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The map label for this gene is clpB-1

Identifier: 21675087

GI number: 21675087

Start: 2144639

End: 2145967

Strand: Direct

Name: clpB-1

Synonym: CT2281

Alternate gene names: 21675087

Gene position: 2144639-2145967 (Clockwise)

Preceding gene: 21675086

Following gene: 21675089

Centisome position: 99.52

GC content: 56.36

Gene sequence:

>1329_bases
ATGCATTTCGATCCGAACAAATTTACCGTCAAGGCCCAGGAAGCCCTTCAGGCAGCGTCAATGCTGGCAAGCAGCAAGCA
AAACCAGCAGATTGAACCCGAGCACCTGCTGAGCGTTATGCTTGGCGATCATGACAATATTGCCTGCCAGATAGCGCGAA
AACTCGAAACGCCCGTCGATACGCTCCTCAGCGTCGTCGATCGGGAAATCGACCGAATTCCCAAAGTGACCGGAGCGTCA
GCAACCGGTCAATATATCTCCAGCGACCTCGGTAAGGTGTTCGACACCGCCCTCAAGGAGGCAGAGCAGCTCAAAGACGA
GTATATAAGCTCCGAGCATCTTTTCATCGCTATGAGCGAAGCAGGAGTCAAAGTTTCGAAACTCCTGAAAGACGCAGGAA
TCGACCGCAACGCCATACTCAAGGTGCTCACCTCGTTCCGGGGCTCCCAGCGCGTAACCAGCCAGAATGCGGAGGAGAGC
TATCAGTCACTGAAAAAATATTCGCGAAACCTGAACGATCTGGTCATAAAGGGGAAACTCGATCCGGTGATTGGCCGCGA
CGACGAAATCCGCCGCGTGCTCCAGATTCTGAGCCGCCGAACCAAAAATAATCCGGTACTCATCGGCGAGCCGGGCGTCG
GCAAAACGGCCATCGTCGAAGGCATCGCCCAGCGTATCGTGGGAGGCGACGTACCGGAGAACCTCAAGTCGAAGCAGATC
GCCGCACTCGACATCGCGGCACTGGTGGCAGGAACCAAGTTCAGAGGAGAATTTGAGGAGCGACTGAAGGCGCTGGTCAA
GGAGGTTCAGGCCTCCGATGGCGAGGTAATTCTTTTCATCGACGAGCTGCATCTCCTGGTCGGAGCGGGATCGGCGGAAG
GTTCGATGGACGCGGCCAACATCCTCAAGCCAGCGCTGGCAAGAGGTGAATTGCGCTGTATCGGAGCAACCACACTCGAC
GAGTACCGCAAGCACATTGAAAAGGACGCAGCGCTTGAACGGCGCTTCCAGACAGTCATAGTCGATCAGCCCAGTGTCGA
GGATACCGTCTCGATCCTGCGCGGACTGAAAGAGAAATACGAAATCCACCACGGAGTGCGCATCAAGGATGCCGCCCTGG
TGGCGGCCGCAGAGCTTTCGAATCGCTACATTGCAGACCGCTTCCTGCCGGACAAGGCCATCGATCTGATTGATGAAGCC
TGCTCGCGCCTGCGGCTTGAAATCGACAGCGAACCCGAAGAACTTGACCGAATTAACCGTGAGCTTCGCCGGCTTGAAAT
CGAGCGTGAAGCCCTCAAGCGCGAACTCGAAGCGACCGGCAGCGTCTGA

Upstream 100 bases:

>100_bases
TTTTTTGGCGGTTGTTATGATGATAAGGCGCGGCAGGCCCAGCTCTTGAGCTTGCCGCGACCATGAAAAACATACCGAAA
ACAGCAAGAAACGATCAACT

Downstream 100 bases:

>100_bases
CGCCTCCATATGGTGCGATGCAATACAATACCATGAGGCGGCTTAGCCCCCTTGCAGCCGCCTCCGCTTTTAGAAAGCTG
TCCATCGGTTATTCTACATT

Product: ATP-dependent Clp protease, ATP-binding subunit ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MHFDPNKFTVKAQEALQAASMLASSKQNQQIEPEHLLSVMLGDHDNIACQIARKLETPVDTLLSVVDREIDRIPKVTGAS
ATGQYISSDLGKVFDTALKEAEQLKDEYISSEHLFIAMSEAGVKVSKLLKDAGIDRNAILKVLTSFRGSQRVTSQNAEES
YQSLKKYSRNLNDLVIKGKLDPVIGRDDEIRRVLQILSRRTKNNPVLIGEPGVGKTAIVEGIAQRIVGGDVPENLKSKQI
AALDIAALVAGTKFRGEFEERLKALVKEVQASDGEVILFIDELHLLVGAGSAEGSMDAANILKPALARGELRCIGATTLD
EYRKHIEKDAALERRFQTVIVDQPSVEDTVSILRGLKEKYEIHHGVRIKDAALVAAAELSNRYIADRFLPDKAIDLIDEA
CSRLRLEIDSEPEELDRINRELRRLEIEREALKRELEATGSV

Sequences:

>Translated_442_residues
MHFDPNKFTVKAQEALQAASMLASSKQNQQIEPEHLLSVMLGDHDNIACQIARKLETPVDTLLSVVDREIDRIPKVTGAS
ATGQYISSDLGKVFDTALKEAEQLKDEYISSEHLFIAMSEAGVKVSKLLKDAGIDRNAILKVLTSFRGSQRVTSQNAEES
YQSLKKYSRNLNDLVIKGKLDPVIGRDDEIRRVLQILSRRTKNNPVLIGEPGVGKTAIVEGIAQRIVGGDVPENLKSKQI
AALDIAALVAGTKFRGEFEERLKALVKEVQASDGEVILFIDELHLLVGAGSAEGSMDAANILKPALARGELRCIGATTLD
EYRKHIEKDAALERRFQTVIVDQPSVEDTVSILRGLKEKYEIHHGVRIKDAALVAAAELSNRYIADRFLPDKAIDLIDEA
CSRLRLEIDSEPEELDRINRELRRLEIEREALKRELEATGSV
>Mature_442_residues
MHFDPNKFTVKAQEALQAASMLASSKQNQQIEPEHLLSVMLGDHDNIACQIARKLETPVDTLLSVVDREIDRIPKVTGAS
ATGQYISSDLGKVFDTALKEAEQLKDEYISSEHLFIAMSEAGVKVSKLLKDAGIDRNAILKVLTSFRGSQRVTSQNAEES
YQSLKKYSRNLNDLVIKGKLDPVIGRDDEIRRVLQILSRRTKNNPVLIGEPGVGKTAIVEGIAQRIVGGDVPENLKSKQI
AALDIAALVAGTKFRGEFEERLKALVKEVQASDGEVILFIDELHLLVGAGSAEGSMDAANILKPALARGELRCIGATTLD
EYRKHIEKDAALERRFQTVIVDQPSVEDTVSILRGLKEKYEIHHGVRIKDAALVAAAELSNRYIADRFLPDKAIDLIDEA
CSRLRLEIDSEPEELDRINRELRRLEIEREALKRELEATGSV

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family

Homologues:

Organism=Escherichia coli, GI1788943, Length=430, Percent_Identity=54.8837209302326, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1787109, Length=255, Percent_Identity=53.7254901960784, Blast_Score=274, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6320464, Length=280, Percent_Identity=62.1428571428571, Blast_Score=368, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6323002, Length=447, Percent_Identity=44.5190156599553, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CLPB2_CHLTE (Q8KA87)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_663152.1
- ProteinModelPortal:   Q8KA87
- GeneID:   1007278
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT2281
- NMPDR:   fig|194439.1.peg.2246
- TIGR:   CT2281
- HOGENOM:   HBG413133
- OMA:   AGDVPEN
- ProtClustDB:   CLSK638152
- BioCyc:   CTEP194439:CT_2281-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR004176
- InterPro:   IPR023150
- Gene3D:   G3DSA:1.10.1780.10
- SMART:   SM00382

Pfam domain/function: PF00004 AAA; PF02861 Clp_N

EC number: NA

Molecular weight: Translated: 48930; Mature: 48930

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00870 CLPAB_1; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFDPNKFTVKAQEALQAASMLASSKQNQQIEPEHLLSVMLGDHDNIACQIARKLETPVD
CCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHH
TLLSVVDREIDRIPKVTGASATGQYISSDLGKVFDTALKEAEQLKDEYISSEHLFIAMSE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
AGVKVSKLLKDAGIDRNAILKVLTSFRGSQRVTSQNAEESYQSLKKYSRNLNDLVIKGKL
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCHHEEEECCC
DPVIGRDDEIRRVLQILSRRTKNNPVLIGEPGVGKTAIVEGIAQRIVGGDVPENLKSKQI
CCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
AALDIAALVAGTKFRGEFEERLKALVKEVQASDGEVILFIDELHLLVGAGSAEGSMDAAN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCCCCCCCHHH
ILKPALARGELRCIGATTLDEYRKHIEKDAALERRFQTVIVDQPSVEDTVSILRGLKEKY
HHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHH
EIHHGVRIKDAALVAAAELSNRYIADRFLPDKAIDLIDEACSRLRLEIDSEPEELDRINR
HHHCCCEEHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHH
ELRRLEIEREALKRELEATGSV
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHFDPNKFTVKAQEALQAASMLASSKQNQQIEPEHLLSVMLGDHDNIACQIARKLETPVD
CCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHH
TLLSVVDREIDRIPKVTGASATGQYISSDLGKVFDTALKEAEQLKDEYISSEHLFIAMSE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
AGVKVSKLLKDAGIDRNAILKVLTSFRGSQRVTSQNAEESYQSLKKYSRNLNDLVIKGKL
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCHHEEEECCC
DPVIGRDDEIRRVLQILSRRTKNNPVLIGEPGVGKTAIVEGIAQRIVGGDVPENLKSKQI
CCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
AALDIAALVAGTKFRGEFEERLKALVKEVQASDGEVILFIDELHLLVGAGSAEGSMDAAN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCCCCCCCHHH
ILKPALARGELRCIGATTLDEYRKHIEKDAALERRFQTVIVDQPSVEDTVSILRGLKEKY
HHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHH
EIHHGVRIKDAALVAAAELSNRYIADRFLPDKAIDLIDEACSRLRLEIDSEPEELDRINR
HHHCCCEEHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHH
ELRRLEIEREALKRELEATGSV
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901