPQI
EEEEECCCCCCCCEEHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCC
DFSDIDQIRRTVEYCNQLRVPERHPYGGDLVFTAFSGSHQDAVNKGLDAMASRVHPGATH
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
TEVSWDELRGETWEVPYLPIDPKDVGRNYEAVIRVNSQSGKGGVAYIMKTDHGLALPRPM
CCCCHHHHCCCEECCCEECCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCC
QVEFSSIVQEVTDAEGGEVNSKNMWDIFAREYLDSTSPVEQVSMSVNAAETENDEARIHA
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHEECCCCCCCCCCEEEEE
RLIVNGSETTIEGHGNGPIAAYANALESLGIDVEVQEYEQHARTAGDDAEAAAYILAEVN
EEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHEEEEEECC
GKKFWGVGIAGSITYASLKAITSAVNRALS
CCEEEEECCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVNRYQPFHVEVENIDLPDRTWPQKRITKAPQWCAVDLRDGNQALIDPMSPERKRRMFN
CCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHH
LLVNMGYKEIEVGFPSASQTDFDFVREIIENNMIPNDVTIQVLVQAREHLIRRTFEACEG
HHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
AKNVIVHFYNSTSILQRDVVFRKDKAAIKKLATDAAELIKSIAVDYPDTNWRWEYSPESY
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
TGTELTYAKEVVDAVVAVMDPTPENPIIINLPSTVEMITPNVYADGIEWMHRNLNRRDSI
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCCE
ILSLHPHNDRGTGVAAAELGYLAGADRIEGCLFGNGERTGNVCLVTLGLNMFTQGVDPQI
EEEEECCCCCCCCEEHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCC
DFSDIDQIRRTVEYCNQLRVPERHPYGGDLVFTAFSGSHQDAVNKGLDAMASRVHPGATH
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
TEVSWDELRGETWEVPYLPIDPKDVGRNYEAVIRVNSQSGKGGVAYIMKTDHGLALPRPM
CCCCHHHHCCCEECCCEECCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCC
QVEFSSIVQEVTDAEGGEVNSKNMWDIFAREYLDSTSPVEQVSMSVNAAETENDEARIHA
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHEECCCCCCCCCCEEEEE
RLIVNGSETTIEGHGNGPIAAYANALESLGIDVEVQEYEQHARTAGDDAEAAAYILAEVN
EEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHEEEEEECC
GKKFWGVGIAGSITYASLKAITSAVNRALS
CCEEEEECCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA