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The map label for this gene is gidA

Identifier: 21675089

GI number: 21675089

Start: 2146558

End: 2148423

Strand: Direct

Name: gidA

Synonym: CT2283

Alternate gene names: 21675089

Gene position: 2146558-2148423 (Clockwise)

Preceding gene: 21675087

Following gene: 21675090

Centisome position: 99.61

GC content: 51.02

Gene sequence:

>1866_bases
ATGTATGATGTCATAGTTGTCGGGGCTGGCCATGCCGGCTGCGAAGCCGCACTCGCCGTCGCACGTGGCGGCTTGCACTG
TCTTCTCATTACCTCTGACCTCTCGGCGGTTGCCCGCATGTCCTGCAACCCGGCGATAGGTGGCGTTGCCAAAGGCCAAA
TAACAAGGGAAATCGACGCGCTTGGCGGCGAAATGGGCAAAGCTATCGATGCCACAGGCATCCAGTTCAGAATGCTCAAC
CGCAGCAAAGGCCCAGCAATGCACTCACCGCGCGCACAGGCAGACAAGACACAGTACAGCCTTTACATGAGAAGGATTGT
CGAGCATGAACCAAACATCGACCTGCTGCAGGATACGGTTATTGGAGTTTCGGCAAACTCTGGCAAGTTCAGTTCTGTAA
CAGTGCGATCAGGCCGAGCAATCCAGGCCAAAGCCGCTATTCTTGCCTGCGGCACCTTTCTGAACGGCCTGATTCATATC
GGCATGGACCACTTCCCGGGGGGACGAAGCACAGCAGAACCGCCCGTTGAAGGCCTAACCGAATCTCTCGCTTCGCTCGG
ATTTTCTTTCGGGAGACTCAAAACTGGCACGCCACCGCGAATCGACTCAAGAAGTGTCGATTACACCATTGTCACTGAGC
AACCTGGAGATGTCGATCCGGTACCATTTTCTTTTTCTTCAACAAGCGTTGCTAACCGAAACCTTGTCAGTTGCTATCTG
ACCAAAACAACGGAAAAAACGCACGACATTCTGCGAACCGGCTTTGACCGATCTCCGCTCTTTACCGGCAAGGTTCAAGG
TGTTGGGCCAAGGTATTGTCCGTCAATTGAAGACAAAATTTCCCGTTTTCCAGACAAATCGAGCCATCATATTTTTCTGG
AACCAGAAGGTACTGATACTGTAGAAATGTATGTCAACGGGTTTTCGACATCTCTGCCCGAAGATATTCAGATCGCAGGA
CTGCGATCGATCCCCGGACTTGAAGAAGCCAAAATGATTCGCCCCGGTTATGCCATCGAGTATGACTTTTTCCATCCCTG
GCAAATCAGGTCTACAATGGAGACCCGACCCGTTGAAAACCTCTTCTTTGCCGGCCAGATTAACGGTACATCCGGCTATG
AAGAGGCTGCTGCACAGGGCCTAATGGCCGGTATAAATGCGGTTCGCAAAATTCTTGGCAAAGAGCTGATCGTCTTGGGA
CGTGATCAGGCATATATCGGTGTACTTATCGATGACCTGATAACCAAGGAGACCAAGGAGCCTTATCGCATGTTCACCTC
ATCAGCAGAACATCGACTGATCCTGCGACATGATAATGCCGATCTACGACTGCGGAAAATTGGATACGATTGCAATCTGG
TCTCCTCAGACGATCTACATCGAACGGAATCAATTATCAAGCGCGTTCAACACTGTCTTGAAGTAATGAAAACGGCCAAA
GTAACGCCAGCGGAGATCAACACACTGCTGATGAACAAGGGCCTACAGGAACTCAAAACCCCTGCCCGCGCGTTGAGTTT
GATCAAACGCCCCGGCATTAGCCTCCAGGATATTCTGGAGCACTCGCTCTCCGTTCGATCCGCTGCGGAAGAGTTATGCA
ATGACCCAAGGGTTGCCGAACAGGTGCAGATCGAGATAAAGTACGAAGGCTATATCAAGCGGGAACAACTTGTAGCCGAC
CGTATAGCTCGTCTTGATTCCTTGCATATTCCGGATAATTTCAATTATGATTCACTGAATTCCCTTTCAAGCGAGGGAAG
GGAAAAACTACTGAAGCACCGTCCTGCGACCATCGGTCAAGCCTCTAGAATTCTCGGGGTCTCTCCGTCCGACGTCTCCA
TACTCATGATTCGGCTTGGCCGGTAA

Upstream 100 bases:

>100_bases
AATTACAGTTAGATAGAAAACTATTATCTGGGCGACCCGGATTTCAATAGTTATTTCAACAACCATATGTAGATGTTTCA
CGTGAAACAACTCTTTTTTC

Downstream 100 bases:

>100_bases
CGCTGTTTCACGTGAAACATGTATTCAATCTTGCTCTCTGGTACCATGAACAACATTGTCAATTTTTCTTTCGAGAATGA
ACTGCTTTTCGGTAAAGACC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MYDVIVVGAGHAGCEAALAVARGGLHCLLITSDLSAVARMSCNPAIGGVAKGQITREIDALGGEMGKAIDATGIQFRMLN
RSKGPAMHSPRAQADKTQYSLYMRRIVEHEPNIDLLQDTVIGVSANSGKFSSVTVRSGRAIQAKAAILACGTFLNGLIHI
GMDHFPGGRSTAEPPVEGLTESLASLGFSFGRLKTGTPPRIDSRSVDYTIVTEQPGDVDPVPFSFSSTSVANRNLVSCYL
TKTTEKTHDILRTGFDRSPLFTGKVQGVGPRYCPSIEDKISRFPDKSSHHIFLEPEGTDTVEMYVNGFSTSLPEDIQIAG
LRSIPGLEEAKMIRPGYAIEYDFFHPWQIRSTMETRPVENLFFAGQINGTSGYEEAAAQGLMAGINAVRKILGKELIVLG
RDQAYIGVLIDDLITKETKEPYRMFTSSAEHRLILRHDNADLRLRKIGYDCNLVSSDDLHRTESIIKRVQHCLEVMKTAK
VTPAEINTLLMNKGLQELKTPARALSLIKRPGISLQDILEHSLSVRSAAEELCNDPRVAEQVQIEIKYEGYIKREQLVAD
RIARLDSLHIPDNFNYDSLNSLSSEGREKLLKHRPATIGQASRILGVSPSDVSILMIRLGR

Sequences:

>Translated_621_residues
MYDVIVVGAGHAGCEAALAVARGGLHCLLITSDLSAVARMSCNPAIGGVAKGQITREIDALGGEMGKAIDATGIQFRMLN
RSKGPAMHSPRAQADKTQYSLYMRRIVEHEPNIDLLQDTVIGVSANSGKFSSVTVRSGRAIQAKAAILACGTFLNGLIHI
GMDHFPGGRSTAEPPVEGLTESLASLGFSFGRLKTGTPPRIDSRSVDYTIVTEQPGDVDPVPFSFSSTSVANRNLVSCYL
TKTTEKTHDILRTGFDRSPLFTGKVQGVGPRYCPSIEDKISRFPDKSSHHIFLEPEGTDTVEMYVNGFSTSLPEDIQIAG
LRSIPGLEEAKMIRPGYAIEYDFFHPWQIRSTMETRPVENLFFAGQINGTSGYEEAAAQGLMAGINAVRKILGKELIVLG
RDQAYIGVLIDDLITKETKEPYRMFTSSAEHRLILRHDNADLRLRKIGYDCNLVSSDDLHRTESIIKRVQHCLEVMKTAK
VTPAEINTLLMNKGLQELKTPARALSLIKRPGISLQDILEHSLSVRSAAEELCNDPRVAEQVQIEIKYEGYIKREQLVAD
RIARLDSLHIPDNFNYDSLNSLSSEGREKLLKHRPATIGQASRILGVSPSDVSILMIRLGR
>Mature_621_residues
MYDVIVVGAGHAGCEAALAVARGGLHCLLITSDLSAVARMSCNPAIGGVAKGQITREIDALGGEMGKAIDATGIQFRMLN
RSKGPAMHSPRAQADKTQYSLYMRRIVEHEPNIDLLQDTVIGVSANSGKFSSVTVRSGRAIQAKAAILACGTFLNGLIHI
GMDHFPGGRSTAEPPVEGLTESLASLGFSFGRLKTGTPPRIDSRSVDYTIVTEQPGDVDPVPFSFSSTSVANRNLVSCYL
TKTTEKTHDILRTGFDRSPLFTGKVQGVGPRYCPSIEDKISRFPDKSSHHIFLEPEGTDTVEMYVNGFSTSLPEDIQIAG
LRSIPGLEEAKMIRPGYAIEYDFFHPWQIRSTMETRPVENLFFAGQINGTSGYEEAAAQGLMAGINAVRKILGKELIVLG
RDQAYIGVLIDDLITKETKEPYRMFTSSAEHRLILRHDNADLRLRKIGYDCNLVSSDDLHRTESIIKRVQHCLEVMKTAK
VTPAEINTLLMNKGLQELKTPARALSLIKRPGISLQDILEHSLSVRSAAEELCNDPRVAEQVQIEIKYEGYIKREQLVAD
RIARLDSLHIPDNFNYDSLNSLSSEGREKLLKHRPATIGQASRILGVSPSDVSILMIRLGR

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=629, Percent_Identity=39.4276629570747, Blast_Score=421, Evalue=1e-117,
Organism=Homo sapiens, GI19882217, Length=654, Percent_Identity=38.0733944954128, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI183227703, Length=669, Percent_Identity=37.219730941704, Blast_Score=400, Evalue=1e-111,
Organism=Escherichia coli, GI2367273, Length=626, Percent_Identity=47.7635782747604, Blast_Score=588, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI17534255, Length=622, Percent_Identity=37.2990353697749, Blast_Score=402, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321202, Length=628, Percent_Identity=41.5605095541401, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24658174, Length=625, Percent_Identity=40.64, Blast_Score=422, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_CHLTE (Q8KA85)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_663154.1
- PDB:   3CP8
- PDBsum:   3CP8
- ProteinModelPortal:   Q8KA85
- SMR:   Q8KA85
- GeneID:   1007276
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT2283
- NMPDR:   fig|194439.1.peg.2248
- TIGR:   CT2283
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   CTEP194439:CT_2283-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 68098; Mature: 68098

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 120-120 BINDING 366-366

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDVIVVGAGHAGCEAALAVARGGLHCLLITSDLSAVARMSCNPAIGGVAKGQITREIDA
CEEEEEEECCCCCHHHHHHHHCCCEEEEEEECCHHHHHHHCCCCCCCCCCCCHHHHHHHH
LGGEMGKAIDATGIQFRMLNRSKGPAMHSPRAQADKTQYSLYMRRIVEHEPNIDLLQDTV
HCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHEE
IGVSANSGKFSSVTVRSGRAIQAKAAILACGTFLNGLIHIGMDHFPGGRSTAEPPVEGLT
EEEECCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
ESLASLGFSFGRLKTGTPPRIDSRSVDYTIVTEQPGDVDPVPFSFSSTSVANRNLVSCYL
HHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEE
TKTTEKTHDILRTGFDRSPLFTGKVQGVGPRYCPSIEDKISRFPDKSSHHIFLEPEGTDT
ECCCHHHHHHHHCCCCCCCEEECEECCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCE
VEMYVNGFSTSLPEDIQIAGLRSIPGLEEAKMIRPGYAIEYDFFHPWQIRSTMETRPVEN
EEEEEECCCCCCCCCEEEECCCCCCCCCHHHEECCCEEEEECCCCCHHHCCHHHCCCCCC
LFFAGQINGTSGYEEAAAQGLMAGINAVRKILGKELIVLGRDQAYIGVLIDDLITKETKE
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEHHHHHHHHHHCCCC
PYRMFTSSAEHRLILRHDNADLRLRKIGYDCNLVSSDDLHRTESIIKRVQHCLEVMKTAK
HHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
VTPAEINTLLMNKGLQELKTPARALSLIKRPGISLQDILEHSLSVRSAAEELCNDPRVAE
CCHHHHHHHHHHCCHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
QVQIEIKYEGYIKREQLVADRIARLDSLHIPDNFNYDSLNSLSSEGREKLLKHRPATIGQ
EEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
ASRILGVSPSDVSILMIRLGR
HHHEECCCCCCCEEEEEEECC
>Mature Secondary Structure
MYDVIVVGAGHAGCEAALAVARGGLHCLLITSDLSAVARMSCNPAIGGVAKGQITREIDA
CEEEEEEECCCCCHHHHHHHHCCCEEEEEEECCHHHHHHHCCCCCCCCCCCCHHHHHHHH
LGGEMGKAIDATGIQFRMLNRSKGPAMHSPRAQADKTQYSLYMRRIVEHEPNIDLLQDTV
HCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHEE
IGVSANSGKFSSVTVRSGRAIQAKAAILACGTFLNGLIHIGMDHFPGGRSTAEPPVEGLT
EEEECCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
ESLASLGFSFGRLKTGTPPRIDSRSVDYTIVTEQPGDVDPVPFSFSSTSVANRNLVSCYL
HHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEE
TKTTEKTHDILRTGFDRSPLFTGKVQGVGPRYCPSIEDKISRFPDKSSHHIFLEPEGTDT
ECCCHHHHHHHHCCCCCCCEEECEECCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCE
VEMYVNGFSTSLPEDIQIAGLRSIPGLEEAKMIRPGYAIEYDFFHPWQIRSTMETRPVEN
EEEEEECCCCCCCCCEEEECCCCCCCCCHHHEECCCEEEEECCCCCHHHCCHHHCCCCCC
LFFAGQINGTSGYEEAAAQGLMAGINAVRKILGKELIVLGRDQAYIGVLIDDLITKETKE
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEHHHHHHHHHHCCCC
PYRMFTSSAEHRLILRHDNADLRLRKIGYDCNLVSSDDLHRTESIIKRVQHCLEVMKTAK
HHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
VTPAEINTLLMNKGLQELKTPARALSLIKRPGISLQDILEHSLSVRSAAEELCNDPRVAE
CCHHHHHHHHHHCCHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
QVQIEIKYEGYIKREQLVADRIARLDSLHIPDNFNYDSLNSLSSEGREKLLKHRPATIGQ
EEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
ASRILGVSPSDVSILMIRLGR
HHHEECCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901