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The map label for this gene is xth [H]

Identifier: 21674945

GI number: 21674945

Start: 2020326

End: 2021087

Strand: Reverse

Name: xth [H]

Synonym: CT2136

Alternate gene names: 21674945

Gene position: 2021087-2020326 (Counterclockwise)

Preceding gene: 21674946

Following gene: 21674944

Centisome position: 93.79

GC content: 58.79

Gene sequence:

>762_bases
ATGAATATTTTTACCTGGAACATCAACGGCATCCGCGCCCGAAAGGAGGCGCTTGCCGCGTGGCTCGACAGCCGTAAGCC
GGACATCGTCGTGCTCGAAGAGATCAAGGCGCAGGTGAGCGAGATTCCCGAAACAATCAGGGATTTCGCCGGTTATCGCA
AATTCTGGAACGGTTCGACCTTCAAAAAAGGGTACAGCGGCGTCGGCCTGCTCGTCCGCGACGGCAGCCTCGACGAGTTC
ACCTGCGAGCCGCCACCGTTCGACATCGAGAACCGAACGCTGGTGCTGCACGCCCCGCAGTTTACCCTTATCGGAACCTA
CGTGCCGCGTGGCGACGGGGAGGAACGCTACGCGGTGAAGCTCCGCTATCTTGCCGATCTGAAAGCGTTTATTGCTGAAC
TTCTCACCGAAGGGCGCGAGGTCATTCTCCTTGGCGACATCAACGTGGCGCTTCGCGATATCGACGTGCACCGCTCGCAG
AACAAGCCCGGCGCCATCGGTCTTCGTCCCGAAGAGCGGGCGGCTATCGAGGCGCACCTTGGACTTGGGCTGCTCGACAT
CGTCCGAGAGCTGAATCCCGACAAAAAAGACCTGTTCACCTGGTGGCCCAACTGGAAATTCGCCCGCGAGCGCAATCTCG
GCTGGCGCATCGATTGTATCTATCTTACCCCCGCTCTTGCCAGAAAAGTCTCCGGAGTCTCGGTTGATCTCGACGAGAAA
AGCTCCGACCACGCGCCGGTATCGGTCAAACTCTCTTTGTGA

Upstream 100 bases:

>100_bases
GCCGCGCAGCCTGCCCGAAAGGGCACTTCCGCTGCATGACCGAAATCGGGCCGGAACGCATCGCGAATCGCATCGTTGAA
ACGCTCAATGAGAAATCATC

Downstream 100 bases:

>100_bases
TTGATGGTTGTAAAAAGGCTGTTAACAATGCTGTATTCGAGGCGTTGGAAAACAAAAGAAAAATTTTTACCTTGCGTGTC
CTAAAACGCGAATGGTGTTC

Product: AP Xth family endonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MNIFTWNINGIRARKEALAAWLDSRKPDIVVLEEIKAQVSEIPETIRDFAGYRKFWNGSTFKKGYSGVGLLVRDGSLDEF
TCEPPPFDIENRTLVLHAPQFTLIGTYVPRGDGEERYAVKLRYLADLKAFIAELLTEGREVILLGDINVALRDIDVHRSQ
NKPGAIGLRPEERAAIEAHLGLGLLDIVRELNPDKKDLFTWWPNWKFARERNLGWRIDCIYLTPALARKVSGVSVDLDEK
SSDHAPVSVKLSL

Sequences:

>Translated_253_residues
MNIFTWNINGIRARKEALAAWLDSRKPDIVVLEEIKAQVSEIPETIRDFAGYRKFWNGSTFKKGYSGVGLLVRDGSLDEF
TCEPPPFDIENRTLVLHAPQFTLIGTYVPRGDGEERYAVKLRYLADLKAFIAELLTEGREVILLGDINVALRDIDVHRSQ
NKPGAIGLRPEERAAIEAHLGLGLLDIVRELNPDKKDLFTWWPNWKFARERNLGWRIDCIYLTPALARKVSGVSVDLDEK
SSDHAPVSVKLSL
>Mature_253_residues
MNIFTWNINGIRARKEALAAWLDSRKPDIVVLEEIKAQVSEIPETIRDFAGYRKFWNGSTFKKGYSGVGLLVRDGSLDEF
TCEPPPFDIENRTLVLHAPQFTLIGTYVPRGDGEERYAVKLRYLADLKAFIAELLTEGREVILLGDINVALRDIDVHRSQ
NKPGAIGLRPEERAAIEAHLGLGLLDIVRELNPDKKDLFTWWPNWKFARERNLGWRIDCIYLTPALARKVSGVSVDLDEK
SSDHAPVSVKLSL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=259, Percent_Identity=30.5019305019305, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI18375503, Length=259, Percent_Identity=30.5019305019305, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI18375501, Length=259, Percent_Identity=30.5019305019305, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI18375507, Length=313, Percent_Identity=28.7539936102236, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1788046, Length=265, Percent_Identity=27.5471698113208, Blast_Score=108, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71989536, Length=269, Percent_Identity=30.4832713754647, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI221330655, Length=253, Percent_Identity=30.0395256916996, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17136678, Length=253, Percent_Identity=30.0395256916996, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 28639; Mature: 28639

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIFTWNINGIRARKEALAAWLDSRKPDIVVLEEIKAQVSEIPETIRDFAGYRKFWNGST
CEEEEEECCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FKKGYSGVGLLVRDGSLDEFTCEPPPFDIENRTLVLHAPQFTLIGTYVPRGDGEERYAVK
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEEE
LRYLADLKAFIAELLTEGREVILLGDINVALRDIDVHRSQNKPGAIGLRPEERAAIEAHL
HHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCCCCEECCCCCHHHHHHHHH
GLGLLDIVRELNPDKKDLFTWWPNWKFARERNLGWRIDCIYLTPALARKVSGVSVDLDEK
CCCHHHHHHHCCCCHHHEEEECCCCEEECCCCCCEEEEEEEECHHHHHHHCCCEEECCCC
SSDHAPVSVKLSL
CCCCCCEEEEEEC
>Mature Secondary Structure
MNIFTWNINGIRARKEALAAWLDSRKPDIVVLEEIKAQVSEIPETIRDFAGYRKFWNGST
CEEEEEECCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FKKGYSGVGLLVRDGSLDEFTCEPPPFDIENRTLVLHAPQFTLIGTYVPRGDGEERYAVK
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEEE
LRYLADLKAFIAELLTEGREVILLGDINVALRDIDVHRSQNKPGAIGLRPEERAAIEAHL
HHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCCCCEECCCCCHHHHHHHHH
GLGLLDIVRELNPDKKDLFTWWPNWKFARERNLGWRIDCIYLTPALARKVSGVSVDLDEK
CCCHHHHHHHCCCCHHHEEEECCCCEEECCCCCCEEEEEEEECHHHHHHHCCCEEECCCC
SSDHAPVSVKLSL
CCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]